Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,344,033 A→G 7.5% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,344,0330AG 7.5% 247.5 / 10.0 93L105P (CTG→CCG) yciTglobal regulator of transcription; DeoR family
Reads supporting (aligned to +/- strand):  ref base A (45/40);  new base G (5/2);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

GCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCG  >  NC_000913/1343885‑1344180
                                                                                                                                                    |                                                                                                                                                   
gCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCCCCGAGCAAAATAACTTCACAACGCGCGTCTTTCAGCAAAAGCGCGATGAAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCTCCCa                                                                                                                                                     >  1:917317/1‑149 (MQ=255)
gCACTGGCGGGTCAAAGGGCCAACCATACTTGCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAATTGCGCGATGTAGCTGCTGACCGTGATGAAAGTGACATTTTTCTTCTGCTCGCCCa                                                                                                                                                     <  2:1220844/149‑1 (MQ=255)
       cGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCg                                                                                                                                              <  2:1004587/149‑1 (MQ=255)
         tgtCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCggg                                                                                                                                            <  1:1382082/149‑1 (MQ=255)
          gtCTAAGTGCCAACCATAGTATCGCTTTTTTTCTGTTACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGc                                                                                                                                           <  2:917317/149‑1 (MQ=255)
          gtCAAAGGGCCAACCATAGTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGAGGTAGCTGCTGCCCGTGATGATAGTGCCATTTTTCTTCTGCTCGCCCAGAGTCCGGGc                                                                                                                                           >  2:796526/1‑149 (MQ=255)
            cAAATGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGAGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                                                                         <  1:402915/149‑1 (MQ=255)
            cAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTGACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGa                                                                                                                                         <  1:973362/149‑1 (MQ=255)
            cAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGCCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGATTCCGGGCGa                                                                                                                                         >  1:1021574/1‑149 (MQ=255)
            cAAAGGGCCAACCATACATTCGCTTTTTATCTGATACACGCCACCGAGCAAAATAACTTCACAACGCGCGTCTTTCAGCAAAAGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGa                                                                                                                                         <  2:696155/149‑1 (MQ=255)
             aaaGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGAAATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAg                                                                                                                                        >  2:1476224/1‑149 (MQ=255)
                gggCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCGGACTCTGGGCGAGCAg                                                                                                                                     <  2:1270320/149‑1 (MQ=255)
                 ggCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                                                                    >  2:976301/1‑149 (MQ=255)
                 ggCAAACCATACTTTCGCTTTTTTTCTGATACACGCCACGGAGCACAATAACTTCACAAGGCGCGTCTTTCAGAAAATGCGCTATGTAGCTGCTGACCTTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAgg                                                                                                                                    <  2:291960/149‑1 (MQ=255)
                  gCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCAAGAGTCCGGGCGAGTAggg                                                                                                                                   >  1:856541/1‑149 (MQ=255)
                   ccAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCCCAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGATCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                                                                  >  1:165994/1‑149 (MQ=255)
                   ccAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                                                                  >  1:155913/1‑149 (MQ=255)
                   ccAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGc                                                                                                                                  >  1:438693/1‑149 (MQ=255)
                    cAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGCGCa                                                                                                                                 >  2:239948/1‑149 (MQ=255)
                         aTACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGAGATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtggt                                                                                                                            >  1:721137/1‑147 (MQ=255)
                         aTACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCGTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgat                                                                                                                            >  2:1426850/1‑147 (MQ=255)
                         aTACTTTCGATTTTTTTCTGATACACGCCCCCGAGCAAAATAACTTCCCAAGGCGCGTCTTTCAGCAAATGCGCGAGGTAGCTGCTGACCGTGATGCTAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgct                                                                                                                            <  2:405842/149‑1 (MQ=255)
                            atttCGCTTCTTTTCTGATACACTCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGAATTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgct                                                                                                                         <  2:134640/148‑1 (MQ=255)
                             tttCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATtgctgctg                                                                                                                        >  2:7330/1‑149 (MQ=255)
                                 gCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAt                                                                                                                    >  1:191410/1‑149 (MQ=255)
                                  cTTTTTTTCTGATACACGCCACCGAGCAAATTACCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtt                                                                                                                   >  2:1295661/1‑149 (MQ=255)
                                   tttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt                                                                                                                  >  2:1265809/1‑149 (MQ=255)
                                   tttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCCTGATGATAGTGACATGTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAttt                                                                                                                  >  2:722136/1‑149 (MQ=255)
                                    ttttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCAtttt                                                                                                                 >  2:368336/1‑149 (MQ=255)
                                     tttttCTGATACACGCCACCGAGCAAAATACCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATTATCGTGAGATTTTTCTTCTGCTCGCCGAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                                                                <  2:89238/149‑1 (MQ=255)
                                     tttttCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTc                                                                                                                <  2:405381/149‑1 (MQ=255)
                                          gtGATACTCGCCACGGAGCAAATTAACTTGACATGGCGCGTCTTTCAGCAAATGCGCGATGTTGCTGCTGAGCGTGATGATTGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaa                                                                                                           <  2:321061/148‑1 (MQ=255)
                                           tGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                                                                          >  2:1384763/1‑149 (MQ=255)
                                           tGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCAGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATaaa                                                                                                          >  1:58597/1‑149 (MQ=255)
                                            gATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAg                                                                                                         <  1:765351/149‑1 (MQ=255)
                                               acacGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATg                                                                                                      <  1:471583/149‑1 (MQ=255)
                                                 acGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGt                                                                                                    >  2:1531758/1‑149 (MQ=255)
                                                  cGCCACCGAGCAAAATAACTGCAGAAGGCGCGTCTTTCAGCAAATGCGCGAGGTAGCTGCGGCCCGGGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATATAGATGGtt                                                                                                   <  2:1382709/149‑1 (MQ=255)
                                                   gCCACCGAGCAAAATAACTTCTCAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGttt                                                                                                  <  2:1482014/149‑1 (MQ=255)
                                                    ccACCGATCATAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                                                                 <  2:165994/149‑1 (MQ=255)
                                                    ccACCGAGCAAAATAAGTTCACACTGCGCGTCTTTCAGCAAATGCGCGATGTAGCTTCGGACCGTGAGGATAGTGACATTTTTCTACTGCTCGCCCAGAGTCCCTGCGAGTAGGGCATTGCTGCTTCCATTTTCGATAAAGATGGTTTc                                                                                                 <  2:1037618/149‑1 (MQ=255)
                                                    ccACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTc                                                                                                 <  1:1292967/149‑1 (MQ=255)
                                                       ccGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGcc                                                                                              >  1:359556/1‑149 (MQ=255)
                                                         gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCGAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCg                                                                                            <  1:580693/149‑1 (MQ=255)
                                                         gAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCg                                                                                            <  2:1471562/149‑1 (MQ=255)
                                                          aGCAAAATACCTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCgg                                                                                           >  1:107128/1‑149 (MQ=255)
                                                           gCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCTCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGt                                                                                          <  2:1308207/149‑1 (MQ=255)
                                                            ctaaaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGtt                                                                                         <  2:568125/147‑1 (MQ=255)
                                                               aaTAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTCCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGCTTGaa                                                                                      >  1:697077/1‑149 (MQ=255)
                                                                 tAATTTCACATGGCGCGTTTTTCTGCAAATGCGCGATGTAGCTGCTGCGGGTGATGATAGTGACTTTTTTCTTCTTATCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCAATTTTCGATAAAGATGGTTTCGCCCGGTTGAAcc                                                                                    <  1:1528457/149‑1 (MQ=255)
                                                                        acaAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGAcg                                                                             >  2:1116420/1‑149 (MQ=255)
                                                                            ggCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGc                                                                         <  2:15193/149‑1 (MQ=255)
                                                                             gcgcGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCa                                                                        >  1:396050/1‑149 (MQ=255)
                                                                                   cTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg                                                                  >  1:961202/1‑149 (MQ=255)
                                                                                   cTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGACATTTTCGATAAACATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCg                                                                  >  1:357675/1‑149 (MQ=255)
                                                                                     ttCAGCAAATGCGCGATGTAGCTGCTGAGCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                                <  2:1523923/149‑1 (MQ=255)
                                                                                     ttCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGGGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGc                                                                <  1:251598/149‑1 (MQ=255)
                                                                                       cAGCTACTGCGCCATGTAGCTGCTGACCGTGATGATCGGGACCATTTTCTGCTGCGCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCTTTTTCGATAAAGATGGTTTCGCCCGGTTGAACGAGCGACGCGGCAAACTCGGCGa                                                              <  2:864395/149‑1 (MQ=255)
                                                                                        aGCAAATGCGCTATGTAGCGTCTGAGCGTGATGATAGTGACATTTTTCTTCTGCTCTCCCAGATTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTGCGCCCGGTTTAACCAGCGACGCGGCAAACTCGGCGAg                                                             <  2:1158459/149‑1 (MQ=255)
                                                                                                 gcgATGTAGCTCCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCGACCATTTTCGATAAAGATGGTTACGACCGCTTGCACCAGCGACGCGGCAAACTCGGCGAGTTCACGCtt                                                    >  2:1208673/1‑149 (MQ=255)
                                                                                                 gcgATGAAGCTGCTGACCGTGATGATAGAGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGATTCGCCCGGTTGAACCAGCGACGCGGCAAACTCTGCGAGTTCACTCtt                                                    >  2:1549739/1‑149 (MQ=255)
                                                                                                     tGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGc                                                <  2:191410/149‑1 (MQ=255)
                                                                                                       tAGCTGCTGCCCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGCGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAAATCGGCGAGTTCACGCTTCAGcct                                              >  2:779884/1‑147 (MQ=255)
                                                                                                       tAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGt                                              >  1:250101/1‑149 (MQ=255)
                                                                                                        aGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGta                                             >  2:1568066/1‑149 (MQ=255)
                                                                                                           tgctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAg                                          <  1:368336/149‑1 (MQ=255)
                                                                                                             ctgACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGGTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGAGAGTTCACGCTTCAGCGGATAGtt                                        >  2:107925/1‑149 (MQ=255)
                                                                                                                    tgatgaTAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatc                                 <  2:961202/149‑1 (MQ=255)
                                                                                                                     gatgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcatca                                <  1:1384763/149‑1 (MQ=255)
                                                                                                                      atgatAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGATTCGCCCGGTTGAACCAGCGACACGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCtcataaa                               >  2:1042045/1‑146 (MQ=255)
                                                                                                                        caaaGTGACATTATTCTTCTGCTCCCCCCCAGTCCGGGCGAGTAGGGCACTGCTGCAGCCATTATCGATAAAGATGGATTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATAc                             <  1:796526/146‑1 (MQ=255)
                                                                                                                          tAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACgg                           <  1:7330/149‑1 (MQ=255)
                                                                                                                           aGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACggg                          >  1:1116330/1‑149 (MQ=255)
                                                                                                                           aGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACggg                          >  1:1111591/1‑149 (MQ=255)
                                                                                                                            gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACg                            >  2:872129/1‑146 (MQ=255)
                                                                                                                            gTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCGGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACg                            <  1:872129/146‑1 (MQ=255)
                                                                                                                             tGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGtt                        >  2:14978/1‑149 (MQ=255)
                                                                                                                                 aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCgc                    >  1:653853/1‑148 (MQ=255)
                                                                                                                                 aTTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGa                    <  1:590621/149‑1 (MQ=255)
                                                                                                                                      tgccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATGTTCGATAAAGATGGTTTCGCCCGGTTGAACCGGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtca               >  1:1204157/8‑149 (MQ=255)
                                                                                                                                      tcttctGCTCGCCCAGAGTCCGCGCGAGTAGGGCATTGCTGCGGCCATTTTCGATAAAGATGGGTTCGCCCGGTTGAACCAGCGACGCGGCATCCTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGCGTTTCGACGtca               <  1:74672/149‑1 (MQ=255)
                                                                                                                                       cttctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGATCCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcat              <  2:58597/149‑1 (MQ=255)
                                                                                                                                        ccctacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACAAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGtcatc             >  1:1229690/6‑149 (MQ=255)
                                                                                                                                          ctGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCAc           >  1:283084/1‑149 (MQ=255)
                                                                                                                                           tacTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACt          >  2:892510/3‑149 (MQ=255)
                                                                                                                                            gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTa         >  1:1544906/1‑149 (MQ=255)
                                                                                                                                            gCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTa         <  1:976301/149‑1 (MQ=255)
                                                                                                                                            gCTCGCCAAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTa         <  2:856541/149‑1 (MQ=255)
                                                                                                                                             cTCGCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTAt        >  1:468077/1‑149 (MQ=255)
                                                                                                                                              tCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATCGTTTCGACCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTTTTGTGGCTCATCAGACGGGTTTCGACGTCATCACTATc       >  1:940476/1‑149 (MQ=255)
                                                                                                                                              tCGCCAAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCTATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCAGACGTGTATCGACGTCATAACGATc       >  1:1510900/1‑149 (MQ=255)
                                                                                                                                                gCCCGGACTCTGGGCGAGTAGGGCATTGCTGCTGCCATTGTCGATAAAGATGGTTTCGCCCGGTTGAACCATCGACGCGGCAAACTCGGCGAGTTCACGCTTAAGCGTATAGTTGATCATCATACGGGTTTCGACGTAATCACTATCaa     >  1:1462023/1‑149 (MQ=255)
                                                                                                                                                 cccAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTAACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAg    >  1:913560/1‑149 (MQ=255)
                                                                                                                                                   cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTTTAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCg  <  1:1265809/149‑1 (MQ=255)
                                                                                                                                                   cAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCg  >  1:1282444/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCACTGGCGTGTCAAAGGGCCAACCATACTTTCGCTTTTTTTCTGATACACGCCACCGAGCAAAATAACTTCACAAGGCGCGTCTTTCAGCAAATGCGCGATGTAGCTGCTGACCGTGATGATAGTGACATTTTTCTTCTGCTCGCCCAGAGTCCGGGCGAGTAGGGCATTGCTGCTGCCATTTTCGATAAAGATGGTTTCGCCCGGTTGAACCAGCGACGCGGCAAACTCGGCGAGTTCACGCTTCAGCGTATAGTTGCTCATCATACGGGTTTCGACGTCATCACTATCAAGCG  >  NC_000913/1343885‑1344180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑