Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 304,790 T→G 5.5% E139A (GAG→GCG)  ecpE ← ECP production pilus chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913304,7900TG 5.5% 309.6 / 4.0 117E139A (GAG→GCG) ecpEECP production pilus chaperone
Reads supporting (aligned to +/- strand):  ref base T (60/50);  new base G (5/2);  total (65/52)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAAC  >  NC_000913/304642‑304936
                                                                                                                                                    |                                                                                                                                                  
ttCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                                                                                                                                    >  1:532521/1‑149 (MQ=255)
ttCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACAGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCt                                                                                                                                                    >  1:384665/1‑149 (MQ=255)
 tCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTc                                                                                                                                                   <  1:678166/149‑1 (MQ=255)
     tCGAATCCCATCCTGGCTTAATCAGTAGCTTAAACCATGTGATGCCAGTGTAACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAAGGGTATCCATCACCACCACCGGCTCCGTg                                                                                                                                               <  1:1520844/149‑1 (MQ=255)
     tCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGCCGGGGTCGTCCTACCAGAATGGTAGCCATCACCACCACCGGCTCCGTg                                                                                                                                               >  2:896520/1‑149 (MQ=255)
       gAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCt                                                                                                                                             >  1:781272/1‑149 (MQ=255)
        aaTCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTg                                                                                                                                            >  2:1178433/1‑149 (MQ=255)
        aaTCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTg                                                                                                                                            >  2:1547849/1‑149 (MQ=255)
         aTCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCCCCACCACCGGCTCCGTGCttc                                                                                                                                           >  2:1512600/1‑147 (MQ=255)
          tCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTAGTTACCGTTCCTGTCCCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                                                                                                                          >  2:1200030/1‑149 (MQ=255)
          tCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGAc                                                                                                                                          >  2:505097/1‑149 (MQ=255)
             caTCCTGGCTTAATCTGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCTCCGGCTCCGTGCTGACCAc                                                                                                                                       <  2:762655/149‑1 (MQ=255)
             caTCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCAc                                                                                                                                       >  2:266058/1‑149 (MQ=255)
                    gCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTTAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGTCTCCGTGCTGACCACGccgccg                                                                                                                                >  1:980873/1‑149 (MQ=255)
                    gCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGccgccg                                                                                                                                >  2:562036/1‑149 (MQ=255)
                         aTCAGTAGCTTAAACCAAGTGTTGCCAGTGTAACTTAACGTTCCTCTCACCTTGTCGAAGGACCATTTAAACTGAACCTGCCGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTgg                                                                                                                           <  2:877639/149‑1 (MQ=255)
                           cAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATTGTATCCATCACCACCACCGGCGGCGTGGTCAGCACGGAGCCGGtggtgg                                                                                                                         <  2:293110/149‑6 (MQ=255)
                              tAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc                                                                                                                      <  2:653216/149‑1 (MQ=255)
                              tAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGGTGGGCTGc                                                                                                                      >  1:1041378/1‑149 (MQ=255)
                              tAGCTTAAACCATGAGTTGCCCGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGc                                                                                                                      <  1:1326093/149‑1 (MQ=255)
                                gCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGGCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGt                                                                                                                    <  1:698777/149‑1 (MQ=255)
                                 cTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTc                                                                                                                   >  2:408818/1‑149 (MQ=255)
                                  ttAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                                                                                                  >  1:592320/1‑149 (MQ=255)
                                  ttAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCt                                                                                                                  >  1:527139/1‑149 (MQ=255)
                                    aaaaCATGTGTTGCCAGTGTAACTTACCGTTCCTGTCACCATCTCGATGGACCATTTAAACGGACCCTCACGTCGTCGGACTACCAGCATGGTATCCATCACCACCCCCCGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTg                                                                                                                <  2:702177/149‑1 (MQ=255)
                                      aCCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGCCCACGCCGCCGGTTGGGCTGCGTCTTGtt                                                                                                              >  2:1561635/1‑148 (MQ=255)
                                       ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                                                                                             >  2:397958/1‑149 (MQ=255)
                                       ccATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAt                                                                                                             >  2:688977/1‑149 (MQ=255)
                                        cATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATg                                                                                                            >  2:505044/1‑149 (MQ=255)
                                         aTGTGTTGCCAGTGATACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATgg                                                                                                           <  1:902089/149‑1 (MQ=255)
                                             gtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGACTGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTAc                                                                                                       <  2:437328/149‑1 (MQ=255)
                                             gtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTAc                                                                                                       <  1:676280/149‑1 (MQ=255)
                                             gtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACg                                                                                                      >  1:915374/1‑149 (MQ=255)
                                             gtTGCCAGTGTTACTTACCGTTCCTGTCACCTTGGCGGAGGACCATTTAACCTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTAc                                                                                                       <  1:1029002/149‑1 (MQ=255)
                                             gtTGCCAGTGTTACTTACCGTTCCTGTCAACTTGTCGAAGGACCATTTAAACTGAACCTGCCGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGCCCACGCCTCCGGTTTGGCTGCGTCTTGTATGGTGAc                                                                                                       <  2:1394523/149‑1 (MQ=255)
                                               tGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGa                                                                                                     <  1:498652/149‑1 (MQ=255)
                                                gacAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCAACGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTCTAAGGTTACGAg                                                                                                    >  1:1079822/3‑149 (MQ=255)
                                                   aGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTgg                                                                                                 <  2:12928/149‑1 (MQ=255)
                                                       ttacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGAc                                                                                             <  2:888157/149‑1 (MQ=255)
                                                       ttacttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTAAGAGTGGGGAc                                                                                             >  2:532179/1‑149 (MQ=255)
                                                         acttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCTGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCt                                                                                           >  1:1259393/1‑149 (MQ=255)
                                                          cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCCTCCCCACCACCGGCGCCGTGCGGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGCCCTc                                                                                          >  1:144295/1‑149 (MQ=255)
                                                          cttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                                                                                          >  1:720777/1‑149 (MQ=255)
                                                          cttacCGTTCCTGTCACCTTGTCGAAGGACAATTTAAACTGAACCTGACGTGGTCGTTCTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTc                                                                                          >  2:240011/1‑149 (MQ=255)
                                                           ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCTCCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcg                                                                                         <  2:1233899/149‑1 (MQ=255)
                                                           ttacCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCAGGTTCGGCTGCGTCATGTATGGTTACGAGTCGGGACCAcg                                                                                         >  2:154913/1‑149 (MQ=255)
                                                             acCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTcgcg                                                                                       >  2:1790/1‑149 (MQ=255)
                                                              ccGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAGGGTTACGAGTGGGCACCTCGCGa                                                                                      >  1:193736/1‑149 (MQ=255)
                                                               cGTTCCTGTCCCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGaa                                                                                     <  1:381216/149‑1 (MQ=255)
                                                                 ttCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAAt                                                                                   <  1:941539/149‑1 (MQ=255)
                                                                 taccTTTCACCTTTTAGATTGATCAGTTAAACTGAACGTGTTGTGGTTGTAGTACTATAATGGTATCTATAAACACTACCGGATCAGTGCTGACCACGCCGCCTGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAAt                                                                                   <  2:1079822/147‑1 (MQ=255)
                                                                         aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCg                                                                           >  2:187615/1‑149 (MQ=255)
                                                                         aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCg                                                                           >  2:1472413/1‑149 (MQ=255)
                                                                         aCCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCg                                                                           >  1:717322/1‑149 (MQ=255)
                                                                           cTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCACGCGAAATGAGACCCGgt                                                                         >  2:6024/1‑149 (MQ=255)
                                                                            ttGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGgta                                                                        >  1:1517308/1‑149 (MQ=255)
                                                                             tGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGgtag                                                                       <  1:882418/149‑1 (MQ=255)
                                                                              gTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGATGGGCTGCGTGTTGTATGGTTACGAGTGGGGAACTCGCGCAATGCGACCCGgaagt                                                                      >  2:1414498/1‑149 (MQ=255)
                                                                              gTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCACAATGTTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCCGTTGGGCTGCGTCTAGTTTGGTTACGAGGGGGGCCCTCGCGAATTGAGACCCGgtaga                                                                      >  1:839460/1‑148 (MQ=255)
                                                                                  aaGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAgcg                                                                  >  1:1266036/1‑149 (MQ=255)
                                                                                     gACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTAGGGGTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTc                                                               >  1:1559755/1‑149 (MQ=255)
                                                                                        cATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTcgcg                                                            <  1:201384/149‑1 (MQ=255)
                                                                                             aaaCTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTAt                                                       >  2:45801/1‑149 (MQ=255)
                                                                                                     ccTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGa                                               <  1:1178433/149‑1 (MQ=255)
                                                                                                      cTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGTCCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGAc                                              >  2:670052/1‑149 (MQ=255)
                                                                                                         aCGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAt                                           <  1:505097/149‑1 (MQ=255)
                                                                                                         aCGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGGGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGATCAt                                           <  1:516909/149‑1 (MQ=255)
                                                                                                           gTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGa                                         <  1:688977/149‑1 (MQ=255)
                                                                                                           gTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCGCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGa                                         <  1:244432/149‑1 (MQ=255)
                                                                                                            tGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGAt                                        <  1:1547849/149‑1 (MQ=255)
                                                                                                              gTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAg                                      <  1:463185/149‑1 (MQ=255)
                                                                                                                     tacCAGAATGGTATCCATCACCCCCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGGCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGCCCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCAGGAGAGTAAAAtt                               >  2:866638/1‑149 (MQ=255)
                                                                                                                     tacCAGAATGGTATCCATCACCACCACCGGCTCCGTGCGGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGGAACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAAtt                               <  1:101008/149‑1 (MQ=255)
                                                                                                                      acCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTAGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGAAGCGCTCGCGGTTATACCGTGGTCCATGATAGTAAAAttt                              >  1:415613/1‑149 (MQ=255)
                                                                                                                       ccAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTa                             <  2:505848/149‑1 (MQ=255)
                                                                                                                       ccAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTa                             >  2:176835/1‑149 (MQ=255)
                                                                                                                             tGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATAc                       >  2:56757/1‑149 (MQ=255)
                                                                                                                             tGGTATCCATCACCACCACCGGCTACGTGCTGACCACGCCGCCGGTTGGGATGCGTCTTGTATGGTTTCGAGTGGGGACCTAGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTAGCCCCTGGACCAGTCTAGGAAAATTTAAAAAAc                       >  2:871201/1‑149 (MQ=255)
                                                                                                                              ggTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGGGGACCATGATAGTAAAATTTAAAATACt                      >  2:1072803/1‑149 (MQ=255)
                                                                                                                                 ttCCATCACCACCACCGGCTCCGTGCTGACCCCGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGc                   <  1:140896/148‑1 (MQ=255)
                                                                                                                                 aTCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGGTACGAGTGGGGACCTCGCGCTATGAGACCCGGTAGTAGCGCTCGCGGTTAGCCCGGGGACCATGATAGTAAAATTTAAAATACTCGc                   >  1:1505070/1‑149 (MQ=255)
                                                                                                                                  tCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGct                  <  2:382201/149‑1 (MQ=255)
                                                                                                                                    cATCACCACCACCGGCTCCGTTCTGACCACGCCTCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCTAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGctct                <  1:807347/149‑1 (MQ=255)
                                                                                                                                    cATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGctct                <  1:814050/149‑1 (MQ=255)
                                                                                                                                     aTCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCtctc               >  1:794361/1‑149 (MQ=255)
                                                                                                                                     aTCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTGGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGGAGTAGCGCTCGCGGTTATCCCGTGGCCCATGATAGTAAAAGTTAAAATACTCGCtctc               >  2:1251837/1‑149 (MQ=255)
                                                                                                                                     aTCACCACCACCGGCTCAGTGCTGACCCCGCCGCAGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGGGGTTATACCGTGGAGCATTATAGTAAAATTTTAAATACTCGCtctc               >  2:1427398/1‑149 (MQ=255)
                                                                                                                                      tCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCtctcc              >  1:462919/1‑148 (MQ=255)
                                                                                                                                       caccaccacCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCAc             >  1:584022/1‑149 (MQ=255)
                                                                                                                                       caccaccacCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCAc             <  2:720777/149‑1 (MQ=255)
                                                                                                                                         ccaccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCa           <  1:562036/149‑1 (MQ=255)
                                                                                                                                          cccaaccGGCGGCGTGGTCAGCACGGAGCCGGTGCGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAAAGAGACCAGGTAGTAGCGCTCGCGGTTAACCCGTGGACCATGATAGTAAAATTTAACAAACTCGCTCTCACCAg          >  1:1101258/5‑149 (MQ=255)
                                                                                                                                          caccaccGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGCCCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAg          >  1:1281755/1‑149 (MQ=255)
                                                                                                                                           ccaaccGGCGGCGTGGTCAGCACGGAGCCGGTTGGGCGGCGTCTTGGATGGTTACGAGGGGGGACCTCGCGAAATGAGACGCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGAGAGTAAAATTTAAAATACTCGCTCTCACCAGc         >  1:1466058/4‑149 (MQ=255)
                                                                                                                                            caaccGGCGGCGTGGTCAGCACGGAGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc        >  2:740894/3‑149 (MQ=255)
                                                                                                                                            caaccGGCGGCGTGGTCAGCACGGAGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGcc        >  1:1089871/3‑149 (MQ=255)
                                                                                                                                              accGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTg      <  1:187615/149‑1 (MQ=255)
                                                                                                                                                cGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCa    <  1:79689/149‑1 (MQ=255)
                                                                                                                                                 ggCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCaa   >  2:1166717/1‑149 (MQ=255)
                                                                                                                                                  gCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCacc  >  1:1453743/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCGGTCGAATCACATCCTGGCTTAATCAGTAGCTTAAACCATGTGTTGCCAGTGTTACTTACCGTTCCTGTCACCTTGTCGAAGGACCATTTAAACTGAACCTGACGTGGTCGTACTACCAGAATGGTATCCATCACCACCACCGGCTCCGTGCTGACCACGCCGCCGGTTGGGCTGCGTCTTGTATGGTTACGAGTGGGGACCTCGCGAAATGAGACCCGGTAGTAGCGCTCGCGGTTATCCCGTGGACCATGATAGTAAAATTTAAAATACTCGCTCTCACCAGCCTGCAAC  >  NC_000913/304642‑304936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: