Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,980 G→T 7.6% K120N (AAG→AAT plsX → putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9800GT 7.6% 219.1 / 8.5 93K120N (AAG→AATplsXputative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (40/46);  new base T (2/5);  total (42/51)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-01

ATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTG  >  NC_000913/1147835‑1148128
                                                                                                                                                 |                                                                                                                                                    
aTGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGccc                                                                                                                                                   >  1:77610/1‑149 (MQ=255)
aTGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGccc                                                                                                                                                   >  2:851358/1‑149 (MQ=255)
   cccGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTg                                                                                                                                                >  2:91877/1‑149 (MQ=255)
        ccTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTTGAggg                                                                                                                                           >  2:191117/1‑149 (MQ=255)
        ccTTCGCAAGCTATCCGCGCCAGGCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAggg                                                                                                                                           <  2:503206/149‑1 (MQ=255)
          ttCGCAATCTATCCGCGGCAGTCGTGGGAGTTCACTGCGCGGGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGAAATACAGGGGCGCTGTTGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                         <  2:818760/149‑1 (MQ=255)
          ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                         >  2:1328243/1‑149 (MQ=255)
          ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                         >  1:1289057/1‑149 (MQ=255)
          ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                         >  2:810064/1‑149 (MQ=255)
            cGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCtt                                                                                                                                       <  2:715961/149‑1 (MQ=255)
             ccAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTg                                                                                                                                      <  1:756270/148‑1 (MQ=255)
              cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTGCTCAAGCCCCTGGAGGGGATTGa                                                                                                                                     >  1:1422731/1‑149 (MQ=255)
              cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGa                                                                                                                                     <  1:288039/149‑1 (MQ=255)
               aaGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                    <  1:609779/149‑1 (MQ=255)
               aaGCTATCCGCGCCAGGCGGGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                    <  1:534331/149‑1 (MQ=255)
               aaGCTATCCCCGCCAGTCGTGTGAGTTCACTGCGCTTGGCCCTCGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGGCAGTTCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                    <  2:1002562/149‑1 (MQ=255)
                  cTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGAGGCCCTCGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGTTTGAGCGt                                                                                                                                 >  1:1517887/1‑149 (MQ=255)
                     tCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                                                                                                                              >  2:1074337/1‑149 (MQ=255)
                     tCCGCGCCAGTCATGGGAGTTCAATGCGCGTGGCCCTGGAGCTTGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGGGCGGATTGGGCTGGGAAAATTTTATCTCAAGCCAGTGGAGCGGATTGAGAGACCg                                                                                                                              >  2:852894/1‑149 (MQ=255)
                      ccGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGGGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCgg                                                                                                                             <  2:444511/149‑1 (MQ=255)
                         cgcCAGTCGTGGGAGTTCAAAGCGCGTGGCCCTGGAGCGGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgcgc                                                                                                                          <  2:1126974/149‑1 (MQ=255)
                         cgcCACCCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGATAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgcgc                                                                                                                          <  1:1131774/149‑1 (MQ=255)
                                 gTGGGAGTTCAATGCGCGGGGCCCTGGAGCTGGTGAAAGACGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTTGCAAAATTATTCCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACg                                                                                                                  <  1:1042086/149‑1 (MQ=255)
                                  tGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAATGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                 <  2:200864/149‑1 (MQ=255)
                                  tGGGAGGTCAATGCGCGGGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                 <  1:698467/149‑1 (MQ=255)
                                  ggggAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                 <  1:698470/148‑1 (MQ=255)
                                    ggAGTTCAATGCGCGTGGCCCGGGAGCTGGGGAAAGAAGGTCGAGGGCAAGCCTGTGTCAGTGCCGGTAATAGCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTa                                                                                                               <  2:1414589/149‑1 (MQ=255)
                                       gTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGACGGTAATACCGGGGCGATGATGGGGCTGGCAAAATTATTACACAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                            >  2:952572/1‑149 (MQ=255)
                                             tGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGTGGGGATGGAGCTTCCGGCGCTGGTGACGTTATTACcacaa                                                                                                      >  2:347290/1‑148 (MQ=255)
                                              gcgcGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                     >  1:1249918/1‑149 (MQ=255)
                                              gcgcGTGGCACTGGAGCTGGTGAAAGAAGGTCGAGCGCAACCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGTGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGTTTGAGCGTCCCGCGCTGGTGACGGTATTACcacaaa                                                                                                     >  1:232398/1‑147 (MQ=255)
                                               cgcgTGGCCCTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGCGACGGTATTACCACATCa                                                                                                    <  1:851358/149‑1 (MQ=255)
                                               cgcgTGGCCCTGGAGCTGGTGAAAGAACGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCa                                                                                                    <  1:480322/149‑1 (MQ=255)
                                               agcgTGTCCCTGGATCTGGTGAAAGATGGTCGAGCTCAAGACTGTGTCATTGCCGGTAATTCGGGGGCGCTTATTGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTACGGCGCTGGTTACTGTATTACCACATCa                                                                                                    <  2:1352545/148‑1 (MQ=255)
                                                 cgTGGCCCTGGACCAGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGc                                                                                                  <  2:1459890/149‑1 (MQ=255)
                                                   ggggccTGGAGCTGGTGAACGAAGGTCGAGCGCAAGCCTGTGGGAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCaa                                                                                                <  2:164781/145‑1 (MQ=255)
                                                      cccTGGAGCTGGTGTAAGACGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                             <  2:472275/149‑1 (MQ=255)
                                                        cTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCTCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAggg                                                                                           <  1:382325/149‑1 (MQ=255)
                                                           gAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCaa                                                                                        <  2:77610/149‑1 (MQ=255)
                                                                ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGTGCGCAGATGGGGCTGGCAAAATTATTACTCAAGCACCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGcaaaacggcaaaacgg                                                                                   >  2:128008/1‑149 (MQ=255)
                                                                 gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                  <  1:60596/149‑1 (MQ=255)
                                                                  tGACAGATGGTCGAGCGCAAGCCTGTGTCAGTGCCCGTAATTCCGGGGCGCTGATGGGGCTGGCAAATATATTACTCTAGCGCCTGGAGGGGATTGAGAGTGCTGCGATGGTGACGGTATTACCACATCAACAAAAGGGCAAAACggtg                                                                                 <  2:19582/149‑1 (MQ=255)
                                                                  tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAACAAAAGGGCAAAACggtg                                                                                 <  2:1502023/149‑1 (MQ=255)
                                                                  tGAAAGAAGGTCGAGCGCAAGCCTGTGGCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggtg                                                                                 <  1:1074337/149‑1 (MQ=255)
                                                                    aaaGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGTGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggtggt                                                                               >  2:714865/1‑149 (MQ=255)
                                                                    aaaGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTTACGGTATTACCACATCAGCAAAAGGGCAAAACggtggt                                                                               >  1:318716/1‑149 (MQ=255)
                                                                      agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCTCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCCAAACGGTGGTcc                                                                             >  2:1314546/1‑149 (MQ=255)
                                                                      agaagGGCGAGCTCAAGCCGGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTcc                                                                             <  2:543172/149‑1 (MQ=255)
                                                                        aagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAATTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCtt                                                                           <  2:1228010/149‑1 (MQ=255)
                                                                         agGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                          <  1:1369545/149‑1 (MQ=255)
                                                                          gggcGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGa                                                                         <  2:1562767/146‑1 (MQ=255)
                                                                          ggTCGAGCGCTAGCGTGGGTCAGTGCCGGTATTACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGATGGGATTGAGCGTCCGGCGCTGGTGACTGTATTACAACATCAGCAAAAGGGCAAAACGGTGTTCCTTGa                                                                         <  2:130798/149‑1 (MQ=255)
                                                                          ggTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGa                                                                         <  2:1289057/149‑1 (MQ=255)
                                                                           gTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGAc                                                                        >  1:1359706/1‑149 (MQ=255)
                                                                               aGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                    <  1:395857/149‑1 (MQ=255)
                                                                               aGCGCAAGCCTGTGTCAGGGCCGGTAATACCGGGGGGATGATGGGGCTGGCAAAATTATAACTCAAGCCACTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                    <  2:668510/149‑1 (MQ=255)
                                                                                 cgcAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAggg                                                                  >  1:931352/1‑149 (MQ=255)
                                                                                    aaGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGCCTTAGGGGcc                                                               >  2:84367/1‑149 (MQ=255)
                                                                                      gCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGTAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGGCCTTGACTGAGGGGCCac                                                             >  2:1325471/1‑148 (MQ=255)
                                                                                      gCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCGTTGACTTAGGGGCCaa                                                             >  2:938967/1‑149 (MQ=255)
                                                                                         tgtgTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGt                                                          <  1:1556795/149‑1 (MQ=255)
                                                                                         ggtgtCAGTGCCGGTAATACCGGGGCGCGGATGGGGCGGGCAAAATTCTTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTCGGGGCCAACGt                                                          <  2:1212899/148‑1 (MQ=255)
                                                                                         ggtgtCAGTGCCCGTAATCCCGGGGCGCTGAGGGGGCTGGCAAAAATATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGt                                                          <  1:1528222/148‑1 (MQ=255)
                                                                                          gtgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                         >  1:540953/1‑149 (MQ=255)
                                                                                          gtgtCAGTGCCGGTAATACCGGGGCGCGGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                         <  1:600131/149‑1 (MQ=255)
                                                                                            gtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                       >  1:404798/1‑149 (MQ=255)
                                                                                             tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAt                                                      <  2:553747/149‑1 (MQ=255)
                                                                                             tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAt                                                      >  1:975898/1‑149 (MQ=255)
                                                                                               aGTGCCTGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATAGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                    >  1:902725/1‑149 (MQ=255)
                                                                                                gTGCCGGTAATCCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAGTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgt                                                   <  1:658767/149‑1 (MQ=255)
                                                                                                 tGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgtg                                                  >  1:349956/1‑149 (MQ=255)
                                                                                                  gCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGa                                                 <  2:1239764/149‑1 (MQ=255)
                                                                                                    cGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACa                                               <  1:973490/149‑1 (MQ=255)
                                                                                                           aCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAAt                                        >  1:792222/1‑149 (MQ=255)
                                                                                                             cGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTTCCACATCAGCAAAAGGGCAAAACGGTGGTGCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGc                                      <  1:574806/149‑1 (MQ=255)
                                                                                                               gggCGCTGAAGAGGCTGGCATAATTATTAATAAAGCCACTGGAGGGGATAGAGAGTCCGCAGCTGGTGACGGTATTAACACATCAGCAAAAGGGAAAAACGTTGGTCCATGACTTAGGGTCCAACGTCGATTGTGACAGCACAAtcatg                                    >  2:736614/1‑145 (MQ=255)
                                                                                                                  cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTg                                 >  2:835761/1‑149 (MQ=255)
                                                                                                                   gcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAACGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGc                                >  1:1029567/1‑149 (MQ=255)
                                                                                                                   gcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGAGGTCGTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGc                                >  1:624542/1‑149 (MQ=255)
                                                                                                                       aTGGGGCTGGCAAAATTATAACTCATGCCCCTGGAGTGGATTGAGCTTCCGGCGCTGGTGACGGTATTACCACATCAGCTAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAAtt                            <  2:1424933/149‑1 (MQ=255)
                                                                                                                         ggggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACTTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                          >  1:751138/1‑149 (MQ=255)
                                                                                                                          gggCTGGCAAAATTATTACTCAAGGCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGTCGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGc                         <  2:739729/149‑1 (MQ=255)
                                                                                                                               ggCAAAATTATTACTCAAGCCCCTGGAGGGGAGTGAGCGTCCGGCGCGGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTa                    <  1:810064/149‑1 (MQ=255)
                                                                                                                                gCAAAATTATTACTCAAGCCGCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGGCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                   <  1:1267593/149‑1 (MQ=255)
                                                                                                                                        attaCTCAAGCCCCTGGAGCGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATGGTGTCAGCACAATGCTGGTGCTAGTTGCCATGATGGGCTCAg           >  1:1083504/1‑149 (MQ=255)
                                                                                                                                          taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCGGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGtt         <  2:1107176/149‑1 (MQ=255)
                                                                                                                                            cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGCCTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCt       >  2:1224847/1‑149 (MQ=255)
                                                                                                                                            cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCAGTATGGGCTCAGTTCt       >  2:407631/1‑149 (MQ=255)
                                                                                                                                            cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAATGTGACACCACAATGCTGGTGCAATTTTCCATTATGGGCTCAGTTCt       >  2:634462/1‑149 (MQ=255)
                                                                                                                                             tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCTCAATGCTGGTGCAAGTTGCCATTATGGGCTCAGTTCTg      >  1:457344/1‑149 (MQ=255)
                                                                                                                                             tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg      >  2:1557410/1‑149 (MQ=255)
                                                                                                                                             tCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTACTg      >  1:746441/1‑149 (MQ=255)
                                                                                                                                              aaaGCCCCTGGAGGGGATTGAGCGGCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTgg     <  1:808297/148‑1 (MQ=255)
                                                                                                                                                 gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTg  >  2:263090/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTG  >  NC_000913/1147835‑1148128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: