Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 290,247 A→G 6.0% F20S (TTC→TCC)  argF ← ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913290,2470AG 6.0% 181.0 / 2.8 67F20S (TTC→TCC) argFornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base A (31/31);  new base G (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

GTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTC  >  NC_000913/290099‑290392
                                                                                                                                                    |                                                                                                                                                  
gTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCTGCAAGGGTCAGCAGAGAATTGa                                                                                                                                                    >  1:1047947/1‑149 (MQ=255)
   aaaTGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACt                                                                                                                                                 <  2:941326/149‑1 (MQ=255)
    aaTGCCGCACCTTCGCAAGCGCAACGGGTACGAGGAGAGTCTTTTTCTAATATGAGCGCGATGTTTTTACCGGTAAGCTTCTTTACTTTCTTGATATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACtg                                                                                                                                                <  2:1016838/149‑1 (MQ=255)
     aTGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACtgt                                                                                                                                               >  2:1095257/1‑149 (MQ=255)
       gCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGc                                                                                                                                             <  1:149109/149‑1 (MQ=255)
           cAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAggg                                                                                                                                         <  2:407374/149‑1 (MQ=255)
            aaCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAgggg                                                                                                                                        >  1:676962/1‑149 (MQ=255)
               ttCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGCTaa                                                                                                                                     >  1:879982/1‑149 (MQ=255)
                tCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTaaa                                                                                                                                    <  1:576416/149‑1 (MQ=255)
                  gAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGt                                                                                                                                  >  1:1554784/1‑149 (MQ=255)
                   aaaGCGCAACTGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTc                                                                                                                                 <  1:507196/149‑1 (MQ=255)
                      gagCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAATCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGGGAACTGTGCAGGGGTAAAGTCGAg                                                                                                                              <  1:912516/149‑1 (MQ=255)
                        gCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCa                                                                                                                            >  2:1265105/1‑149 (MQ=255)
                          aaCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGt                                                                                                                          >  1:1341186/1‑149 (MQ=255)
                            tggggACGAGTCGAGTCTTTTTCGCAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGttt                                                                                                                        <  1:945837/148‑1 (MQ=255)
                             gggTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTc                                                                                                                       >  2:1268599/1‑149 (MQ=255)
                                tACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGGAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTTAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGa                                                                                                                    <  1:1146813/149‑1 (MQ=255)
                                         agtcTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGtttt                                                                                                           >  1:443156/1‑149 (MQ=255)
                                             tttttCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGt                                                                                                       >  2:485813/1‑149 (MQ=255)
                                                   taaGATGAGAGCTATGTCTTTACCGGTAAGCTTCTGTACTTCCATGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAACTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTatatat                                                                                                 <  2:850339/148‑1 (MQ=255)
                                                        tGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCGGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCATTTTCAGAAAGTGTTTTTTGTTTAAATCGGAc                                                                                            >  2:867721/1‑149 (MQ=255)
                                                             gcgATGTTTTTACCGGTAAGCTTCTGTACTACATTGCCTTTTTTATTATCGGCTTTGAGCTGTGCGGCAATGGTCAGCAGAGAAGTGAACTCTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAtttt                                                                                       <  1:129243/149‑1 (MQ=255)
                                                              cgATGTTTTTACCGGTAAGCTGCTGTACTTCCTTGCCATTTTTTTAATCGGCTTTGAGCGGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTa                                                                                      <  1:1388803/149‑1 (MQ=255)
                                                                 tGTTTTTACCGGGAAGCTGCTGTACATCCTTGCCATTTTTTTTCTCGGCGTTGAGCTGGGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAACGTGTTTTTTGTATAAATCGGACATTTTATcc                                                                                   <  2:136375/149‑1 (MQ=255)
                                                                  gTTTTTACCGGTCAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCt                                                                                  <  2:223593/149‑1 (MQ=255)
                                                                  gTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTTCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACAGTTTATCCt                                                                                  >  2:1108199/1‑149 (MQ=255)
                                                                   tttttACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTatcatc                                                                                 >  2:311655/1‑149 (MQ=255)
                                                                   tttttACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGACGAGCAGTTTCAGAAATTGTTTTTTGTATAAATCGGACATTTTATCCTc                                                                                 >  2:362185/1‑149 (MQ=255)
                                                                         ccGGTAAGCTTCTGTACTTCCTTGCCA‑TTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGc                                                                          >  1:677179/1‑149 (MQ=255)
                                                                          gggTAAGCTTCTGTAGTTCCTTGCCATTTTTTTTTTCGGCTTTGAGAGGGGCGGCAAGGGTGAGCAGAGAAGTGAACTGTTCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGc                                                                          <  1:747659/148‑1 (MQ=255)
                                                                           ggTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCg                                                                         >  1:1285360/1‑149 (MQ=255)
                                                                            gTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGGGCTGTGCGGCAAGGGTCAGCAGAGAAGTGGACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGa                                                                        >  1:1465734/1‑149 (MQ=255)
                                                                            gTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGa                                                                        >  1:19362/1‑149 (MQ=255)
                                                                            gTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGATTTGGGATGTGCGGCAAGGGTCAGCAGAGAAGTGGACTGTGCAGGGGTAAAGTAGCGCAGTTTCATAAAGTGTTTTTTGTATAAATCGGAGCTTTTATCCTCGCAttgaga                                                                        >  1:1038458/1‑144 (MQ=255)
                                                                                gCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑c                                                                   <  1:526193/149‑1 (MQ=255)
                                                                                 cTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑cc                                                                  <  1:367272/149‑1 (MQ=255)
                                                                                 cTTCTGTACTTCCTTGCCATTTTTTTTATCGGATTTGAGATGTGCGGCAAGGGTCAGAAGAGATGTGAACTGTTCAGGGGTAAAGTCGAGAAGTTTCAGATATTGTTTTTTGTATAAATCGGACATTTTATCCTCGAATTGAGCACG‑cc                                                                  >  2:842683/1‑149 (MQ=255)
                                                                                   tCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCAc                                                                <  2:62505/149‑1 (MQ=255)
                                                                                   tCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTCTGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCAc                                                                <  2:822337/149‑1 (MQ=255)
                                                                                    cTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACt                                                               >  2:372526/1‑149 (MQ=255)
                                                                                    cTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACt                                                               >  2:1552022/1‑149 (MQ=255)
                                                                                      gTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGTACATTTTATCCTCGCATGGCGCACG‑CCACTTa                                                             >  1:421241/1‑149 (MQ=255)
                                                                                      gTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGAAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACT‑CCACTTa                                                             >  1:1181722/1‑149 (MQ=255)
                                                                                       tACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCTCATGGCGAACG‑CCACTTAt                                                            >  1:536478/1‑149 (MQ=255)
                                                                                       tACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTAt                                                            >  1:444898/1‑149 (MQ=255)
                                                                                       tACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTAt                                                            <  1:20568/149‑1 (MQ=255)
                                                                                        aCTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTAtt                                                           <  1:1423784/149‑1 (MQ=255)
                                                                                             cTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAAtt                                                      >  1:92878/1‑149 (MQ=255)
                                                                                              ttGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTa                                                     <  1:1095257/149‑1 (MQ=255)
                                                                                                  cATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAAt                                                 >  1:426278/1‑149 (MQ=255)
                                                                                                   aTTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAAtt                                                >  1:445545/1‑149 (MQ=255)
                                                                                                             tCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTatat                                      <  2:444898/149‑1 (MQ=255)
                                                                                                             tCGGCTTTGAGCTGGGCGGCAAGGGTCAGCAGAGAAGTGAAGTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTTTATAAATCGGACATTTTATCCTCGCATGGCGAACGACCAATT‑TTGAATTAAAATTCACTTTatat                                      >  2:857196/1‑149 (MQ=255)
                                                                                                               ggCTTTGAGCTGTTCGGCAAGGTTCAGCAGAGAAGTGAAATGTGCAGGGTTAAAGTCTATCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATAtgt                                    <  2:37636/149‑1 (MQ=255)
                                                                                                               ggCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATAtgt                                    <  2:1431121/149‑1 (MQ=255)
                                                                                                                  tttGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGCCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTa                                 >  1:491534/1‑149 (MQ=255)
                                                                                                                     gAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACT‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAatt                              >  1:1293076/1‑149 (MQ=255)
                                                                                                                        cTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAattatt                           <  1:1512516/149‑1 (MQ=255)
                                                                                                                           tgCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCAt                        <  2:676962/149‑1 (MQ=255)
                                                                                                                             cGGCAAGGGTAAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCAttt                      <  2:798860/149‑1 (MQ=255)
                                                                                                                                 aaGGGGCAGCAGAGAAGTTCACTGTGCAGGGGTACAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCaa                  <  2:1173321/149‑1 (MQ=255)
                                                                                                                                  aGGTTCATCAGAGAATTTGACTGTGCAGTTTTAAAGTAGAGCAGTTTCAGTAAGTTTTTTTTGTATAAATCTTACATTTTATCCTCGCATGGCGAACG‑CCAATTATTGAATTAAAATTCACTTTATATTTGTAATTATTCATTTGCAAc                 <  2:1038458/149‑1 (MQ=255)
                                                                                                                                  aGGGTCAGCAGAGAAGTGGACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAAc                 <  2:1465734/149‑1 (MQ=255)
                                                                                                                                   gggTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAAcc                >  1:1266268/1‑149 (MQ=255)
                                                                                                                                      tCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCa             <  2:1341186/149‑1 (MQ=255)
                                                                                                                                          cagAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAAATAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTc         <  1:1552022/149‑1 (MQ=255)
                                                                                                                                           agagAAGTGAACTGTGCAGGGGTAAACTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTca        <  2:879982/149‑1 (MQ=255)
                                                                                                                                                 gTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAAtta  >  2:1162370/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGCTTCTGTACTTCCTTGCCATTTTTTTTATCGGCTTTGAGCTGTGCGGCAAGGGTCAGCAGAGAAGTGAACTGTGCAGGGGTAAAGTCGAGCAGTTTCAGAAAGTGTTTTTTGTATAAATCGGACATTTTATCCTCGCATGGCGAACG‑CCACTTATTGAATTAAAATTCACTTTATATGTGTAATTATTCATTTGCAACCCCATTTCACAATTC  >  NC_000913/290099‑290392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑