Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,634 C→T 100% E38K (GAG→AAG)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,6340CT100.0% 309.8 / ‑6.7 104E38K (GAG→AAG) yjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (51/51);  minor base G (1/1);  total (52/52)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 104 total aligned reads displayed.

ACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGC  >  NC_000913/4633488‑4633778
                                                                                                                                                  |                                                                                                                                                
aCTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                <  2:702727/149‑1 (MQ=255)
aCTGAAAGTGCTGTCACCATCGATGCCACCTTCAATCGCCACCCAAAAATCAGCCGCTGGAAGTATACGGCGGGCATTGGCTACCCGATTTCGTGAGCCAGCGCGCGTTTCCTCACTGCCAACGGGCTGTTCCGGTACACCGCTCTTGa                                                                                                                                                <  2:1019772/149‑1 (MQ=255)
 atGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGAc                                                                                                                                               <  2:875267/148‑1 (MQ=255)
  tGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACg                                                                                                                                              <  2:875927/149‑1 (MQ=255)
   gAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCGCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACgg                                                                                                                                             <  1:892695/149‑1 (MQ=255)
    aaaGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAACATCAGCCTCTGGTAGTAACCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGATGCGTCACTGCCAAAGGGCTGTTCCGGTAGCCCGATCTTGACGGc                                                                                                                                            >  2:1318002/1‑149 (MQ=255)
      ccgcccgTCACCATCCATTCCATCTTCCCCCGCCACGCAATAATCATCCTCTGGACGTATACGGCGGGCATTTGCTACCCGAATTCGTGCGCTTGCGCGCGTTTCCTCACTGCCAAAGCGCTGTTCCGTTACACCGCTCTTGACGGCat                                                                                                                                          <  2:1084644/143‑2 (MQ=255)
      aGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGAAAACGGCGGGCATTGGCTACCCGCTTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCac                                                                                                                                          >  2:922667/1‑148 (MQ=255)
           tGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                     >  1:798126/1‑149 (MQ=255)
           tGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                     >  2:588239/1‑149 (MQ=255)
           tGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAt                                                                                                                                     >  2:24168/1‑149 (MQ=255)
             tCACAATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGc                                                                                                                                   >  2:1388956/1‑149 (MQ=255)
             gcACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGc                                                                                                                                   <  2:1415141/148‑1 (MQ=255)
              caacATCCATGCCAGCTTAAATCGCCACCAAAAAATCAGCCTCTTGAAGTAAACGGCGGGCATTGTCTACACGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCTGTACACCGCTCTTGACGGCAACGGATGCa                                                                                                                                  <  2:1510838/146‑1 (MQ=255)
                 cATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCCCCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCAGGTACACCGCTCTTGACGGCAACGGATGCAata                                                                                                                               >  1:284861/1‑149 (MQ=255)
                 cATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAata                                                                                                                               <  2:578772/149‑1 (MQ=255)
                  aTCGATGCCAGCTTCCATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTCCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAatat                                                                                                                              >  2:1106350/1‑149 (MQ=255)
                     gATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGc                                                                                                                           <  2:458050/149‑1 (MQ=255)
                      ttGCAAGCTTAAATCGCCACCGAAATATCAGGCTCTGGAAGTTCACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAATGGCTGTTCCGGTACACCTCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                          <  1:855197/148‑1 (MQ=255)
                      ctGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                          <  1:401549/148‑1 (MQ=255)
                      aTGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCa                                                                                                                          >  1:1360361/1‑149 (MQ=255)
                           aGCGTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATc                                                                                                                     <  1:1204905/149‑1 (MQ=255)
                              ttCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTGCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCtt                                                                                                                  <  2:1433252/149‑1 (MQ=255)
                                 aaTCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                               <  1:237037/149‑1 (MQ=255)
                                 aaTCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGc                                                                                                               >  2:803220/1‑149 (MQ=255)
                                 aaTCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCGTCGc                                                                                                               >  2:809883/1‑149 (MQ=255)
                                   tCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCg                                                                                                             >  1:462786/1‑149 (MQ=255)
                                     gCCCCCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCGACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATCTGGCAGGATCCTTCGCCGaa                                                                                                           >  1:1409671/1‑149 (MQ=255)
                                     gCCACCCAAAAATCAGCCTCTGGAAGTAACCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCATAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGaa                                                                                                           <  2:1214085/149‑1 (MQ=255)
                                        aCCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAt                                                                                                        >  2:743669/1‑149 (MQ=255)
                                         cccAAAAATCACCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtc                                                                                                       <  1:326934/149‑1 (MQ=255)
                                          ccAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtct                                                                                                      >  2:1557145/1‑149 (MQ=255)
                                           cAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                     >  2:609042/1‑149 (MQ=255)
                                           cAAAAAGCAGCCTCTGGAAGTAAACGGCGGTCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGAGGCAATATGGCAGGATCCTTCGCCGAAGAtctc                                                                                                     <  1:750601/149‑1 (MQ=255)
                                            aaaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCATTCGCCGAAGATCTCg                                                                                                    >  2:273639/1‑149 (MQ=255)
                                            aaaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGACAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTACGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCg                                                                                                    >  1:520675/1‑149 (MQ=255)
                                            aaaaaTCAGCCTCGGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTGCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGCCGGCACCGGCTGCAAGATGGCAGGATCCTTCGCCGCAGATcgcg                                                                                                    >  1:1305054/1‑149 (MQ=255)
                                             aaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                   <  1:91475/149‑1 (MQ=255)
                                             aaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                   <  2:1545326/149‑1 (MQ=255)
                                             aaaaTCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGt                                                                                                   <  2:917170/149‑1 (MQ=255)
                                               caTCAGCCGCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTATCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGa                                                                                                 <  2:512124/148‑1 (MQ=255)
                                                  cAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAAt                                                                                              <  1:921703/149‑1 (MQ=255)
                                                    gCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCATGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGc                                                                                            >  2:7551/1‑149 (MQ=255)
                                                     ccTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGcc                                                                                           >  1:1491968/1‑149 (MQ=255)
                                                       tctGGAAGTAATCGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACCCCGCTCTTGACGGCAACGGATGCAATTTGGCAGGATCCTTCGCCGAACATCTCGTGAAATGCCTg                                                                                         >  1:862290/1‑149 (MQ=255)
                                                       tctGGAAGTAAACGGCGGGCATTGGCTCCCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGAAGGATCCTTCGCCGAAGATCTCTTGAAATGCCTg                                                                                         >  1:940128/1‑149 (MQ=255)
                                                       tctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTg                                                                                         <  2:521590/149‑1 (MQ=255)
                                                        ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                        >  1:884774/1‑149 (MQ=255)
                                                        ctGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCGCTTGACGGAAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGc                                                                                        <  2:171579/149‑1 (MQ=255)
                                                         tGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCa                                                                                       <  1:948285/149‑1 (MQ=255)
                                                           gAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGa                                                                                     >  2:619282/1‑149 (MQ=255)
                                                                aaaCGGCGGGCATTGGCTACCCGATTTCGGGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGc                                                                                <  1:353895/149‑1 (MQ=255)
                                                                     gcggGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGaa                                                                           <  1:1554968/149‑1 (MQ=255)
                                                                     gcggGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGaa                                                                           <  1:363872/149‑1 (MQ=255)
                                                                     gcggGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGaa                                                                           >  1:468397/1‑149 (MQ=255)
                                                                       gggCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCGTCACTGACAAAGGGCTGTTCCGGTACACCTCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGGCTGCAGAATGGCCTGAAtt                                                                         <  1:1416475/149‑1 (MQ=255)
                                                                          cATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGCATTTTa                                                                      >  2:56294/1‑149 (MQ=255)
                                                                          cATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTa                                                                      <  1:24168/149‑1 (MQ=255)
                                                                             tGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCGGGATCATTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCg                                                                   <  1:92677/149‑1 (MQ=255)
                                                                              ggCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCgg                                                                  >  1:45585/1‑149 (MQ=255)
                                                                                    ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCATAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGCTGCAATATGGCAGGATCCTTCGCCGAAGCTCTCGTGAAAAGCCTGCAGAATGGCCTGAATTTTAGCGGGATggg                                                            >  1:1501393/1‑149 (MQ=255)
                                                                                    ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                            <  1:745586/149‑1 (MQ=255)
                                                                                    ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtgg                                                            <  1:855904/149‑1 (MQ=255)
                                                                                    ccGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGATCTTGCCGGCCACGGATGGATTATGGCAGGCTCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGCATGGCCTGAATTTTAGCGGGTTtgg                                                            >  1:321662/1‑149 (MQ=255)
                                                                                     cGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATtggt                                                           >  1:216309/1‑149 (MQ=255)
                                                                                         ttCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTc                                                       <  1:1388956/149‑1 (MQ=255)
                                                                                            gTGCGCCAGCGCGCGTTTCCTCACTGCCACAGGGCTGTTCCGGTTCACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGATTTTTAGCGGGATTGGTGGTCGca                                                    <  2:515507/149‑1 (MQ=255)
                                                                                            gTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGca                                                    >  1:1341314/1‑149 (MQ=255)
                                                                                            gTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGca                                                    >  1:189939/1‑149 (MQ=255)
                                                                                              gcgcCAGCGCGCGTTTCCTCACAGCCAAACGGCGGTTCCGGTACACCGCTCTTGACGGCACCGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGcaca                                                  <  2:232006/149‑1 (MQ=255)
                                                                                                gcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                <  2:798126/149‑1 (MQ=255)
                                                                                                gcCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGa                                                <  1:922667/149‑1 (MQ=255)
                                                                                                 ccAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTGCGGTACACCGCGCTTGACGGCAACGGATGGAATATGGGAGGATACGTCGCCGCAGATCTCGTGAAATGCCGGCAGAATGGCCTGAATTTTAGCGGGTTTGGGGGTCGCACAGAc                                               >  2:257503/1‑149 (MQ=255)
                                                                                                        gcgTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCTGGATCCTTCGCCGAAGATCTCGTGAAATGCCAGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTgg                                        >  2:452241/1‑149 (MQ=255)
                                                                                                          gTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCACCGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGTATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTg                                      <  1:149043/149‑1 (MQ=255)
                                                                                                           tttCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGc                                     <  1:609042/149‑1 (MQ=255)
                                                                                                             tCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCAt                                   >  1:16784/1‑149 (MQ=255)
                                                                                                                tCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAAt                                >  1:545334/1‑149 (MQ=255)
                                                                                                                    tgcacacgggATGTTCCGGTACACCGCTCTTGCCGGCAACGGATGCAATATGGCCGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGCATGGCCGGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGc                            <  2:1305054/142‑1 (MQ=255)
                                                                                                                    tGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGc                            <  1:588239/149‑1 (MQ=255)
                                                                                                                    tGCCAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGc                            >  1:917228/1‑149 (MQ=255)
                                                                                                                      ccAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAt                          >  2:1152021/1‑149 (MQ=255)
                                                                                                                      ccAAAGGGCTGTTCCGGTACACCGCTCTGGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAt                          >  1:185700/1‑149 (MQ=255)
                                                                                                                       cAAAGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCAtt                         >  2:496674/1‑149 (MQ=255)
                                                                                                                        aaaGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAAGATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGATTTGCCTGAAGTTTCGCGGGATTGTTGGTCGCACTGAGAACTTGTTGCATACTCAGCATTa                        >  2:880898/1‑149 (MQ=255)
                                                                                                                         aaGGGCTGTTCCGGTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTAc                       <  2:884774/149‑1 (MQ=255)
                                                                                                                                   ccGGTACACCGCTCTTGACGGCAACGGATGCAATATGCCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGGCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAtt             <  2:1461005/149‑1 (MQ=255)
                                                                                                                                     ggTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTaa           >  1:873144/1‑149 (MQ=255)
                                                                                                                                      gTACACCGCTCTTGACGGCAACGTATGCAATATGGCAGTTTCCTTCGCCGAAGATCTCGTGAAATTCCTGAAGTATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACATCTTGGTGCTTAATCAGCATTACTCAGAAAATTAAc          <  2:862290/149‑1 (MQ=255)
                                                                                                                                      gTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAAc          <  2:462786/149‑1 (MQ=255)
                                                                                                                                      gTACACCGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTAGCCGAAGATCTCGAGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTGGGTGCATAATCAGAATTACTCAGAAAATTaaa          >  2:1316099/1‑148 (MQ=255)
                                                                                                                                           ccGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAc     >  1:610256/1‑149 (MQ=255)
                                                                                                                                           ccGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAc     <  2:1360361/149‑1 (MQ=255)
                                                                                                                                           ccGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAc     <  1:1557145/149‑1 (MQ=255)
                                                                                                                                            cGCTCTTGACGTCAACGGAAGCAATATGGCAGGATCCTTCGCAGAAGATATCGTGAAATGCCTGCAGAATGGCCTGAATTATAGCGGGATTGGTGGACGCACAGACAACTTGGTGCTTAAAAAGCATTACTCAGAAAATTAACGTTAca    >  2:1087553/1‑149 (MQ=255)
                                                                                                                                            cGCTCTTGACGGCAACGGATGCAATATGGCAGGTTCCTTCGCCGATGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCCCAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAca    >  1:226620/1‑149 (MQ=255)
                                                                                                                                            cGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTTGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAATTTAACGTTAca    <  1:10461/149‑1 (MQ=255)
                                                                                                                                            cGCTCTTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGAGTGGTGGTCGCACAGACAACGTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcc    >  2:30339/1‑148 (MQ=255)
                                                                                                                                              ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTAcagc  >  2:165496/1‑149 (MQ=255)
                                                                                                                                              ctctTGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGAGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAAATAACGTAAcacc  >  2:1339092/1‑147 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ACTGAAAGTGCTGTCACCATCGATGCCAGCTTCAATCGCCACCCAAAAATCAGCCTCTGGAAGTAAACGGCGGGCATTGGCTACCCGATTTCGTGCGCCAGCGCGCGTTTCCTCACTGCCAAAGGGCTGTTCCGGTACACCGCTCTCGACGGCAACGGATGCAATATGGCAGGATCCTTCGCCGAAGATCTCGTGAAATGCCTGCAGAATGGCCTGAATTTTAGCGGGATTGGTGGTCGCACAGACAACTTGGTGCATAATCAGCATTACTCAGAAAATTAACGTTACAGC  >  NC_000913/4633488‑4633778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: