Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 361,223 G→T 7.0% intergenic (+77/+26) cynX → / ← lacA putative cyanate transporter/thiogalactoside acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,2230GT 7.0% 220.6 / 4.4 96intergenic (+77/+26)cynX/lacAputative cyanate transporter/thiogalactoside acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (49/39);  new base T (2/5);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

GGGCTGATGATCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGA  >  NC_000913/361078‑361370
                                                                                                                                                 |                                                                                                                                                   
gggCTGATGATCATAACCCTGCGTTTTGCACCAGTACGTTTACCGCAGCTGTGGGTCAAAGATGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATACCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGTCCTGATAAGCGCAg                                                                                                                                                  <  2:272151/149‑1 (MQ=255)
     gatgatCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTAt                                                                                                                                             >  1:692426/1‑149 (MQ=255)
      atgatCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGCTGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTTTc                                                                                                                                            >  1:934827/1‑149 (MQ=255)
        gatCATAACCCTGCGTTTTGCACCAGTTCGTTTTCCGCAGCTGTGGGTCTAAGAGGCAGGAGGCGACGCTGGTTCCTGCGCGTTGTTCATGCCGGATGCGGCTAATGTAGCTCGCTGAACTTGTAGGCCTGATACGCGCAGCGTATCAg                                                                                                                                          >  1:910090/1‑149 (MQ=255)
        gatCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAg                                                                                                                                          >  2:365115/1‑149 (MQ=255)
         atCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAACGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATACGCTGCGCTTATCAgg                                                                                                                                         <  2:831120/149‑1 (MQ=255)
         atCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATACGCTGCGCTTATCAgg                                                                                                                                         <  1:130383/149‑1 (MQ=255)
         atCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATACGCTGCGCTTATCAgg                                                                                                                                         <  1:433310/149‑1 (MQ=255)
          tCATAACCCTGCGTTTTGCACCAGTACGTTTTCTGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATACGCTGCGCTTATCAGGc                                                                                                                                        <  2:1163015/149‑1 (MQ=255)
          tCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGc                                                                                                                                        >  1:548477/1‑149 (MQ=255)
             tAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGATAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGAAAt                                                                                                                                     >  1:707498/1‑149 (MQ=255)
               aCCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCCGCTAATGTAGATCGCTGAACTTCTAGGCCTGATAAGCGCAGCGTATCAGGCAAttt                                                                                                                                   <  2:324131/149‑1 (MQ=255)
                 ccTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAAttttt                                                                                                                                 >  2:1492342/1‑149 (MQ=255)
                      gTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTATTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAAt                                                                                                                            <  2:1191482/149‑1 (MQ=255)
                       ttttGCACCAGTACGTTTTCCGCCGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAAtt                                                                                                                           <  2:1429377/149‑1 (MQ=255)
                        tttGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAAttt                                                                                                                          >  1:65963/1‑149 (MQ=255)
                         tggCACCAGCACCGTTACCGGACCTGTGGGTCAAAGAGGCATGATGCGACGCTTCTTCCGGCGCTATGATCATGCCTGATGATGCTAAAGTAGTTCGCTGAAATTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAAttaa                                                                                                                         <  2:844781/147‑3 (MQ=255)
                         gtGCACCAGTACGTTTGCCGCAGCGGTGTGTCAAAGAGGCATGCTGCGACGCTTGTTCCTCCGCTTTGTTCATGCAGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTa                                                                                                                         <  1:853500/148‑1 (MQ=39)
                           gCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTaaa                                                                                                                       <  1:6472/149‑1 (MQ=255)
                           gCACCAGAACGATTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCCGCAAAAGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAAGCAATTTTAATAATTTaaa                                                                                                                       >  2:273773/1‑149 (MQ=255)
                            cACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAAc                                                                                                                      <  2:1433620/149‑1 (MQ=255)
                            cACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAAc                                                                                                                      >  2:68912/1‑149 (MQ=255)
                            cACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAAc                                                                                                                      >  2:58500/1‑149 (MQ=255)
                              ccAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTg                                                                                                                    >  1:318949/1‑149 (MQ=255)
                               cAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGa                                                                                                                   >  2:814862/1‑149 (MQ=255)
                                  tACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGa                                                                                                                >  1:1047225/1‑149 (MQ=255)
                                    cggtttCCGCGGCGGGGGGTCAAAGAGGCATGATGCGACGCGTGTTCCTGCGCTTGGGTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGAtt                                                                                                              <  2:268640/146‑1 (MQ=39)
                                      ttttCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCa                                                                                                            >  2:703025/1‑149 (MQ=255)
                                         tCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACt                                                                                                         >  2:1506514/1‑149 (MQ=255)
                                            gCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTta                                                                                                      >  2:1243345/1‑149 (MQ=255)
                                                 tgtgGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTAAACTTTATAATc                                                                                                 >  2:486496/1‑149 (MQ=255)
                                                  gtgGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCTCAGCGTATCAGGCAATATTTAGAATTTAAACTGACGATTCAACTTTATAATCt                                                                                                >  1:274807/1‑149 (MQ=255)
                                                   tgGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATTAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCtt                                                                                               <  2:412868/149‑1 (MQ=255)
                                                      gTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGa                                                                                            <  2:819893/149‑1 (MQ=255)
                                                       tCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGaa                                                                                           <  1:57475/149‑1 (MQ=255)
                                                         aaaGAGGCATGCTGCGACGCTGGGTCCTGCGCTTTGTGCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATCAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAaat                                                                                         <  2:863326/149‑1 (MQ=255)
                                                         aaaGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTATATAATTTAAACTGACGATTCAAATTTATAATCTTTGAaat                                                                                         >  1:20623/1‑149 (MQ=255)
                                                           agagGCTTGATGCGACGCTTGTTGCTGCGCTATGTTCATGCCGGATGCGGCTAATGTAGATAGCTGAACTTGTAGGCCTGATAAGCGCTGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAaataa                                                                                       <  2:1143214/149‑1 (MQ=255)
                                                           agagGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAaataa                                                                                       >  1:955627/1‑149 (MQ=255)
                                                           agagGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGAGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCCACTTTATTATCTTTGAaagaa                                                                                       >  1:1156889/1‑149 (MQ=255)
                                                             aggctggatgCGACGCTTGTTCCTGCGCTTTGATCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAataata                                                                                     <  1:1056064/143‑1 (MQ=255)
                                                             agGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAataata                                                                                     <  2:955627/149‑1 (MQ=255)
                                                               tcATTATGAGACGCTTGTTCCTTCGCTTTGTTCATGCCGGATTAGGATAATGTAGATCTCTTAACTTGTAGGCATTATAAGAGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGt                                                                                   <  2:1159694/148‑1 (MQ=255)
                                                               gCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGt                                                                                   >  1:811233/1‑149 (MQ=255)
                                                                cATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCTGATGCTGCTAATGTAGATCGCTGAACTTGTAGTCCTGATAAGCTCAGAGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTg                                                                                  <  1:108864/149‑1 (MQ=255)
                                                                cATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTg                                                                                  <  2:925828/149‑1 (MQ=255)
                                                                 atgatgCGACGCTTGATCCTGCGATTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGAGCAGCGTATCAGGCAATTTTTATTATTTAAACTGACGATTCAACTTTATAATCATTGAAATAAAAGtta                                                                                 >  1:1057586/1‑147 (MQ=255)
                                                                  tgatgCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCt                                                                                >  1:1557301/1‑149 (MQ=255)
                                                                  tgatgCGACGCTTGTTCCAGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCt                                                                                <  1:846563/149‑1 (MQ=255)
                                                                   gatgCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCtt                                                                               >  2:232899/1‑149 (MQ=255)
                                                                   gatgCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCtt                                                                               <  1:1480587/149‑1 (MQ=255)
                                                                   gatgCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCtt                                                                               >  1:9170/1‑149 (MQ=255)
                                                                      gCGACGCTTGTTCCTGCGCGTTGTTCATGCCGGATGCGGCTAATGTAGCTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCTTTTTTTATAATTTAAACTGCCGATTCCACTTTATAATAGTTGAAATTATAGTGCTTATc                                                                            >  1:1072458/1‑149 (MQ=255)
                                                                        gACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGACTGATACGCGCAGCGTATCAGGCAATTTTTATAATTTAAAATGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATccc                                                                          >  1:971391/1‑149 (MQ=255)
                                                                         aCGGTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGATCTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCg                                                                         <  2:1518907/149‑1 (MQ=255)
                                                                         aCGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCg                                                                         >  1:1280581/1‑149 (MQ=255)
                                                                          cGCTTGTTCCTGCGCTTTGTTCCTGCCGGTTGCGGCTAATGTAGCTCGCTGAACTTGGAGGCCTGATATGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTTACGAATCAACTTTATAATCTTTGAAATAAAAGTGCTTCTCCCgg                                                                        <  2:179873/149‑1 (MQ=255)
                                                                           gCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGt                                                                       >  2:354094/1‑149 (MQ=255)
                                                                            cTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTTATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTc                                                                      <  1:535082/149‑1 (MQ=255)
                                                                            cTTGTTCCTGCGCTTTGTTCATGACGGATGCGGCTAATCTAGATCGCTGAACTTGTAGGCCTTATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATATTAGTGCTTATCCCGGTc                                                                      <  2:915021/149‑1 (MQ=255)
                                                                                  ccTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTAt                                                                <  1:1167946/149‑1 (MQ=255)
                                                                                   cTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGAAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGATTATCCCGGTCGTTTAtt                                                               >  1:690298/1‑149 (MQ=255)
                                                                                    tGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTAttt                                                              >  1:757005/1‑149 (MQ=255)
                                                                                     gcgcTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCACGGTCGTTTATTTc                                                             >  2:1263175/1‑149 (MQ=255)
                                                                                      cgcgTTGTTCAGTCCGGATGCGGCTAATGTAGAGCGCTGAACATGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTTTAATCTTTGATATAATAGTGCTTATCCCGGTCGTTTATTTcg                                                            <  2:293595/149‑1 (MQ=255)
                                                                                       gcTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTcgc                                                           <  2:1122728/149‑1 (MQ=255)
                                                                                       gatttGATCATTCCGGATGCGTCTAATGTAGATCTCTGAACTTGTAGGCCTGATAATCGCAGCGTATCAGTCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTcgc                                                           <  1:779978/147‑1 (MQ=255)
                                                                                              tCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAc                                                    <  1:631883/149‑1 (MQ=255)
                                                                                              tCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGATTATCCCGGTCGTTTATTTCGCGAATAAc                                                    >  1:388269/1‑149 (MQ=255)
                                                                                                aTGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAccc                                                  <  2:318949/149‑1 (MQ=255)
                                                                                                 tGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCg                                                 >  1:232858/1‑149 (MQ=255)
                                                                                                  gCCGGATGCGGCTAATGTAGATCGCTGAACTTGTCGGCCTGATTAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATTGTGCTTCTCCCGGTCGTATATTTCGCGAATAACACgg                                                >  1:847218/1‑148 (MQ=255)
                                                                                                  gCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGa                                                <  2:757005/149‑1 (MQ=255)
                                                                                                   ccGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGAc                                               <  1:365115/149‑1 (MQ=255)
                                                                                                   ccGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGAc                                               <  1:65493/149‑1 (MQ=255)
                                                                                                    cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACACGaaa                                              >  2:1167562/1‑147 (MQ=255)
                                                                                                     ggATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACaa                                             <  2:1320779/149‑1 (MQ=255)
                                                                                                      ttctaggCTAATTTAGATCGCTGAACTTGGAGCCCGGATAAGCCCATCGTATAAGGCAATTTTAATAAATTAAACTGACGATTCAACTTTATAATCTTTGAAATAATATTGCTTCTCCCTGTCGTTTATTTCGCGAATAACCCGACAAg                                            <  2:1018954/144‑1 (MQ=255)
                                                                                                           ggCTAATGTAGATCGCTGAACTTGTAGTCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACg                                       >  2:937743/1‑149 (MQ=255)
                                                                                                           ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTCAACTGACGATTCAACTTTATAATCTTTCAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACg                                       <  2:724314/149‑1 (MQ=255)
                                                                                                           ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAAATGAAGATTCAACTTTATAATCTTTGAAATAATACTGCTTATCCCGGTCGTGTATTTCTCTCATAACCCGACAAGGagat                                       >  1:1018954/1‑146 (MQ=38)
                                                                                                             cTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGTATAACCCGACAAGGAACGcc                                     >  1:344615/1‑149 (MQ=255)
                                                                                                              tAATGTAGATCGTTGAAGTTGTAGGCCTGATAAGCGCAGAGTATCAGGAAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCa                                    <  2:1047225/149‑1 (MQ=255)
                                                                                                               aaTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATTATAGTACTTATCCCTGTCGTTTATTTCGCGTATAACCCGACAAGGAAAGCCAg                                   >  1:1085177/1‑149 (MQ=39)
                                                                                                                 tGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgcc                                 >  1:787473/1‑149 (MQ=255)
                                                                                                                  gTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgccg                                >  2:705674/1‑149 (MQ=255)
                                                                                                                     gATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCa                             <  2:548477/149‑1 (MQ=255)
                                                                                                                         gaagAACTTGTAGGCCTGATAAGCGGAGCGTATGAGGCAATTTTTATAATTTAAAGTGACGATTCAACTTTAGACTCTTTGAAAATATAGTGCTTAACCCGGTCTGTTATGTCGCGAATAACCCGACAAGGAACGCCACCCGCCccgac                         >  2:976271/4‑149 (MQ=255)
                                                                                                                         gCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCacgac                         >  2:6536/1‑149 (MQ=255)
                                                                                                                          cTGAACTTGAAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGAATATTTCGCGAATAACCCGACAAGTAACGCCATCCGCCacgacg                        >  1:1188083/1‑149 (MQ=255)
                                                                                                                               cTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtgg                   <  2:934827/149‑1 (MQ=255)
                                                                                                                                    aGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGaa              <  2:1347596/149‑1 (MQ=255)
                                                                                                                                     ggCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAACCCGACAAGGCACGCCAGCCGCCACGACGTTTGGTGGAAt             >  2:1174726/1‑149 (MQ=255)
                                                                                                                                      gCCTGATAAGAGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATACCCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATg            >  2:981093/1‑149 (MQ=255)
                                                                                                                                       ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGt           >  1:161387/1‑149 (MQ=255)
                                                                                                                                           aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCAGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCttt       >  2:710753/1‑149 (MQ=255)
                                                                                                                                               gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTtgtg   <  2:505850/149‑1 (MQ=255)
                                                                                                                                                cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGa  <  2:687448/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGGCTGATGATCATAACCCTGCGTTTTGCACCAGTACGTTTTCCGCAGCTGTGGGTCAAAGAGGCATGATGCGACGCTTGTTCCTGCGCTTTGTTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGA  >  NC_000913/361078‑361370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: