Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,380 T→C 5.7% intergenic (+50/+32) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3800TC 5.7% 293.9 / 7.1 106intergenic (+50/+32)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (50/48);  new base C (4/2);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 107 total aligned reads displayed.

CTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGA  >  NC_000913/1169232‑1169525
                                                                                                                                                    |                                                                                                                                                 
cTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTACCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGCCAt                                                                                                                                                   >  1:428197/1‑149 (MQ=255)
     gcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTATTTTATAAATAAGCATAAACCGTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                                                              >  2:700501/1‑149 (MQ=255)
     gcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTTACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGACgt                                                                                                                                              >  1:872055/1‑149 (MQ=255)
       aaaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTACCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATTAGGATTAACCCTCATTTAGGCCTGCTACTGCTGATTTTTTCCCCGCGACATGGCgtgt                                                                                                                                            >  1:914518/1‑149 (MQ=255)
       aaaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCAGGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCGGCTTTTTTCCCCGCGACAGGCCgggt                                                                                                                                            >  2:859435/1‑149 (MQ=255)
         aaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcg                                                                                                                                          >  1:1237700/1‑149 (MQ=255)
           aGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcgcg                                                                                                                                        <  2:1101757/149‑1 (MQ=255)
           aGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTcgcg                                                                                                                                        <  1:1348859/149‑1 (MQ=255)
             cGGATGAGATGGGCGCAAAAGCTTTCCGGATTACGTCTGTAACCGGTCCGACTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                                                                      <  2:1142698/149‑1 (MQ=255)
                     aTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                              <  1:1226718/149‑1 (MQ=255)
                       gggCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTa                                                                                                                            >  1:1180238/1‑149 (MQ=255)
                       gggCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTa                                                                                                                            >  1:401033/1‑149 (MQ=255)
                            cAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCCTTAATGCCTGCTACTACTGATTTTCTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCgg                                                                                                                       <  2:331027/149‑1 (MQ=255)
                             aaaaTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCggg                                                                                                                      >  2:47790/1‑149 (MQ=255)
                                 tCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTTTAAATAAGCATTAACCCTCAGTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCGGTGTCGCCGGGATTTTTTTATCCggtcgtt                                                                                                                  >  2:1516805/1‑144 (MQ=255)
                                    ttCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACa                                                                                                               <  1:935263/149‑1 (MQ=255)
                                       cGTATTACTTCTGTACCCGGTCCGAATACCCTCCATGGAACAGCAGTAAGTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGATGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTGATACGGGCTGTACAGCg                                                                                                            >  2:1049493/1‑149 (MQ=255)
                                        tatttACTTTTGAAACCGTACCTAAAACCCTCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGtt                                                                                                          <  1:1031536/146‑1 (MQ=255)
                                          aTTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGttt                                                                                                         <  1:808889/149‑1 (MQ=255)
                                           ttACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtg                                                                                                        >  2:1440771/1‑149 (MQ=255)
                                           ttACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtg                                                                                                        >  2:586914/1‑149 (MQ=255)
                                            tACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgt                                                                                                       <  2:1091356/149‑1 (MQ=255)
                                             aCTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgtg                                                                                                      <  1:1449797/149‑1 (MQ=255)
                                                 cTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCt                                                                                                  >  1:314546/1‑149 (MQ=255)
                                                    tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTACCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATTCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                                                               >  2:1089652/1‑149 (MQ=255)
                                                    tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                                                               >  1:11277/1‑149 (MQ=255)
                                                    tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCAc                                                                                               <  2:1220083/149‑1 (MQ=255)
                                                       ccGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAGTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                                                                            >  2:494924/1‑149 (MQ=255)
                                                        cGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgt                                                                                           >  2:202051/1‑148 (MQ=255)
                                                        cGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                           <  1:26133/149‑1 (MQ=255)
                                                        cGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATAAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtga                                                                                           <  2:1126659/149‑1 (MQ=255)
                                                          gTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCGTTACAGCGTTTGTGGGCGCACTtgatg                                                                                         >  1:698548/1‑149 (MQ=255)
                                                           tCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatga                                                                                        <  2:1237700/149‑1 (MQ=255)
                                                            ccGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAc                                                                                       <  1:1557428/149‑1 (MQ=255)
                                                              gAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcgg                                                                                     <  1:47790/149‑1 (MQ=255)
                                                                aTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                                                                   <  1:575063/149‑1 (MQ=255)
                                                                   cccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGGGTCGCGGGGATTTTTTTCTCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGAc                                                                                <  2:464800/149‑1 (MQ=255)
                                                                    ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGGGTTTGTGGGCTCACTTGATGCCGGCGGACa                                                                               >  2:670443/1‑149 (MQ=255)
                                                                    ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCGCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATGTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACa                                                                               >  1:77893/1‑149 (MQ=255)
                                                                        cATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTACCCCGCGACATGCGGGGTCGCGGGGTTTTTTTTTGCCGGGCTTAACGGCGTTTGTGGGCGCCATTGATGCCGGCGGCCAGccc                                                                           >  1:470096/1‑148 (MQ=255)
                                                                          tGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCAcc                                                                         >  1:1272800/1‑149 (MQ=255)
                                                                           ggAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGGGTCGCGGGGATTTTTTTATCCGGGATTTACCGCGTTTGTGGGCTCGCTTGCTGACGGCGGACATCCACCg                                                                        >  1:845874/1‑149 (MQ=255)
                                                                            gAACAGCAGTAATTTATAACTAAGCATTCACCCTCATTAATGCCTGCTACTGCTGATTTATTCCCCGCGCCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCGTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCgg                                                                       <  2:905476/149‑1 (MQ=255)
                                                                              ccagcagTAATTTATAAATAAGCATTCACCCTCATTACTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCa                                                                     <  1:479382/148‑1 (MQ=255)
                                                                                agcagTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACCGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTATACAGCGTTTGGGGGCTCCCTTGATGACGGCGGACATCCCCCGACagc                                                                   >  2:1357787/1‑147 (MQ=255)
                                                                                  cagTAATTTCTAAATACGCATGTAGCCGCTTTAAACCCGGCTTCAGTTGATTGTTTCCGGGCGTAATTCATGTTCGAGGTGATTTTTTATTCAGGTCTTTACAGCGTTTGTGGGCTCTCTTGATGACGGCGGACTTCCACCGGCATccc                                                                 <  2:275509/149‑1 (MQ=255)
                                                                                   agTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGTATTTTTTTATCCGGGCTTTAAAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCg                                                                >  1:377999/1‑149 (MQ=255)
                                                                                   agTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCg                                                                >  1:503253/1‑149 (MQ=255)
                                                                                    gTAATTTATAAATAAGCATTAACCCTCATTAATGCATGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGCGATTTTTTAATCCGGGCTTTACAGCGGTTGTGGGCTCACATTATGCCTGAGGAAATCCACCGGCATcaggg                                                               >  2:189203/1‑145 (MQ=255)
                                                                                        tttATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg                                                           <  1:1078192/149‑1 (MQ=255)
                                                                                          tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                                                         >  2:528701/1‑149 (MQ=255)
                                                                                          tataAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGa                                                         <  2:335667/149‑1 (MQ=255)
                                                                                             aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGActt                                                      >  1:1225769/1‑147 (MQ=255)
                                                                                             aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcat                                                      <  1:859435/149‑1 (MQ=255)
                                                                                             aaataaGCATTAACCCTCAGTAATGCCTGCTACTGCTTATTTTTTCCCCGCGCCCTGCCGTGTCGCGGGGAGTGTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCCCATGAGGACGGCGGACAGCCACCGGCCTCCCGGAGCGGAcat                                                      >  2:1566333/1‑149 (MQ=255)
                                                                                              aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatc                                                     <  2:5326/149‑1 (MQ=255)
                                                                                               ataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                    >  2:497458/1‑149 (MQ=255)
                                                                                                  aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACATCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatc                                                 <  2:137285/149‑1 (MQ=255)
                                                                                                  aGCATTAACCCTCATTAATGCCTGCTACTGCTGATGTTTTCCCCGCGACATGCCGGGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatc                                                 <  2:1259463/149‑1 (MQ=255)
                                                                                                   gCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATca                                                <  2:572913/149‑1 (MQ=255)
                                                                                                    cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcac                                               >  1:695933/1‑149 (MQ=255)
                                                                                                      ttAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACAt                                             <  2:1515288/149‑1 (MQ=255)
                                                                                                         aCCCTCATTAATGCCTGCTACTGCTGATTTTATCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                          <  2:914518/149‑1 (MQ=255)
                                                                                                         aCCCTCATTAATGCCTGATACTGCTGATTTTTTCCCCTCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                          <  1:126774/149‑1 (MQ=255)
                                                                                                          cccTCAATACTGCCTGCTACTGCTGATTTTTTCCCCGCGACCTGCCGTGGCGCGGTGATTTTTTTATCCGGGCTTTACCGCGTTTGTGGGCTCACTTGATGACGGCGGACAGCCACCGGCATCCCGGAACGGACATCCATCACATGTTg                                         <  2:1329383/149‑1 (MQ=255)
                                                                                                           ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATTCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTga                                        >  2:682673/1‑148 (MQ=255)
                                                                                                           ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCTGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGc                                        >  2:515392/1‑149 (MQ=255)
                                                                                                           ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGCCATCCCCCGGCATCCCGGAACGGAGATACATGACATGTTGc                                        >  1:1062963/1‑149 (MQ=255)
                                                                                                            cTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGATTTACAGCGTTTGTGGGCTCCCTTGATGACGGCGGACATCCACCGGCATCCCGGAACGCACAGCCGTCCCATGTTGCa                                       >  2:280657/1‑149 (MQ=255)
                                                                                                            cTCAATTATGCCTGCTACTTCTGATTTTTTAACAGGGAGATTCCGTGTAGAGGGGATTTTTTTATCCGGGATTTAATTCGTTTGTTGGCTCAATAGATGACTGCGGACATCCATCGTCATCCCGGAACGTACATCCATCACATGTTGCa                                       <  2:411225/149‑1 (MQ=255)
                                                                                                               aTTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCa                                    >  2:501784/1‑149 (MQ=255)
                                                                                                               aTTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCa                                    >  1:459670/1‑149 (MQ=255)
                                                                                                                  aaTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACtt                                 >  2:913184/1‑149 (MQ=255)
                                                                                                                  aaTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACtt                                 <  2:55442/149‑1 (MQ=255)
                                                                                                                   aTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGCACGGACATCCATCACATGTTGCATCACTTc                                >  1:1338683/1‑149 (MQ=255)
                                                                                                                          cTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCa                         <  1:134166/149‑1 (MQ=255)
                                                                                                                           tACTGCTGATTTTTTCCCCGCGACATTCCGTGGCGATGGGATTTTTTTATCCTGGGTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGCCATACACCGGCATCCCGGACCGGACATCCCTCACATGGTGCCGCCCTTCAGCGTCAg                        >  1:1049330/1‑149 (MQ=255)
                                                                                                                             ctgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGtt                      >  1:1175110/1‑149 (MQ=255)
                                                                                                                              tgctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGttt                     <  2:1180238/149‑1 (MQ=255)
                                                                                                                                ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                   <  2:902272/149‑1 (MQ=255)
                                                                                                                                  gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACAGCCATCACATGTTGCATGACTTCAGCGTCTGTTtgtgg                 >  1:506321/1‑148 (MQ=255)
                                                                                                                                  gATTTTGTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCTGTTTTtgc                 <  2:178966/149‑1 (MQ=255)
                                                                                                                                   aTTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACGTCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                >  1:847042/1‑149 (MQ=255)
                                                                                                                                    ttttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgctg               <  1:700501/149‑1 (MQ=255)
                                                                                                                                    aaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgctg               >  2:590438/6‑149 (MQ=255)
                                                                                                                                    aaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGCCTTTACAGCGTTTGAGTGCTGACTTGATGAAGGCGGACATCCACCGGCATCCCGGAACGGACATCCAGCACATGTTGCATCCCTTCAGAGTCAGTTTGagctg               >  2:476904/6‑149 (MQ=255)
                                                                                                                                     tttttCCCCGCTACATGACTTGTCGCGTGTATTTTTTTATTCGGGCTTTAGTGCTTTTGTGGGATCACTTGATGACGGCTTACATCCACCGGCATCCCGGAACGGACATCGATCACATTTTGCATCACTTCAGCGTCATTTTGtgctgc              <  1:82784/149‑1 (MQ=255)
                                                                                                                                     tttttCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTGGTGGGCTCACTTGATGACGGCGGACATCCGCCGGCATCCCGGAACGGGCGTCCATCAGAGGTTGCATGCCGTCCGCGTGAGTTGGtgcggg              >  2:1534983/1‑146 (MQ=255)
                                                                                                                                       tttCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTAACCGGGCTTTAGAGCGTTTGTTGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCAt            <  1:754972/149‑1 (MQ=255)
                                                                                                                                          ccccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCCCTTCAGCGTCAGTTTGTGCTGCATCtt         >  1:877906/1‑149 (MQ=255)
                                                                                                                                           cccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCCTCACTTCAGCGTCAGTTTGTGCTGCATCttt        >  1:729871/1‑149 (MQ=255)
                                                                                                                                            ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa       >  2:473901/1‑149 (MQ=255)
                                                                                                                                            ccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTATACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTCGATCACTTCAGCGTCAGTTTGTGCTGCTTCTTTa       >  2:1092737/1‑149 (MQ=255)
                                                                                                                                            ccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGCGGACATCCACCGGCATCCCGGCACGGACATCCATCACATGTTGCCTCACTTCAGCGTGAGTTTGTGCTGCATCTTTa       >  2:123104/1‑149 (MQ=255)
                                                                                                                                              gcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa     >  1:801721/1‑149 (MQ=255)
                                                                                                                                              gcgACATGCCGGGTCGGGGGGATTTTTTTATCCGGGCTTTACAGCGTGTGTGGGCTCCCTTGATGACGGCGGACATCCACCGGCATCCCGTAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa     <  2:845874/149‑1 (MQ=255)
                                                                                                                                               cgACATGCCGTGTCGCGGGGATTTTTTTATCCGTGCTTTACAGCGTTTGTGGGCTGACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATAACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAAt    <  1:911454/149‑1 (MQ=255)
                                                                                                                                               cgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTTTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAAt    <  2:872055/149‑1 (MQ=255)
                                                                                                                                               cgACATGCCGTGCCGGGGGGATTTTTTTATCCGGGCTTTACAGCGGTTGGGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAAt    <  2:428197/149‑1 (MQ=255)
                                                                                                                                                 aCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTTAATGa  >  2:451638/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGA  >  NC_000913/1169232‑1169525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: