Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,030,081	A→G	6.0%	I6M (ATA→ATG)	yccX →	weak acylphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,030,081	0	A	G	6.0%	183.5 / 3.0	67	I6M (ATA→ATG)	yccX	weak acylphosphatase
Reads supporting (aligned to +/- strand):  ref base A (34/29);  new base G (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

CCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGT  >  NC_000913/1029940‑1030227                                                                                                                                              |                                                                                                                                                   cccTTTGGCTAACACTAAACGTACCCTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCAGTAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCt                                                                                                                                             >  2:729379/1‑149 (MQ=255)  ccTTTGGCTAACACTAAACGTACACTCATACTTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATGATTGc                                                                                                                                               <  2:1456867/146‑1 (MQ=255)  ccTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATGATTGc                                                                                                                                               >  1:1451545/1‑146 (MQ=255)  ccTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATGATTGc                                                                                                                                               >  1:1456867/1‑146 (MQ=255)  ccTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATGATTGc                                                                                                                                               <  2:1451545/146‑1 (MQ=255)   cTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTgg                                                                                                                                           <  2:1287901/149‑1 (MQ=255)         cTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGTAAGTCTGCATAATTGCCTGGGTATAc                                                                                                                                     >  2:932228/1‑149 (MQ=255)               cTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGa                                                                                                                               >  1:793211/1‑149 (MQ=255)                tAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCCACGCGTCCATTAAGGATAAGGAGAGCCGCAATGTCGACAGTCTGCCTAATTGCCTGGGTTTACGGGCGAg                                                                                                                              >  1:859922/1‑149 (MQ=255)                  aaCGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGtt                                                                                                                            >  2:897965/1‑149 (MQ=255)                  aaCGTACACTCATAATTTACTTTTCGGTCGATGCAAAGCAATGGGCGTCCTTGTCCGGCGTGTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCATCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTCCGGGCGAGtt                                                                                                                            >  2:1137621/1‑149 (MQ=255)                   aCGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTc                                                                                                                           >  2:364205/1‑149 (MQ=255)                   aCGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTc                                                                                                                           >  2:576181/1‑149 (MQ=255)                     gTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCAGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAAGGTCGAAAGTCTGCATAATTCCCTGGGTTTTCGGGCGAGTTCAg                                                                                                                         >  2:1540078/1‑149 (MQ=255)                      tACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAgg                                                                                                                        <  2:230620/149‑1 (MQ=255)                        cacTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGc                                                                                                                      <  1:303309/149‑1 (MQ=255)                                   tACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGTATTTGCAACGCGTCCATTAAGGATAAGGAGAGAAGCAATGTCGAAAGTCTGCATAATTGCCTGTGTTTACGTGAGAGTTAAGGTGGTAGGATTTCg                                                                                                           >  2:411314/1‑149 (MQ=255)                                   tACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCg                                                                                                           <  2:114109/149‑1 (MQ=255)                                      ttttCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa                                                                                                        <  2:38709/149‑1 (MQ=255)                                         tCTGTCGATGCAACGAAATGGGCGTCATTGTCCGGAGGTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTacac                                                                                                     <  1:1320943/149‑1 (MQ=255)                                         tCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTacac                                                                                                     <  1:624323/149‑1 (MQ=255)                                         tCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTacac                                                                                                     <  1:11254/149‑1 (MQ=255)                                           tGTCGATGCAAAGAAAGGGGCGGCATTGGCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca                                                                                                   <  1:282807/149‑1 (MQ=255)                                             tCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACcaca                                                                                                 >  2:1344709/1‑149 (MQ=255)                                              cGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACcacac                                                                                                <  1:1119057/149‑1 (MQ=255)                                                 tGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGt                                                                                             <  1:894080/149‑1 (MQ=255)                                                    aaaGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTTCg                                                                                          >  2:1561524/1‑149 (MQ=255)                                                    aaaGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACg                                                                                          >  1:800467/1‑149 (MQ=255)                                                     aaGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGAGTTGCAACGCGTCCAGTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACGCAACACAGTACGa                                                                                         >  1:1409250/1‑149 (MQ=255)                                                       gAAATGGGCGGCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAg                                                                                       <  1:906080/149‑1 (MQ=255)                                                       gAAATGGCCGTCATAGTCCGGAGCTTTCCATGGATTTGCAACGCGTCCATTAATGATAAGGAGAGCAGCAATGTCTAAAGTCAGCATAATTGCCTGGCTTTACGGGCGAGTTCAGGGCGTATGATTTCGCTACACCACACAGTACGAAg                                                                                       <  1:411314/149‑1 (MQ=255)                                                             ggCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGaaaa                                                                                 <  2:596948/149‑1 (MQ=255)                                                                  cATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTg                                                                            >  1:870209/1‑149 (MQ=255)                                                                  cATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTg                                                                            >  1:687960/1‑149 (MQ=255)                                                                     tGTCCGGATTTTTCCATTGATTTGCAACGCGTCCATTAATGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGt                                                                         <  2:1418928/149‑1 (MQ=255)                                                                      gTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGTTAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGtt                                                                        <  2:641004/149‑1 (MQ=255)                                                                       tCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGttt                                                                       >  2:547978/1‑149 (MQ=255)                                                                          gcaGTTTTCCATGGATTTGCAAGGCGTCCTTTAAGGATAAGGAGAGCAGCATTGTCGAAAGTCTGGATAATTACGTGGGTTTTCGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACTCAGTAGGAAGAGAAAAGACTGGGTTTAAc                                                                    >  2:928091/3‑149 (MQ=255)                                                                                ttCCATGGATTTGCAACGCGTCCATTAAGGATAATGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTa                                                              >  1:1076151/1‑149 (MQ=255)                                                                                 tCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCACTGTCGACAGTCTGCATAATTGCCTGGGATTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTGTAACCGGGTAc                                                             <  1:133332/149‑1 (MQ=255)                                                                                 tCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTAc                                                             <  1:617180/149‑1 (MQ=255)                                                                                   cATGGATTTGCAACGCGTCCAGTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGAATTCGCTACACCACACAGTACGAAGCGAAAAGACTGGCTTTAAACGGGTCCGc                                                           >  2:75449/1‑149 (MQ=255)                                                                                     tGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGCCTGGGTTTAACCGGGTACGCCa                                                         >  2:301349/1‑149 (MQ=255)                                                                                      ggATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACATAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaa                                                        >  1:685004/1‑149 (MQ=255)                                                                                           tGCAACGCGGCCATTACGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGCGCGAGATCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATc                                                   <  2:463114/149‑1 (MQ=255)                                                                                            gCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGGTTAACCGGGTACGCCAAAAATCt                                                  >  2:1328705/1‑149 (MQ=255)                                                                                            gCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCGGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCt                                                  <  2:637401/149‑1 (MQ=255)                                                                                             cAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCtt                                                 <  2:280270/149‑1 (MQ=255)                                                                                              aaCGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTg                                                >  1:452399/1‑149 (MQ=255)                                                                                               aCGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGa                                               >  2:9548/1‑149 (MQ=255)                                                                                               aCGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGa                                               >  2:37766/1‑149 (MQ=255)                                                                                               aCGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGa                                               <  1:576181/149‑1 (MQ=255)                                                                                               aCGCGTCCAATAAGGCTAAGGTGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGGTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTTCACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGa                                               <  2:1298821/149‑1 (MQ=255)                                                                                                   gTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCCTAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACTCCACACAGTACGAAGCGAAAAGACTGGGTTTTACCGGGTACGCCAAAAATCTTGACGAt                                           >  2:307392/1‑149 (MQ=255)                                                                                                    gccATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATg                                          <  2:146954/148‑1 (MQ=255)                                                                                                     ccATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTATGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTACCCGGGTACGCCAAAAATCTTGACGATgg                                         >  1:1438793/1‑149 (MQ=255)                                                                                                       aTTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAACTCTTGACGATGGCa                                       >  1:705678/1‑149 (MQ=255)                                                                                                       aTTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACACCAAAAATCTTGACGATGGCa                                       >  1:309540/1‑149 (MQ=255)                                                                                                                   gagaGCAGCAATGTCGAAAGTATGCATAATTGCCGGGGTTTACGGGCGAGTGCAGCGCTTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACAGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTgg                           <  2:859922/149‑1 (MQ=255)                                                                                                                     gagCAGCTATGTCTAAAGTCTGCATATTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGtt                         <  2:793211/149‑1 (MQ=255)                                                                                                                     gagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGACGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGtt                         <  1:287238/149‑1 (MQ=255)                                                                                                                        cagcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGttgct                      >  1:577249/1‑149 (MQ=255)                                                                                                                         agcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGCCTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGttgctt                     >  1:562558/1‑149 (MQ=255)                                                                                                                          gcaATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCACGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTATCAGTGTACGACAAAAATCTTGACGATGGCAGCGTGGAAGTGGttgcttg                    >  2:341872/1‑149 (MQ=255)                                                                                                                              tGTCGAAAGTCTCCAAAATTGCCTGGTTTTCCGGGAGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGt                <  1:113079/149‑1 (MQ=255)                                                                                                                                 cGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTgaa             >  2:50866/1‑149 (MQ=255)                                                                                                                                            aTACTTGCCCGGGTTTACGGGCGAGTTCAGGGCGTAGGACTTCGCTACACCACACAGTTCGAAGCGAAAAGACTGGGTCTACCCGGGTACGCCAAAAATCTCGACGATGTCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGt  <  2:1535462/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   CCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTGCAACGCGTCCATTAAGGATAAGGAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGATGGCAGCGTGGAAGTGGTTGCTTGCGGTGAAGAAGGGCAGGT  >  NC_000913/1029940‑1030227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑