Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,484,461 A→C 5.7% A497A (GCT→GCG pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,484,4610AC 5.7% 289.9 / 4.8 120A497A (GCT→GCGpepAmultifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
Reads supporting (aligned to +/- strand):  ref base A (59/53);  new base C (6/2);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 121 total aligned reads displayed.

CAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCAACCTGCGGTAA  >  NC_000913/4484316‑4484609
                                                                                                                                                 |                                                                                                                                                    
ccggTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgcg                                                                                                                                                   <  2:854604/147‑1 (MQ=255)
cAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAGCCTAGCCTGACGCAATTACTCTTCGCCGTTAAACCCAgcg                                                                                                                                                   >  2:1201724/1‑149 (MQ=255)
  ggTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGcgcg                                                                                                                                                 <  1:784139/149‑1 (MQ=255)
   gTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCgg                                                                                                                                                >  1:522794/1‑149 (MQ=255)
   gTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCgg                                                                                                                                                <  1:1544764/149‑1 (MQ=255)
    tataCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGt                                                                                                                                               <  2:1117877/149‑1 (MQ=255)
    tataCACCGCAGCGCCTGATGCGACGCTGGTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATACGGCGTTCACGCCGCATCCGGCCATACCAGCCTTGCCTGCCGCTATTACTCGTCGCCGTTACACCCAGCGCGGt                                                                                                                                               >  2:1196961/1‑149 (MQ=38)
     ataCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGtt                                                                                                                                              >  2:695562/1‑149 (MQ=255)
     ataCACCGCAGCGCCTGATGCGAAGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGGAATAACAGCCTTGCCTGACGAAATTACTCTTCGCCGTTAAACCCAGCGCGGtt                                                                                                                                              <  2:790795/149‑1 (MQ=255)
      taCACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAACCCCAGCGCGGttt                                                                                                                                             <  1:1215210/149‑1 (MQ=255)
        cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTaa                                                                                                                                           >  2:510542/1‑149 (MQ=255)
        cacCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTaa                                                                                                                                           <  1:116492/149‑1 (MQ=255)
        cacCGCAGCGCCTGATGCGACGCTGTTGCGTCTTACCCGGCCTACGAGGTCAGTGCAGGGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTaa                                                                                                                                           <  2:712871/149‑1 (MQ=38)
         acCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATATCAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCGCGCTGGGTTTAAc                                                                                                                                          <  1:1092889/149‑1 (MQ=38)
         acCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGAAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAAc                                                                                                                                          >  1:1083266/1‑149 (MQ=255)
              aGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTACGCCGTTAAACCAAGCGCGGTTTAACAGGaa                                                                                                                                     >  1:1412287/1‑149 (MQ=255)
               gcgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAAc                                                                                                                                    <  2:757575/149‑1 (MQ=255)
                cgcCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATAACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACt                                                                                                                                   <  1:988910/149‑1 (MQ=255)
                  ccTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACtgt                                                                                                                                 <  2:397682/149‑1 (MQ=255)
                     gATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGcc                                                                                                                              >  2:812230/1‑149 (MQ=255)
                     gATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTGCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGcc                                                                                                                              <  2:38988/149‑1 (MQ=255)
                     gATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGGTCAGTGCGGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGGTTAACAGGAACTGTGcc                                                                                                                              >  2:885093/1‑149 (MQ=255)
                       tGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAg                                                                                                                            >  2:833580/1‑149 (MQ=255)
                          gACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCaa                                                                                                                         >  2:1362402/1‑149 (MQ=255)
                          gACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCaa                                                                                                                         >  1:17895/1‑149 (MQ=255)
                           aCGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAAc                                                                                                                        >  1:1113983/1‑149 (MQ=255)
                           aCGCTGTGGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAAc                                                                                                                        <  2:60590/149‑1 (MQ=255)
                                 ttgtGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTAcc                                                                                                                  <  1:1224692/149‑1 (MQ=255)
                                        ttATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGgacgac                                                                                                           <  2:1505887/149‑1 (MQ=255)
                                          aTCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGCAACTGTGCCAGCAACGCTACCGGACGACCg                                                                                                         >  1:718534/1‑149 (MQ=255)
                                           tCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCgg                                                                                                        <  1:1322909/149‑1 (MQ=255)
                                              ggCCTACGAGTTCAGTGCTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCCATAACAGCCTTGCCTGACGCAATTACTCTTCTCCGTTAAACCCAGCGCGGTTGAACAGGAACTGTGCAAGCAACGCTACCGGACGACCGGAgg                                                                                                     >  1:493434/1‑149 (MQ=38)
                                              ggCCTACGAGGTCAGTGCTGTGGAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGGTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTgg                                                                                                     <  2:394446/149‑1 (MQ=255)
                                               gCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGgc                                                                                                    >  2:837391/1‑149 (MQ=255)
                                               gCCTACGAGTTCAGGGCTGTGTAGGTCGGATACGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTTgc                                                                                                    <  1:1316448/149‑1 (MQ=255)
                                                 cTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGgcgc                                                                                                  >  1:1272885/1‑149 (MQ=255)
                                                     gAGTTCAGTGCTGTGTAGGTCGGATAAGGCATTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCttt                                                                                              <  2:833699/149‑1 (MQ=255)
                                                       gTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTg                                                                                            <  2:369382/149‑1 (MQ=255)
                                                       gTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTg                                                                                            <  2:1099662/149‑1 (MQ=255)
                                                          cAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCCACGCTACCGGACGCCAGCTGGCGCCTTTTGCtt                                                                                         >  1:913527/1‑149 (MQ=255)
                                                          cAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCtt                                                                                         <  2:576426/149‑1 (MQ=255)
                                                            gTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTa                                                                                       >  1:522496/1‑149 (MQ=255)
                                                             tGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAGCCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTAc                                                                                      >  1:1555553/1‑149 (MQ=255)
                                                             tGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGCCGACCGGTGGCGCCTATTGCTTTAc                                                                                      >  2:1388845/1‑149 (MQ=255)
                                                             tGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTAc                                                                                      <  1:1485243/149‑1 (MQ=255)
                                                              gCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTAcc                                                                                     >  1:1156068/1‑149 (MQ=255)
                                                              gCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTAcc                                                                                     <  1:1371229/149‑1 (MQ=255)
                                                               cTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCa                                                                                    >  1:1106707/1‑149 (MQ=255)
                                                                  tgtAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGaa                                                                                 <  2:522794/149‑1 (MQ=255)
                                                                  tgtAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGaa                                                                                 >  1:97345/1‑149 (MQ=255)
                                                                   gtAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAAc                                                                                <  2:599/149‑1 (MQ=255)
                                                                   gtAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCACGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTGCCGGACGCCCGGTGGCGCCTTTTGCTTTACCAGAAc                                                                                >  2:881200/1‑149 (MQ=255)
                                                                      tgTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACTGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCGTTTTGCTTTACCATAACGcc                                                                             <  2:1108407/148‑1 (MQ=255)
                                                                      ggTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGcc                                                                             <  1:1020059/149‑1 (MQ=255)
                                                                       gTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCa                                                                            >  1:581143/1‑149 (MQ=255)
                                                                       gTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCa                                                                            >  2:951637/1‑149 (MQ=255)
                                                                          ggATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGc                                                                         <  2:204650/149‑1 (MQ=255)
                                                                          ggATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGc                                                                         <  1:419416/149‑1 (MQ=255)
                                                                            aTAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAACCCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCgg                                                                       >  1:822863/1‑149 (MQ=255)
                                                                            aTAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCgg                                                                       <  2:80280/149‑1 (MQ=255)
                                                                                  cGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCgg                                                                 <  2:767024/149‑1 (MQ=255)
                                                                                  cGTGCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCgg                                                                 <  1:747317/149‑1 (MQ=255)
                                                                                   gTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGc                                                                <  2:97345/149‑1 (MQ=255)
                                                                                     tCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGAGCCAGCCACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGa                                                              <  1:1388845/149‑1 (MQ=255)
                                                                                          ccgcATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATcc                                                         <  2:220312/149‑1 (MQ=255)
                                                                                             cATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAgg                                                      <  1:135966/149‑1 (MQ=255)
                                                                                              aTCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGt                                                     >  1:466038/1‑149 (MQ=255)
                                                                                                ccGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCAAGGCGGTACCGGCGATATCCAGGTgc                                                   >  1:891714/1‑149 (MQ=255)
                                                                                                  ggCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCCGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTgcgc                                                 >  2:261856/1‑149 (MQ=255)
                                                                                                  ggCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTgcgc                                                 <  2:1225173/149‑1 (MQ=255)
                                                                                                   gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGcc                                                >  2:1080801/1‑149 (MQ=255)
                                                                                                   gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGcc                                                <  2:1106707/149‑1 (MQ=255)
                                                                                                   gCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGcc                                                >  2:594888/1‑149 (MQ=255)
                                                                                                        aaCAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGtt                                           <  2:1113983/149‑1 (MQ=255)
                                                                                                          cAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCCGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGTCCGTACGGGGGATTTCCAGGTGCGccgcggtgt                                         >  2:317926/1‑142 (MQ=255)
                                                                                                          cAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGAGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGACAAGTTGt                                         >  2:1559544/1‑149 (MQ=255)
                                                                                                             ccTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACt                                      >  1:625407/1‑149 (MQ=255)
                                                                                                               ttGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGAAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTa                                    >  2:1372425/1‑149 (MQ=255)
                                                                                                                   cTGACGCAATTACTCTTCGCCGTTAAACCCAGAGCTGTTTACCAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGGCTTTTGCTTTACCAGAACGCCAGGCGGAACCGTCGATATACAGGAGCTCCAAGTTGTACTTTCggg                                >  2:857349/1‑149 (MQ=255)
                                                                                                                    tGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACTCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATCTCCAGGTGCGCCCAGTGGTAGTTACgggg                               >  2:118317/1‑148 (MQ=255)
                                                                                                                      aCGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTaa                             >  2:1241127/1‑149 (MQ=255)
                                                                                                                          aaTTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCg                         >  2:606855/1‑149 (MQ=255)
                                                                                                                          aaTTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGGGCCAGCAACGCTACCGGACGACCGGTGGCGCCATTTGCTTTACCAGAACGCCAGGAGGTACCGGCGATATCCAGGTGCGCCCCGTTGTACTTACGGGTAAAGCg                         >  1:695104/1‑149 (MQ=255)
                                                                                                                             tACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGGGCGCCCAGTTGTACTGACGGGTAAAGCGTgc                      >  1:437918/1‑148 (MQ=255)
                                                                                                                                 ctTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGCACGCCAGGCGGTACCGGCGATATCCAGGGGCGCCCAGTTGTACTTGCGGGTAAAGCGTGACAgg                  >  1:790284/1‑149 (MQ=255)
                                                                                                                                 ctTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAgg                  >  1:178407/1‑149 (MQ=255)
                                                                                                                                  ttCGCCGTTAACCCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTCCGGGTAACGCGTGACAGGa                 >  2:85282/1‑149 (MQ=255)
                                                                                                                                    cGCCGTTAAACCCCGCGCGGTTTAACAGGAACTGTGCCAGCACCGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAg               <  1:1303802/149‑1 (MQ=255)
                                                                                                                                     gCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCCGGCGGTACCGGCGATATCCAGGTGCGCCCCGTAGTACTTACGGGTAAAGCGTGCCAGGAAGc              >  2:839511/1‑149 (MQ=255)
                                                                                                                                     gCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGc              <  2:522496/149‑1 (MQ=255)
                                                                                                                                     gCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGc              >  2:135644/1‑149 (MQ=255)
                                                                                                                                      ctgTTAAACCGCGCTGGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGCCCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGCCAGGAAGCa             >  1:470009/3‑149 (MQ=255)
                                                                                                                                      ccGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCa             >  2:814706/1‑149 (MQ=255)
                                                                                                                                       tgTTAAACCGCGCTGGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCaa            >  1:1465430/2‑149 (MQ=255)
                                                                                                                                       tgTTAAACCGCGCTGGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCaa            >  1:1338859/2‑149 (MQ=255)
                                                                                                                                       cGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCaa            >  2:1036004/1‑149 (MQ=255)
                                                                                                                                             aCCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCACCCTGCg      >  1:580784/1‑149 (MQ=255)
                                                                                                                                               ccAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGGCTTTTTCTTTACCAGAACTCCAGGCGGTACCGGCCATATCCAGGTGGGCACTGATGTAGTTACGGTAATAGCGTGTCTGGACGAAGCCTTCggg    >  2:1011923/1‑148 (MQ=255)
                                                                                                                                               ccAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCAACCTGCGGt    <  1:86511/149‑1 (MQ=255)
                                                                                                                                               ccAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCAACCTGCGGt    >  2:578296/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAGGTATACACCGCAGCGCCTGATGCGACGCTGTTGTGTCTTATCAGGCCTACGAGTTCAGTGCTGTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGGCAATAACAGCCTTGCCTGACGCAATTACTCTTCGCCGTTAAACCCAGCGCGGTTTAACAGGAACTGTGCCAGCAACGCTACCGGACGACCGGTGGCGCCTTTTGCTTTACCAGAACGCCAGGCGGTACCGGCGATATCCAGGTGCGCCCAGTTGTACTTACGGGTAAAGCGTGACAGGAAGCAACCTGCGGTAA  >  NC_000913/4484316‑4484609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: