Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,578 C→G 5.7% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5780CG 5.7% 307.9 / 7.6 111intergenic (+23/+218)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base C (58/45);  new base G (4/3);  total (63/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 113 total aligned reads displayed.

TTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGC  >  NC_000913/857430‑857726
                                                                                                                                                    |                                                                                                                                                    
tttGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAGCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGAGTATCCGTCACCCCGATAc                                                                                                                                                      >  1:536785/1‑149 (MQ=255)
      tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCCGAACGCGTGATCGACTTAAGCGGTAATTACCATGATTACCTCCGTAGAAAAGGGATCGAGTAAGTGATTATCCGACACCCCGATACTGTCgg                                                                                                                                                >  1:1566305/1‑149 (MQ=255)
       cactCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACTAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCggg                                                                                                                                               <  1:851191/149‑1 (MQ=255)
       cactCGACCGTGAGTTCGTAAGCTCCCGGGCGACCCGCATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                               <  1:339997/149‑1 (MQ=255)
       cacaCGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                               <  1:28991/149‑1 (MQ=255)
        acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                              >  2:90249/1‑149 (MQ=255)
        acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                              >  1:186266/1‑149 (MQ=255)
        acacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgggg                                                                                                                                              >  1:338714/1‑149 (MQ=255)
         cacGACCGTGTGTTCGTAAGCTCCCTGGCGACCCGCATTCTTGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGt                                                                                                                                             <  2:915787/149‑1 (MQ=255)
          acGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTTGTAAAGGGATAGAGTAAGTGATTATCCGTCACCCCGAAACAGTCGGGGTg                                                                                                                                            >  2:729691/1‑149 (MQ=255)
            gACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAc                                                                                                                                          <  2:513538/149‑1 (MQ=255)
              ccGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcgg                                                                                                                                        >  1:791898/1‑149 (MQ=255)
               cGTGAGTTCGTAAGCTCCGTGGCGACCCGCATTCTTGAAATCAGCCCGGAACGCGTGATCTAATTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATTGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggc                                                                                                                                       <  2:1116406/149‑1 (MQ=255)
                gTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTATAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcg                                                                                                                                      >  1:1408482/1‑149 (MQ=255)
                 ggtgTTCTTAAGCTCCCTGGCGACCCGCATGCTGCAAACCCCCCCGCAACGCGTGACCGACTTTCGCGGTAATTCCGAACATTACCTGCGTATTAACGGGAGCGCGAAAGTGATTACCCGTCACCCCCATACTGGGGGGGGGAcggcgg                                                                                                                                     <  2:606989/146‑1 (MQ=255)
                  gAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGACGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                                    <  1:549651/149‑1 (MQ=255)
                     ttCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                                 >  1:86064/1‑149 (MQ=255)
                     ttCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                                 >  1:1230474/1‑149 (MQ=255)
                       cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatg                                                                                                                               >  1:1518693/1‑149 (MQ=255)
                         tAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGAc                                                                                                                             >  2:896845/1‑149 (MQ=255)
                         tAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAACGACTTTAGCGGTAATTACGATGATTAACTGCGTAGTAAAGGGATCGAGTATGTGATTATCCGTCACCCCGATAATGGAGTGGTGACGGCGTGTGATGAc                                                                                                                             >  1:1469038/1‑149 (MQ=255)
                            gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGCCTTTAGCGGTAATTACGTAGATTACCTGCGTAGTACAGGGATCGAGTAAGTGATTATCCGTCACCACGCTACTGACGGGGGGCCGGCGGCtgatgtcgta                                                                                                                          >  2:1015641/1‑144 (MQ=255)
                            gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACaaa                                                                                                                          >  1:1467299/1‑149 (MQ=255)
                            gCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAGCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGACTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACaaa                                                                                                                          <  1:86230/149‑1 (MQ=255)
                            gCTCCCTGGCGACCCGCAATCTGGAAATCCCCCCGGAACGCGGGATCGACTTACGCGGTAATTACGAAGATTACCTCCGAAGTAAAGGGATCGAGAAAGTGATTACCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACaaa                                                                                                                          <  1:445108/149‑1 (MQ=255)
                             cTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAt                                                                                                                         <  2:1097512/149‑1 (MQ=255)
                                ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAATATTCCCTGCGTAGTAAAGGGATCGAGTAAGTGATTACCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                      <  1:712008/149‑1 (MQ=255)
                                ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                      >  2:678760/1‑149 (MQ=255)
                                ccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATACGTCACCCCGATACTGTCGTGGTGACGGCGGCTGATGACAAATGCa                                                                                                                      >  2:435820/1‑149 (MQ=255)
                                       accgcctttCTGGAAATCACCCCGGAACGCGTGATCGCCTTTAGCGGTAATTACGACGATTACCTGCGTAGTAAAGGGATCGCGTAAGGTATTATCCGTCACCCCGATACTTTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGc                                                                                                               <  1:337035/142‑1 (MQ=255)
                                         ccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                             >  1:1226638/1‑149 (MQ=255)
                                            cATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTTGCGGTAATTACGATGATTACCTGCGTAGTAAAGGGATGGTGTAAGTGATTATCCGTCACCCCGATACTTTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGAt                                                                                                          <  1:160501/149‑1 (MQ=255)
                                                ttGGAAATCACCCGGTAACTCGTGATGGACTTTAGCGTTAATTACGATGATTACGTGTGTAGTAAAGGTATCGAGAAAGTGATTATCGGTCACCCCGAACGTGGCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATgcgc                                                                                                      <  1:1187523/148‑1 (MQ=255)
                                                cTGTAATTCACCCCGGAACGCGTGATCGACTTTAGCCGTAATTACGAAGATTACCTGCCTACTAAAGGGATCTAGTAATTGATTATCCGTCACCCCCATACTGTCGGGGAGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgcgc                                                                                                      <  2:103488/149‑1 (MQ=255)
                                                  ggAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGACTGATGCGCTa                                                                                                    >  1:91410/1‑149 (MQ=255)
                                                    aaaTCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGCTACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACg                                                                                                  >  1:524303/1‑149 (MQ=255)
                                                       tcccccgGGAACGCGGCATCGCCTTTCGCGGTAATACCGAAGATTCCCGGCGAAGTAAAGGGATCCAGAAAGTGATAATCCGTCCCCCCCATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCtt                                                                                               <  2:193667/146‑1 (MQ=255)
                                                         acgccGGAACGCTTTATCGACTTTATCGTTAATTACTAATATTACCTGGGTAGAAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGTTACTGTCGGGGTGACGGCGGCTGATGACAAATGCTAAATAGCCTGATGCGCTACGCTTAt                                                                                             <  2:1168691/146‑1 (MQ=255)
                                                         aCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCTTAGTAAAGGGATCGAGTATGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTAt                                                                                             <  2:92634/149‑1 (MQ=255)
                                                          ccccGGAACGCGGGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATc                                                                                            <  2:689852/149‑1 (MQ=255)
                                                           cccGCACCGCGTCATCCACTTACGCGGTAAATACGACGATTCCCTGCGTCGTAACGGGCTCGCGTACGTGATTAACCGGCCCCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACTCTTATCa                                                                                           <  1:1523246/149‑1 (MQ=255)
                                                              ggAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGc                                                                                        <  1:90249/149‑1 (MQ=255)
                                                               gAACGGGTGATCGACTTTAGCGGAATTTACGAAGATTACCTGCGTAGTAATGGGATCGATTAAGTGATAATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGcc                                                                                       <  2:523873/149‑1 (MQ=38)
                                                                   gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                                   >  2:427387/1‑149 (MQ=255)
                                                                    cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                                  >  2:1278328/1‑149 (MQ=255)
                                                                    cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGATGATGACAAATGCAAAATAACCTGATGCGCTACGCTTATCAGGCCTACGc                                                                                  >  2:1278332/1‑149 (MQ=255)
                                                                     gTGATCGACTTTAGCGGTAATTACGAAGATTACCTTCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCa                                                                                 >  2:1150873/1‑149 (MQ=255)
                                                                     gTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCa                                                                                 >  2:432244/1‑149 (MQ=255)
                                                                         acGACTTTAGCGGAAATTACGAATATTACCTGGGTAGTAAAGGGACCCAGTAAGTGATTAACCGGCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTAc                                                                             <  1:350806/148‑1 (MQ=38)
                                                                          cGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTAcc                                                                            <  2:220247/149‑1 (MQ=255)
                                                                             atttATCTGTATTTACGAAGATTTCCTGCGTAGTATAGGGATCGAGTAAGTGATTATCCTTCAGCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAATATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGc                                                                         <  2:1566305/148‑1 (MQ=38)
                                                                                tAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAat                                                                      <  2:673122/149‑1 (MQ=255)
                                                                                  gCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatat                                                                    >  1:1394099/1‑149 (MQ=255)
                                                                                   cGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAAGGCGGCTGATGACAAATGCAAAATAGCCTGCTGCGCTACGCTTATCAGGCCTACGCAGTCCCTGCAatata                                                                   >  2:1194890/1‑149 (MQ=255)
                                                                                     gTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                                 >  2:1331552/1‑149 (MQ=255)
                                                                                         ttACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGTGTGACGGAGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGATTATCAGGCCTACGCAGTACATGCAATATATTGAAt                                                             >  2:89700/1‑149 (MQ=255)
                                                                                          tACGAATATTACCTGCTTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATc                                                            <  1:1374334/149‑1 (MQ=255)
                                                                                          tACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATc                                                            >  2:510828/1‑149 (MQ=255)
                                                                                            cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATATg                                                          >  2:855013/1‑149 (MQ=255)
                                                                                            cGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCAACCAGATACTGTCGGGGTGACGGCGGCTGATGACAAATCCAAAATAGCCTGATGCCCTACGCTAAACAGCCCTACGCAGTACCTGAAATAAATTGAATcaa                                                          >  1:583541/1‑147 (MQ=255)
                                                                                              aaGATTACCTGCGTAGTAAAGTGATCGAGAAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTTATGACAAATTCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCa                                                        <  2:1316067/149‑1 (MQ=255)
                                                                                              aaGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCa                                                        <  2:375768/149‑1 (MQ=255)
                                                                                                 aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCCCCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGa                                                     <  1:445399/149‑1 (MQ=255)
                                                                                                    aCCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGTCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACtt                                                  >  1:945691/1‑149 (MQ=255)
                                                                                                     ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCGGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACttt                                                 <  1:87221/149‑1 (MQ=255)
                                                                                                        gCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGAAGGACTTTGTa                                              >  1:1179660/1‑149 (MQ=255)
                                                                                                           tagtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGTTACTGTCGGGGTGACGGAGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGc                                           <  1:435820/149‑1 (MQ=255)
                                                                                                            agtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGcc                                          <  2:1230474/149‑1 (MQ=255)
                                                                                                            agtaAAGGGATCGAGTAAGTGATAATCCGTCACCCCGATCCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGcc                                          <  1:692753/149‑1 (MQ=255)
                                                                                                              taAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                        >  2:337709/1‑149 (MQ=255)
                                                                                                              taAAGGGATCGAGTAAGTGATTATCCGTAACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                        <  2:338714/149‑1 (MQ=255)
                                                                                                               aaaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGa                                       >  1:839516/1‑149 (MQ=255)
                                                                                                                aaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCTGGACTTTGTAGGCCGGAt                                      >  2:797844/1‑149 (MQ=255)
                                                                                                                aaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                      >  2:17190/1‑149 (MQ=255)
                                                                                                                aaGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGAt                                      >  1:1503661/1‑149 (MQ=255)
                                                                                                                 aGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                     >  1:1371562/1‑149 (MQ=255)
                                                                                                                   ggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                                   >  1:413158/1‑149 (MQ=255)
                                                                                                                   ggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                                   >  1:1263995/1‑149 (MQ=255)
                                                                                                                   gcaTCGAGTAAGTGATTATCCGTCACCCCGATAGTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAg                                   <  2:75603/147‑1 (MQ=255)
                                                                                                                    gATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                                  <  2:186266/149‑1 (MQ=255)
                                                                                                                       cGAGTAACTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGt                               <  1:1445189/149‑1 (MQ=255)
                                                                                                                             aGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGACGGATAAGGCGTTAAcgc                         >  2:341604/1‑149 (MQ=255)
                                                                                                                             aGTGATTAACCGGCACCCCGATCCTGGCGGGGTGACGGCGGCAGATGACAACAGCAAAAAAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgc                         <  1:241584/149‑1 (MQ=25)
                                                                                                                              gTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                        >  2:727307/1‑149 (MQ=255)
                                                                                                                                    aTCCGTCACCCCGATACTGTGGGGGTGACGGCGGCTGATGACAAATGCTAAATAGCCTGATGCGCTACGCTTATCAGGCGTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTTGGCCGGATAAGGCGTTAACGCCGCATCa                  <  2:811759/149‑1 (MQ=255)
                                                                                                                                     tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg                 >  1:219451/1‑149 (MQ=255)
                                                                                                                                     tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTCCAATATTATGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg                 >  2:1307073/1‑149 (MQ=255)
                                                                                                                                     tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATAAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTGAACGCCGCATCAg                 >  1:845991/1‑149 (MQ=255)
                                                                                                                                      ccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg                >  1:958335/1‑149 (MQ=255)
                                                                                                                                       cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc               >  2:332795/1‑149 (MQ=255)
                                                                                                                                        gTCCCCCCGATACTGGCGGGGTGACGGCGGCTGTTTAGAAATGTAATATAGGCTGATGCGCTACGCTTATCAGGCCTTCGCAGTACCTGCAATAAATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa              <  2:524303/149‑1 (MQ=21)
                                                                                                                                        gTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa              >  1:344086/1‑149 (MQ=255)
                                                                                                                                         tCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt             >  1:236598/1‑149 (MQ=255)
                                                                                                                                         tCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt             >  2:267709/1‑149 (MQ=255)
                                                                                                                                          cACCCCGATCCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAtt            <  1:1194134/149‑1 (MQ=255)
                                                                                                                                          cACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAtt            <  1:29968/149‑1 (MQ=255)
                                                                                                                                            ccccTATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACTCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTaa          <  2:1297144/149‑1 (MQ=255)
                                                                                                                                             cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTaaa         >  1:424585/1‑148 (MQ=255)
                                                                                                                                             cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc         >  2:1509368/1‑149 (MQ=255)
                                                                                                                                                    cTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAgcgc  <  2:1209854/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAGCGC  >  NC_000913/857430‑857726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: