Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,147,993 A→C 7.6% I125L (ATT→CTT)  plsX → putative phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,147,9930AC 7.6% 232.3 / 5.8 89I125L (ATT→CTT) plsXputative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (34/46);  new base C (2/5);  total (38/51)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

TTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGA  >  NC_000913/1147845‑1148141
                                                                                                                                                    |                                                                                                                                                    
ttCGCAATCTATCCGCGGCAGTCGTGGGAGTTCACTGCGCGGGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGAAATACAGGGGCGCTGTTGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                                      <  2:818760/149‑1 (MQ=255)
ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                                      >  2:810064/1‑149 (MQ=255)
ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                                      >  1:1289057/1‑149 (MQ=255)
ttCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGa                                                                                                                                                      >  2:1328243/1‑149 (MQ=255)
  cGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCtt                                                                                                                                                    <  2:715961/149‑1 (MQ=255)
   ccAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTg                                                                                                                                                   <  1:756270/148‑1 (MQ=255)
    cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTGCTCAAGCCCCTGGAGGGGATTGa                                                                                                                                                  >  1:1422731/1‑149 (MQ=255)
    cAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGa                                                                                                                                                  <  1:288039/149‑1 (MQ=255)
     aaGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                                 <  1:609779/149‑1 (MQ=255)
     aaGCTATCCGCGCCAGGCGGGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                                 <  1:534331/149‑1 (MQ=255)
     aaGCTATCCCCGCCAGTCGTGTGAGTTCACTGCGCTTGGCCCTCGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGGCAGTTCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAATCCCCTCCAGGGGCTTGAg                                                                                                                                                 <  2:1002562/149‑1 (MQ=255)
        cTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGAGGCCCTCGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGTTTGAGCGt                                                                                                                                              >  1:1517887/1‑149 (MQ=255)
           tCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                                                                                                                                           >  2:1074337/1‑149 (MQ=255)
           tCCGCGCCAGTCATGGGAGTTCAATGCGCGTGGCCCTGGAGCTTGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGGGCGGATTGGGCTGGGAAAATTTTATCTCAAGCCAGTGGAGCGGATTGAGAGACCg                                                                                                                                           >  2:852894/1‑149 (MQ=255)
            ccGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGGGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCgg                                                                                                                                          <  2:444511/149‑1 (MQ=255)
               cgcCAGTCGTGGGAGTTCAAAGCGCGTGGCCCTGGAGCGGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgcgc                                                                                                                                       <  2:1126974/149‑1 (MQ=255)
               cgcCACCCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGATAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgcgc                                                                                                                                       <  1:1131774/149‑1 (MQ=255)
                       gTGGGAGTTCAATGCGCGGGGCCCTGGAGCTGGTGAAAGACGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTTGCAAAATTATTCCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACg                                                                                                                               <  1:1042086/149‑1 (MQ=255)
                        tGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAATGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                              <  2:200864/149‑1 (MQ=255)
                        tGGGAGGTCAATGCGCGGGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                              <  1:698467/149‑1 (MQ=255)
                        ggggAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACgg                                                                                                                              <  1:698470/148‑1 (MQ=255)
                          ggAGTTCAATGCGCGTGGCCCGGGAGCTGGGGAAAGAAGGTCGAGGGCAAGCCTGTGTCAGTGCCGGTAATAGCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTa                                                                                                                            <  2:1414589/149‑1 (MQ=255)
                             gTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGACGGTAATACCGGGGCGATGATGGGGCTGGCAAAATTATTACACAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTa                                                                                                                         >  2:952572/1‑149 (MQ=255)
                                   tGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGTGGGGATGGAGCTTCCGGCGCTGGTGACGTTATTACcacaa                                                                                                                   >  2:347290/1‑148 (MQ=255)
                                    gcgcGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATc                                                                                                                  >  1:1249918/1‑149 (MQ=255)
                                    gcgcGTGGCACTGGAGCTGGTGAAAGAAGGTCGAGCGCAACCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGTGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGTTTGAGCGTCCCGCGCTGGTGACGGTATTACcacaaa                                                                                                                  >  1:232398/1‑147 (MQ=255)
                                     cgcgTGGCCCTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGCGACGGTATTACCACATCa                                                                                                                 <  1:851358/149‑1 (MQ=255)
                                     cgcgTGGCCCTGGAGCTGGTGAAAGAACGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCa                                                                                                                 <  1:480322/149‑1 (MQ=255)
                                     agcgTGTCCCTGGATCTGGTGAAAGATGGTCGAGCTCAAGACTGTGTCATTGCCGGTAATTCGGGGGCGCTTATTGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTACGGCGCTGGTTACTGTATTACCACATCa                                                                                                                 <  2:1352545/148‑1 (MQ=255)
                                       cgTGGCCCTGGACCAGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGc                                                                                                               <  2:1459890/149‑1 (MQ=255)
                                         ggggccTGGAGCTGGTGAACGAAGGTCGAGCGCAAGCCTGTGGGAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCaa                                                                                                             <  2:164781/145‑1 (MQ=255)
                                            cccTGGAGCTGGTGTAAGACGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAg                                                                                                          <  2:472275/149‑1 (MQ=255)
                                              cTGGAGCTGGTGAAAGAAGGGCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCTCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAggg                                                                                                        <  1:382325/149‑1 (MQ=255)
                                                 gAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCaa                                                                                                     <  2:77610/149‑1 (MQ=255)
                                                      ggTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGTGCGCAGATGGGGCTGGCAAAATTATTACTCAAGCACCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGcaaaacggcaaaacgg                                                                                                >  2:128008/1‑149 (MQ=255)
                                                       gTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggt                                                                                               <  1:60596/149‑1 (MQ=255)
                                                        tGACAGATGGTCGAGCGCAAGCCTGTGTCAGTGCCCGTAATTCCGGGGCGCTGATGGGGCTGGCAAATATATTACTCTAGCGCCTGGAGGGGATTGAGAGTGCTGCGATGGTGACGGTATTACCACATCAACAAAAGGGCAAAACggtg                                                                                              <  2:19582/149‑1 (MQ=255)
                                                        tGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAACAAAAGGGCAAAACggtg                                                                                              <  2:1502023/149‑1 (MQ=255)
                                                        tGAAAGAAGGTCGAGCGCAAGCCTGTGGCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggtg                                                                                              <  1:1074337/149‑1 (MQ=255)
                                                          aaaGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGTGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACggtggt                                                                                            >  2:714865/1‑149 (MQ=255)
                                                          aaaGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTTACGGTATTACCACATCAGCAAAAGGGCAAAACggtggt                                                                                            >  1:318716/1‑149 (MQ=255)
                                                            agaagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCTCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCCAAACGGTGGTcc                                                                                          >  2:1314546/1‑149 (MQ=255)
                                                            agaagGGCGAGCTCAAGCCGGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTcc                                                                                          <  2:543172/149‑1 (MQ=255)
                                                              aagGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAATTTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCtt                                                                                        <  2:1228010/149‑1 (MQ=255)
                                                               agGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTg                                                                                       <  1:1369545/149‑1 (MQ=255)
                                                                gggcGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGa                                                                                      <  2:1562767/146‑1 (MQ=255)
                                                                ggTCGAGCGCTAGCGTGGGTCAGTGCCGGTATTACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGATGGGATTGAGCGTCCGGCGCTGGTGACTGTATTACAACATCAGCAAAAGGGCAAAACGGTGTTCCTTGa                                                                                      <  2:130798/149‑1 (MQ=255)
                                                                ggTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGa                                                                                      <  2:1289057/149‑1 (MQ=255)
                                                                 gTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGAc                                                                                     >  1:1359706/1‑149 (MQ=255)
                                                                     aGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                                 <  1:395857/149‑1 (MQ=255)
                                                                     aGCGCAAGCCTGTGTCAGGGCCGGTAATACCGGGGGGATGATGGGGCTGGCAAAATTATAACTCAAGCCACTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAg                                                                                 <  2:668510/149‑1 (MQ=255)
                                                                       cgcAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAggg                                                                               >  1:931352/1‑149 (MQ=255)
                                                                          aaGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGCCTTAGGGGcc                                                                            >  2:84367/1‑149 (MQ=255)
                                                                            gCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGTAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGGCCTTGACTGAGGGGCCac                                                                          >  2:1325471/1‑148 (MQ=255)
                                                                            gCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCGTTGACTTAGGGGCCaa                                                                          >  2:938967/1‑149 (MQ=255)
                                                                               tgtgTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGt                                                                       <  1:1556795/149‑1 (MQ=255)
                                                                               ggtgtCAGTGCCGGTAATACCGGGGCGCGGATGGGGCGGGCAAAATTCTTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTCGGGGCCAACGt                                                                       <  2:1212899/148‑1 (MQ=255)
                                                                               ggtgtCAGTGCCCGTAATCCCGGGGCGCTGAGGGGGCTGGCAAAAATATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGt                                                                       <  1:1528222/148‑1 (MQ=255)
                                                                                gtgtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                      >  1:540953/1‑149 (MQ=255)
                                                                                gtgtCAGTGCCGGTAATACCGGGGCGCGGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTc                                                                      <  1:600131/149‑1 (MQ=255)
                                                                                  gtCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGa                                                                    >  1:404798/1‑149 (MQ=255)
                                                                                   tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAt                                                                   <  2:553747/149‑1 (MQ=255)
                                                                                   tCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAt                                                                   >  1:975898/1‑149 (MQ=255)
                                                                                     aGTGCCTGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATAGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtg                                                                 >  1:902725/1‑149 (MQ=255)
                                                                                      gTGCCGGTAATCCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAGTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgt                                                                <  1:658767/149‑1 (MQ=255)
                                                                                       tGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATtgtg                                                               >  1:349956/1‑149 (MQ=255)
                                                                                        gCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGa                                                              <  2:1239764/149‑1 (MQ=255)
                                                                                          cGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACa                                                            <  1:973490/149‑1 (MQ=255)
                                                                                                 aCCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAAt                                                     >  1:792222/1‑149 (MQ=255)
                                                                                                   cGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTTCCACATCAGCAAAAGGGCAAAACGGTGGTGCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGc                                                   <  1:574806/149‑1 (MQ=255)
                                                                                                     gggCGCTGAAGAGGCTGGCATAATTATTAATAAAGCCACTGGAGGGGATAGAGAGTCCGCAGCTGGTGACGGTATTAACACATCAGCAAAAGGGAAAAACGTTGGTCCATGACTTAGGGTCCAACGTCGATTGTGACAGCACAAtcatg                                                 >  2:736614/1‑145 (MQ=255)
                                                                                                        cgcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTg                                              >  2:835761/1‑149 (MQ=255)
                                                                                                         gcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAACGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGc                                             >  1:1029567/1‑149 (MQ=255)
                                                                                                         gcTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGAGGTCGTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGc                                             >  1:624542/1‑149 (MQ=255)
                                                                                                             aTGGGGCTGGCAAAATTATAACTCATGCCCCTGGAGTGGATTGAGCTTCCGGCGCTGGTGACGGTATTACCACATCAGCTAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAAtt                                         <  2:1424933/149‑1 (MQ=255)
                                                                                                               ggggCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACTTCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTg                                       >  1:751138/1‑149 (MQ=255)
                                                                                                                gggCTGGCAAAATTATTACTCAAGGCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGTCGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGc                                      <  2:739729/149‑1 (MQ=255)
                                                                                                                     ggCAAAATTATTACTCAAGCCCCTGGAGGGGAGTGAGCGTCCGGCGCGGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTa                                 <  1:810064/149‑1 (MQ=255)
                                                                                                                      gCAAAATTATTACTCAAGCCGCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGGCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTAt                                <  1:1267593/149‑1 (MQ=255)
                                                                                                                              attaCTCAAGCCCCTGGAGCGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATGGTGTCAGCACAATGCTGGTGCTAGTTGCCATGATGGGCTCAg                        >  1:1083504/1‑149 (MQ=255)
                                                                                                                                taCTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCGGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGtt                      <  2:1107176/149‑1 (MQ=255)
                                                                                                                                  cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGCCTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCt                    >  2:1224847/1‑149 (MQ=255)
                                                                                                                                  cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCAGTATGGGCTCAGTTCt                    >  2:407631/1‑149 (MQ=255)
                                                                                                                                  cTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGAATGTGACACCACAATGCTGGTGCAATTTTCCATTATGGGCTCAGTTCt                    >  2:634462/1‑149 (MQ=255)
                                                                                                                                   tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCTCAATGCTGGTGCAAGTTGCCATTATGGGCTCAGTTCTg                   >  1:457344/1‑149 (MQ=255)
                                                                                                                                   tCAATCCCCTCCAGGGGCTTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTg                   >  2:1557410/1‑149 (MQ=255)
                                                                                                                                   tCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTACTg                   >  1:746441/1‑149 (MQ=255)
                                                                                                                                    aaaGCCCCTGGAGGGGATTGAGCGGCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTgg                  <  1:808297/148‑1 (MQ=255)
                                                                                                                                       gCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTg               >  2:263090/1‑149 (MQ=255)
                                                                                                                                         cccTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGaa             <  2:404798/149‑1 (MQ=255)
                                                                                                                                            gggAGGGTATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGGCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAagag          <  1:745357/148‑1 (MQ=255)
                                                                                                                                              gAGGGGATTGAGCGTCCGGCGCTGGTGCCGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGGTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAcgt        >  1:939607/1‑149 (MQ=255)
                                                                                                                                                 gggATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAggtggt     >  1:1319027/1‑149 (MQ=255)
                                                                                                                                                    aTTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGAGGTGGa  >  2:707206/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACATCAGCAAAAGGGCAAAACGGTGGTCCTTGACTTAGGGGCCAACGTCGATTGTGACAGCACAATGCTGGTGCAATTTGCCATTATGGGCTCAGTTCTGGCTGAAGAGGTGGTGGA  >  NC_000913/1147845‑1148141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: