Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,892,363 A→G 6.4% Y291Y (TAT→TAC)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,892,3630AG 6.4% 230.1 / 5.5 79Y291Y (TAT→TAC) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (26/47);  new base G (2/3);  total (28/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTG  >  NC_000913/1892219‑1892507
                                                                                                                                                |                                                                                                                                                
ggCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAAc                                                                                                                                              <  1:1173519/149‑1 (MQ=255)
  cgcgcTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGGGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTATCGCGGTAACCt                                                                                                                                            <  1:816079/149‑1 (MQ=255)
   gcgcTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGAGTAGCTAACAGCGCACGCAGGTTACCGCGATAACCTg                                                                                                                                           >  2:411627/1‑149 (MQ=255)
    cgcTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTgg                                                                                                                                          <  1:655568/149‑1 (MQ=255)
    cgcTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTgg                                                                                                                                          <  1:448334/149‑1 (MQ=255)
     gcTCAAATGCCGCATCCAGCAAGGCGGACCGCCCCAGTGACAGTTTAGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAAGCTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGc                                                                                                                                         <  2:1568793/149‑1 (MQ=255)
     gcTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTATCGCGGTAACCTgcg                                                                                                                                         <  1:1040008/149‑3 (MQ=255)
      cTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGACTTTGCGGATTAGCTAACAGCTCACGCAGGTTATCGCGGTAACCTgcgt                                                                                                                                        <  1:453776/149‑4 (MQ=255)
         aaaTGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCAc                                                                                                                                     >  1:600777/1‑149 (MQ=255)
           aTGCCGCATCCAGCACGGCGGAACGCCCCAGTGCCCGTTTCGCGACGACATAAGAACGGTCCAGGGTGTCATCGAGCAGGAAATATGCCGGCAGATTGTGGGGATTAGCATACACATCACGCAGGTATCGGCGATAACCGTGCTcccgg                                                                                                                                   >  2:883102/1‑145 (MQ=255)
              ccGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGt                                                                                                                                <  1:523089/149‑1 (MQ=255)
                 cATCCAGCAATGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGc                                                                                                                             <  1:1405176/149‑1 (MQ=255)
                 cATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGc                                                                                                                             <  1:1423556/149‑1 (MQ=255)
                  aTCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGCTCTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCTGATTAGCTAACAGCTCACGCAGGTTACCGCTATAACCTGGCTCAGCGAGTTGCa                                                                                                                            >  1:846126/1‑149 (MQ=255)
                      aGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACTTCATAACAAAGTTCCAGGGTGTCATCGAGCATTAAAAATTCACGCTGAATTTGCGGATTAGCTAACAGCTCAAGCAGGTTACCGAGATAACCTGGCTCCACGAGATCAAGACg                                                                                                                        >  2:1015395/1‑149 (MQ=255)
                      aGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACg                                                                                                                        >  1:835307/1‑149 (MQ=255)
                       gCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGCATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGa                                                                                                                       >  2:255556/1‑149 (MQ=255)
                         aaGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGaaa                                                                                                                     <  2:1206330/149‑1 (MQ=255)
                             cGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATcc                                                                                                                 <  1:413056/149‑1 (MQ=255)
                                aaCGCCCCAGTGACAGTATCGCCACGTCATAACAAAGTTCCACGGTGTCATCGAGCAGTAAAAATGCCCGGTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTgc                                                                                                              <  2:832578/149‑1 (MQ=255)
                                      ccAGTGCCAGTTTCGCCACGTCATAAGATAGTTCCAGGGTGTCATCGAGCAGTAATAATGACCGGTGAATTTGCGGATTAGATAACAGCTCCCGCAGGTTACCGCGATAACCTGGCTCACAGAGTTGCAGCCGAAATTCCTGCGCAgtc                                                                                                        >  2:1052378/1‑149 (MQ=255)
                                       cAGTGACAGTTTCGCCACGTCATATCAAAGTTGCAGGGTGTCATCGAGCAGGAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCAGCGAGTTGCAGACGAAAATCCTGCGCActct                                                                                                       <  1:300000/149‑1 (MQ=255)
                                        aGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTg                                                                                                      <  1:1281707/149‑1 (MQ=255)
                                         gTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAGAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTgg                                                                                                     <  2:216530/149‑1 (MQ=255)
                                         gTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTgg                                                                                                     <  1:963264/149‑1 (MQ=255)
                                          tGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTggg                                                                                                    >  1:429260/1‑149 (MQ=255)
                                          tGACAGTATCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTggg                                                                                                    <  2:1309732/149‑1 (MQ=255)
                                               gTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGa                                                                                               <  2:1287931/149‑1 (MQ=255)
                                                   cGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt                                                                                           <  1:461018/149‑1 (MQ=255)
                                                   cGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGATGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt                                                                                           <  1:941146/149‑1 (MQ=255)
                                                     ccACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCa                                                                                         <  1:827845/149‑1 (MQ=255)
                                                          tCATAACACAGTTCCAGGGTGTCATCGAGCAGTAAAACTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCa                                                                                    <  1:153485/149‑1 (MQ=255)
                                                          tCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCa                                                                                    <  2:451887/149‑1 (MQ=255)
                                                           cATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAc                                                                                   >  1:53118/1‑149 (MQ=255)
                                                             tAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACtt                                                                                 >  2:1102066/1‑149 (MQ=255)
                                                                cAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTg                                                                              <  2:420772/149‑1 (MQ=255)
                                                                 aaaGTTCCAGGGTGTCATCGAGCAGTAAAAAAGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGt                                                                             <  2:1023408/149‑1 (MQ=255)
                                                                    gTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAAc                                                                          <  1:783992/149‑1 (MQ=255)
                                                                    gTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAAc                                                                          <  2:1138718/149‑1 (MQ=255)
                                                                      tCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAActg                                                                        >  2:403637/1‑149 (MQ=255)
                                                                         aGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAActgctg                                                                     >  1:1469084/1‑149 (MQ=255)
                                                                          gggTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCAAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTgg                                                                    <  2:745558/149‑1 (MQ=255)
                                                                              gtCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCTGACGAAAATCCTGCGCACTCTGGGCAAGACGCTCAGCGCACTTTTGTAACTGCTGGgtgt                                                                >  2:146860/1‑149 (MQ=255)
                                                                                cATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCt                                                              >  2:620141/1‑149 (MQ=255)
                                                                                  tCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGCTCAGCGCACTTTTGTAACTGATGGGTGTAttt                                                            >  1:1034759/1‑149 (MQ=255)
                                                                                  tCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCttt                                                            <  2:429260/149‑1 (MQ=255)
                                                                                  tCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCttt                                                            <  2:835307/149‑1 (MQ=255)
                                                                                  tCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCttt                                                            <  2:248607/149‑1 (MQ=255)
                                                                                          tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGACTTTTAATTCgg                                                    >  2:66241/1‑149 (MQ=255)
                                                                                          tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGACTTGCAGACGAAAATCCTGCGCACTATGGTCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCgg                                                    >  2:132096/1‑149 (MQ=255)
                                                                                           aaaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTGACCGAGTTGCAGACGAAAATCCTGCGCACTCTGTGCAAGTCGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTATTTCGGt                                                   <  2:576959/149‑1 (MQ=255)
                                                                                           aaaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTACCTGCTGGGTGTCTTTGAATTCGGt                                                   >  2:634444/1‑149 (MQ=255)
                                                                                            aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTg                                                  <  1:1317794/149‑1 (MQ=255)
                                                                                                  cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAg                                            <  1:623838/149‑1 (MQ=255)
                                                                                                     gCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCg                                         >  2:649183/1‑149 (MQ=255)
                                                                                                         aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATgg                                     <  2:53118/149‑1 (MQ=255)
                                                                                                           tttGCGTATTATCTAACAGCTCACGCATGTTACCGCGATAACCTGGCTCACCGAGTTGCAGAAGAATATCCTGCGCACTCTGTGCAAGACTATCAGCTCACTTTTGTTACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTg                                   <  2:722216/149‑1 (MQ=255)
                                                                                                           tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTg                                   <  1:768465/149‑1 (MQ=255)
                                                                                                           tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGAAAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTg                                   <  2:419177/149‑1 (MQ=255)
                                                                                                             tGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGAt                                 <  2:600777/149‑1 (MQ=255)
                                                                                                             tGCGGATTAGCTAACACCTAACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGAt                                 <  2:365863/149‑1 (MQ=255)
                                                                                                              gCGGATTAGCTAACAGCTCACGCAGGTTACGGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATg                                <  1:1558737/149‑1 (MQ=255)
                                                                                                                 gATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTCCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCt                             >  1:1533835/1‑149 (MQ=255)
                                                                                                                    tAGCTAACAGATCACGCTGGTTACCGCTATATCCTGGCTGACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGTCAAGACGATCAGCTCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTTATGTCtttt                          <  1:1185758/149‑1 (MQ=255)
                                                                                                                       cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGcc                       <  1:497379/149‑1 (MQ=255)
                                                                                                                        tAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCa                      >  1:1427126/1‑149 (MQ=255)
                                                                                                                        tAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGGTGCTGACGAAAATCCTGCGCACTCTGGGCAAGACGATCATCGCACTTTTGTAACTGCTGGGTGTCTGTTAATTCGGTGCGGTAGGCGATGGTGAGTTCTTTTTCCa                      >  1:1127237/1‑149 (MQ=255)
                                                                                                                         aaCAGCTCACGCAGGTTACCGCGATAACCTGGCTCCCCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAg                     >  1:952887/1‑149 (MQ=255)
                                                                                                                           cAGCTAACGCAGGTTTCCGCGATAACCTGTCTCACCGAGTTGCAGTCGAAACTCCTGCGCACTCTGGGAAAGATGATCAGCGCACTTTTTTAACTGCTTGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTTATGTCTTTTGACATGt                   <  2:846126/149‑1 (MQ=255)
                                                                                                                            cgCTCACGCAGGCTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTc                  <  2:1551713/148‑1 (MQ=255)
                                                                                                                              cTCACGAAGTGTACCGCGATTACCTGGCTCACCGAGCTGCAGCCGAAACTCCTGCGCACTCTGTGCAAGACGTTCAGCGCACTTTTGTACCTGCTGGGTGGCTTTTAATTCTGTGCGGTAGGCGATGGTGATGGCTTTTGCCAGGTCGa                <  2:771191/149‑1 (MQ=255)
                                                                                                                               tCACGCACTTTACCGCGTTAACCTGGCTCACCTAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGCTCAGCGCACTTTTGTAACTGCTGGGTTTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAg               <  1:1084330/149‑1 (MQ=255)
                                                                                                                                 agccAGGTTATCGCGGTAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGGACTTTTGTAACTGCTGGGTGTCTGTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGACAGGTCGAgga             >  1:1262096/4‑147 (MQ=255)
                                                                                                                                 aCGCAGGTTACTGAGATAACCTTGCTCACCGATTTGCAGACTAAAATACTGCGCACTCTGGGCAAGTCGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTTATTCGGTGCGGTAGGGGATGGTGATGTCTTTTGCCAGGTGGTGCa             <  1:1015395/149‑1 (MQ=255)
                                                                                                                                 aCGCAGGTTACCGCGATAACCTGGCTCCCCGAGTTCCAGACGAAAATCCTGCGCACTCTGGTCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGAGGTGGATGTCTTTTGCCAGGTCGAGCa             >  1:818927/1‑149 (MQ=255)
                                                                                                                                  gccAGGTTATCGCGGTAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAg            >  1:176916/3‑149 (MQ=255)
                                                                                                                                      ggTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTATGGGCAAGACGATCAGCGCACTTTTGTTACTGCTGGGTGTCTGATAATTATGTGAGGTAGGCGATGGTGATGTATTTTGCCAGGTCGAGAAGTTGt        >  1:1073459/1‑149 (MQ=255)
                                                                                                                                           ccGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGTGTGTCTTTTATTTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACt   <  2:1469084/149‑1 (MQ=255)
                                                                                                                                            cgcgATAACCTGGCTCACCGAGTTGCAGACGAAAATACTGCGAACTCTGTGCATGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTg  <  2:1541072/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGCGCGCTCAAATGCCGCATCCAGCAAGGCGGAACGCCCCAGTGACAGTTTCGCCACGTCATAACAAAGTTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCACTTTTGTAACTGCTGGGTGTCTTTTAATTCGGTGCGGTAGGCGATGGTGATGTCTTTTGCCAGGTCGAGCAGTTGTCGACTG  >  NC_000913/1892219‑1892507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑