Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,333	T→G	7.0%	E199A (GAG→GCG)	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,333	0	T	G	7.0%	199.9 / 3.4	83	E199A (GAG→GCG)	ydjH	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (37/40);  new base G (3/3);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

GACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACC  >  NC_000913/1856188‑1856481                                                                                                                                                  |                                                                                                                                                     gACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATATCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                                                                   <  2:201638/149‑1 (MQ=255) gACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTGTTACGCCGCACGCAATAAAGCAGTCAGCAATATCATCCAGTGTCTCGTTCCCGGTGAGTTATTTTGCCTAgg                                                                                                                                                   >  2:1021945/1‑149 (MQ=255)         gCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGGCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTa                                                                                                                                           <  1:448954/149‑1 (MQ=255)          cACCTTCATCGTCATGTCACCACCCTTGATAAACCAGCCGTCATTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAg                                                                                                                                          <  1:1564659/149‑1 (MQ=255)           acattCATCGTCATGTCACCACTCTTGATAAATCAGCCGTCTTTACACGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                                                                         <  2:880258/146‑1 (MQ=255)             cTTCATCGTCATGTCACCACGCTTTATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTAAGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGGCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTactca                                                                                                                                       <  1:1199096/149‑5 (MQ=255)             cTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGGAAGAAAGCAGTCGGCAAGTTCGTCGAGTGTCTCGTTCCCGGTGAGTAATTTTGCGTCGGGAAAATTAGGaa                                                                                                                                       >  2:650646/1‑149 (MQ=255)             cTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGaa                                                                                                                                       >  1:277099/1‑149 (MQ=255)             cTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCCTTTTAATCACCACCGTTTTTACGCCGCACGAAAGAAATGAGTCAGCAATTTCATCCAGTGTCTCTTTCCAGATCAGTAATTTTTCATCAGCAAAATTAGTaa                                                                                                                                       >  2:327556/1‑149 (MQ=255)                cATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACa                                                                                                                                    <  1:1259539/149‑1 (MQ=255)                  tCGTCATGTCTCCACGCTTGTTATAGCTGCCGTCTTTACCCGTTTTAATCAGCACCGTTTTAACGCCGAACGCAATAAAGTTGGCAGCTATTTCATCCAGTGTCTCTGTCCCTGTGAGTAATTTTGCCTCGGCAAATTTAGGAAACTGa                                                                                                                                  <  2:937318/149‑1 (MQ=255)                    gTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                                                                >  2:1253535/1‑149 (MQ=255)                    gTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATa                                                                                                                                >  2:130522/1‑149 (MQ=255)                     tCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCAGGTGAGTAATTTTGCCTCGGCAAAATTATGAAACAGATaa                                                                                                                               >  1:219824/1‑149 (MQ=255)                         ttCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                                                                           <  2:254271/148‑1 (MQ=255)                         gTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                                                                           <  1:137623/149‑1 (MQ=255)                         gTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGa                                                                                                                           <  1:618702/149‑1 (MQ=255)                          tCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGAc                                                                                                                          <  1:599961/149‑1 (MQ=255)                              cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATaa                                                                                                                      >  1:1294897/1‑149 (MQ=255)                                 gCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTc                                                                                                                   >  1:14547/1‑149 (MQ=255)                                   ttGATAAAGCAGCCGTCTTTTCCTGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAATGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGTGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCaa                                                                                                                 <  1:192985/149‑1 (MQ=255)                                   ttGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCAACGTTTTTACGCCGCACGCAATAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCaa                                                                                                                 >  2:848841/1‑149 (MQ=255)                                        aaaGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCtt                                                                                                            >  1:516654/1‑149 (MQ=255)                                           gcagcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCCCGCAAGAAAGCAGTCAGCAATTTCCTCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGCCATAGCTCAATGCTTCGc                                                                                                         >  1:500318/1‑149 (MQ=255)                                               ccGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGACACAGATAATCGACATAACTCACTGCTTCGCAAat                                                                                                     >  2:1124794/1‑149 (MQ=255)                                               ccGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAat                                                                                                     >  1:43292/1‑149 (MQ=255)                                                  gcTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGGGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatc                                                                                                  <  2:94231/148‑1 (MQ=255)                                                     ttACCCGTTTTAATCACCACCGTTTTTACGCCGAACGCGAGAAATCAGTCAGCAATTTCATCCAGTTTCTCTTTCCAGGTGAGTAATTTTTCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTAAATGCTTCGCAAATatcatc                                                                                               <  2:411493/149‑1 (MQ=255)                                                        cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCATCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAg                                                                                            >  2:685323/1‑149 (MQ=255)                                                         ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGc                                                                                           <  2:901870/149‑1 (MQ=255)                                                           gTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGt                                                                                         <  2:14547/149‑1 (MQ=255)                                                           gTTTTAATCACCACCGTTTTTACCCCTCACGCAAGAAAGCAGACACCAATTTCATCGAGTGTCTCTTTCCCGGAGAGTAATTTTGCCTCGTCAAAAATAGGAAACACATAATCGACATAACTAAATGCTACGCAAATATAATCCAGCGt                                                                                         >  2:402602/1‑149 (MQ=255)                                                                 aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTGTCTTTCCCGGTTAGTAATTTTTCCTCGGCAAAATTAGTAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcatt                                                                                   <  2:510833/149‑1 (MQ=255)                                                                      caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAcc                                                                              >  1:934458/1‑149 (MQ=255)                                                                      caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAcc                                                                              >  1:705628/1‑149 (MQ=255)                                                                         cGTTTTTACGCCGCTCGCAAGATAGCAGTCAGAAATTTGATCCAGTGTCTCTTTCCGGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                                                                           <  1:48962/149‑1 (MQ=255)                                                                           tttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGt                                                                         >  1:1256590/1‑149 (MQ=255)                                                                            ttttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGtt                                                                        >  1:820634/1‑149 (MQ=255)                                                                             tttACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                                                                       <  2:505540/149‑1 (MQ=255)                                                                               tACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGa                                                                     <  2:751965/149‑1 (MQ=255)                                                                                aCGCCGCACGCAAGAAAGGAGTCAGCAATTTCATCCAGGGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGATACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                                                                    <  2:859291/149‑1 (MQ=255)                                                                                 cgccgcACGCAAGAAAGCTGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATc                                                                   <  2:748339/149‑1 (MQ=255)                                                                                  gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTAATCCGCGGTTTGATCa                                                                  >  2:525153/1‑149 (MQ=255)                                                                                    cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                                                                >  1:139243/1‑149 (MQ=255)                                                                                      cacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTTAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATc                                                              <  1:1106523/149‑1 (MQ=255)                                                                                         gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGc                                                           >  2:1415191/1‑149 (MQ=255)                                                                                          caAGAAAGCAGTCAGAAATTTCATCCAGAGTCTCTTTCCCGGTGAGTAATTTTGCATCGGCAAAATTAGGAAACAGATAAACTACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCCCGCTTTGATCAAATCGGca                                                          >  1:1125429/1‑149 (MQ=255)                                                                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTTGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                                                                       <  1:688844/133‑1 (MQ=255)                                                                                             gAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTAGCAAATATCATCCAGCGTTTCATTCAACCGCGGttt                                                                       >  2:688844/1‑133 (MQ=255)                                                                                              aaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                                                                       >  1:445298/1‑116 (MQ=255)                                                                                              aaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                                                                       <  2:445298/116‑1 (MQ=255)                                                                                               aaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGaa                                                     >  2:1294925/1‑148 (MQ=255)                                                                                               aaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt                                                     <  2:577815/149‑1 (MQ=255)                                                                                                aGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATa                                                    >  2:878785/1‑149 (MQ=255)                                                                                                    gTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAAGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGATCTTTGATCATATCGGCACAGATAatca                                                >  2:150849/1‑149 (MQ=255)                                                                                                       aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCTAAATTAGGAAACAGATAATCGACAAAACTAAAAGAAACGAAAATATCATCCAACGTTTCATTCAACCGCGCTATAATCAAATCGGCACATAAAatcatca                                             >  1:1031238/1‑148 (MQ=255)                                                                                                       aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTAGCCTCGGCAAAATTAGGATCCAGATAATCGACATACCTCAATGCTTCGCTAATATCATACAGCTTGTCTTTCAACCGCGGTTTGATCATATCGGCACAGATAATCctct                                             >  1:1085569/1‑149 (MQ=255)                                                                                                          aaTTTCATCCAGTGTCTCTTTCCCGGTTAGTAATTTTGCCTCGTCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGcc                                          <  1:939463/149‑1 (MQ=255)                                                                                                           aTTTCATGCAGTGTCTGTTTCCAGGTGATTATTTTTGCCTCTGCAATATTAGGTAACAGATAATCGACATATCTGAATTCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCTCAGATAATCATCTGCCg                                         <  1:327556/149‑1 (MQ=255)                                                                                                                   ccgggtCTCTATCCCGGTGACTATATTTGCCGCGGCAAAATTAGGAAACAGATAATCGACATAACTCCATGCTTCGCAAATATCATCCACCGTTTCATTCAACCCCGGTTAGATCATATCGCCACAGATACTCAGCTGCCGGGCTTTAg                                 <  2:391964/145‑1 (MQ=255)                                                                                                                    aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGc                                <  1:872577/149‑1 (MQ=255)                                                                                                                      tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCtt                              <  1:130522/149‑1 (MQ=255)                                                                                                                      tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCtt                              <  1:1253535/149‑1 (MQ=255)                                                                                                                        gctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGGTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGc                            <  1:755983/148‑1 (MQ=255)                                                                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGGTTCATTCAACCGCGGTTTGAGCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGt                          <  1:848841/149‑1 (MQ=255)                                                                                                                          tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCGTCGCAAATATCATCCAGCGATGCCTTCAACCGCGGTTGGATCATATCGGCCCAGATAATCAGCTGCCGGGCTTAAGCTTGCGt                          >  2:933924/1‑149 (MQ=255)                                                                                                                            tttCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTaa                        <  1:1500654/149‑1 (MQ=255)                                                                                                                            tttCCAGGTGAGTAATTTTGCCTCGGCAAAATTAGGCAACAGATAATCGACCTAACTCAATGCTTCGCAAATAGCATCCAGCGTTTCATTCAACCGCGGTTTGATCCTATCGGAACAGATAAGCATCTGCCGGGCTTTAGCGTGCGTaa                        >  2:587477/1‑149 (MQ=255)                                                                                                                                  tgTGAGTAATTTTTCCTCGTCAAAATTAGGAAACAGATAATCGACATAACTAAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAAttt                  <  1:150849/148‑1 (MQ=255)                                                                                                                                   gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAAtttg                 >  2:395147/1‑148 (MQ=255)                                                                                                                                   gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                 <  1:1489971/149‑1 (MQ=255)                                                                                                                                   gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAACCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                 <  1:1197148/149‑1 (MQ=255)                                                                                                                                    ttaGTAATTTTGACTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCt                <  1:449810/147‑1 (MQ=255)                                                                                                                                    tGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCt                <  1:697035/149‑1 (MQ=255)                                                                                                                                     tcctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTg               >  1:316572/4‑149 (MQ=255)                                                                                                                                      cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTGGATCATAACGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGt              >  2:149187/3‑149 (MQ=255)                                                                                                                                        tAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAATAATTTCTGTTa            >  2:1163662/1‑149 (MQ=255)                                                                                                                                        tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCGTGCGTAAAAATTTCTGttt            >  2:836046/1‑148 (MQ=255)                                                                                                                                           ttttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgcg         >  2:1438727/1‑149 (MQ=255)                                                                                                                                               tccTCCGCAAAATTAGGAACCAGATAATCGCCATAACTCAAAGCAGCGCAAATATCATCCAGCGGATCATTCAACCGCGGATTGATCAAATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCttt     <  2:662751/148‑1 (MQ=255)                                                                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTa    <  1:361445/149‑1 (MQ=255)                                                                                                                                                ccTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGGTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTa    <  2:41205/149‑1 (MQ=255)                                                                                                                                                 cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGATTTAGCTTGCGTAAAAATTTCTGTTAGAGCTTTAc   >  2:116705/1‑149 (MQ=255)                                                                                                                                                  tCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGCTAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc  >  2:1366375/1‑149 (MQ=255)                                                                                                                                                  tCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTATGATCATATCGGCACATATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATATCTGTAAGCGCTTTAcc  >  1:1308491/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                     GACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACC  >  NC_000913/1856188‑1856481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑