Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 558,386 A→T 6.9% T59S (ACC→TCC) ‡ sfmA → FimA homolog, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913558,3860AT 6.9% 277.2 / 8.7 102T59S (ACC→TCC) ‡sfmAFimA homolog, function unknown
Reads supporting (aligned to +/- strand):  ref base A (47/47);  new base T (5/2);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 104 total aligned reads displayed.

GCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGC  >  NC_000913/558238‑558534
                                                                                                                                                    |                                                                                                                                                    
gctggctgCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTa                                                                                                                                                      <  1:1077415/149‑1 (MQ=255)
 ctggctgCCGCACTATTCTCCGCTACGTGTAGTAATGCTGCCGTTGTAGATGGATGTACAATTCACTTAGAAGGCGAAATGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTAc                                                                                                                                                     <  2:935969/149‑1 (MQ=255)
    gctgCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGa                                                                                                                                                  <  1:1560948/149‑1 (MQ=255)
        ccGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGCTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAAAATCTTAACGAAAtt                                                                                                                                              >  2:254815/1‑149 (MQ=255)
          ccACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttga                                                                                                                                            <  1:1333839/148‑3 (MQ=255)
           cACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttgac                                                                                                                                           <  2:1103983/149‑4 (MQ=255)
             cTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCaa                                                                                                                                         <  2:480994/149‑1 (MQ=255)
              tATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAAt                                                                                                                                        >  1:918378/1‑149 (MQ=255)
                 tCGCCGCTACGGGTAGTTATTGTGGCGTTGTAGATGGCGGTACAATTCACTTTGTTGTCGAACTGGGGAATGCTTCCTTTTCAGTGAATACTTACTAGGCATACAAGGTTGTCACACTCTGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:1170502/149‑1 (MQ=255)
                 tCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:125015/149‑1 (MQ=255)
                 tCGCCGCTACGGGTAGTTAGGCTGCCGTTGTAGATGGCGGAAGAATTCAATTTGAACGTGTACTGGTGAATTCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:842241/149‑1 (MQ=255)
                  cgccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTg                                                                                                                                    <  2:1037569/149‑1 (MQ=255)
                   gccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGATCTGGTGAAGGCTGACTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGt                                                                                                                                   >  2:654874/1‑149 (MQ=255)
                   gccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCCATGCTGt                                                                                                                                   >  2:1242649/1‑149 (MQ=255)
                    ccgcTACGTGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTAGGCAGACCAGGTTGTCACACTCGGTCAATAGCGTACCGATATTTTCAATGCTGtt                                                                                                                                  <  1:162444/149‑1 (MQ=255)
                      gcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                                >  1:614114/1‑149 (MQ=255)
                      gcTACGGGTAGTTATGCTGCCGTTGTAGATCGCGGTACTATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                                <  2:551005/149‑1 (MQ=255)
                             gTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATAcc                                                                                                                         >  2:47703/1‑149 (MQ=255)
                               tgTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctc                                                                                                                       <  1:474528/148‑1 (MQ=255)
                                gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGCACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                      <  2:1021016/149‑1 (MQ=255)
                                gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                      <  1:525506/149‑1 (MQ=255)
                                  tATGCTGCCGTTGTAGATGGCTGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATTCAGTTGGTAATACCTCTGc                                                                                                                    >  1:1049788/1‑149 (MQ=255)
                                  tATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGc                                                                                                                    <  2:918378/149‑1 (MQ=255)
                                   aTGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAACGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTAAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCa                                                                                                                   >  2:1213414/1‑149 (MQ=255)
                                     gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                       <  2:1289644/127‑1 (MQ=255)
                                     gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                       >  1:1289644/1‑127 (MQ=255)
                                     gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAtt                                                                                                                 <  2:604953/149‑1 (MQ=255)
                                       tgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTaa                                                                                                               >  2:660956/1‑149 (MQ=255)
                                        gcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAt                                                                                                              >  2:470513/1‑149 (MQ=255)
                                         ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                             >  1:1159450/1‑149 (MQ=255)
                                         ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                             >  1:544092/1‑149 (MQ=255)
                                          cGTTGTTGATGGGGGTACAATGCACTTTGAAGGCTAACTGGTGAATGCTGCCTGGTCATTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGGCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                            <  2:1188834/149‑1 (MQ=255)
                                          cGTTGTAGATGGCGGTACAATTCACTTTGAAGGAGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATTTCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                            <  1:664617/149‑1 (MQ=255)
                                              gTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccatt                                                                                                        <  2:238528/149‑1 (MQ=255)
                                                aGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattca                                                                                                      <  1:684181/149‑1 (MQ=255)
                                                 gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTTCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattaac                                                                                                     >  1:832923/1‑149 (MQ=255)
                                                 gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcac                                                                                                     <  2:1415367/149‑1 (MQ=255)
                                                  aTGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcacc                                                                                                    <  1:1385890/149‑1 (MQ=255)
                                                      cGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCGTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCTATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattc                                                                                                <  2:805223/149‑1 (MQ=255)
                                                       ggTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattca                                                                                               <  1:219745/149‑1 (MQ=255)
                                                        gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                              <  2:194175/149‑1 (MQ=255)
                                                        gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                              >  2:69190/1‑149 (MQ=255)
                                                            aaTTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCTATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGa                                                                                          <  1:1180377/149‑1 (MQ=255)
                                                              ttCACTTTGAAGGCGAACTGGGGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAAc                                                                                        <  2:651652/149‑1 (MQ=255)
                                                               tCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACg                                                                                       >  2:1213960/1‑149 (MQ=255)
                                                                 aCTTTGAAGGCGAACTGGTGAATGCTGCCTGTACAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTACAATGATCTTGGTAATACCTCTCCATTAATTCCATTCACCATTCAGTTGAACGaa                                                                                     >  2:709122/1‑148 (MQ=255)
                                                                   tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATGGTACCGATATTTTCAATGCTGTTTGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                   >  1:792151/1‑149 (MQ=255)
                                                                   tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                   <  1:655635/149‑1 (MQ=255)
                                                                       aaGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATATTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGAt                                                                               >  1:1453511/1‑149 (MQ=255)
                                                                        aGGCGAACTGGTGAATGCTGCCTGTTCAGTGACTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                              <  2:717920/149‑1 (MQ=255)
                                                                        aGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                              <  1:1451020/149‑1 (MQ=255)
                                                                         ggCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATcc                                                                             >  2:1238070/1‑149 (MQ=255)
                                                                            gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGCTACtgt                                                                          >  2:886859/1‑149 (MQ=255)
                                                                            gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCtgt                                                                          >  1:23190/1‑149 (MQ=255)
                                                                                  gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTTTTGc                                                                    >  1:637858/1‑149 (MQ=255)
                                                                                  gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGc                                                                    >  1:1543442/1‑149 (MQ=255)
                                                                                  gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCCAAATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGCACGACTGCGATCCTGTTGTTGc                                                                    >  2:1390412/1‑149 (MQ=255)
                                                                                    gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGCAATACCTCTGCATTAATGCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                  >  1:1014130/1‑149 (MQ=255)
                                                                                    gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                  <  1:270473/149‑1 (MQ=255)
                                                                                          tgcCTGTTCAGTGAATTCTGACTCGGCAGACCAGGTTGTCACACTTGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtg                                                            <  1:1274932/149‑1 (MQ=255)
                                                                                             cTGTTCAGTGAATACTGACTCGGCAGACCAGGTGGGCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctg                                                         <  2:79591/149‑1 (MQ=255)
                                                                                              tGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctgc                                                        >  2:1029993/1‑149 (MQ=255)
                                                                                                ttCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCg                                                      >  2:566589/1‑149 (MQ=255)
                                                                                                 tCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGt                                                     <  1:470523/149‑1 (MQ=255)
                                                                                                  cAGTGAATACTGACTCGGCAGACCAGGTTGTCACACGCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTCACCATTCAGTTGAACGCCTGCGATCCGGTTTTTGCCGCTATTGCTGCCGtt                                                    >  1:857547/1‑149 (MQ=255)
                                                                                                     tGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTTATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTAGCCGCTAATGCTGCCGTTGCa                                                 >  1:721636/1‑149 (MQ=255)
                                                                                                        aTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCAttt                                              >  2:71516/1‑149 (MQ=255)
                                                                                                          aCTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTc                                            >  1:286396/1‑149 (MQ=255)
                                                                                                          aCTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATTTTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTAACCATTCAGTTGAACGACTGCGCTCCTGTTGTTGCCGCTAATGCTGCCGTTGCAGTTTc                                            >  2:137190/1‑149 (MQ=255)
                                                                                                           cTGCCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTACATTCAACATTCAGTTTAACGACTGCGATCATGGTGTTGCCGCTAATGCTGCCGTTGCATTTTcg                                           >  2:310725/1‑148 (MQ=255)
                                                                                                           cTGACTCGGCAGACCAGGTTGTCACCCTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCAGTAATTCCATTCCCCATTCAGTTGCCCGCCTGCGCTACTGTTGTTTCCGCGAATGCTGCCGTTGCAATTTcg                                           >  2:1447722/1‑148 (MQ=255)
                                                                                                            tgagtaggCAGAGCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCTATGCTGTTGGTATTTCCTCTTCATTTATTCCATTCACCATTCAGTTGAAAGACTGCGATCCTGTTGTTGCCGCTATTGCTGCAGTTGCATTTTCTg                                          <  1:771936/143‑1 (MQ=255)
                                                                                                            tGACTCGGCAGACCAGGGTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTg                                          <  1:1493463/149‑1 (MQ=255)
                                                                                                              aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGCCTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTggg                                        >  1:1422423/1‑148 (MQ=255)
                                                                                                              aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGt                                        <  1:519052/149‑1 (MQ=255)
                                                                                                              aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTGGAACGCCGGCGATCGTGGTGTTGGCGCTAATGCTGGGGTTGCTGTGTCTGGt                                        >  2:1071310/1‑149 (MQ=255)
                                                                                                                tCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCa                                      >  1:1173947/1‑149 (MQ=255)
                                                                                                                  ggCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAgg                                    <  1:450163/149‑1 (MQ=255)
                                                                                                                        ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                              <  1:43829/149‑1 (MQ=255)
                                                                                                                        ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCACTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                              <  1:204716/149‑1 (MQ=255)
                                                                                                                               gTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAGTAAGTCCATTGACCTTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGGTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatgaa                       >  1:1016526/1‑149 (MQ=255)
                                                                                                                                tCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatcaat                      <  2:1159450/149‑1 (MQ=255)
                                                                                                                                 cacaCTCGGTCAATATCGTACCGATATTTTCAATGCTTTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATg                     <  1:254815/149‑1 (MQ=255)
                                                                                                                                    acTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTTCATTTTCTGGTCAGGCTGATGCAATCAATGATa                  >  1:281117/1‑149 (MQ=255)
                                                                                                                                      tCGGTCAATATCGTACCGATATTTTGAATGCTGTTTGTAATACCTCTGCATTAATTACAATCCCCCTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt                <  2:255399/149‑1 (MQ=255)
                                                                                                                                      attgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt                >  1:318367/7‑149 (MQ=255)
                                                                                                                                       ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATCCCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAtt               >  1:1049056/6‑149 (MQ=255)
                                                                                                                                       ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATAACTCTGCATTTATTCCATTCACAATTCAGTTGACCGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCTTTTTCTGGTCAGGCTGATGCATTCAATGCTAAtt               >  1:1045015/6‑149 (MQ=255)
                                                                                                                                       agggccatatCTTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTTCGATCCTGTTGTTGCAGCTAATGCTGCCGTGGCATTTTCTGGTCCGGCTGATGCAATCAATGATAAtt               <  1:910244/143‑1 (MQ=255)
                                                                                                                                        tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt              >  2:715284/5‑149 (MQ=255)
                                                                                                                                        tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt              >  1:116605/5‑149 (MQ=255)
                                                                                                                                         gTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTATTTCCATTCACCAGTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATTAtttt             >  1:367475/1‑148 (MQ=255)
                                                                                                                                           cAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATACCATTCACCATTCAGTTGAACGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAAACAATGATAATTTAtt           >  1:1534406/1‑149 (MQ=255)
                                                                                                                                            aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTTCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          <  1:727363/149‑1 (MQ=255)
                                                                                                                                            aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCTCCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGGATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          >  1:515805/1‑149 (MQ=255)
                                                                                                                                            aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          <  1:1449718/149‑1 (MQ=255)
                                                                                                                                            aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          >  1:682118/1‑149 (MQ=255)
                                                                                                                                             atatTGTCCCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTGAGTTGAACGACTGCGATCCTGTTGTTGCCGCTACTGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTgg         <  1:102901/149‑1 (MQ=255)
                                                                                                                                                   tACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGGTGCATTTTCTGGTCAGGCTTATGCAATCAATTATAATTTATTGGCCAtt  >  2:863903/1‑148 (MQ=255)
                                                                                                                                                    aCCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCAGCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGc  <  1:626339/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGC  >  NC_000913/558238‑558534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: