Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,996,373 T→A 6.3% M154L (ATG→TTG)  sdiA ← quorum‑sensing transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,3730TA 6.3% 232.1 / 3.3 84M154L (ATG→TTG) sdiAquorum‑sensing transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (37/41);  new base A (2/4);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAC  >  NC_000913/1996227‑1996518
                                                                                                                                                  |                                                                                                                                                 
catcGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAttt                                                                                                                                                 <  2:668092/149‑1 (MQ=255)
    gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGATTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGAGTGATCCCTATTTCATCATTTAAACGAATCTTAACCATCAGACTTTCGCGCCCCAGTAACTGCATTTATaa                                                                                                                                             >  1:872608/1‑149 (MQ=255)
    gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                             <  2:1106674/149‑1 (MQ=255)
    gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                             >  1:209552/1‑149 (MQ=255)
    gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTATTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                             <  2:1100969/149‑1 (MQ=255)
     cTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAAt                                                                                                                                            >  1:589306/1‑149 (MQ=255)
     cTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCAACAGACTTTCGCGCACCAGTAACTGCAattaaaaa                                                                                                                                            >  2:877774/1‑144 (MQ=255)
          tctGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg                                                                                                                                       <  2:1216495/149‑1 (MQ=255)
           ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGt                                                                                                                                      >  1:807855/1‑149 (MQ=255)
           ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGt                                                                                                                                      <  1:877774/149‑1 (MQ=255)
            tgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTACGCGCACCAGTAACTGCAATTAAAAATGCAGtt                                                                                                                                     <  1:455141/149‑1 (MQ=255)
             gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtc                                                                                                                                    <  1:1553363/149‑1 (MQ=255)
             gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTAATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtga                                                                                                                                    >  2:168571/1‑147 (MQ=255)
              ctgATGTTTTCCGTTCCGCCGTCCACCTCAGATTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCTCTAATTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGttac                                                                                                                                   <  2:910146/149‑3 (MQ=255)
              ctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtca                                                                                                                                   >  1:1406752/1‑149 (MQ=255)
               tgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcat                                                                                                                                  <  2:1347388/149‑1 (MQ=255)
               ggATGTTTTCCCTTACGCCGTCCACCTCAGAAGTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATGATTTAATCTCATCAGAGCCATCAGTCTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcat                                                                                                                                  <  1:1058131/148‑1 (MQ=255)
                gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcatc                                                                                                                                 >  1:436521/1‑149 (MQ=255)
                 aTGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcatca                                                                                                                                >  2:54836/1‑149 (MQ=255)
                      ttCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAg                                                                                                                           <  2:1492388/149‑1 (MQ=255)
                         ccatCCGCAGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTAATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAAt                                                                                                                        <  1:153496/146‑1 (MQ=255)
                         ccTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCCTCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAAt                                                                                                                        >  1:39621/1‑149 (MQ=255)
                          cTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATg                                                                                                                       >  1:267819/1‑149 (MQ=255)
                           ttCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATgg                                                                                                                      <  1:443905/149‑1 (MQ=255)
                                   tCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTAAAACGCATCAGAGCCTTCAGACTTTCGCGCACAAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcg                                                                                                              >  1:1100130/1‑149 (MQ=255)
                                   gccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCACTGGCGTCATCAGTATTTCATCATTTAAACGCATCAGAGGCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTAtttag                                                                                                              >  2:124435/2‑147 (MQ=255)
                                    ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                             >  1:1272988/1‑149 (MQ=255)
                                    ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                             <  2:558498/149‑1 (MQ=255)
                                    ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                             <  2:1402579/149‑1 (MQ=255)
                                     cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAATAATGGGTATTTagcg                                                                                                            >  2:193931/1‑149 (MQ=255)
                                     cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcg                                                                                                            >  2:603391/1‑149 (MQ=255)
                                      aCCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgc                                                                                                           >  1:1331519/1‑149 (MQ=255)
                                         tCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg                                                                                                        >  1:808751/1‑149 (MQ=255)
                                          cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTACACGCTTCAGAGCCATCAGACTTTCGCGGCCCAGTATCTGCATTTTTAATTGCTGTTGGTCTCGTAGAATGGGTATTTCgcgcgcgc                                                                                                       >  2:487145/1‑149 (MQ=255)
                                          cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCgcgcgcgc                                                                                                       <  1:584034/149‑1 (MQ=255)
                                           aGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCt                                                                                                      <  2:68296/149‑1 (MQ=255)
                                           aGAATTTCGTTATCGCGCTTGCTGAAATTCATCTCTGGCGGCCTCACTATTTCATCATTTACACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTAGTTCGCGCGCGCt                                                                                                      <  1:1411598/149‑1 (MQ=255)
                                             aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGc                                                                                                    <  1:683712/149‑1 (MQ=255)
                                                ttCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAAc                                                                                                 <  1:1287099/149‑1 (MQ=255)
                                                     tttCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGaa                                                                                            <  1:211057/149‑1 (MQ=255)
                                                             ttGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACaa                                                                                    <  2:322883/149‑1 (MQ=255)
                                                               tcTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACaaaa                                                                                  <  1:752234/148‑1 (MQ=255)
                                                                 ttataTTCTTCTGTGGTTTAATCACTTTTTCTTATTTTAATCGCATCAGAGCCATCAGACTTTGGATATCGAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGGGCGCTGCAACGGTAAATGGACAAAAAg                                                                                <  2:1044588/145‑1 (MQ=255)
                                                                  gAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGc                                                                               >  1:948529/1‑149 (MQ=255)
                                                                   aaaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGGAACGGGAAAAGGACAAAACGcc                                                                              >  2:968197/1‑149 (MQ=255)
                                                                   aaaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACTAAAAGcc                                                                              <  2:92932/149‑1 (MQ=255)
                                                                     aTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGcccc                                                                            >  2:433737/1‑148 (MQ=255)
                                                                     aTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCa                                                                            <  1:1241632/149‑1 (MQ=255)
                                                                           ctctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGccc                                                                      >  2:68954/1‑149 (MQ=255)
                                                                             ctGGCGTCATCACTATTTCATCATTTAACCGCATCAGAGCCATCAGACTTTCGCGCCCCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAACGCCCAGCGCCCgg                                                                    >  1:890312/1‑149 (MQ=255)
                                                                             ctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCgg                                                                    >  1:776939/1‑149 (MQ=255)
                                                                              tGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCTCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGt                                                                   >  1:523714/1‑149 (MQ=255)
                                                                                  gTCATAACTATTTCTTCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTgg                                                               <  1:1186599/149‑1 (MQ=255)
                                                                                         ctctttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGGTTGGCAGCAtt                                                        <  2:464960/146‑1 (MQ=255)
                                                                                         cTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAtt                                                        >  2:84822/1‑149 (MQ=255)
                                                                                          tataTCATCATTTACACGCATCAGAGCCATCAGACTTTCGCGCACGAGTACCTGCATTTTACATTGCAGTTCATCACTAACAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTa                                                       <  1:1147534/149‑1 (MQ=255)
                                                                                                atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCTCTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                 <  1:1187296/149‑1 (MQ=255)
                                                                                                atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                 >  1:401693/1‑149 (MQ=255)
                                                                                                atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                 <  1:1567358/149‑1 (MQ=255)
                                                                                                atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGGTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAACGACAAAAAGCCCAGCGACCGGTATGGCAGCATAAAAAAca                                                 >  1:875232/1‑148 (MQ=255)
                                                                                                  catTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGa                                               <  2:589306/149‑1 (MQ=255)
                                                                                                  catTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATATTTAATTGCAGTTCATCACTAAGAATGGGGATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGa                                               <  1:1563976/149‑1 (MQ=255)
                                                                                                   ttttAAACGCATCAGAGCCATCAGTCTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCTCTAAGATTGGGTATTTCGCGCGCGCGGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                              <  1:1442232/148‑1 (MQ=255)
                                                                                                   atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                              >  1:584578/1‑149 (MQ=255)
                                                                                                   atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                              <  1:54836/149‑1 (MQ=255)
                                                                                                    tttAAACGCATCAGAGCCATCAGAGTTTCGCGCAGGAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGTGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                             <  1:124435/149‑1 (MQ=255)
                                                                                                    tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAGTAAATACTGAGt                                             >  1:496920/1‑149 (MQ=255)
                                                                                                       aaaCGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAACTACTGAGTGac                                          >  2:488644/1‑149 (MQ=255)
                                                                                                        aaCGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGaca                                         >  1:319770/1‑149 (MQ=255)
                                                                                                               cggagCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCgcggc                                  >  2:663784/3‑149 (MQ=255)
                                                                                                                    ccATCAGACTTTCTCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTaaa                             <  2:267819/149‑1 (MQ=255)
                                                                                                                        cAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAt                         <  2:1406752/149‑1 (MQ=255)
                                                                                                                        cAGACTTTCGCGCACCAGTAACTGCATGTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAt                         <  2:523481/149‑1 (MQ=255)
                                                                                                                         aGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtg                        <  2:451692/149‑1 (MQ=255)
                                                                                                                         aGACATTCGAGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCTACGGGAAAAGTACAAAAAGCCCTGCGCCCTGTTTGGCAGGATTAAATACTGAGTGACACCGCGGCGTGAGCCAtg                        >  2:293705/1‑149 (MQ=255)
                                                                                                                           aCTTTCGCGCACCAGTAACTGCAATTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGAGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtgtg                      >  2:822403/1‑149 (MQ=255)
                                                                                                                             tttCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCTCGGCGTAAACCATGTgcg                    >  1:189582/1‑149 (MQ=255)
                                                                                                                                 gcgcACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTgcgcgcg                >  1:70073/1‑149 (MQ=255)
                                                                                                                                  cgcACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTgcgcgcgc               <  1:1438919/149‑1 (MQ=255)
                                                                                                                                    cACCAGTAACAGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCgg             <  2:1556844/149‑1 (MQ=255)
                                                                                                                                      ccAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAACAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCt           <  1:448640/149‑1 (MQ=255)
                                                                                                                                        attAACTGCAATTTTAATTGCAGTTCATCACTAAGAATGTGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTc         <  1:968197/147‑1 (MQ=255)
                                                                                                                                          tAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTccc       >  1:553432/1‑149 (MQ=255)
                                                                                                                                          tAACAGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTCTTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTccc       <  2:1238134/149‑1 (MQ=255)
                                                                                                                                          gaaCTGCAATTAAAAATGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGTACAAAAAGCCCAGCGACCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTcac       >  1:124874/2‑147 (MQ=255)
                                                                                                                                               gCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAc  <  1:603391/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAC  >  NC_000913/1996227‑1996518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: