Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,476,080 G→T 6.7% G215V (GGG→GTG)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,476,0800GT 6.7% 359.2 / 3.3 157G215V (GGG→GTG) yjgLSopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base G (78/63);  new base T (8/5);  total (89/68)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

ATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGT  >  NC_000913/4475932‑4476228
                                                                                                                                                    |                                                                                                                                                    
aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAggg                                                                                                                                                      <  2:1289732/149‑1 (MQ=255)
aTTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAggg                                                                                                                                                      <  1:983082/149‑1 (MQ=255)
    cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTa                                                                                                                                                  >  2:1563206/1‑149 (MQ=255)
    cTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTAAAGAAGTTAAAAAATCTACTGAAGTTAAATAATATACAGGGTTTa                                                                                                                                                  >  1:1079625/1‑149 (MQ=255)
     tAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaa                                                                                                                                                 <  1:792234/149‑1 (MQ=255)
     tAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGGCTATTAAATAATAATCAGGAGTTAAATACTCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGTGTTaa                                                                                                                                                 >  1:132959/1‑149 (MQ=255)
      aaCAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCAACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTaaa                                                                                                                                                <  2:455609/149‑1 (MQ=255)
         aaGGTGATGGCGTGTTACCATAGTGAAGCTATCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAaataa                                                                                                                                             <  2:908690/149‑1 (MQ=255)
           tggtatGGCGTGTTAACCAAGTGAATCTAACAATAAACTTGATTGTGTATTAAATAATAATCAGTTGTTAAATAATCTACATAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATc                                                                                                                                           <  2:1463122/144‑1 (MQ=255)
           ggTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATc                                                                                                                                           <  2:626695/149‑1 (MQ=255)
             ttCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCt                                                                                                                                         >  1:340471/1‑149 (MQ=255)
               cttgCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCa                                                                                                                                       <  2:631429/146‑1 (MQ=255)
                 ggCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAga                                                                                                                                     >  2:1350585/1‑148 (MQ=255)
                  gCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCATCAGGa                                                                                                                                    >  2:235871/1‑149 (MQ=255)
                    gtgtTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTTCAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGt                                                                                                                                  <  2:1538933/149‑1 (MQ=255)
                    gtgtTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGt                                                                                                                                  <  2:88983/149‑1 (MQ=255)
                    gtgtTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGCTTTAAATAATCCTCAGGAGt                                                                                                                                  >  2:106284/1‑149 (MQ=255)
                       ttACCAAAGTGAAGCTAACAAGAAAATAGCGCGTATATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATTATATACAGGCGTTAAATAATCCTCAGGAGTTaa                                                                                                                               >  2:139206/1‑149 (MQ=255)
                         aCCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaat                                                                                                                             >  2:923618/1‑149 (MQ=255)
                           cAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAaataa                                                                                                                           >  1:968306/1‑149 (MQ=255)
                               gTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGTGGTTAAATAATCCTCAGGAGTTAAATAATCCg                                                                                                                       >  1:442229/1‑149 (MQ=255)
                                 gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGTTTAAATAATCCTCAGGAGTTAAATAATCCgaa                                                                                                                     >  1:1520594/1‑147 (MQ=255)
                                 gAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAAAATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCa                                                                                                                     >  2:1194028/1‑149 (MQ=255)
                                  aaGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAg                                                                                                                    >  2:939997/1‑149 (MQ=255)
                                       aaCAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                               <  2:151202/149‑1 (MQ=255)
                                          aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                               <  1:709356/146‑1 (MQ=255)
                                          aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAAttt                                                                                                               >  2:709356/1‑146 (MQ=255)
                                          aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                                                                            >  1:75014/1‑149 (MQ=255)
                                          aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTaaa                                                                                                            >  2:1502348/1‑149 (MQ=255)
                                          aaGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCAACAGAAATAAAATAATCTACAGAAGTTAAATAATCAACTGAAGTTAAATAATATACATGTGTTAAATAATACTCAGGAGTTAAATAATCAGCAGAATTTaaa                                                                                                            >  1:688782/1‑149 (MQ=255)
                                           aGAAAATAGAGCGTCAATTAAATAATAATCAGTAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAAt                                                                                                           <  1:1488978/149‑1 (MQ=255)
                                             aaaaTAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGATAAATAATCCTCAGAATTTAAATGa                                                                                                         >  1:148026/1‑149 (MQ=255)
                                               actAGCGCGTCTATTAAATAATATTCATGATTTAAATAATCTTCAGAAATTAAATAATCTACATAATTTAAATAATCTACTGAAGTTAAATAATCTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGAtt                                                                                                       <  2:842836/147‑1 (MQ=255)
                                                   gcgcGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCATCAGGAGTTAAATAATACGCAGAATTTAAATGATTCTCa                                                                                                   >  1:1052863/1‑149 (MQ=255)
                                                     gcgTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGTTTAAATAATCATCAGGAGTTAAATAATCAGCAGAATTTAAATGATTCTCAgg                                                                                                 >  1:918839/1‑149 (MQ=255)
                                                     gagTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAgg                                                                                                 <  1:624267/147‑1 (MQ=255)
                                                       gTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAg                                                                                               >  1:559814/1‑149 (MQ=255)
                                                         cTATTAAATAATAATCAGGAGTTAAATATTCTAAAGATATTAAATAATCTAGAGAAGTTAAATTATCTACTGATCTTAAATAATATACAGGGGTTATATAATCCTCAGGAGTTAAATAATCCGCAGAATTTATATGATTCTCAGGAGtt                                                                                             <  2:1138911/149‑1 (MQ=255)
                                                         cTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTAAAATAATCCGCAGAATTTAAATGATTCTCAGGAGtt                                                                                             >  1:876048/1‑149 (MQ=255)
                                                          tATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTa                                                                                            <  2:696178/149‑1 (MQ=255)
                                                           aTTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaa                                                                                           <  1:375517/149‑1 (MQ=255)
                                                            ttAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                                                                                          <  2:135178/149‑1 (MQ=255)
                                                            ttAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTaaa                                                                                          <  2:690626/149‑1 (MQ=255)
                                                               aataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTTAATAATATTCAGGGGTTAAATAATCCTCATGAGTTAAATAATCCTCAGAATTTAAATAATTCTAAGTAGTTAAATaa                                                                                       >  1:962779/1‑149 (MQ=255)
                                                               aataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAATTAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATaa                                                                                       >  1:1119391/1‑149 (MQ=255)
                                                               aataataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAAGATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATaa                                                                                       >  1:1271665/1‑149 (MQ=255)
                                                                 taataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACt                                                                                     >  2:1375397/1‑149 (MQ=255)
                                                                  aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTc                                                                                    <  2:1384479/149‑1 (MQ=255)
                                                                  aataatCAGGAGTTAAATAATCTACAGAAATTAAATAATCAACAGAAGTTAAATAAACAACTGAAATTAAATAATAAACAGGATTTAAATAAAAATCAGGAGTTAAATAATCAGCATAATTAAAATCATTATCACGAGTAAAATAACTc                                                                                    >  2:975912/1‑149 (MQ=255)
                                                                    taatCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGc                                                                                  <  1:877419/149‑1 (MQ=255)
                                                                       tCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAgt                                                                               >  1:1335354/1‑148 (MQ=255)
                                                                        cAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGTTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCATGAGTTAAATAACTCGAAGGa                                                                              >  2:380247/1‑149 (MQ=255)
                                                                          ggAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGCAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGGAGGAAt                                                                            >  2:143408/1‑149 (MQ=255)
                                                                          ggAGTTAAATAAAATACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAAt                                                                            <  2:1101843/149‑1 (MQ=255)
                                                                              ttAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTaaa                                                                        >  1:1153111/1‑149 (MQ=255)
                                                                               tAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTAGCAGGAATTatat                                                                       >  2:1194939/1‑149 (MQ=255)
                                                                                     atCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCca                                                                 >  1:649930/1‑149 (MQ=255)
                                                                                       cTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCcaca                                                               >  1:149762/1‑149 (MQ=255)
                                                                                        tACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAg                                                              <  1:375298/149‑1 (MQ=255)
                                                                                        tACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCAGCAGAATTTAAATTATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCAACAg                                                              >  1:931858/1‑149 (MQ=255)
                                                                                             aaaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGtt                                                         <  1:1563206/149‑1 (MQ=255)
                                                                                             aaaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATAGACAGGGGTTAAATAATCCTCATGAGTTAAATAATCCGAAGAATTTAAATGATTCTCAGGAGTTAAATAACACGCAGTAATTAAATAGACCACAGGAGtt                                                         >  1:1038060/1‑149 (MQ=255)
                                                                                              aaTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCGCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGTAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTa                                                        >  2:712010/1‑149 (MQ=255)
                                                                                               aTTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGTGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATCATTCTCAGCAGTAAAATAACTAGAAGGAATTAAATAATCCACATGAGTaaa                                                       >  2:1444606/1‑149 (MQ=255)
                                                                                                ttaattaaTCTACAGTATTTAATTAATCTACTGAAGTTAAATAATATAGAGTTGTTAAATAATCCTCAGTAGTTAAATAATCCTCATAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTaaa                                                      <  1:975912/149‑1 (MQ=255)
                                                                                                 tAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAAt                                                     <  2:270746/149‑1 (MQ=255)
                                                                                                     taatCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATc                                                 >  2:191676/1‑149 (MQ=255)
                                                                                                       atCTACAGAATTTAAATAATATAATTAATTTAAATAATATACATGGGTTAAATAATACTCATGAGTTAAATAATCCTCAGAATTTAAATTATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCg                                               <  1:1194028/149‑1 (MQ=255)
                                                                                                               aaGTTAAATAATCTACTTAAGTTAAATAATATACCTGTTTTAAATAATCCTCAGGATTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                       <  2:968306/149‑1 (MQ=255)
                                                                                                               aaGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGtt                                       >  1:1551350/1‑149 (MQ=255)
                                                                                                                aGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCAGCAGGAGTTa                                      >  2:330227/1‑149 (MQ=255)
                                                                                                                 tttAAATAATCTACTGAAGTTAAATAATATACAGTGGTTAAATAATACTCATGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTaa                                     <  1:1027621/148‑1 (MQ=255)
                                                                                                                 tttAAATAATCTACTGAAGTTAAATAATATACAGGTTTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTaa                                     <  1:235818/148‑1 (MQ=255)
                                                                                                                    aaaTAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAAATCGCAGGAATTAAATTGTCAAAATGAGTTAAATTATACGCAGGAGTTAaata                                  >  1:1377729/1‑149 (MQ=255)
                                                                                                                    aaaTAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAAACCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTAGAAGGAATTAAATAGTCCAAAGGAGTTAAATGATCCGCAGGAGTTAaata                                  >  2:509275/1‑149 (MQ=255)
                                                                                                                      ataatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAaataat                                >  2:1163186/1‑149 (MQ=255)
                                                                                                                       taatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCTCAGGAGTTATATAAtt                               >  1:610337/1‑149 (MQ=255)
                                                                                                                       taatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAAtt                               <  2:1260878/149‑1 (MQ=255)
                                                                                                                        aatCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCATGAGTTAAATAACTCTAAGGAATTAAATAGTCAACAGGAGTTAAATGATCCGCAGGAGTTAAATAAtta                              >  1:702876/1‑148 (MQ=255)
                                                                                                                         atCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATtct                             >  2:260786/1‑149 (MQ=255)
                                                                                                                          tCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTAAAATAACTCGCAGTAATTAATTAGTCCAAAGGAATTAAATGAACCGCAGGATTTAATTAAAtcac                            >  2:1424903/1‑147 (MQ=255)
                                                                                                                           cTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCa                           <  1:1367450/149‑1 (MQ=255)
                                                                                                                           cTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCa                           <  2:1309806/149‑1 (MQ=255)
                                                                                                                              cTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGa                        <  1:928089/149‑1 (MQ=255)
                                                                                                                                 aaGTTAAATAATCTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGACTTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACtt                     <  2:1052863/149‑1 (MQ=255)
                                                                                                                                 aaGATAAATTATATACAGTGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATATAACTGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGTGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGTACtt                     <  1:208946/149‑1 (MQ=255)
                                                                                                                                  aGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTa                    >  1:1344372/1‑149 (MQ=255)
                                                                                                                                    ttAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGTACTTaaa                  >  1:581743/1‑149 (MQ=255)
                                                                                                                                    ttAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTaaa                  >  2:560940/1‑149 (MQ=255)
                                                                                                                                       aataatATACATGGGTTAAATAATCATCATGATTTAAATAATCAGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATaa               <  1:235871/149‑1 (MQ=255)
                                                                                                                                       aataatATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATaa               <  1:419403/149‑1 (MQ=255)
                                                                                                                                        ataataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGATTTTAAATGATTCTCAGGAGTTAAATAACTCGGAGGAATTAAATAGTCCACAGGATTTAAATAAACCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAc              >  1:1140999/1‑149 (MQ=255)
                                                                                                                                         taataTACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAAct             <  1:476886/149‑1 (MQ=255)
                                                                                                                                             ataCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTATCAGGACTTAAATAAATCTaa         >  2:428673/1‑149 (MQ=255)
                                                                                                                                              ttaagtggTTAAATAATCCTCAGGAGTTAAATAATCAGCAGAATTTAAATGATTCTCAGTAGTTAAATAACTCGCAGGAATTAAATAGTCCACATGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAg        <  1:509275/143‑1 (MQ=255)
                                                                                                                                              taCAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAg        <  1:563574/149‑1 (MQ=255)
                                                                                                                                                 aGGGGATAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATACTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACACGACTTAAATGATCCGAAGGAGTAAAATAATTATCAGGACTTAAATAACTATAAGGTg     >  1:1201814/1‑149 (MQ=255)
                                                                                                                                                   gggTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAg   >  2:1333158/1‑149 (MQ=255)
                                                                                                                                                   gggTTAAATAATCCTCAGGAGTTAAATAATCATCAGAATTTAAATTATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAg   <  1:106284/149‑1 (MQ=255)
                                                                                                                                                   gggTTAAATAATCATCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAg   <  1:691115/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTACTAACAAGGTTCTGGCGTGTTACCAAAGTGAAGCTAACAAGAAAATAGCGCGTCTATTAAATAATAATCAGGAGTTAAATAATCTACAGAAATTAAATAATCTACAGAAGTTAAATAATCTACTGAAGTTAAATAATATACAGGGGTTAAATAATCCTCAGGAGTTAAATAATCCGCAGAATTTAAATGATTCTCAGGAGTTAAATAACTCGCAGGAATTAAATAGTCCACAGGAGTTAAATGATCCGCAGGAGTTAAATAATTCTCAGGACTTAAATAACTCTAAGGTGAGT  >  NC_000913/4475932‑4476228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: