Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 329,493 G→A 5.1% D11N (GAC→AAC)  betT → choline transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913329,4930GA 5.1% 375.9 / 6.9 137D11N (GAC→AAC) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base G (66/62);  new base A (2/5);  total (69/68)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

CATTCATCAGCGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCT  >  NC_000913/329346‑329641
                                                                                                                                                   |                                                                                                                                                    
cATTCATCAGCGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGa                                                                                                                                                     <  1:1280293/149‑1 (MQ=255)
     atcaGCGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACaaaa                                                                                                                                                >  1:1355026/1‑149 (MQ=255)
        aGCGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACTGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAaatca                                                                                                                                             >  2:1038898/1‑149 (MQ=255)
         gctgtgtTTATATATTTAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTTATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTTGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAaatcaa                                                                                                                                            <  1:1136897/146‑1 (MQ=255)
           ggTGTTTATCAATAAAAGCTGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAaatcaatc                                                                                                                                          <  1:1088195/149‑1 (MQ=255)
             tgtTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACATTTCACACAGCAGGGAAAAGGACAAAATCAATCCg                                                                                                                                        >  1:305646/1‑149 (MQ=255)
              gtTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCgg                                                                                                                                       >  1:671282/1‑149 (MQ=255)
                 tATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCggtgg                                                                                                                                    >  2:222940/1‑149 (MQ=255)
                  aTCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCggtggt                                                                                                                                   <  2:267513/149‑1 (MQ=255)
                     tATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGtt                                                                                                                                >  1:1426338/1‑149 (MQ=255)
                      aTTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGttt                                                                                                                               >  1:1415126/1‑149 (MQ=255)
                      aTTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGAACTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGttt                                                                                                                               >  1:1388443/1‑149 (MQ=255)
                       ttAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACGTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGtttt                                                                                                                              <  1:144918/149‑1 (MQ=255)
                        ttaaGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTa                                                                                                                             <  1:1507153/147‑1 (MQ=255)
                          aaGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACATTAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTaca                                                                                                                           >  1:39485/1‑149 (MQ=255)
                            gCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACAcc                                                                                                                         >  2:817838/1‑149 (MQ=255)
                             cGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTATACACCt                                                                                                                        >  1:1036184/1‑149 (MQ=255)
                               gTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTcc                                                                                                                      >  2:1289638/1‑149 (MQ=255)
                                ttATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTccg                                                                                                                     >  2:1032599/1‑149 (MQ=255)
                                   ttgattgGACGTGCCATATATCAGGTGTCTTTATTGTTACGACTTTGATTGTAAATCGGACCACTAACAGTGTGGCTACTGGATGCCAGCCCTTTCCCACTGCAGGGACAAGGACAAAATCAATCCGGTGGGGTGTTACCCCTccgccg                                                                                                                  <  2:1417928/149‑1 (MQ=255)
                                     gattgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGa                                                                                                                >  1:1265026/1‑149 (MQ=255)
                                      attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGaa                                                                                                               >  1:887859/1‑148 (MQ=255)
                                      attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGAc                                                                                                               <  1:691947/149‑1 (MQ=255)
                                      attgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGAc                                                                                                               >  1:1476480/1‑149 (MQ=255)
                                       ttgGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGTGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCAGCCGGACt                                                                                                              >  2:1345855/1‑149 (MQ=255)
                                         ggATGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGa                                                                                                            >  2:7190/1‑149 (MQ=255)
                                          gACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGTAAAAGGCCAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                           >  2:1549542/1‑149 (MQ=255)
                                          gACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                           >  1:1336042/1‑149 (MQ=255)
                                          gACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                           <  1:429430/149‑1 (MQ=255)
                                          gACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAt                                                                                                           >  2:476036/1‑149 (MQ=255)
                                             gTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAtttt                                                                                                        >  1:949934/1‑149 (MQ=255)
                                             gTTCAATATAAAATGTGTCTTAATTGTTACGAAGTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAAACAATCCGGTGGTGTTTTACACCTCCGACGGACTCAtttt                                                                                                        >  2:856770/1‑149 (MQ=255)
                                               ccacaataAAATGTGTCTAAATTCTGACTAAGATGATTTTAAATAGTACCACTAACAGTGGTGATACAGGATGACACACCTTTCACACAGCAGGGAAAAGGACAAAATCACTCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgt                                                                                                      <  1:1571614/144‑1 (MQ=255)
                                                 aaTATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGTATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTttgttg                                                                                                    >  1:742309/1‑149 (MQ=255)
                                                   tataAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGAAACAATAACAGTGGGGATACTGGATGACAGACCTTT‑‑CACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTCCACCTCCGCAGGACTGATTTTGTTGtttt                                                                                                >  2:343838/1‑149 (MQ=255)
                                                    ataAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTAACACAGAAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGCACTGATTTTGTTGttt                                                                                                 >  1:717965/1‑149 (MQ=255)
                                                     aaacaTGTGGCTTAAATGTTACGCATTAGATATTAAATAGTAACAATAACAGTGGGGATACTGGCTGACAGACCATTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGGGTTTTACACCTCCGCCGGACTGATTTTGTTGtttt                                                                                                <  1:1452339/145‑1 (MQ=255)
                                                      aaaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACGAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTc                                                                                               <  2:1254989/149‑1 (MQ=255)
                                                      aaaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTc                                                                                               >  2:217967/1‑149 (MQ=255)
                                                       aaaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTcc                                                                                              <  1:500933/149‑1 (MQ=255)
                                                        aaTTTGTCTTAATTGTTTCGAATTTTATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTccc                                                                                             <  2:483101/149‑1 (MQ=17)
                                                        aaTGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTccc                                                                                             <  1:574767/149‑1 (MQ=255)
                                                         aTGTCTCTTAATTGTTACTAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCt                                                                                            <  1:753072/149‑1 (MQ=255)
                                                          tgtgTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTg                                                                                           <  1:1254509/149‑1 (MQ=18)
                                                           gtgtCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGa                                                                                          <  2:599533/149‑1 (MQ=255)
                                                             gtCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGCCCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGTACGGATTTTGTTGTTTTCCCTGACa                                                                                        >  1:310775/1‑149 (MQ=255)
                                                              tCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACaa                                                                                       <  2:638133/149‑1 (MQ=255)
                                                              tCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACaa                                                                                       <  1:1032599/149‑1 (MQ=255)
                                                              tCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTgctgt                                                                                       <  1:177682/149‑5 (MQ=17)
                                                              tCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTgctgt                                                                                       <  1:1248881/149‑5 (MQ=17)
                                                                ttAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACg                                                                                     >  1:1348306/1‑149 (MQ=255)
                                                                ttAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGAACAAAATCAATCAGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACg                                                                                     >  2:856762/1‑149 (MQ=255)
                                                                       ttACGATTTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGCAAACGATCCTGt                                                                              >  1:998479/1‑149 (MQ=255)
                                                                         aCGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATATTGTTTTTTTCCCTGACACCGATCCTGttt                                                                            >  2:1161375/1‑149 (MQ=255)
                                                                         aCGAATTTGATTTTAAATAGTAACAATAACAGTCGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGtta                                                                            >  2:688726/1‑148 (MQ=255)
                                                                          cGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTc                                                                           >  2:355984/1‑149 (MQ=255)
                                                                           gAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTcg                                                                          >  2:188696/1‑149 (MQ=255)
                                                                              tttGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGa                                                                       <  2:1256250/149‑1 (MQ=255)
                                                                                   ttttAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTtct                                                                  >  2:127176/1‑149 (MQ=255)
                                                                                     ttAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCg                                                                >  2:1461077/1‑149 (MQ=255)
                                                                                        aaTAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGTAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGcc                                                             <  1:266243/149‑1 (MQ=255)
                                                                                             tAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCTGGTAAAAGGACAAAATCAATCCGGTGGTGGTTTACACCTCCGCCGTACTGATTTTGTTTTTTTCCCTGACAACGTTCCTGTTTCGCGACTTCTCGGCCCtgtg                                                        <  1:42128/149‑1 (MQ=255)
                                                                                               aCAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGa                                                      >  1:277288/1‑149 (MQ=255)
                                                                                               aCAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCGGTGGa                                                      >  1:1191168/1‑149 (MQ=255)
                                                                                                cAATAACAGTGGGGATACGGGATGACCGACCTTGCACACCGCCGCTAAAATGACAAACTCAAACCGGGGGTTTTTTACACCTCCGCCGGAGTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAt                                                     <  2:91107/149‑1 (MQ=255)
                                                                                                 aaTAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGAtt                                                    <  1:290370/149‑1 (MQ=255)
                                                                                                   tAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTgg                                                  <  1:949489/149‑1 (MQ=255)
                                                                                                    aaCAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGc                                                 <  1:3797/149‑1 (MQ=255)
                                                                                                    aaCAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGc                                                 <  2:1470012/149‑1 (MQ=255)
                                                                                                     aCAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGGTTGGcc                                                <  2:1090531/149‑1 (MQ=255)
                                                                                                     aCAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGcc                                                <  2:949934/149‑1 (MQ=255)
                                                                                                         gggggATACTGGATGACAGCCCTATCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCAc                                            <  2:662512/148‑1 (MQ=255)
                                                                                                             gATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCTGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                        >  1:622896/1‑149 (MQ=255)
                                                                                                             gATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTg                                        <  2:1348306/149‑1 (MQ=255)
                                                                                                              aTACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTgg                                       >  2:408198/1‑149 (MQ=255)
                                                                                                              aTACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGACTGGCCGCACGCTgg                                       >  1:1271206/1‑149 (MQ=255)
                                                                                                                aCTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGAc                                     >  1:726380/1‑149 (MQ=255)
                                                                                                                   ggATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTgg                                  >  1:1396579/1‑149 (MQ=255)
                                                                                                                   ggATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGAATTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTgg                                  >  1:90806/1‑149 (MQ=255)
                                                                                                                       gACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGGGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGttt                              <  1:1470648/149‑1 (MQ=255)
                                                                                                                             ccTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTaaaa                        <  1:222940/149‑1 (MQ=255)
                                                                                                                              cTTTCACACAGCAGGGAAAAGGAGAAAATCAATCCGGGGGTGTTTTACACCTCCGCCTGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAAc                       <  1:856770/149‑1 (MQ=255)
                                                                                                                               tttCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAAcc                      >  1:1241463/1‑149 (MQ=255)
                                                                                                                                   acacaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCGCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCg                  <  2:1415126/149‑1 (MQ=255)
                                                                                                                                     acaGCCGGGAAAAGGCCAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                <  2:1533330/149‑1 (MQ=255)
                                                                                                                                     acaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGCTTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGCCTTCTCGGCCCTGTGGATTGGCCGCACGCTGGCCTGGGTTTCTAAAACCTTCGGt                >  1:915084/1‑149 (MQ=255)
                                                                                                                                     acaGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGt                >  2:958988/1‑149 (MQ=255)
                                                                                                                                        gcatGGAAAAGGAAAAAATAAATCCGGTGGTGTTTTACACCGCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTgg             <  1:1372042/149‑1 (MQ=255)
                                                                                                                                          agGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTa           <  1:110660/149‑1 (MQ=255)
                                                                                                                                          agGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTa           <  1:62777/149‑1 (MQ=255)
                                                                                                                                          agGGAAAACAACAAAATCAGTCCGGCGGAGGTGTAAAACACCACCGGATTGATTTTGTCCTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTa           >  1:665089/1‑149 (MQ=18)
                                                                                                                                           gggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTAc          <  1:224900/149‑1 (MQ=255)
                                                                                                                                            ggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACt         <  2:887859/149‑1 (MQ=255)
                                                                                                                                            ggAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACt         >  1:630984/1‑149 (MQ=255)
                                                                                                                                              aaaaGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTAt       >  1:260748/1‑149 (MQ=255)
                                                                                                                                              aaaaGGACAAAATCAATCCGGTGGTGGTTTACACCTCCGCCGGACTGATTTTGGTGTTTTCCCTGTCAACGATCCTGTTTCGCGTCTTCTCGGCCCTGTGGATTGGGCGCACGCTGGACTGGGTTTCTAAAACCTTCTGTTGGTACTAt       <  1:856762/149‑1 (MQ=255)
                                                                                                                                                aaGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCGCGGCCCTGTGGATTGGCCGCCCGCTGTACTGGGTTTCTAAACCCTGCGGTTGGTACTTTct     >  2:1405848/1‑149 (MQ=255)
                                                                                                                                                 aGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctg    <  2:305646/149‑1 (MQ=255)
                                                                                                                                                   gACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgct  >  2:1289100/1‑149 (MQ=255)
                                                                                                                                                   gACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATctgct  >  1:788565/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATTCATCAGCGGTGTTTATCTATTAAAGCGGTTATTGATTGGACGTTCAATATAAAATGTGTCTTAATTGTTACGAATTTGATTTTAAATAGTAACAATAACAGTGGGGATACTGGATGACAGACCTTTCACACAGCAGGGAAAAGGACAAAATCAATCCGGTGGTGTTTTACACCTCCGCCGGACTGATTTTGTTGTTTTCCCTGACAACGATCCTGTTTCGCGACTTCTCGGCCCTGTGGATTGGCCGCACGCTGGACTGGGTTTCTAAAACCTTCGGTTGGTACTATCTGCT  >  NC_000913/329346‑329641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: