Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,588 T→C 5.0% intergenic (‑45/+32) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5880TC 5.0% 262.5 / 4.7 100intergenic (‑45/+32)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (52/42);  new base C (2/3);  total (55/45)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

TGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAG  >  NC_000913/265441‑265735
                                                                                                                                                   |                                                                                                                                                   
tGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTc                                                                                                                                                    >  2:2899/1‑149 (MQ=255)
  cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGCGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCAAGCTGTCgg                                                                                                                                                  >  2:692453/1‑149 (MQ=255)
   ccTGGTCGTCTTCGAGTGTGATCTGGCTGTACGTGGTCGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                                 <  2:56767/149‑1 (MQ=255)
   ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCATCACGGATTGGGTCATTTTCGGGCGCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                                 <  1:713651/149‑1 (MQ=255)
   ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                                 <  2:1187565/149‑1 (MQ=255)
   ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                                 <  1:401421/149‑1 (MQ=255)
   ccTGGTCGTCTTCGAGGGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                                 <  1:1543464/149‑1 (MQ=255)
    cTGGTCGGCTTCGAGTGTGAAATTGCTGGACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAACGGCCCCGGTGGCAGCAACACGCATTGCGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTGGCTGTCgggg                                                                                                                                                <  2:743377/149‑1 (MQ=255)
       gtcgtcTTCGAGTGTGATACTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGa                                                                                                                                             >  1:1440896/1‑149 (MQ=255)
        gcgtcTTCGAGTGTGATATTGCTGTACGTTGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCCTTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGaa                                                                                                                                            <  2:1117662/148‑1 (MQ=255)
         cgtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAt                                                                                                                                           <  2:498488/149‑1 (MQ=255)
             ttCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                                       >  2:942558/1‑149 (MQ=255)
               cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGTATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                                     <  1:1394416/149‑1 (MQ=255)
               cGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                                     <  2:1536536/149‑1 (MQ=255)
                    gtgATATTGCTCTACGTGGTAGTGTCCGCTTGCGCTTGTCTCCGGGGGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGCCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGaa                                                                                                                                <  1:1082177/149‑1 (MQ=255)
                     tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAg                                                                                                                               >  1:943905/1‑149 (MQ=255)
                         atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtac                                                                                                                           >  1:260371/1‑149 (MQ=255)
                           tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                         <  1:962664/149‑1 (MQ=255)
                           tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCGCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAACCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                         <  1:501974/149‑1 (MQ=255)
                             cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                                       >  1:460909/1‑149 (MQ=255)
                             cTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATactact                                                                                                                       <  2:298711/149‑1 (MQ=255)
                                gtcGTGTTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATc                                                                                                                    <  1:291984/147‑1 (MQ=255)
                                          tGACCGCTTGAGCTTGGCTCCGGGTGAAAGGCCCCTTTGGCAGCAACACGGTTTGGGTCATTTTCGGGCTCCTGATAAAACAAAACCCAGGCAGCCTGCTATCTGTCGTGGTGAATTTGCTGGGGAAGATACTACTATCAGGCGTtgct                                                                                                          <  1:178790/149‑1 (MQ=255)
                                            aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACAGGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                        >  1:591501/1‑149 (MQ=255)
                                            aCCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                        >  2:1183322/1‑149 (MQ=255)
                                             ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGTTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                                       <  2:182810/149‑1 (MQ=255)
                                             ccGCTTGCGCTTGGCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCa                                                                                                       <  2:414699/149‑1 (MQ=255)
                                               gcttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCACCACGGAGTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGt                                                                                                     >  1:1004790/1‑149 (MQ=255)
                                                cttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtc                                                                                                    >  1:813151/1‑149 (MQ=255)
                                                  tgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCACCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtctc                                                                                                  <  2:669211/149‑1 (MQ=255)
                                                   gcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAGAGAAAACCCCGGCAGCCGGCGAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTcc                                                                                                 >  1:708628/1‑149 (MQ=255)
                                                   gcgcttgTCGCCGGGTGACAGGCCCCGGTGGCAGCAACACGGCTTGGGGCATTTTCGGGCTCCTGATAAAAGCAAACCCCGGCAGCCAGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGGTGCTGCAGTCTcc                                                                                                 <  2:1396638/149‑1 (MQ=255)
                                                     gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCga                                                                                               >  2:1561238/1‑149 (MQ=255)
                                                          tctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                                          >  1:620399/1‑149 (MQ=255)
                                                          gctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTg                                                                                          <  1:442185/148‑1 (MQ=255)
                                                            tcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                                        >  1:231888/1‑149 (MQ=255)
                                                              cGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACa                                                                                      <  2:829861/149‑1 (MQ=255)
                                                               gggTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAg                                                                                     >  2:773528/1‑149 (MQ=255)
                                                                 gggAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                                   <  1:380499/147‑1 (MQ=255)
                                                                 gTGAAAGGCCCCGGTGGCAGCAACACGGATGGGGTCATTTTCGGGCGCCTGATAACAGAAACCACCGGCCGCCAGCTCGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaa                                                                                   <  1:856161/149‑1 (MQ=255)
                                                                  tGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGaaa                                                                                  >  2:1496520/1‑148 (MQ=255)
                                                                  ggAAAGGCCCCGGTGGAAGCATCACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAAc                                                                                  <  1:415261/148‑1 (MQ=255)
                                                                   gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACt                                                                                 >  2:740859/1‑149 (MQ=255)
                                                                   gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGCCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTTCTGCAGTCTCCGAGAGTGGACAGAACt                                                                                 >  2:490013/1‑149 (MQ=255)
                                                                    aaaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACtt                                                                                >  2:1360474/1‑149 (MQ=255)
                                                                         ccccGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCATCCTGCTAGCTTTCGGGGTGAATATGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTGTCTCa                                                                           >  2:392614/1‑149 (MQ=255)
                                                                              gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTACCTGTCGGCGTGAATTTGCTGGGGAAGCTACTACTATCAGTCGTTGCTGCAGTCTCCGAGCGTGGACAGCACTTTCTCAGCGtt                                                                      >  1:1051286/1‑149 (MQ=255)
                                                                                    gctccccGGATTGGGGCATTTTCGGGCGCCTGATAAACGCAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCg                                                                <  2:60728/143‑1 (MQ=255)
                                                                                       acacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTc                                                             <  2:1506924/149‑1 (MQ=255)
                                                                                        cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGCCAGACCTTTCTCAGCGTTCTTCCGGTcc                                                            >  2:848036/1‑149 (MQ=255)
                                                                                        cacGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTcc                                                            <  2:599465/149‑1 (MQ=255)
                                                                                           ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTTTCGGGGTCAATATGCTGGGGAAGATACAACTATCAATCGTTTCTGCAGTCTCCGACAGTGGACAGAACATTCTCAGCGTTCTTCCgcacagcc                                                         >  2:1452361/1‑142 (MQ=255)
                                                                                           ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                                         >  1:314936/1‑149 (MQ=255)
                                                                                           ggATTGGGCCATTTTCGGGCGCCTGATAAAATAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCCTTGCTGCAGTCTCCGAGATTGGACTGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                                         <  2:1174771/149‑1 (MQ=255)
                                                                                              ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGATGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                                      >  1:963019/1‑149 (MQ=255)
                                                                                              ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGGCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTg                                                      <  1:223745/149‑1 (MQ=255)
                                                                                                gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCTCAGTGaa                                                    <  2:616099/149‑1 (MQ=255)
                                                                                                gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCACAGCGTTCTTCCGGTCCGCAGTgca                                                    >  2:1349865/1‑147 (MQ=255)
                                                                                                 gggcATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                                   <  2:1175254/146‑1 (MQ=255)
                                                                                                 ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                                   >  2:774560/1‑149 (MQ=255)
                                                                                                 ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                                   >  2:476549/1‑149 (MQ=255)
                                                                                                 ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                                   >  1:1480350/1‑149 (MQ=255)
                                                                                                 ggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                                   >  2:836620/1‑149 (MQ=255)
                                                                                                     cttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcc                                               <  2:957383/148‑1 (MQ=255)
                                                                                                      ttttCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAACAGTCGTTGCTGCAGTCGCCGAGAGTGGCCAGACGTTTGTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                                              >  1:488286/1‑149 (MQ=255)
                                                                                                      ttttCGGGCTCCTGATAAAAGAAAACCACGGCAGCCTGCTAGCTGTCGGGGGGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGTGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTccc                                              >  1:868041/1‑149 (MQ=255)
                                                                                                       tttCGGGCTCCTGATAAAAGAAAACCCCGTCCGCCTGCTAGCTGTCGGGGTGAATTTTCTGGGGAAGATACGACTTTCAGTCGTTGCTGCAGTCTCTGAGAGTGGGCAGCACGTTCTCAGCGTTCGTCCGGTCCGCAGTGAAGGTCCCt                                             >  2:1237896/1‑149 (MQ=255)
                                                                                                         tCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGc                                           <  1:1426041/149‑1 (MQ=255)
                                                                                                             gCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtg                                       >  2:1062958/1‑149 (MQ=255)
                                                                                                              cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                                      <  2:604664/149‑1 (MQ=255)
                                                                                                              cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgt                                      >  1:21918/1‑149 (MQ=255)
                                                                                                               tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                                     <  2:260371/149‑1 (MQ=255)
                                                                                                                ccTGAGAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                                    >  1:327430/1‑149 (MQ=255)
                                                                                                                 cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtgtg                                   >  2:1404951/1‑147 (MQ=255)
                                                                                                                 cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTAATATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCGCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGt                                   >  2:846774/1‑149 (MQ=255)
                                                                                                                  tGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTAGCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTc                                  <  2:267818/149‑1 (MQ=255)
                                                                                                                   gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCa                                 <  1:2899/149‑1 (MQ=255)
                                                                                                                     aaaaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAtt                               <  2:620989/148‑1 (MQ=255)
                                                                                                                        aaGAAAACCCTGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGATCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                            >  1:483090/1‑149 (MQ=255)
                                                                                                                        aaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                            >  1:1198436/1‑149 (MQ=255)
                                                                                                                         cgAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTCTCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGTACTTTCTCAGCGTTCTTCCGGGCCGCTGTGAAGGTCCCTGCTTTGTGGTCATTGACg                           <  2:952455/148‑1 (MQ=255)
                                                                                                                         aGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGACTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACg                           >  2:912286/1‑149 (MQ=255)
                                                                                                                          gAAAACCCCGGCAGCCTGCTTGCTGTCGGGGTGAATTTGCTGGGGAAGATTCTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt                          >  1:1422935/1‑149 (MQ=255)
                                                                                                                             aaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGACCTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACTTAGa                       >  1:698981/1‑149 (MQ=255)
                                                                                                                              aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGCACTTTCACAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc                      >  1:1014540/1‑149 (MQ=255)
                                                                                                                              aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc                      >  2:1471458/1‑149 (MQ=255)
                                                                                                                               ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTACCGGTCCGCAGTGAAGGTCCCTGATTAGTGGTCATTGACGTAGCCg                     >  2:1032556/1‑149 (MQ=255)
                                                                                                                                cccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt                    >  1:923105/1‑149 (MQ=255)
                                                                                                                                  cGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg                  >  2:978915/1‑149 (MQ=255)
                                                                                                                                    gccgCCTGCTAGCTGTCGGGGAGAATTTGCTGGGGAAGATACTAGTATCAGGCGTTGCTGCAGTCTCCGAGAGAGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGaa                <  1:388201/146‑1 (MQ=255)
                                                                                                                                      aGCCTGCTAGCTGTCGGGGTGAATTTGCTTGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACt              <  1:1212693/149‑1 (MQ=255)
                                                                                                                                         cTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGGCATTGACGTAGACGTCGAACTGcc           <  2:591501/149‑1 (MQ=255)
                                                                                                                                           gCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCg         >  1:745355/1‑149 (MQ=255)
                                                                                                                                           gCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCg         >  1:1108144/1‑149 (MQ=255)
                                                                                                                                            cTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCgg        >  2:684593/1‑149 (MQ=255)
                                                                                                                                            cTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCgg        >  2:894157/1‑149 (MQ=255)
                                                                                                                                               gCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTg                              >  2:179644/1‑124 (MQ=255)
                                                                                                                                               gCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTg                              <  1:179644/124‑1 (MQ=255)
                                                                                                                                                 tGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCa   <  2:231888/149‑1 (MQ=255)
                                                                                                                                                  gTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCag  >  2:1556866/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGCCTCAG  >  NC_000913/265441‑265735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑