Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 824,572 T→A 6.2% intergenic (‑75/‑58) ybhP ← / → ybhQ endo/exonuclease/phosphatase family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913824,5720TA 6.2% 244.0 / 2.4 86intergenic (‑75/‑58)ybhP/ybhQendo/exonuclease/phosphatase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (36/43);  new base A (3/3);  total (39/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGT  >  NC_000913/824424‑824718
                                                                                                                                                    |                                                                                                                                                  
ttAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAt                                                                                                                                                    >  1:415781/1‑149 (MQ=255)
   aaCTCGGTATATCCTTTTTTAATAATGATGTTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAtttt                                                                                                                                                 <  2:702310/149‑1 (MQ=255)
     ctcgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGCTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTcc                                                                                                                                               <  1:737383/147‑1 (MQ=255)
       cgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTTTTTGATCGGGCATAATTTTCATGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGt                                                                                                                                             <  2:761066/149‑1 (MQ=255)
         gTAAAGCCTTTGTGAATATTGATGCTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaa                                                                                                                                           <  1:1360/149‑1 (MQ=255)
          tAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGTAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTAGCCTCTTATCTCATTGAAATAGTGTAGTAGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                          <  2:389473/149‑1 (MQ=255)
          tAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAATGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                          <  2:506821/149‑1 (MQ=255)
          tAAAGCCTTTGTGAATATTGATGGTGAGCAACTTGAACGAAAATTGGTGGGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTaaa                                                                                                                                          <  1:1396834/149‑1 (MQ=255)
           aaaTCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGGCTTACTGAAAATTCCGTaaga                                                                                                                                         <  2:94582/149‑3 (MQ=255)
           aaaGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTaaga                                                                                                                                         <  2:25946/149‑3 (MQ=255)
             tgacTTTCTGAATAATTATTGTGATCACCTTGAATTTAAATTGTTGTGTTTTTTCTGTCATATTTTTCCTGGCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCTGCGTCACAAAAAGGTGCTGTCTTACGGAATTTTCCGTAAAGtt                                                                                                                                       <  1:1062048/146‑1 (MQ=255)
             aGCCTTTGTGAATATTGAAGGTGAGCACCTTGAACGAAAATTCTTGTGTATGATCGGGCCTAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGtt                                                                                                                                       <  1:1435630/149‑1 (MQ=255)
               ccTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGGCACAAAAAGGTGCGGTCTTACGGAAATTTCCGTAAAGTTCg                                                                                                                                     <  1:736840/149‑1 (MQ=255)
                cTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAATTAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTTCGGAATTTTCCGTAAAGTTCgg                                                                                                                                    >  1:496697/1‑149 (MQ=255)
                    gtgAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTAct                                                                                                                                <  2:1233795/149‑1 (MQ=255)
                     tgAATATTGATGGTGAGCACCTTGATCGAAAATTGTTGTGTTTGATCTGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                                                               <  2:543569/149‑1 (MQ=255)
                     tgAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                                                               >  2:1378462/1‑149 (MQ=255)
                     tgAATATTGATGGTGAGCACCTTGAACGAAAATGGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                                                               <  1:135296/149‑1 (MQ=255)
                      taaTATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                              <  1:766353/148‑1 (MQ=255)
                      gAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTTTTTGAGCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                              <  2:700864/149‑1 (MQ=255)
                        atatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGa                                                                                                                            <  2:238471/149‑1 (MQ=255)
                         tatTGATGGTGAGCACCTTTAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGag                                                                                                                           <  2:240540/149‑1 (MQ=255)
                          atTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGTGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                                                                          <  2:51641/149‑1 (MQ=255)
                          atTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                                                                          <  1:1091059/149‑1 (MQ=255)
                           ttGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagta                                                                                                                         >  2:281811/1‑149 (MQ=255)
                            tGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaa                                                                                                                        <  1:1545260/149‑1 (MQ=255)
                             gATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGCTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACCAAAAGGTGCGGTCTAACGGAAATTTCCGTAAAGTTCGGTACGCTGaggaat                                                                                                                       >  1:512419/1‑148 (MQ=255)
                             gATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaag                                                                                                                       <  1:1556870/149‑1 (MQ=255)
                              aTGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGaggaagt                                                                                                                      >  2:1447612/1‑149 (MQ=255)
                                ggTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATTGTGTAGTCGGCGTCACAAAATGGTGCGGTCTTACGGTATGTTCAGTAAAGTTCGGTACTCTGAGTAAGTag                                                                                                                    >  1:1455635/1‑149 (MQ=255)
                                ggTGAGCAACTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTag                                                                                                                    <  2:477871/149‑1 (MQ=255)
                                  ggAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGAGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTagag                                                                                                                  <  2:971044/148‑1 (MQ=255)
                                      cACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATaa                                                                                                              >  1:1231751/1‑149 (MQ=255)
                                         cTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAtt                                                                                                           >  2:458347/1‑149 (MQ=255)
                                          ttGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAtta                                                                                                          >  1:1355563/1‑148 (MQ=255)
                                            gAACGAAAATTTTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTCCGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttctt                                                                                                        <  2:95996/149‑1 (MQ=255)
                                             aaCGAAAATTGTTGTGTTTGATCGGTCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttctta                                                                                                       >  2:707703/1‑148 (MQ=255)
                                               cGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAg                                                                                                     <  2:95805/149‑1 (MQ=255)
                                                gAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                                                                    >  2:82465/1‑149 (MQ=255)
                                                  aaaTTGTTGTGTTTGATCGGGCATAAATTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACAGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgag                                                                                                  <  1:1568372/149‑1 (MQ=255)
                                                        ttgtGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGc                                                                                            >  1:766315/1‑149 (MQ=255)
                                                        ttgtGTGTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCAGGCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGc                                                                                            <  2:1428211/149‑1 (MQ=255)
                                                            gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATg                                                                                        >  1:1541307/1‑149 (MQ=255)
                                                             tttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTGATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACTGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGa                                                                                       >  1:483702/1‑149 (MQ=255)
                                                             tttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGa                                                                                       >  2:1374254/1‑149 (MQ=255)
                                                              ttGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATTGAGTAGTGGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATACTTCAGGAGAGCAGCCATGaa                                                                                      >  2:116329/1‑149 (MQ=255)
                                                                 ttCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTg                                                                                   <  2:183190/148‑1 (MQ=255)
                                                                 aTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTGACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTAGGTACGATGATTAAGTAGAGATAAATTCTTCAGGAGAGAAGCAATGAAGtc                                                                                   >  2:112878/1‑148 (MQ=255)
                                                                     ggCCTAATTTTCATGTCTTTGCCTCCTCTCTCAATGAAATAGTGTAGTCTTCGTAACAAAAAGGGGCGGTCTTACGGAATTTTCCGTAAAGTTAGGTACTCTGAGTAAGTAGAGATAAATTCTTCCGGAGAGAAGCCATGAAGTGGcaa                                                                               <  2:919562/149‑1 (MQ=255)
                                                                          aaTTTTCCTGTCTTTGCCTCTTATATCATAGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCTTAAAGTTAGGAACTATGAGGAAGTATAGATAACTTCGTCATGAGAGAAGCCATGAAGTTCAAACAACg                                                                          >  1:1062077/1‑149 (MQ=255)
                                                                            ttttCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGAACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGACATGAAGTGGCAACAACgtg                                                                        >  2:1440947/1‑149 (MQ=255)
                                                                             tttCCTGTCGTTGCCTCTTAACTCATTGAAATAGTGTAGTCGGCGTCAAAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgtgt                                                                       <  1:174162/149‑1 (MQ=255)
                                                                                  cgTCTTTGACTCTACTCTCATTGAAATCGTGTAGTCGGCGTCACAAAAAGGTGAGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGACAAGCCATGAAGTGGCAACAACGTGTTCgtg                                                                  <  1:1304835/148‑1 (MQ=255)
                                                                                    tCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                                                                >  1:1194742/1‑149 (MQ=255)
                                                                                    tCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                                                                >  2:977209/1‑149 (MQ=255)
                                                                                      tttGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTctc                                                              >  1:713318/1‑147 (MQ=255)
                                                                                          ccTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGTAGAGAAGCCATGAAGTGGCAACAACCTGTTCGTGTCGCAACg                                                          >  2:694455/1‑149 (MQ=255)
                                                                                          ccTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGACATGAAGTGGCAACAACGTGTTCGTGTCGCAACg                                                          >  1:332652/1‑149 (MQ=255)
                                                                                            tctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTACAGGAGAGAAGCCATTAAGTGGCTACAACGTGTTCGTGTCGCAACggg                                                        >  1:401622/1‑149 (MQ=255)
                                                                                             ctTATCTCTTTGAAAAAGTGTTGTCGGCGGCACAAAAAGGTGCGGGATTACGGAAGTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGGAACGGGt                                                       <  1:694455/149‑1 (MQ=255)
                                                                                                   tcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATATATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCTTGTCGCATCGGGTCTAAGt                                                 >  1:122223/1‑149 (MQ=255)
                                                                                                    cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGtt                                                <  1:82465/149‑1 (MQ=255)
                                                                                                     aTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTg                                               >  2:1130131/1‑149 (MQ=255)
                                                                                                       tGAAATAGTGTCGGCGGCGTCACAAACAGGGGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt                                             <  1:447429/149‑1 (MQ=255)
                                                                                                       tGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCt                                             <  1:710358/149‑1 (MQ=255)
                                                                                                        taaaTAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTATAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTg                                            <  1:453967/148‑1 (MQ=255)
                                                                                                          aaTAGTGTAGTCGGCGTCACAAAAAGGTGCTGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGc                                          <  1:647368/149‑1 (MQ=255)
                                                                                                                gtAGTCGGCGTCAAAAAAAGGTGCGGTCTTACTGAATTTGCCGTAAAGTTCGGTACTCTGAGTTAGTAGAGATAAATTCTTCAGGAGAGTAGCCATGAAGTTGCTACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTa                                    <  1:1017474/149‑1 (MQ=255)
                                                                                                                 tAGTCGGCGTCACAAAAATGTGAGGTCTTACGGAATTTTCCGTTAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTGAGGAGAGAAGCCATTACTTGGCTACAAAGTGTTAGAGTCGAAACGGGTCTAAGAAGCTGGCAGAtttt                                   >  2:1045422/1‑147 (MQ=255)
                                                                                                                   gTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGt                                 >  1:896803/1‑149 (MQ=255)
                                                                                                                       gCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCa                             <  1:281811/149‑1 (MQ=255)
                                                                                                                       gCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCa                             >  1:145412/1‑149 (MQ=255)
                                                                                                                            acaAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTAc                        >  1:439842/1‑149 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      >  1:184917/1‑149 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      >  2:1218264/1‑149 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      >  2:599938/1‑149 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      <  1:1378462/149‑1 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGATCGGTACTCTGAGTAAGTAGAGAAAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTACGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      >  2:466584/1‑149 (MQ=255)
                                                                                                                              aaaaaGGTGAGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCTTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                      <  1:1269063/149‑1 (MQ=255)
                                                                                                                                aaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGt                    <  1:1374254/149‑1 (MQ=255)
                                                                                                                                   ggTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGt                 <  1:599938/149‑1 (MQ=255)
                                                                                                                                   ggTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGt                 <  1:1218264/149‑1 (MQ=255)
                                                                                                                                    gTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCGGCGTAAGTAGAGATATATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGGAGTTACTGGAAGTg                >  1:1190074/1‑149 (MQ=255)
                                                                                                                                      gCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGgc              >  1:1215391/1‑149 (MQ=255)
                                                                                                                                         actttACGGATAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGACATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCgct           >  1:905855/4‑149 (MQ=255)
                                                                                                                                           tttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGCAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCACCGGGTCTAAGTAGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCcctca         >  2:480502/2‑147 (MQ=255)
                                                                                                                                                ggAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtg    <  1:167006/149‑1 (MQ=255)
                                                                                                                                                  aaTTTTCCGTTAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTGCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtggt  <  1:1569169/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGT  >  NC_000913/824424‑824718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: