| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 126,126 | G→A | 7.4% | K144K (AAG→AAA) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,126 | 0 | G | A | 7.4% | 226.0 / 4.3 | 93 | K144K (AAG→AAA) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (30/54); new base A (4/3); total (35/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAG > NC_000913/125978‑126274 | cTCCTGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTTGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAg < 1:11220/149‑1 (MQ=255) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg > 1:65231/1‑149 (MQ=255) cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAGCACCGTAGAAGGCGACAAGGCt > 2:1294481/1‑149 (MQ=255) cGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:1246244/1‑149 (MQ=255) agcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa > 1:1126016/1‑149 (MQ=255) atcaCCAGCGGCTGCTGCTGCAAAAGACGTTAACGTTCCGGATATCGGCATCGACGAAGTTGAAGTGATCGATATCCTTGTGAAAGTTGGCTATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg < 2:307314/147‑1 (MQ=21) agcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg < 1:583996/149‑1 (MQ=255) gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGATAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa > 1:1363996/1‑149 (MQ=255) gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGa < 2:119526/149‑1 (MQ=255) gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGGGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGACGCTGATCACCGTAGACGGCGAAAAGGCTTcgagtga > 2:853188/1‑143 (MQ=25) cAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt > 1:386524/1‑149 (MQ=255) aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTc < 1:14707/149‑1 (MQ=255) gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGAGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc > 1:338936/1‑149 (MQ=255) ggCTGCGGCGGCAAAAGAGGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGGTAAAGTTGAAGCTGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg < 1:872480/149‑1 (MQ=255) ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg < 2:165274/149‑1 (MQ=255) gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:324090/149‑1 (MQ=255) gcAAAATACCTTAACGTACCGCAAATCGGCAGCGACGAATTTGATTTTTATTAAATCCTGGTGAAATTTTTCGATAATGTTGAATCTTAACATTCGCTGATCACGGTAGATGGCGACAATGCTTCTATGGAAGTTCCGGCTCCGTTTGc < 2:1518707/149‑1 (MQ=255) aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg < 1:643056/149‑1 (MQ=255) aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGAAGAAGGCGACATTGCTTCTATGGAAGTTCAGGCTCCGGTTGCTg > 2:1445402/1‑149 (MQ=18) aaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGTCCGAAATCCTGGTGTAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTTGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg < 2:392072/149‑1 (MQ=255) aGACGCTAACGTTCCGGATCTCTGAAGCGACGAAGTTGAAGTGACCGAACTCCGGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCa < 2:1024390/149‑1 (MQ=18) aCGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAcc < 1:498716/149‑1 (MQ=255) aCGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCTTGaaa < 1:702113/149‑1 (MQ=255) ttCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 1:1247458/149‑1 (MQ=255) tCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCATTTGCTGGCACCGTGAAAgaga < 1:1428941/149‑1 (MQ=255) atCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCAGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTTGCACCGTGAAAGAGATCAAAGt > 1:849241/1‑149 (MQ=255) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGCACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGaa > 1:892877/1‑149 (MQ=255) gCAGCGACGAAGCAGACGTGACCTAAATCCTGGTGTAATTTGGCGATAAAGTTGATGCTGAACAGTCGCTTATCACCGTAGAAGCCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGTAc < 2:597903/149‑1 (MQ=255) cttcgtaGTTGAATTGACCGTTATCGTGGTGTAAGTTGGCTATAATGTTTAAGCTGAACTGTGGCTTTTCAGCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg < 1:145781/143‑1 (MQ=255) acgaAGTTGAAGTGACCGAAATCCTGGGGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt < 1:616373/149‑1 (MQ=255) ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACaaa > 1:142090/1‑149 (MQ=255) gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgt < 1:303602/149‑1 (MQ=255) aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACTTGGGTGACAAAGTGTCTAc < 2:65231/149‑1 (MQ=255) aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAc < 1:1382288/149‑1 (MQ=255) cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGcc < 1:1114666/93‑1 (MQ=255) cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGcc > 2:1114666/1‑93 (MQ=255) cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCg < 1:820349/149‑1 (MQ=255) aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGc < 1:293775/101‑1 (MQ=14) aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGc > 2:17292/1‑149 (MQ=255) aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGc < 2:1239465/149‑1 (MQ=255) aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCAGGc > 2:293775/1‑101 (MQ=255) aaaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGGTTGCGGGCAGCGTGAAAGAGATCAAAGTGCACGTGGGTGACAAAGTGTCTACCGGc < 2:422541/149‑1 (MQ=21) cTGGGGAAAGTTGGCGATAAAGTTGCAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCATAGTGAACGTTGGTGACAAAGTGTCTACCGGCTCGCt < 2:165943/149‑1 (MQ=255) tcaaaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg < 2:427984/118‑1 (MQ=255) tcaaaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTgg > 1:427984/3‑120 (MQ=17) acgTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGCCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATg < 1:937386/147‑1 (MQ=255) aaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa < 2:226374/98‑1 (MQ=255) aaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCATCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGaaa > 1:226374/1‑98 (MQ=14) ggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGaa < 2:404833/63‑1 (MQ=255) ggCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGaa > 1:404833/1‑63 (MQ=255) ggCGATAAAGTTGAAGCTGAACAGTCGCGGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCtt < 2:849241/149‑1 (MQ=255) gCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACAGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTc > 1:5746/1‑149 (MQ=255) gCGATAAAGGTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTc < 1:85374/149‑1 (MQ=255) gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGa > 2:1322550/1‑149 (MQ=255) gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGa > 1:1444524/1‑149 (MQ=255) aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa < 2:1246244/149‑1 (MQ=255) aTAAAGTTGAAGCTGAACAGTCGCTGATCACCCTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCGGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGaa < 2:792992/149‑1 (MQ=255) aaaGTTGAAGCTGAACAGTCGCTGATGACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAACGAGCTCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCGTCGACGt > 1:1017830/1‑149 (MQ=255) aGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCCCCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTGCCGGCGCGCGGATTATGGGCTTGGAAGTcg > 1:118991/1‑149 (MQ=25) ttGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg < 1:1087990/149‑1 (MQ=255) ttGAAGCTGAACAGTCGCTGAGCACCGTAGAAGGCGACAAGGCTGCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGGGAAAGAGCTCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTcgcg < 1:167122/149‑1 (MQ=255) ttGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 1:1410897/1‑89 (MQ=255) ttGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc < 2:1410897/89‑1 (MQ=255) tGAAGCTGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCgg < 1:719983/149‑1 (MQ=255) aaGCTGAACAGTCGCTGATCACCTTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGt < 2:338936/149‑1 (MQ=255) aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTg > 2:744828/1‑149 (MQ=255) aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTACTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGATATCAAAGTGAACGTTGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTg < 1:588144/149‑1 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGttt > 1:295805/1‑63 (MQ=255) cTGAACAGGCGCTGAGCACCCTAGAAGGCGAGAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGACCGTGGGTGACAATGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGaa < 2:1363996/149‑1 (MQ=255) tcaCAGTCGCGGCTCACCGTAGATGGCGACAAGGCTTCTATGGATGTTCCGGCTACGTTTGCTGGCACCGGGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGTTTATGTTCTTCGAAGTCGCGGGTGAAGc < 1:853188/147‑1 (MQ=255) gAACAGTCGCTTATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTATGCTGGCACCGTGAAAGAGCTCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGc < 2:1477773/149‑1 (MQ=255) gAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGc > 2:17932/1‑149 (MQ=255) aCAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGGGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAg < 2:1443144/149‑1 (MQ=255) cAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAgg > 2:1240072/1‑149 (MQ=255) cAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAGCAAAGTGAACGTGGGTGACAAAGTGGCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAgg < 2:625423/149‑1 (MQ=255) gTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGACCGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGGGAAGCAGgcg < 1:1319133/149‑1 (MQ=255) tCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgcgc > 2:865528/1‑149 (MQ=255) tCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGgcgc > 2:110086/1‑149 (MQ=255) gCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCgg > 1:173220/1‑149 (MQ=255) cTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTGCCGGCGCCGTTTGCTGGCACCGGGAAAGAGAGCAAAGTGAACGTGGGGGACAAAGTGTCTACCGGCGCGCTGATTATGGTCTTCGAAGTCGCGGGGGAAGCAGGCGCGGc < 1:699569/149‑1 (MQ=25) gATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCac > 1:553316/1‑148 (MQ=255) gATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAg < 1:822628/149‑1 (MQ=255) aCCGTAGAAGGCGACAAGCCTTCTATGGAAGTTCCGGCTCCGTTTGCTGCCACCGTGAAAGAGATCAACGTGAACGGGGCTGACAAAGTGTCTACCGGCGCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTcc < 2:197108/149‑1 (MQ=255) ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGTATCCGTTTGCTGGCACCGTGAAAGAGATCAAATTGACCGTGTGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCg < 1:1503876/149‑1 (MQ=255) ccGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAACTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCg > 1:533181/1‑149 (MQ=255) gTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTTGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGc > 2:950481/1‑149 (MQ=255) ctAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAACGGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGc < 1:1563102/148‑1 (MQ=255) ggatggCGACAAGGCTTCAAAGGACGATCCCCCTCCGGATGCGGGCACGGTCAACCACATCACAGGGCCCGGGGGTGACACACTGGCGACCGGCGCGCTGATTCTGGTCTTCGATGTCGCGGGAGACGCAGGCGCGGCAGCTCCGGCCg < 2:1168604/145‑1 (MQ=255) aaGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGCAGCAGGCGCGGCAGCTCCGGCCGCt > 2:1521262/1‑149 (MQ=255) cGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAAc < 2:619634/149‑1 (MQ=255) cGACAAGGCTTCTATGGAAGCTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAAc < 2:386524/149‑1 (MQ=255) gACAAGGCTTCTATTGAAGTTCCGCCGCCCTTTGCTGGCACCGTCAAAGACATCAAAGTGAACGGGGGTGACAACGTGCCTACCGGCTCTCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACa < 1:1195264/149‑1 (MQ=255) gACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACAGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACa > 2:777215/1‑149 (MQ=255) aCAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAg < 2:1126016/149‑1 (MQ=255) aaGGCATCTATGGAAGTTCCGGCTCCGTTTGATGGAACAGTGAAAGACCTCAAAGTCAACGTGGGTGACAAAGTTTCTAAAGTCTCGATGATTATGTTCTTATAAGTCGCGGTTGAAGCATTCTCGGCAGCTCCGGCCGCTAAACAGGa < 2:1074230/149‑1 (MQ=255) tggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGTGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGaa < 1:1445402/148‑1 (MQ=255) ggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAag > 1:1302849/1‑149 (MQ=255) ggCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAag < 1:724101/149‑1 (MQ=255) | CTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGAAGCAGGCGCGGCAGCTCCGGCCGCTAAACAGGAAG > NC_000913/125978‑126274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |