Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 558,387 C→A 7.4% T59N (ACC→AAC) ‡ sfmA → FimA homolog, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913558,3870CA 7.4% 252.0 / 6.6 102T59N (ACC→AAC) ‡sfmAFimA homolog, function unknown
Reads supporting (aligned to +/- strand):  ref base C (46/48);  new base A (6/2);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 103 total aligned reads displayed.

CTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGC  >  NC_000913/558239‑558534
                                                                                                                                                    |                                                                                                                                                   
ctggctgCCGCACTATTCTCCGCTACGTGTAGTAATGCTGCCGTTGTAGATGGATGTACAATTCACTTAGAAGGCGAAATGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTAc                                                                                                                                                     <  2:935969/149‑1 (MQ=255)
   gctgCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGa                                                                                                                                                  <  1:1560948/149‑1 (MQ=255)
       ccGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGCTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAAAATCTTAACGAAAtt                                                                                                                                              >  2:254815/1‑149 (MQ=255)
         ccACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttga                                                                                                                                            <  1:1333839/148‑3 (MQ=255)
          cACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGGTACGATAttgac                                                                                                                                           <  2:1103983/149‑4 (MQ=255)
            cTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCaa                                                                                                                                         <  2:480994/149‑1 (MQ=255)
             tATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAAt                                                                                                                                        >  1:918378/1‑149 (MQ=255)
                tCGCCGCTACGGGTAGTTATTGTGGCGTTGTAGATGGCGGTACAATTCACTTTGTTGTCGAACTGGGGAATGCTTCCTTTTCAGTGAATACTTACTAGGCATACAAGGTTGTCACACTCTGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:1170502/149‑1 (MQ=255)
                tCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:125015/149‑1 (MQ=255)
                tCGCCGCTACGGGTAGTTAGGCTGCCGTTGTAGATGGCGGAAGAATTCAATTTGAACGTGTACTGGTGAATTCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCt                                                                                                                                     <  2:842241/149‑1 (MQ=255)
                 cgccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTg                                                                                                                                    <  2:1037569/149‑1 (MQ=255)
                  gccgcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGATCTGGTGAAGGCTGACTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGt                                                                                                                                   >  2:654874/1‑149 (MQ=255)
                   ccgcTACGTGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTAGGCAGACCAGGTTGTCACACTCGGTCAATAGCGTACCGATATTTTCAATGCTGtt                                                                                                                                  <  1:162444/149‑1 (MQ=255)
                     gcTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                                >  1:614114/1‑149 (MQ=255)
                     gcTACGGGTAGTTATGCTGCCGTTGTAGATCGCGGTACTATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTgg                                                                                                                                <  2:551005/149‑1 (MQ=255)
                            gTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATAcc                                                                                                                         >  2:47703/1‑149 (MQ=255)
                              tgTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctc                                                                                                                       <  1:474528/148‑1 (MQ=255)
                               gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGCACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                      <  2:1021016/149‑1 (MQ=255)
                               gTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACctct                                                                                                                      <  1:525506/149‑1 (MQ=255)
                                 tATGCTGCCGTTGTAGATGGCTGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATTCAGTTGGTAATACCTCTGc                                                                                                                    >  1:1049788/1‑149 (MQ=255)
                                 tATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGc                                                                                                                    <  2:918378/149‑1 (MQ=255)
                                  aTGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAACGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTAAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCa                                                                                                                   >  2:1213414/1‑149 (MQ=255)
                                    gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                       <  2:1289644/127‑1 (MQ=255)
                                    gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATg                                                                                                                                       >  1:1289644/1‑127 (MQ=255)
                                    gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAtt                                                                                                                 <  2:604953/149‑1 (MQ=255)
                                    gctgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAtt                                                                                                                 >  2:204716/1‑149 (MQ=255)
                                      tgcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTaa                                                                                                               >  2:660956/1‑149 (MQ=255)
                                       gcCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAt                                                                                                              >  2:470513/1‑149 (MQ=255)
                                        ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                             >  1:1159450/1‑149 (MQ=255)
                                        ccGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAAtt                                                                                                             >  1:544092/1‑149 (MQ=255)
                                         cGTTGTTGATGGGGGTACAATGCACTTTGAAGGCTAACTGGTGAATGCTGCCTGGTCATTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGGCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                            <  2:1188834/149‑1 (MQ=255)
                                         cGTTGTAGATGGCGGTACAATTCACTTTGAAGGAGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATTTCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTc                                                                                                            <  1:664617/149‑1 (MQ=255)
                                             gTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccatt                                                                                                        <  2:238528/149‑1 (MQ=255)
                                               aGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattca                                                                                                      <  1:684181/149‑1 (MQ=255)
                                                gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTTCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattaac                                                                                                     >  1:832923/1‑149 (MQ=255)
                                                gATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcac                                                                                                     <  2:1415367/149‑1 (MQ=255)
                                                 aTGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcacc                                                                                                    <  1:1385890/149‑1 (MQ=255)
                                                     cGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCGTGTTCAGTGAATACTGACTCGGCAGACAAGGTTGTCACACTCGGTCAATATCGTACCTATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattc                                                                                                <  2:805223/149‑1 (MQ=255)
                                                      ggTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTccattcaccattca                                                                                               <  1:219745/149‑1 (MQ=255)
                                                       gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                              <  2:194175/149‑1 (MQ=255)
                                                       gTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAg                                                                                              >  2:69190/1‑149 (MQ=255)
                                                           aaTTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCTATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGa                                                                                          <  1:1180377/149‑1 (MQ=255)
                                                             ttCACTTTGAAGGCGAACTGGGGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAAc                                                                                        <  2:651652/149‑1 (MQ=255)
                                                              tCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACg                                                                                       >  2:1213960/1‑149 (MQ=255)
                                                                aCTTTGAAGGCGAACTGGTGAATGCTGCCTGTACAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTACAATGATCTTGGTAATACCTCTCCATTAATTCCATTCACCATTCAGTTGAACGaa                                                                                     >  2:709122/1‑148 (MQ=255)
                                                                  tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATGGTACCGATATTTTCAATGCTGTTTGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                   >  1:792151/1‑149 (MQ=255)
                                                                  tttGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTg                                                                                   <  1:655635/149‑1 (MQ=255)
                                                                      aaGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATATTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGAt                                                                               >  1:1453511/1‑149 (MQ=255)
                                                                       aGGCGAACTGGTGAATGCTGCCTGTTCAGTGACTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                              <  2:717920/149‑1 (MQ=255)
                                                                       aGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATc                                                                              <  1:1451020/149‑1 (MQ=255)
                                                                        ggCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATcc                                                                             >  2:1238070/1‑149 (MQ=255)
                                                                           gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGCTACtgt                                                                          >  2:886859/1‑149 (MQ=255)
                                                                           gAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCtgt                                                                          >  1:23190/1‑149 (MQ=255)
                                                                                 gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTTTTGc                                                                    >  1:637858/1‑149 (MQ=255)
                                                                                 gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGc                                                                    >  1:1543442/1‑149 (MQ=255)
                                                                                 gTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCCAAATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGCACGACTGCGATCCTGTTGTTGc                                                                    >  2:1390412/1‑149 (MQ=255)
                                                                                   gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCCCACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGCAATACCTCTGCATTAATGCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                  >  1:1014130/1‑149 (MQ=255)
                                                                                   gAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCg                                                                  <  1:270473/149‑1 (MQ=255)
                                                                                         tgcCTGTTCAGTGAATTCTGACTCGGCAGACCAGGTTGTCACACTTGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtg                                                            <  1:1274932/149‑1 (MQ=255)
                                                                                            cTGTTCAGTGAATACTGACTCGGCAGACCAGGTGGGCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctg                                                         <  2:79591/149‑1 (MQ=255)
                                                                                             tGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAAtgctgc                                                        >  2:1029993/1‑149 (MQ=255)
                                                                                               ttCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCg                                                      >  2:566589/1‑149 (MQ=255)
                                                                                                tCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGt                                                     <  1:470523/149‑1 (MQ=255)
                                                                                                 cAGTGAATACTGACTCGGCAGACCAGGTTGTCACACGCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTCACCATTCAGTTGAACGCCTGCGATCCGGTTTTTGCCGCTATTGCTGCCGtt                                                    >  1:857547/1‑149 (MQ=255)
                                                                                                    tGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTTATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTAGCCGCTAATGCTGCCGTTGCa                                                 >  1:721636/1‑149 (MQ=255)
                                                                                                       aTACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCAttt                                              >  2:71516/1‑149 (MQ=255)
                                                                                                         aCTGACTCGGCAGCCCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTc                                            >  1:286396/1‑149 (MQ=255)
                                                                                                         aCTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATTTTTTCAATGCTGTTGGTAATACCTCTGCATTTATTCCATTAACCATTCAGTTGAACGACTGCGCTCCTGTTGTTGCCGCTAATGCTGCCGTTGCAGTTTc                                            >  2:137190/1‑149 (MQ=255)
                                                                                                          cTGCCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTACATTCAACATTCAGTTTAACGACTGCGATCATGGTGTTGCCGCTAATGCTGCCGTTGCATTTTcg                                           >  2:310725/1‑148 (MQ=255)
                                                                                                          cTGACTCGGCAGACCAGGTTGTCACCCTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCGCTGCAGTAATTCCATTCCCCATTCAGTTGCCCGCCTGCGCTACTGTTGTTTCCGCGAATGCTGCCGTTGCAATTTcg                                           >  2:1447722/1‑148 (MQ=255)
                                                                                                           tgagtaggCAGAGCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCTATGCTGTTGGTATTTCCTCTTCATTTATTCCATTCACCATTCAGTTGAAAGACTGCGATCCTGTTGTTGCCGCTATTGCTGCAGTTGCATTTTCTg                                          <  1:771936/143‑1 (MQ=255)
                                                                                                           tGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCTTTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTg                                          <  1:750591/149‑1 (MQ=255)
                                                                                                           tGACTCGGCAGACCAGGGTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTg                                          <  1:1493463/149‑1 (MQ=255)
                                                                                                             aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGCCTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTggg                                        >  1:1422423/1‑148 (MQ=255)
                                                                                                             aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGt                                        <  1:519052/149‑1 (MQ=255)
                                                                                                             aCTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTGGAACGCCGGCGATCGTGGTGTTGGCGCTAATGCTGGGGTTGCTGTGTCTGGt                                        >  2:1071310/1‑149 (MQ=255)
                                                                                                               tCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCa                                      >  1:1173947/1‑149 (MQ=255)
                                                                                                                       ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                              <  1:43829/149‑1 (MQ=255)
                                                                                                                       ccAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCACTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATg                              <  1:204716/149‑1 (MQ=255)
                                                                                                                              gTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatcaa                       >  1:207694/1‑149 (MQ=255)
                                                                                                                              gTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCAGTAAGTCCATTGACCTTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGGTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatgaa                       >  1:1016526/1‑149 (MQ=255)
                                                                                                                               tCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGcaatcaat                      <  2:1159450/149‑1 (MQ=255)
                                                                                                                                cacaCTCGGTCAATATCGTACCGATATTTTCAATGCTTTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATg                     <  1:254815/149‑1 (MQ=255)
                                                                                                                                   acTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTTCATTTTCTGGTCAGGCTGATGCAATCAATGATa                  >  1:281117/1‑149 (MQ=255)
                                                                                                                                     tCGGTCAATATCGTACCGATATTTTGAATGCTGTTTGTAATACCTCTGCATTAATTACAATCCCCCTTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt                <  2:255399/149‑1 (MQ=255)
                                                                                                                                     attgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAt                >  1:318367/7‑149 (MQ=255)
                                                                                                                                      ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATCCCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAtt               >  1:1049056/6‑149 (MQ=255)
                                                                                                                                      ttgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATAACTCTGCATTTATTCCATTCACAATTCAGTTGACCGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCTTTTTCTGGTCAGGCTGATGCATTCAATGCTAAtt               >  1:1045015/6‑149 (MQ=255)
                                                                                                                                      agggccatatCTTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGACCGACTTCGATCCTGTTGTTGCAGCTAATGCTGCCGTGGCATTTTCTGGTCCGGCTGATGCAATCAATGATAAtt               <  1:910244/143‑1 (MQ=255)
                                                                                                                                       tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt              >  1:116605/5‑149 (MQ=255)
                                                                                                                                       tgaaaaTATCGGTACGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAAttt              >  2:715284/5‑149 (MQ=255)
                                                                                                                                        gTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTATTTCCATTCACCAGTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATTAtttt             >  1:367475/1‑148 (MQ=255)
                                                                                                                                          cAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATACCATTCACCATTCAGTTGAACGACTGCGATACTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAAACAATGATAATTTAtt           >  1:1534406/1‑149 (MQ=255)
                                                                                                                                           aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTTCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          <  1:727363/149‑1 (MQ=255)
                                                                                                                                           aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCTCCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGGATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          >  1:515805/1‑149 (MQ=255)
                                                                                                                                           aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          <  1:1449718/149‑1 (MQ=255)
                                                                                                                                           aaTATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTg          >  1:682118/1‑149 (MQ=255)
                                                                                                                                            atatTGTCCCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTGAGTTGAACGACTGCGATCCTGTTGTTGCCGCTACTGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTgg         <  1:102901/149‑1 (MQ=255)
                                                                                                                                                  tACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGGTGCATTTTCTGGTCAGGCTTATGCAATCAATTATAATTTATTGGCCAtta   >  2:863903/1‑148 (MQ=255)
                                                                                                                                                   aCCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCAGCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGc  <  1:626339/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGGCTGCCGCACTATTCGCCGCTACGGGTAGTTATGCTGCCGTTGTAGATGGCGGTACAATTCACTTTGAAGGCGAACTGGTGAATGCTGCCTGTTCAGTGAATACTGACTCGGCAGACCAGGTTGTCACACTCGGTCAATATCGTACCGATATTTTCAATGCTGTTGGTAATACCTCTGCATTAATTCCATTCACCATTCAGTTGAACGACTGCGATCCTGTTGTTGCCGCTAATGCTGCCGTTGCATTTTCTGGTCAGGCTGATGCAATCAATGATAATTTATTGGCCATTGC  >  NC_000913/558239‑558534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑