Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 558,385 T→G 6.9% R58R (CGT→CGG sfmA → FimA homolog, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913558,3850TG 6.9% 257.3 / 9.0 102R58R (CGT→CGGsfmAFimA homolog, function unknown
Reads supporting (aligned to +/- strand):  ref base T (46/49);  new base G (5/2);  total (51/51)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 104 total aligned reads displayed.

CGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTG  >  NC_000913/558237‑558533
                                                                                                                                                    |                                                                                                                                                    
cgcTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGt                                                                                                                                                      >  2:750591/1‑149 (MQ=255)
 gctggctgCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTa                                                                                                                                                     <  1:1077415/149‑1 (MQ=255)
  ctggctgCCGCACTATTCTCCGCTACGTGTAGTAATGCTGCCGTTGTAGATGGATGTACAATTCACTTAGAAGGCGAAATGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTAc                                                                                                                                                    <  2:935969/149‑1 (MQ=255)
     gctgCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGa                                                                                                                                                 <  1:1560948/149‑1 (MQ=255)
         ccGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGCTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAAAATCTTAACGAAAtt                                                                                                                                             >  2:254815/1‑149 (MQ=255)
           ccACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttga                                                                                                                                           <  1:1333839/148‑3 (MQ=255)
            cACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttgac                                                                                                                                          <  2:1103983/149‑4 (MQ=255)
              cTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCaa                                                                                                                                        <  2:480994/149‑1 (MQ=255)
               tATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAAt                                                                                                                                       >  1:918378/1‑149 (MQ=255)
                  tCGCCGCTACGGGTAGTTATTGTGGCGTTGTAGATGGCGGTACAATTCACTTTGTTGTCGAACTGGGGAATGCTTCCTTTTCAGTGAATACTTACTAGGCATACAAGGTTGTCACACTCTGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                    <  2:1170502/149‑1 (MQ=255)
                  tCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                    <  2:125015/149‑1 (MQ=255)
                  tCGCCGCTACGGGTAGTTAGGCTGCCGTTGTAGATGGCGGAAGAATTCAATTTGAACGTGTACTGGTGAATTCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                    <  2:842241/149‑1 (MQ=255)
                   cgccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTg                                                                                                                                   <  2:1037569/149‑1 (MQ=255)
                    gccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGATCTGGTGAAGGCTGACTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGt                                                                                                                                  >  2:654874/1‑149 (MQ=255)
                    gccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCCATGCTGt                                                                                                                                  >  2:1242649/1‑149 (MQ=255)
                     ccgcTACGTGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTAGGCAGACCAGGTTGTCACACTCGGTCAATAGCGTACCGATATTTTCAATGCTGtt                                                                                                                                 <  1:162444/149‑1 (MQ=255)
                       gcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                               >  1:614114/1‑149 (MQ=255)
                       gcTACGGGTAGTTATGCTGCCGTTGTAGATCGCGGTACTATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                               <  2:551005/149‑1 (MQ=255)
                              gTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATAcc                                                                                                                        >  2:47703/1‑149 (MQ=255)
                                tgTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctc                                                                                                                      <  1:474528/148‑1 (MQ=255)
                                 gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGCACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                     <  2:1021016/149‑1 (MQ=255)
                                 gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                     <  1:525506/149‑1 (MQ=255)
                                   tATGCTGCCGTTGTAGATGGCTGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATTCAGTTGGTAATACCTCTGc                                                                                                                   >  1:1049788/1‑149 (MQ=255)
                                   tATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGc                                                                                                                   <  2:918378/149‑1 (MQ=255)
                                    aTGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAACGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTAAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCa                                                                                                                  >  2:1213414/1‑149 (MQ=255)
                                      gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                      <  2:1289644/127‑1 (MQ=255)
                                      gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                      >  1:1289644/1‑127 (MQ=255)
                                      gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAtt                                                                                                                <  2:604953/149‑1 (MQ=255)
                                      gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAtt                                                                                                                >  2:204716/1‑149 (MQ=255)
                                        tgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTaa                                                                                                              >  2:660956/1‑149 (MQ=255)
                                         gcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAt                                                                                                             >  2:470513/1‑149 (MQ=255)
                                          ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                            >  1:544092/1‑149 (MQ=255)
                                          ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                            >  1:1159450/1‑149 (MQ=255)
                                           cGTTGTTGATGGGGGTACAATGCACTTTGAAGGCTAACTGGTGAATGCTGCCTGGTCATTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGGCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                           <  2:1188834/149‑1 (MQ=255)
                                           cGTTGTAGATGGCGGTACAATTCACTTTGAAGGAGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATTTCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                           <  1:664617/149‑1 (MQ=255)
                                                 aGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattca                                                                                                     <  1:684181/149‑1 (MQ=255)
                                                  gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTTCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattaac                                                                                                    >  1:832923/1‑149 (MQ=255)
                                                  gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcac                                                                                                    <  2:1415367/149‑1 (MQ=255)
                                                   aTGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcacc                                                                                                   <  1:1385890/149‑1 (MQ=255)
                                                       cGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCGTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCTATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattc                                                                                               <  2:805223/149‑1 (MQ=255)
                                                        ggTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattca                                                                                              <  1:219745/149‑1 (MQ=255)
                                                         gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                             >  2:69190/1‑149 (MQ=255)
                                                         gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                             <  2:194175/149‑1 (MQ=255)
                                                             aaTTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCTATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGa                                                                                         <  1:1180377/149‑1 (MQ=255)
                                                               ttCACTTTGAAGGCGAACTGGGGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAAc                                                                                       <  2:651652/149‑1 (MQ=255)
                                                                tCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACg                                                                                      >  2:1213960/1‑149 (MQ=255)
                                                                  aCTTTGAAGGCGAACTGGTGAATGCTGCCTGTACAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTACAATGATCTTGGTAATACCTCTCCATTAATTCCATTCACCATTCAGTTGAACGaa                                                                                    >  2:709122/1‑148 (MQ=255)
                                                                    tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATGGTACCGATATTTTCAATGCTGTTTGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                  >  1:792151/1‑149 (MQ=255)
                                                                    tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                  <  1:655635/149‑1 (MQ=255)
                                                                        aaGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATATTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGAt                                                                              >  1:1453511/1‑149 (MQ=255)
                                                                         aGGCGAACTGGTGAATGCTGCCTGTTCAGTGACTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                             <  2:717920/149‑1 (MQ=255)
                                                                         aGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                             <  1:1451020/149‑1 (MQ=255)
                                                                          ggCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATcc                                                                            >  2:1238070/1‑149 (MQ=255)
                                                                             gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGCTACtgt                                                                         >  2:886859/1‑149 (MQ=255)
                                                                             gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCtgt                                                                         >  1:23190/1‑149 (MQ=255)
                                                                                   gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTTTTGc                                                                   >  1:637858/1‑149 (MQ=255)
                                                                                   gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGc                                                                   >  1:1543442/1‑149 (MQ=255)
                                                                                   gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCCAAATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGCACGACTGCGATCCTGTTGTTGc                                                                   >  2:1390412/1‑149 (MQ=255)
                                                                                     gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGCAATACCTCTGCATTAATGCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                 >  1:1014130/1‑149 (MQ=255)
                                                                                     gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                 <  1:270473/149‑1 (MQ=255)
                                                                                           tgcCTGTTCAGTGAATTCTGACTCGGCAGACCAGGTTGTCACACTTGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtg                                                           <  1:1274932/149‑1 (MQ=255)
                                                                                              cTGTTCAGTGAATACTGACTCGGCAGACCAGGTGGGCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctg                                                        <  2:79591/149‑1 (MQ=255)
                                                                                               tGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctgc                                                       >  2:1029993/1‑149 (MQ=255)
                                                                                                 ttCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCg                                                     >  2:566589/1‑149 (MQ=255)
                                                                                                  tCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGt                                                    <  1:470523/149‑1 (MQ=255)
                                                                                                   cAGTGAATACTGACTCGGCAGACCAGGTTGTCACACGCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTCACCATTCAGTTGAACGCCTGCGATCCGGTTTTTGCCGCTATTGCTGCCGtt                                                   >  1:857547/1‑149 (MQ=255)
                                                                                                      tGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTTATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTAGCCGCTAATGCTGCCGTTGCa                                                >  1:721636/1‑149 (MQ=255)
                                                                                                         aTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCAttt                                             >  2:71516/1‑149 (MQ=255)
                                                                                                           aCTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTc                                           >  1:286396/1‑149 (MQ=255)
                                                                                                           aCTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATTTTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTAACCATTCAGTTGAACGACTGCGCTCCTGTTGTTGCCGCTAATGCTGCCGTTGCAGTTTc                                           >  2:137190/1‑149 (MQ=255)
                                                                                                            cTGCCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTACATTCAACATTCAGTTTAACGACTGCGATCATGGTGTTGCCGCTAATGCTGCCGTTGCATTTTcg                                          >  2:310725/1‑148 (MQ=255)
                                                                                                            cTGACTCGGCAGACCAGGTTGTCACCCTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCAGTAATTCCATTCCCCATTCAGTTGCCCGCCTGCGCTACTGTTGTTTCCGCGAATGCTGCCGTTGCAATTTcg                                          >  2:1447722/1‑148 (MQ=255)
                                                                                                             tGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCTTTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTg                                         <  1:750591/149‑1 (MQ=255)
                                                                                                             tGACTCGGCAGACCAGGGTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTg                                         <  1:1493463/149‑1 (MQ=255)
                                                                                                               aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGCCTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTggg                                       >  1:1422423/1‑148 (MQ=255)
                                                                                                               aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGt                                       <  1:519052/149‑1 (MQ=255)
                                                                                                               aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTGGAACGCCGGCGATCGTGGTGTTGGCGCTAATGCTGGGGTTGCTGTGTCTGGt                                       >  2:1071310/1‑149 (MQ=255)
                                                                                                                 tCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCa                                     >  1:1173947/1‑149 (MQ=255)
                                                                                                                   ggCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAgg                                   <  1:450163/149‑1 (MQ=255)
                                                                                                                         ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                             <  1:43829/149‑1 (MQ=255)
                                                                                                                         ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCACTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                             <  1:204716/149‑1 (MQ=255)
                                                                                                                                gTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAGTAAGTCCATTGACCTTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGGTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatgaa                      >  1:1016526/1‑149 (MQ=255)
                                                                                                                                 tCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatcaat                     <  2:1159450/149‑1 (MQ=255)
                                                                                                                                  cacaCTCGGTCAATATCGTACCGATATTTTCAATGCTTTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATg                    <  1:254815/149‑1 (MQ=255)
                                                                                                                                     acTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTTCATTTTCTGGTCAGGCTGATGCAATCAATGATa                 >  1:281117/1‑149 (MQ=255)
                                                                                                                                       tCGGTCAATATCGTACCGATATTTTGAATGCTGTTTGTAATACCTCTGCATTAATTACAATCCCCCTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt               <  2:255399/149‑1 (MQ=255)
                                                                                                                                       attgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt               >  1:318367/7‑149 (MQ=255)
                                                                                                                                        ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATCCCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAtt              >  1:1049056/6‑149 (MQ=255)
                                                                                                                                        ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATAACTCTGCATTTATTCCATTCACAATTCAGTTGACCGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCTTTTTCTGGTCAGGCTGATGCATTCAATGCTAAtt              >  1:1045015/6‑149 (MQ=255)
                                                                                                                                        agggccatatCTTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTTCGATCCTGTTGTTGCAGCTAATGCTGCCGTGGCATTTTCTGGTCCGGCTGATGCAATCAATGATAAtt              <  1:910244/143‑1 (MQ=255)
                                                                                                                                         tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt             >  2:715284/5‑149 (MQ=255)
                                                                                                                                         tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt             >  1:116605/5‑149 (MQ=255)
                                                                                                                                          gTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTATTTCCATTCACCAGTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATTAtttt            >  1:367475/1‑148 (MQ=255)
                                                                                                                                            cAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATACCATTCACCATTCAGTTGAACGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAAACAATGATAATTTAtt          >  1:1534406/1‑149 (MQ=255)
                                                                                                                                             aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTTCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg         <  1:727363/149‑1 (MQ=255)
                                                                                                                                             aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCTCCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGGATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg         >  1:515805/1‑149 (MQ=255)
                                                                                                                                             aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg         <  1:1449718/149‑1 (MQ=255)
                                                                                                                                             aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg         >  1:682118/1‑149 (MQ=255)
                                                                                                                                              atatTGTCCCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTGAGTTGAACGACTGCGATCCTGTTGTTGCCGCTACTGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTgg        <  1:102901/149‑1 (MQ=255)
                                                                                                                                                    tACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGGTGCATTTTCTGGTCAGGCTTATGCAATCAATTATAATTTATTGGCCAtta  >  2:863903/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTG  >  NC_000913/558237‑558533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: