Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,906,229 T→G 5.3% I181S (ATC→AGC)  mntP → putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,906,2290TG 5.3% 292.2 / 4.4 110I181S (ATC→AGC) mntPputative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base T (62/42);  new base G (4/2);  total (66/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

Only 100 of 111 total aligned reads displayed.

AACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATG  >  NC_000913/1906081‑1906375
                                                                                                                                                    |                                                                                                                                                  
 aCATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGATAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATc                                                                                                                                                   >  2:1483528/1‑149 (MQ=255)
  cATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCGTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTGTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGTGCTGGTGATGCTCGGCATCGGCGTCCAGATca                                                                                                                                                  >  2:482586/1‑148 (MQ=255)
    ttATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGAGTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctc                                                                                                                                                >  1:908573/1‑149 (MQ=255)
     tATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCctct                                                                                                                                               >  1:457745/1‑149 (MQ=255)
        cgcgACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGa                                                                                                                                            <  1:15415/149‑1 (MQ=255)
           caCCGCATTGGCCAGTGGTTGTGCAACCTTGATAATGTCAACATTAGGGATGATGGTAGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGGATCTGGACGc                                                                                                                                         <  1:201614/148‑1 (MQ=255)
             ccGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCAc                                                                                                                                       <  2:432435/149‑1 (MQ=255)
               ccATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAACTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACtt                                                                                                                                     <  1:56897/148‑1 (MQ=255)
                 aTTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTcc                                                                                                                                   >  2:699374/1‑149 (MQ=255)
                    ggCCTTTGGTTGTGCAACCTTGATTATGTCTAATTAAGGGATGATGGTTGGTCGGTTTATCGGCTCTATTTTTGGCATAAAAGCGGAAATTCTCGGCGGGCTGGAGCTGATCGGCATCGGGGTCCAGATCCTCTGTACGCACTTCCACg                                                                                                                                <  2:1336431/149‑1 (MQ=255)
                      ccATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGt                                                                                                                              >  2:1283732/1‑149 (MQ=255)
                       cATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGtt                                                                                                                             >  1:1175043/1‑149 (MQ=255)
                        aTTGGTTGTGCAACCGTGATTATGGCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTta                                                                                                                            <  2:744523/149‑1 (MQ=255)
                            gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTtaataa                                                                                                                        >  1:678953/1‑149 (MQ=255)
                            gTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGGtaatca                                                                                                                        >  1:748080/1‑147 (MQ=255)
                             ttGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                       <  2:1491690/149‑1 (MQ=255)
                             ttGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAg                                                                                                                       >  2:1299589/1‑149 (MQ=255)
                             gggggCAGCCTTGAGTATCTCTCCATTACGGATGTTGGTTGGGCTCTTTATCGTCTCAAGTTATGGGAAACACGCGGACATGCTCGCCGGGCGGGTGCTGATCGGCATCGGCGTCCAGCTCCTCTGGACGGACTTCCACGGTttatatg                                                                                                                       <  2:1447828/145‑3 (MQ=255)
                               gtgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCAGCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGaa                                                                                                                     <  1:792050/149‑1 (MQ=255)
                                tgCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCATATCCTCTGGACGCACTTCCACGGTTAATAAGAAc                                                                                                                    >  1:396200/1‑149 (MQ=255)
                                 gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGTGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACg                                                                                                                   >  1:789835/1‑149 (MQ=255)
                                 gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACg                                                                                                                   >  2:1288468/1‑149 (MQ=255)
                                 gCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGCAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCATCGGGACGCACTTCCCCGGTAAATAAGAACg                                                                                                                   >  2:1053007/1‑149 (MQ=255)
                                    aCCATGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTg                                                                                                                >  2:952121/1‑149 (MQ=255)
                                     ccTTGATTATGTCAACATTAGGGATGATGGTTGGGCGCGTTATCGGCTCAATTATTGGCAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGc                                                                                                               <  1:184933/149‑1 (MQ=255)
                                      cTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTCGGAAAAACGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGGTAATACGAACGCTGcc                                                                                                              <  2:646866/149‑1 (MQ=255)
                                       ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTTGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                             >  1:143753/1‑149 (MQ=255)
                                       ttGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCa                                                                                                             >  1:352919/1‑149 (MQ=255)
                                         gcttATGCCTACATTAGGTAGGATGCTTGGTCGCTTTATTGTCTCAATTATTGGGAAAAAAGCGGAATCTCTTGCCGGGCTGGTGCTGAGCGGCATCGGCGTCCAGATCCGCTGGACGCAGTTCCACGGTTAATAAGAACGCTGCCaga                                                                                                           <  2:1427071/147‑1 (MQ=255)
                                          aTTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATTAGAACGCTGCCagag                                                                                                          >  1:174738/1‑149 (MQ=255)
                                            tATGTGACCCTTATGTATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGTTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGt                                                                                                        <  1:718516/149‑1 (MQ=255)
                                              tGTCAACATTAGGGATGATGGTTGGGCGCTTTATCGGCTCATTTATTGGGAAAAACGCGGAAATTCTCGGCGGGCGGGTGCTGATCGGCAGCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGa                                                                                                      <  1:850442/149‑1 (MQ=255)
                                               gTCATCATTAGGGATTCTGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCGGGTGCTTAGCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                     <  2:1525128/149‑1 (MQ=255)
                                               gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                     >  2:1052184/1‑149 (MQ=255)
                                               gTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGaa                                                                                                     >  1:165247/1‑149 (MQ=255)
                                                 cAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCGGCCAGAGGTGAata                                                                                                   >  1:1027867/1‑149 (MQ=255)
                                                   aCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCCCGGTTAATAAGAACGCTGCCAGAGGTGAATAtt                                                                                                 >  2:89939/1‑149 (MQ=255)
                                                   aCATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGatgatt                                                                                                 >  2:1549518/1‑144 (MQ=255)
                                                      ttAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCTGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCTGCGTCCAGATCCTATGGACGCACTTCCACGGGTAATAAGAACGCTGGCAGACGTGAATTTTaaa                                                                                              >  2:1083715/1‑149 (MQ=255)
                                                        aGGGATGATGGTTGGGCTCTTTATCGGCGCAATTATTGGGAAAAAAGCTGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGt                                                                                            <  1:1131053/149‑1 (MQ=255)
                                                          ggATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTcc                                                                                          >  2:200954/1‑149 (MQ=255)
                                                             tgatgGTTGGGCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTc                                                                                       <  2:69018/149‑1 (MQ=255)
                                                               atgGTTGGTCGCTTTATCTGCTAAATTATTGGGAAAAAAGCGGAAATTCTCGGGGGTCTGGTGCTGATCTGCATCGGCTTCCAGATCCTCTGGACGCACTTCCACGGTTAATAATAACGCTGCCAGAGGAGAATATTAAAGTCAGTCTg                                                                                     >  2:74303/1‑149 (MQ=255)
                                                                 ggttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                   >  2:900279/1‑149 (MQ=255)
                                                                 ggttggtCGCTTTATAGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGATGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGTACGCACTTCCACGGTTAATAAGAACGCTGCAAGAGGTGAATATTAAAGTCCGTCTGGc                                                                                   >  2:132244/1‑149 (MQ=255)
                                                                  gttggtCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGATTATTAAAGTCCGTCTGGCa                                                                                  >  2:902727/1‑149 (MQ=255)
                                                                  gttggtCGCTTTATCGGCTAAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCa                                                                                  <  1:485198/149‑1 (MQ=255)
                                                                     gggcGCTTTCTCGGCTCAATTAAGGCGAAAAAAGCGGACATTCCCGGCGGGCTGGTGCTGCGCGGCATCGGCGGCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTc                                                                               <  2:1007707/146‑1 (MQ=255)
                                                                        cGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGaa                                                                            <  2:678953/149‑1 (MQ=255)
                                                                              aTCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACTCACTTCCACGGTTAATAAGAACGCTGCCATAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActtc                                                                      >  1:536635/1‑149 (MQ=255)
                                                                               tCGGCTCAATAATGTGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGAGCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTACTAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActtct                                                                     <  2:1332673/149‑1 (MQ=255)
                                                                                cgcctgaaTTATTGGGAAAAATGCGGAAATTCTCGGCGGGCTGGTGCTTATCGGCATCGGGGTCCAGTTCGTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACActtctt                                                                    <  1:1416957/143‑1 (MQ=255)
                                                                                 ggCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCttt                                                                   <  2:278842/149‑1 (MQ=255)
                                                                                   cTCAAATATTGTGAAAAAAGAGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTACAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtg                                                                 <  2:1027867/149‑1 (MQ=255)
                                                                                    tCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCGTCTGGACGCACTTCCACGGTTTATAAGAACGCTGCCAGAGGTGAATATTTAAGTCCGTCTGGTTGTGGAACACTTCTTTtgc                                                                >  2:123851/1‑149 (MQ=255)
                                                                                    tCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACCCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgc                                                                >  1:805184/1‑149 (MQ=255)
                                                                                     cAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGCCCGTCTGGCAGGCGAACACTTCTTTtgct                                                               <  1:740031/149‑1 (MQ=255)
                                                                                       attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctgc                                                             <  1:699374/149‑1 (MQ=255)
                                                                                       attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctgc                                                             <  1:1299589/149‑1 (MQ=255)
                                                                                       attattGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTtgctgc                                                             >  2:978460/1‑149 (MQ=255)
                                                                                         tattGCGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGAGCCTCTGGACGCACGTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCa                                                           <  2:1493373/149‑1 (MQ=255)
                                                                                              ggAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGGAAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGtt                                                      >  1:1098060/1‑149 (MQ=255)
                                                                                              ggAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGCGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGtt                                                      >  1:1103859/1‑149 (MQ=255)
                                                                                              ggAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGATAATAAGAACGCTGCCAGAGGTGAATATTTAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTTCAAGTGtt                                                      >  2:914278/1‑149 (MQ=255)
                                                                                                aaaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGCCGCACGTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCGGGCAGTCGACCACTTCTATAGCTGCCAGAGTTTg                                                    >  1:1534517/1‑149 (MQ=255)
                                                                                                 aaaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGc                                                   >  1:582591/1‑149 (MQ=255)
                                                                                                  aaaaGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGcc                                                  >  2:214429/1‑149 (MQ=255)
                                                                                                     aGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGa                                               <  2:593902/149‑1 (MQ=255)
                                                                                                       cGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACt                                             >  1:810586/1‑149 (MQ=255)
                                                                                                        ggAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACtt                                            <  1:1483528/149‑1 (MQ=255)
                                                                                                          aaaTTCTCGGCGGGCTGGTGCTGATCGTCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                          <  2:93065/149‑1 (MQ=255)
                                                                                                          aaaTTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGCCGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCt                                          >  1:903105/1‑149 (MQ=255)
                                                                                                                    cggGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATACGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCAc                                <  2:1248084/149‑1 (MQ=255)
                                                                                                                         tGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAg                           <  1:200954/149‑1 (MQ=255)
                                                                                                                           gTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCAAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                         >  2:841768/1‑149 (MQ=255)
                                                                                                                           gTGCGGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGGCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGc                         <  2:1303910/149‑1 (MQ=255)
                                                                                                                             gCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCaa                       >  2:147806/1‑149 (MQ=255)
                                                                                                                               tGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAAc                     >  2:470514/1‑149 (MQ=255)
                                                                                                                                gATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACg                    >  1:678271/1‑149 (MQ=255)
                                                                                                                                 atcggcatcggcGTCCAGATCCTCTGGACGCACTTCCCCGGTTAATAAGAACGCTGCCAGCGGTGAAGATTAAAGTCCGTCTCGCAGTCGCACACTTCTTTTGCTGCCAGTGTTTTCCAGACTTCTGTCTTCGCACGCCAGGCAAACgg                   >  1:862328/1‑149 (MQ=255)
                                                                                                                                  tcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGACCACTTCTTTTGCAGCCAGTGTTTGCCAGACTTCTGCCTTCGCACGCGAGGCAAACGGc                  >  1:806321/1‑149 (MQ=255)
                                                                                                                                  tcggcatcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGc                  >  1:813015/1‑149 (MQ=255)
                                                                                                                                      catcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCa              >  2:293652/1‑149 (MQ=255)
                                                                                                                                        tcggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATc            <  2:91226/149‑1 (MQ=255)
                                                                                                                                         gtgcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCt           >  1:695615/3‑149 (MQ=255)
                                                                                                                                          tgcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCTGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTg          >  1:522964/2‑149 (MQ=255)
                                                                                                                                          ggcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTg          <  2:1175043/149‑1 (MQ=255)
                                                                                                                                           gcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGCCTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc         >  1:34838/1‑149 (MQ=255)
                                                                                                                                           gcGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc         <  1:1283732/149‑1 (MQ=255)
                                                                                                                                           gcGTCCAGATCCTATGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCAAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc         <  2:709237/149‑1 (MQ=255)
                                                                                                                                           gcGTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGc         >  2:309691/1‑149 (MQ=255)
                                                                                                                                             gTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaa       <  1:762199/149‑1 (MQ=255)
                                                                                                                                             gTCCAGAGGATCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaa       >  2:1542668/1‑149 (MQ=255)
                                                                                                                                               ccAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGATGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGGCATCTGCaata     >  2:1389680/1‑149 (MQ=255)
                                                                                                                                                cAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAACCGGCGTCATCTGCaataa    >  2:382820/1‑149 (MQ=255)
                                                                                                                                                cAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACTGCGGCATCTGCaataa    >  2:1075244/1‑149 (MQ=255)
                                                                                                                                                cAGATCCTCTGGACGCACTTCCACGGTGAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCCCGCCAGGCAAACGGCGTCATCTGCaataa    >  2:238660/1‑149 (MQ=255)
                                                                                                                                                cAGATACTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTAAGCACTCCAGGCAAACGGGGTAATCTGAaataa    >  1:1021927/1‑149 (MQ=255)
                                                                                                                                                 aGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCaataat   >  1:217612/1‑149 (MQ=255)
                                                                                                                                                  gATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATg  <  2:9939/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCTCAATTATTGGGAAAAAAGCGGAAATTCTCGGCGGGCTGGTGCTGATCGGCATCGGCGTCCAGATCCTCTGGACGCACTTCCACGGTTAATAAGAACGCTGCCAGAGGTGAATATTAAAGTCCGTCTGGCAGTCGAACACTTCTTTTGCTGCCAGTGTTTGCCAGACTTCTGGCTTCGCACGCCAGGCAAACGGCGTCATCTGCAATAATG  >  NC_000913/1906081‑1906375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: