Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 292.9 / ‑6.7 107V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (44/60);  minor base C (2/0);  total (46/61)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 107 total aligned reads displayed.

AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605
                                                                                                                                                 |                                                                                                                                                 
aaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCCGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                <  2:703479/149‑1 (MQ=255)
    ggCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCttt                                                                                                                                            <  2:225852/149‑1 (MQ=255)
    ggCATTCAGTTAGCGCAAAGCAACATCCCTGGCGAAACCTTTAGTTGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATCCGCTTCCGGTTCCCATCTCttt                                                                                                                                            <  2:1481097/149‑1 (MQ=255)
        ttCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACACCAAAGCCAACGCACACCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                                        <  1:555228/149‑1 (MQ=255)
         tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                                                                       <  2:261558/149‑1 (MQ=255)
            ggtAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGGCCGCTGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGAACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    <  1:88322/147‑1 (MQ=255)
            gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    <  2:605850/149‑1 (MQ=255)
              tAGGGCCACGCAACATCCGTCGCGAAAGCTTTCGTGGCCCCCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCCCAGCACACCATGAACCTCTGGCGGGGGAAGACGACGATCCGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                  <  2:1565582/149‑1 (MQ=255)
              tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                  <  2:548434/149‑1 (MQ=255)
              tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                  <  2:1565580/149‑1 (MQ=255)
                tgtcAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACTCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                                                                                <  1:211012/146‑1 (MQ=255)
                 ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATTCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTa                                                                                                                               >  2:12970/1‑149 (MQ=255)
                 ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACCCAATGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCACTTTACAAGGGCGAGTa                                                                                                                               >  2:838615/1‑149 (MQ=255)
                 ggCAAAGCAACAACAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGCCGGGGCAAGACGCCGATACGCTACCGGGTCCCATCTCTTTACAAGGGCGAGTa                                                                                                                               >  2:1421575/1‑149 (MQ=255)
                  gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCACAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                              >  2:630660/1‑149 (MQ=255)
                  gCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCGTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                                              >  2:1517418/1‑149 (MQ=255)
                  gCAAAGCAACATCAGTGGCGAAAGCTTTAGGGGGCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGGGTaa                                                                                                                              <  2:46518/149‑1 (MQ=255)
                   cAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAAc                                                                                                                             >  2:1302058/1‑149 (MQ=255)
                    aaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCATTACAAGGGCGTGTAACa                                                                                                                            >  1:226783/1‑149 (MQ=255)
                     aaGCAACATGAGGGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                           <  2:4022/149‑1 (MQ=255)
                     aaGCAACATCAGTTGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCTGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                           <  2:530993/149‑1 (MQ=255)
                       gCAACATCAGTGGCGAAAGATTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACATCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                         <  1:1415138/149‑1 (MQ=255)
                              cAGTGGCGATAGCTTTAGTGGTCAGCTGCATGCCGCTTCCCAGCTACAGCAAATCCAACGCACATCTAACCATGTACCTCTGGCGGTGGATGACGACGATACGCTTCCGGTTCCCATCTCTTTACTAGGGCGTGTTACAGGCAACAgcg                                                                                                                  <  2:678336/149‑1 (MQ=255)
                              cAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcg                                                                                                                  <  2:683973/149‑1 (MQ=255)
                               aGTGGCTAAAGATTTAGTGGTAAGAAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGAAAACCATCAACCTCTGGCGGGGGAACACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                                                                                                 <  1:1054523/149‑1 (MQ=255)
                                   gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCACCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                                                                             >  2:698894/1‑149 (MQ=255)
                                   gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACACCAAAGCCAACGCACATCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGATTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                                                                             <  1:523595/149‑1 (MQ=255)
                                     gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  1:541646/1‑149 (MQ=255)
                                     gAAAGCTTTAGTGGTCAGCAACAGGCCGCTTCCCAGCAACCGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           <  2:1510735/149‑1 (MQ=255)
                                     gAAAGCCTTAGTGGTCAGCAGCATGCCGCTTCCGAGCAACAGCAATCCCAACGCTCTTCAAACCATGAACCTCTCGCGGGGGAAGACGACGATAAGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACTGGCAACAGCGGCGTTGa                                                                                                           <  1:699900/149‑1 (MQ=255)
                                      aaaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                          <  2:658236/149‑1 (MQ=255)
                                        aGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGatat                                                                                                        <  1:1117371/149‑1 (MQ=255)
                                            ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                    <  1:1364770/149‑1 (MQ=255)
                                            ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                                                                                                    <  1:625176/149‑1 (MQ=255)
                                              aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCCAAGCCAACGCACAGCACACCATGAACCCCAGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  2:1523431/149‑1 (MQ=255)
                                              aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  1:227186/149‑1 (MQ=255)
                                              aGTGGGCAGCAGCAGGCCGCTTCCCAGCAACAGCCAAGCCATCGCACCGCAAACCAGGAACCTCTGGCGGGGGAAGACGACGATACGCATCCGGTTCCCATCTCTTTCCAAGGGCGTGTAACAGGCAACAGCGCCGTTGATAGTTTCGc                                                                                                  <  2:1404344/149‑1 (MQ=255)
                                                tGGTCAGCAGCAGGCCGATTCCCAGCAACAGCAAAGCCAACGGGAAGAAAACCATGATCGTCTGGCGGGGTACTAAGACGATACGCTTCCGGTTCCTATGACTTATCATGGGAGTGTCACGGGCGTATGCGGGGTTTATATTTTAGccg                                                                                                >  2:1161830/1‑148 (MQ=255)
                                                  gTCCGCAGCAGGCCGCGTCCCAGCAACAGCAAAGCCACCGCACAGCAAACCATGAACCTCTGGCGGTGGAAGACGACGATTCCCGTCCGGTTCCCATCTCGTTACAAGGGCGGGTAACAGGCCACCGCGGCGTTGATATTTTCGCCTaa                                                                                              >  1:491497/1‑149 (MQ=255)
                                                  gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                              >  1:91928/1‑149 (MQ=255)
                                                  gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAGATTTTCGCCTaa                                                                                              >  1:211340/1‑149 (MQ=255)
                                                     agcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCACCAGCGGCGTTGATATTTTCGCCTAACGt                                                                                           >  2:453997/1‑149 (MQ=255)
                                                      ccagcagGCCGCTTCCCAGCAACAGCAAAGCCACCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCCTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                                                                          <  1:130880/148‑1 (MQ=255)
                                                         gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                       <  2:48485/149‑1 (MQ=255)
                                                          cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTTCAAGGGCGTGTAACACGCAACAGCGGGGGTGATATTTTCGCCTAACGACagag                                                                                      >  2:86725/1‑149 (MQ=255)
                                                             gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTCACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgggt                                                                                   >  2:1394319/1‑147 (MQ=255)
                                                             gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa                                                                                   >  2:2386/1‑149 (MQ=255)
                                                             gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAGATTATCGCCTAACGTCAGAGGTa                                                                                   >  2:1096809/1‑149 (MQ=255)
                                                              ccGCTTCCCATCAACACCAAAGCCAACGCACAGCAAACCATGAACGTCGGGCGGGGGAAGCCGACGATACGCATCCGGTTCCCATCTCTTTAAACGGACGTGGAACAGGCTACTGCGGGGGTGATAGTTTCGCCTGACGTCAGAGGTAg                                                                                  >  2:1373007/1‑149 (MQ=255)
                                                              ccGCTTCCCAGCAACAGCAAAGCCAACGCGCAGCACACCATGAACCTCTGGCGGGGGTAGACGACGATACGCTTCCGGTTGCCCTCTCTTTACAAGGGCGTGTATCAGGCAACCGCGGCGTGGATATTTTCGCCTGACGTCAGAGGTAg                                                                                  >  2:256152/1‑149 (MQ=255)
                                                                gcgtCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                                                                                <  1:793713/146‑1 (MQ=255)
                                                                  ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTATCAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc                                                                              >  1:1455402/1‑149 (MQ=255)
                                                                    cccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGTCGGGGGAAGACGACGATACGCTTCCGGTTCACATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTTATATTTTCGCCTAACGTCAGAGGTAGCACCGt                                                                            <  2:748725/149‑1 (MQ=255)
                                                                    cccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTACCGTCAGAGGTAGCACCGt                                                                            >  2:1548000/1‑149 (MQ=255)
                                                                     ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGGCTAACGTCAGAGCTAGCACCGTa                                                                           >  2:667200/1‑149 (MQ=255)
                                                                     ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa                                                                           >  2:1227663/1‑149 (MQ=255)
                                                                     ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa                                                                           >  2:553182/1‑149 (MQ=255)
                                                                       agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt                                                                         >  1:619996/1‑149 (MQ=255)
                                                                        tcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                                                                        <  1:5578/148‑1 (MQ=255)
                                                                           acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                     <  1:256737/149‑1 (MQ=255)
                                                                             agcatAGCCTAGGCACAGCAAACCATGAACCTCTGTCGTGGGAAGACGACGTTCCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   <  1:1421575/149‑1 (MQ=255)
                                                                             agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   <  1:1368872/149‑1 (MQ=255)
                                                                               caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCt                                                                 >  2:688824/1‑149 (MQ=255)
                                                                                   gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                             >  1:740927/1‑149 (MQ=255)
                                                                                    ccAACGCACAGCAAACCATCAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCCTCTCTATACAAGGGCGTGTAACAGCCAACACCGGCGTAGATATTTTCGCCTAACGTCACAGGTAGCACCGTAATCCGCGTCTTTAcc                                                            >  1:733687/1‑149 (MQ=255)
                                                                                     cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTAACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTATCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           <  1:962433/149‑1 (MQ=255)
                                                                                         gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt                                                       <  1:1302058/149‑1 (MQ=255)
                                                                                          cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      >  2:1200669/1‑117 (MQ=255)
                                                                                          cacaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      <  1:1200669/117‑1 (MQ=255)
                                                                                           acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCttt                                                     <  2:1163769/149‑1 (MQ=255)
                                                                                            caGCAAACCATGAACCTCTGGCGGGGGGAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTACCCGCTttg                                                    >  1:319717/1‑149 (MQ=255)
                                                                                             aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgt                                                   >  2:73842/1‑149 (MQ=255)
                                                                                                        aaCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGa                                        >  2:1306166/1‑149 (MQ=255)
                                                                                                        aaCCTCTGACGGGGGAAGACGACGATACGCTTCCGTTTCCCATCTCTTAACAAGGGCGTGAAACAGGCAACATCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCTCGTCTTTTCCCCGCTTTTTTGCGCTCAAGa                                        <  2:1377476/149‑1 (MQ=255)
                                                                                                             gtGGAGGTGGAAGACGACTATACCGTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATTTTTTCTCCTATCGTCAGAGGAAGCACCGTAATCCGAGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAg                                   <  2:195373/148‑1 (MQ=255)
                                                                                                             ctGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGGAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAg                                   <  2:1345538/149‑1 (MQ=255)
                                                                                                                  gggggAAGACGACGAAACGCTTCCCGAACCCATCTATTAACAAGGGCGTGTAACAGGCAAAAGCGGCGTTGATATTTTAGCCTAACGTCAGAGGTAGCACCGTAATCAGCGTCTGTTACCCGCTTTGATGCGCTCTAGACGCAAGaaat                              >  1:871584/1‑146 (MQ=255)
                                                                                                                     ggAAGACGAAGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGACTAACGTCAGAGGTAGCTCCGTTATCCGCGTGTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTa                           <  1:86725/149‑1 (MQ=255)
                                                                                                                      gAAGACGACGATCCGCTTCCGGTTCCCATCTCTTTACAAGGGAGTGGAACAGGCAACAGCGGAGTTGATATTTTCGCCTAACGGCAGAGGTAGCACCGTACTCCGCGTCTTTTCCCCGCGTTGTTGCGCTCAAGACGCAGGATAATTAg                          <  1:1025823/149‑1 (MQ=255)
                                                                                                                       aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                         <  1:2386/149‑1 (MQ=255)
                                                                                                                        aGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTGTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGcc                        >  2:1274572/1‑149 (MQ=255)
                                                                                                                         gacgacGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGAAATCCGCGTCTTTTCACCGCTTTGTTGCGCGCAAGACGCAGGAGAATTAGcag                       >  1:703818/1‑147 (MQ=255)
                                                                                                                          acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGa                      <  2:1537904/149‑1 (MQ=255)
                                                                                                                              cgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAg                  <  2:947224/149‑1 (MQ=255)
                                                                                                                              cgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAg                  <  2:1103671/149‑1 (MQ=255)
                                                                                                                               gaTACGCTTCCGGTTCCCATCTCTTGACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCATAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGc                 >  1:95096/1‑149 (MQ=255)
                                                                                                                                aTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACATGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGAGCTCAAGACGCAGGATAATTAGCCGATAAGCa                <  2:226783/149‑1 (MQ=255)
                                                                                                                                 tACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTCACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTGTTCCCCGCTATGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAg               >  2:1502012/1‑149 (MQ=255)
                                                                                                                                   cGCTTGCGGTTCCCTTCTCTTTAGAAGGGCGGGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCAGCGTCTTTTCCCCGCTGTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTa             <  1:1455763/149‑1 (MQ=255)
                                                                                                                                    gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAg            <  2:1341876/149‑1 (MQ=255)
                                                                                                                                     cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc           >  1:744475/1‑149 (MQ=255)
                                                                                                                                     cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc           >  1:95383/1‑149 (MQ=255)
                                                                                                                                     cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc           <  1:1227663/149‑1 (MQ=255)
                                                                                                                                        ccGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac        >  1:899350/1‑149 (MQ=255)
                                                                                                                                         cGGTTCCCATCTCTTTACAAGGGCGGGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGaca       <  2:91928/149‑1 (MQ=255)
                                                                                                                                           gTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTACCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGAcaca     >  1:1334796/1‑149 (MQ=255)
                                                                                                                                            ttCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAg    <  1:1517418/149‑1 (MQ=255)
                                                                                                                                             tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAgg   <  1:630660/149‑1 (MQ=255)
                                                                                                                                             tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAgg   <  1:553182/149‑1 (MQ=255)
                                                                                                                                              cccATCTCTTTACAAGGGCGTGTAACGGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  >  1:821399/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019315‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑