| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 125,820 | C→T | 8.1% | A42A (GCC→GCT) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,820 | 0 | C | T | 8.1% | 228.3 / 8.2 | 88 | A42A (GCC→GCT) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base C (37/43); new base T (4/3); total (42/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAG > NC_000913/125673‑125967 | tGGCGTTAGAGGTAAAAGAATAATGGCGATCGAAATCAAAGTACCGGACCTCGGGGCGGATGTAGGTGAAATACCCGACATCCTGGTCAAAGAGGGCGACAAAGTTGAAGCCCTACAGTCGCTGATCACCGTAGCAGGCGACAAAGccg > 2:477824/1‑148 (MQ=255)tGGCGTAAGAGGTAAACGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAACTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCct < 1:354392/149‑1 (MQ=255) ggCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGGTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCTACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCctc < 2:1487920/149‑1 (MQ=255) ggCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCctc > 2:1536897/1‑149 (MQ=255) gTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCCAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTAt > 2:1221465/1‑149 (MQ=255) gagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGaa < 2:1097238/149‑1 (MQ=255) gagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGaa < 2:605713/149‑1 (MQ=255) gagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGaa < 1:225401/149‑1 (MQ=255) ggTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGt > 1:191766/1‑149 (MQ=255) tAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTc > 2:60455/1‑149 (MQ=255) tAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTc > 2:267390/1‑149 (MQ=255) tAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTc > 1:216106/1‑149 (MQ=255) aaaaGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTcc > 2:25355/1‑149 (MQ=255) aaaGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGCCAAAGTTGAAGCCGAACAGGCGCTGATCACCGTAGAAGGCGACAACGCCTCTATGGAAGTACCg > 2:876852/1‑149 (MQ=255) aaGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGt < 2:1433709/149‑1 (MQ=255) taatGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCg < 1:242507/149‑1 (MQ=255) aagggCTATCGAACTCAAAGTACAGGACATCGGGGCTGATTATCTTGAAATCACCGAGATCCAGGTCAAAGTGCGCGACAAAGTTTAACACGAACAGTCGCTGATCACCGTAGACGGCGACAAAGCCGCTATGTAAGTTCCGTCTCCGc < 2:1352184/146‑1 (MQ=25) tATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCgg < 2:1117436/149‑1 (MQ=255) cagcGAAATCAAAGTACCGGACCTCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCgg < 2:227365/146‑1 (MQ=255) gAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTAt > 2:187399/1‑149 (MQ=255) aaaTCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCCGGCGGGTATc > 2:295016/1‑149 (MQ=255) cAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTa > 2:1524100/1‑149 (MQ=255) aaaGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTaa > 2:193384/1‑149 (MQ=255) aaGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTaat < 1:267390/149‑2 (MQ=255) aaGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTaaa < 2:144564/149‑1 (MQ=255) aaGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGATGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTAAGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTaaa > 1:978555/1‑149 (MQ=255) aCCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGATCAGAAGGCTGGGATCGTTAAagata > 2:1329344/1‑147 (MQ=21) aCCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAgaga > 2:87253/1‑149 (MQ=255) ccGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGCCAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGAt > 2:855585/1‑149 (MQ=255) cGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCg < 2:357231/138‑1 (MQ=255) cGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCg > 1:357231/1‑138 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCaaa < 1:564448/149‑1 (MQ=255) aCATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCaaa < 2:464431/149‑1 (MQ=255) gCTTATGAAGGTGAACTCACCGAGCTCCTGGTCAAAGTGGGAGACAAAGTTGAAGCCGAACAGGCGCTGATCACCGTAGAAGGCGACAAAGCCGCTAGGGAAGTGCCGGCTCCGCAGGCGGGTATCGTTAACTAGATCAAAGTCTCTGt < 2:1122928/149‑1 (MQ=21) gCTGATGAAGTTGAACTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGt < 2:1310800/149‑1 (MQ=255) tgatgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTg > 2:1220149/1‑149 (MQ=255) gatgaAGTTGAAATCACCGAGATCCTGGGCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGCTGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTgg < 1:1121487/149‑1 (MQ=255) atgaAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa > 2:1107055/1‑97 (MQ=255) atgaAGTTGAAATCACCGACATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa < 1:1107055/97‑1 (MQ=255) aggtGAAATCACCGAGATCCTGGTCAAAGTGGTCGACAATGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCTACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATa < 2:1046514/146‑1 (MQ=255) ttGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATaaa > 2:24916/1‑149 (MQ=255) gAAATCCCCGAGATCCTGGTCAAAGTGGGCGACAAAGGTGAAGCGGCTCAGGCGCTGATCACCGTAGCAGGCGACAAGGCGTCGATGGAAGTTCCGGCTCGGGTGGCGGGGAGCGGGAAAGAGAGCCAAGTGGCGGTTGGTGACaaggg > 2:719983/1‑146 (MQ=255) gAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGCTCAAAGTCTCTGTTGGCGATAAAAc > 2:664976/1‑149 (MQ=255) gAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAc > 1:542538/1‑149 (MQ=255) aaaTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAAATTCGCTGATCACCGTAGAAGGCGACTAAGGCTCTATGGAAGATCCTTCTCCGCAGGAGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGGTatcacc > 1:1400643/1‑144 (MQ=25) aaTCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAAccc < 1:87253/149‑1 (MQ=255) cACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCGGATCACCGTAGAAGGCGACTAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGTGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGa < 2:1213685/149‑1 (MQ=255) ccGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGCGCTCAACGTCTCGGGTGGCGAGAAACCCCCGAcc > 1:1571568/1‑149 (MQ=21) cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa < 2:732118/82‑1 (MQ=255) cGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGaa > 1:732118/1‑82 (MQ=255) agttCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCGGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgc < 1:1207038/146‑1 (MQ=255) agaTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgc > 2:48378/1‑149 (MQ=255) gaTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCTTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGTTCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgcg < 1:90967/149‑1 (MQ=255) aTCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGAGCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGgcgc < 2:1057413/149‑1 (MQ=255) ccTGGGCACAGTGGGCGACAAAGTTGAAGCCGAACAGGCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCAc < 2:873290/149‑1 (MQ=255) ccTCGTCAAAGTGGGCGACAAAGTTGAACCCGAACAGTCGCTGATCACCGTAGAAGTCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCAc < 2:1276498/149‑1 (MQ=255) cTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGCCCGGCGCACt > 2:739803/1‑149 (MQ=255) cTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACt > 2:138381/1‑149 (MQ=255) aGTGGGCGACAAAGTTTAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGGCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGa < 1:477824/149‑1 (MQ=255) aGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg < 1:73215/74‑2 (MQ=255) aGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg > 2:73215/1‑73 (MQ=255) gCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTc < 2:216106/149‑1 (MQ=255) gACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACGGGCGCACTGATGATGATTTTCGa > 1:542420/1‑149 (MQ=255) gTTGAAGCCGAACTGTCGCTGATCTCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTATAGAGATCATATTCTCTGTTGTGGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTccgc < 2:1365313/149‑1 (MQ=255) gTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAATGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTccgc > 2:1154549/1‑149 (MQ=255) ttGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTccgcc < 1:1221465/149‑1 (MQ=255) cGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTg < 1:734506/149‑1 (MQ=255) aaCAGTCGCTGATCAACGTAGAACGCTACAAACCCTCTATGGAACTACCGTCTCCGCAGGCGGGTATCGTTAAAGAGATGAAAGTCTCTGATGGCGATAAAACCCAGACCGTCGCAATGATGATGATTTTCGATTCCGCCGACGGTgca > 1:300286/1‑149 (MQ=255) aGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGAAAAAACCCAGACCCGCGCACTGATTATGATTTTAGATTCCTCCGACGGTgcagca > 2:1018705/1‑149 (MQ=255) gCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACg > 2:561632/1‑149 (MQ=255) gCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACg > 2:1174765/1‑149 (MQ=255) gCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACg < 2:191766/149‑1 (MQ=255) gCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACg > 1:467665/1‑149 (MQ=255) cTGATCACCGTAGAAGTCGAAAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGTTCAAAGTCTCTGTTGTCGATACAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGc < 2:907266/149‑1 (MQ=255) aTCACCGGAGAAGGCGACAAAGCCTCTATGGCAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGc < 2:429634/149‑1 (MQ=255) tCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGAATTTCGATTCCGCCGACGGTGCAGCAGACGCTGCa < 1:380057/149‑1 (MQ=255) aCCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCACTGTTGGCGATAAAACCCAGACCCGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAcc > 1:1303324/1‑149 (MQ=255) cGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTg > 1:101512/1‑149 (MQ=255) gTAGAAGGCTAAAAAGCCTCTATGCAAGTTCCGTCTCCGCAGTCGTTTATCGTTAAAGAGATCAAAGTCTCTTTTGGCGATAAAACCCAGACCGGCGCACTGATTATGAATTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGc < 1:876852/149‑1 (MQ=255) aGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGCCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTc < 1:295016/149‑1 (MQ=255) aaGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAg < 1:1495489/149‑1 (MQ=255) aaGGCGACAAAGCCGCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTCAAGCGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTACGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAg < 1:129982/149‑1 (MQ=255) aGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAgg > 2:1213011/1‑149 (MQ=255) ggCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGc < 1:932896/149‑1 (MQ=255) gACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATAATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCaga < 1:138381/149‑1 (MQ=255) gACAAAGCCTCTATGGAAGATCCGTCTCCGCATGCGGGAATCGTTTAAGAGAACAAAGTCTCTCTTGCCGATAACACCCAGAACGGCGCACTTATTATGATTTTCGATTCCGCCGACCGTGCAGCAGACGCTGCCCCTGCTGAGGCaga > 1:1518707/1‑149 (MQ=255) cAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaag < 2:542538/149‑1 (MQ=255) aaaGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaaga > 2:583996/1‑149 (MQ=255) aGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaagaga < 1:48378/149‑1 (MQ=255) gCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAACGTCTCTGTTGGCTCTAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaagagaa < 2:488673/149‑1 (MQ=255) gCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAc > 1:1259306/1‑130 (MQ=255) gCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCAc < 2:1259306/130‑1 (MQ=255) ccTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGGCGCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCagaagagaag < 1:187399/149‑1 (MQ=255) | TGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAG > NC_000913/125673‑125967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |