Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,996,376 T→A 5.4% K153* (AAA→TAA)  sdiA ← quorum‑sensing transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,3760TA 5.4% 233.4 / 2.2 84K153* (AAA→TAA) sdiAquorum‑sensing transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (37/42);  new base A (2/3);  total (39/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGT  >  NC_000913/1996231‑1996524
                                                                                                                                                 |                                                                                                                                                    
gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGATTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGAGTGATCCCTATTTCATCATTTAAACGAATCTTAACCATCAGACTTTCGCGCCCCAGTAACTGCATTTATaa                                                                                                                                                   >  1:872608/1‑149 (MQ=255)
gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                                   >  1:209552/1‑149 (MQ=255)
gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                                   <  2:1106674/149‑1 (MQ=255)
gCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTATTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                                                                                                                                   <  2:1100969/149‑1 (MQ=255)
 cTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAAt                                                                                                                                                  >  1:589306/1‑149 (MQ=255)
      tctGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAg                                                                                                                                             <  2:1216495/149‑1 (MQ=255)
       ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGt                                                                                                                                            >  1:807855/1‑149 (MQ=255)
       ctgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGt                                                                                                                                            <  1:877774/149‑1 (MQ=255)
        tgctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTACGCGCACCAGTAACTGCAATTAAAAATGCAGtt                                                                                                                                           <  1:455141/149‑1 (MQ=255)
         gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtc                                                                                                                                          <  1:1553363/149‑1 (MQ=255)
         gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTAATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtga                                                                                                                                          >  2:168571/1‑147 (MQ=255)
          ctgATGTTTTCCGTTCCGCCGTCCACCTCAGATTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCTCTAATTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCAATTAAAAATGCAGttac                                                                                                                                         <  2:910146/149‑3 (MQ=255)
          ctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtca                                                                                                                                         >  1:1406752/1‑149 (MQ=255)
           tgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcat                                                                                                                                        <  2:1347388/149‑1 (MQ=255)
           ggATGTTTTCCCTTACGCCGTCCACCTCAGAAGTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATGATTTAATCTCATCAGAGCCATCAGTCTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcat                                                                                                                                        <  1:1058131/148‑1 (MQ=255)
            gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcatc                                                                                                                                       >  1:436521/1‑149 (MQ=255)
             aTGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtcatca                                                                                                                                      >  2:54836/1‑149 (MQ=255)
                  ttCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAg                                                                                                                                 <  2:1492388/149‑1 (MQ=255)
                     ccatCCGCAGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTAATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAAt                                                                                                                              <  1:153496/146‑1 (MQ=255)
                     ccTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCCTCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAAt                                                                                                                              >  1:39621/1‑149 (MQ=255)
                      cTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATg                                                                                                                             >  1:267819/1‑149 (MQ=255)
                       ttCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATgg                                                                                                                            <  1:443905/149‑1 (MQ=255)
                               tCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTAAAACGCATCAGAGCCTTCAGACTTTCGCGCACAAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcg                                                                                                                    >  1:1100130/1‑149 (MQ=255)
                               gccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCACTGGCGTCATCAGTATTTCATCATTTAAACGCATCAGAGGCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTAtttag                                                                                                                    >  2:124435/2‑147 (MQ=255)
                                ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                                   <  2:558498/149‑1 (MQ=255)
                                ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                                   <  2:1402579/149‑1 (MQ=255)
                                ccACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgc                                                                                                                   >  1:1272988/1‑149 (MQ=255)
                                 cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAATAATGGGTATTTagcg                                                                                                                  >  2:193931/1‑149 (MQ=255)
                                 cACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcg                                                                                                                  >  2:603391/1‑149 (MQ=255)
                                  aCCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgc                                                                                                                 >  1:1331519/1‑149 (MQ=255)
                                     tCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg                                                                                                              >  1:808751/1‑149 (MQ=255)
                                      cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTACACGCTTCAGAGCCATCAGACTTTCGCGGCCCAGTATCTGCATTTTTAATTGCTGTTGGTCTCGTAGAATGGGTATTTCgcgcgcgc                                                                                                             >  2:487145/1‑149 (MQ=255)
                                      cAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCgcgcgcgc                                                                                                             <  1:584034/149‑1 (MQ=255)
                                       aGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCt                                                                                                            <  2:68296/149‑1 (MQ=255)
                                       aGAATTTCGTTATCGCGCTTGCTGAAATTCATCTCTGGCGGCCTCACTATTTCATCATTTACACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTAGTTCGCGCGCGCt                                                                                                            <  1:1411598/149‑1 (MQ=255)
                                         aaTTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGc                                                                                                          <  1:683712/149‑1 (MQ=255)
                                            ttCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAAc                                                                                                       <  1:1287099/149‑1 (MQ=255)
                                                 tttCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGaa                                                                                                  <  1:211057/149‑1 (MQ=255)
                                                         ttGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACaa                                                                                          <  2:322883/149‑1 (MQ=255)
                                                           tcTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACaaaa                                                                                        <  1:752234/148‑1 (MQ=255)
                                                             ttataTTCTTCTGTGGTTTAATCACTTTTTCTTATTTTAATCGCATCAGAGCCATCAGACTTTGGATATCGAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGGGCGCTGCAACGGTAAATGGACAAAAAg                                                                                      <  2:1044588/145‑1 (MQ=255)
                                                              gAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGc                                                                                     >  1:948529/1‑149 (MQ=255)
                                                               aaaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGGAACGGGAAAAGGACAAAACGcc                                                                                    >  2:968197/1‑149 (MQ=255)
                                                               aaaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACTAAAAGcc                                                                                    <  2:92932/149‑1 (MQ=255)
                                                                 aTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGcccc                                                                                  >  2:433737/1‑148 (MQ=255)
                                                                 aTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCa                                                                                  <  1:1241632/149‑1 (MQ=255)
                                                                       ctctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGccc                                                                            >  2:68954/1‑149 (MQ=255)
                                                                         ctGGCGTCATCACTATTTCATCATTTAACCGCATCAGAGCCATCAGACTTTCGCGCCCCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAACGCCCAGCGCCCgg                                                                          >  1:890312/1‑149 (MQ=255)
                                                                         ctGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCgg                                                                          >  1:776939/1‑149 (MQ=255)
                                                                          tGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCTCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGt                                                                         >  1:523714/1‑149 (MQ=255)
                                                                              gTCATAACTATTTCTTCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTgg                                                                     <  1:1186599/149‑1 (MQ=255)
                                                                                     ctctttCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGGTTGGCAGCAtt                                                              <  2:464960/146‑1 (MQ=255)
                                                                                     cTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAtt                                                              >  2:84822/1‑149 (MQ=255)
                                                                                      tataTCATCATTTACACGCATCAGAGCCATCAGACTTTCGCGCACGAGTACCTGCATTTTACATTGCAGTTCATCACTAACAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTa                                                             <  1:1147534/149‑1 (MQ=255)
                                                                                            atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCTCTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                       <  1:1187296/149‑1 (MQ=255)
                                                                                            atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                       >  1:401693/1‑149 (MQ=255)
                                                                                            atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACt                                                       <  1:1567358/149‑1 (MQ=255)
                                                                                            atcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGGTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAACGACAAAAAGCCCAGCGACCGGTATGGCAGCATAAAAAAca                                                       >  1:875232/1‑148 (MQ=255)
                                                                                              catTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGa                                                     <  2:589306/149‑1 (MQ=255)
                                                                                              catTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATATTTAATTGCAGTTCATCACTAAGAATGGGGATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGa                                                     <  1:1563976/149‑1 (MQ=255)
                                                                                               ttttAAACGCATCAGAGCCATCAGTCTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCTCTAAGATTGGGTATTTCGCGCGCGCGGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                                    <  1:1442232/148‑1 (MQ=255)
                                                                                               atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                                    >  1:584578/1‑149 (MQ=255)
                                                                                               atTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAg                                                    <  1:54836/149‑1 (MQ=255)
                                                                                                tttAAACGCATCAGAGCCATCAGAGTTTCGCGCAGGAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGTGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGt                                                   <  1:124435/149‑1 (MQ=255)
                                                                                                tttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCAGTAAATACTGAGt                                                   >  1:496920/1‑149 (MQ=255)
                                                                                                   aaaCGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAACTACTGAGTGac                                                >  2:488644/1‑149 (MQ=255)
                                                                                                    aaCGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGaca                                               >  1:319770/1‑149 (MQ=255)
                                                                                                           cggagCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCgcggc                                        >  2:663784/3‑149 (MQ=255)
                                                                                                                ccATCAGACTTTCTCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTaaa                                   <  2:267819/149‑1 (MQ=255)
                                                                                                                    cAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAt                               <  2:1406752/149‑1 (MQ=255)
                                                                                                                    cAGACTTTCGCGCACCAGTAACTGCATGTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAt                               <  2:523481/149‑1 (MQ=255)
                                                                                                                     aGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtg                              <  2:451692/149‑1 (MQ=255)
                                                                                                                     aGACATTCGAGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCTACGGGAAAAGTACAAAAAGCCCTGCGCCCTGTTTGGCAGGATTAAATACTGAGTGACACCGCGGCGTGAGCCAtg                              >  2:293705/1‑149 (MQ=255)
                                                                                                                       aCTTTCGCGCACCAGTAACTGCAATTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGAGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCAtgtg                            >  2:822403/1‑149 (MQ=255)
                                                                                                                         tttCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCTCGGCGTAAACCATGTgcg                          >  1:189582/1‑149 (MQ=255)
                                                                                                                             gcgcACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTgcgcgcg                      >  1:70073/1‑149 (MQ=255)
                                                                                                                              cgcACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTgcgcgcgc                     <  1:1438919/149‑1 (MQ=255)
                                                                                                                                cACCAGTAACAGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCgg                   <  2:1556844/149‑1 (MQ=255)
                                                                                                                                  ccAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAACAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCt                 <  1:448640/149‑1 (MQ=255)
                                                                                                                                    attAACTGCAATTTTAATTGCAGTTCATCACTAAGAATGTGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTc               <  1:968197/147‑1 (MQ=255)
                                                                                                                                      tAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTccc             >  1:553432/1‑149 (MQ=255)
                                                                                                                                      tAACAGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTCTTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTccc             <  2:1238134/149‑1 (MQ=255)
                                                                                                                                      gaaCTGCAATTAAAAATGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGTACAAAAAGCCCAGCGACCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTcac             >  1:124874/2‑147 (MQ=255)
                                                                                                                                           gCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAc        <  1:603391/149‑1 (MQ=255)
                                                                                                                                               ttttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCt    >  2:1377442/1‑149 (MQ=255)
                                                                                                                                                 ttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACgg                                                                                                     <  2:1316812/50‑1 (MQ=255)
                                                                                                                                                 ttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACgg                                                                                                     >  1:1316812/1‑50 (MQ=255)
                                                                                                                                                 ttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCtgt  <  2:808751/149‑1 (MQ=255)
                                                                                                                                                 ttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCtgt  <  1:193931/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGT  >  NC_000913/1996231‑1996524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: