Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,081,688 A→T 9.2% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,6880AT 9.2% 189.2 / 7.2 75I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base A (29/38);  new base T (2/5);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

TTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081540‑2081836
                                                                                                                                                    |                                                                                                                                                    
tttACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCaaaa                                                                                                                                                      >  1:1239224/1‑149 (MQ=255)
  tACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTCCCCACGGTGCAAAAtt                                                                                                                                                    <  2:692959/149‑1 (MQ=255)
     cATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGc                                                                                                                                                 >  1:514006/1‑149 (MQ=255)
      aTCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGATTTCGGTAGCACCATACAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGACATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAttttaaa                                                                                                                                                >  2:543912/1‑146 (MQ=255)
          aGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCATTTTCCCACGGTGCAAAATTTGCACCgt                                                                                                                                            <  1:1362860/149‑1 (MQ=255)
           gggTATATTTGGCTTTGATATGTTGAATTTCGGCTTTCGGAAGCACCATCCAGAACTCTTGATCCGTTCCAAGGTCGATAATTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                           <  1:1395341/149‑2 (MQ=255)
           gggTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                           <  1:1367364/149‑2 (MQ=255)
           gggTATATTTGGCTTTGATATGTGGAATTTCGGCTATCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgta                                                                                                                                           <  2:249476/149‑1 (MQ=255)
           gggTATATTTGGCTTTGATATGTGGAATATCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtg                                                                                                                                           <  1:74147/149‑2 (MQ=255)
             gTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCgtagt                                                                                                                                         >  1:374353/1‑149 (MQ=255)
              tataTTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAATTTTGCACCgtggga                                                                                                                                        <  2:1485855/149‑5 (MQ=255)
                tatTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCCTCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGCTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTaaa                                                                                                                                      <  1:1207676/149‑1 (MQ=255)
                  tttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATTCCAGATGCGTTCCGTTCCTACCTTTTTCCCACGGTGCAAAATTTGCACAGTAGTAAAGt                                                                                                                                    >  1:923641/1‑149 (MQ=255)
                  attGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCAAGAACTCTTGAACAGTTCCCACGTCTATATTTTCTTAATCGCCATACAACATGCGATCCGTTCCGAACTTATTCCAACGGTTCAAAATTTGCACCGTAGAAAAGt                                                                                                                                    >  1:220493/2‑149 (MQ=255)
                   ttGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCTACGGTGCAAATTTTGCACCGTGGGaaaaag                                                                                                                                   <  1:656313/149‑4 (MQ=255)
                   ttGGCTTTGATATGTGGAATTTCGGCTTTCGGTACCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGtt                                                                                                                                   <  1:33213/149‑1 (MQ=255)
                    gggCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTATCCCACGGTGCAAAATTTGCACCGTAGTAAAGttt                                                                                                                                  <  1:1300576/148‑1 (MQ=255)
                       cTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAAATTTGCACCGTAGTAAAGTTttgt                                                                                                                               >  2:794702/1‑149 (MQ=255)
                          tGATATGGGGAATAGCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGGCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTATTGTTGc                                                                                                                            <  2:178918/149‑1 (MQ=255)
                           gATATGTCGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcc                                                                                                                           <  2:409879/149‑1 (MQ=255)
                             tatGTGGAATTTCGGCTTTGGGTAGGACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGcccc                                                                                                                         <  2:1516126/149‑1 (MQ=255)
                               tgtgGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGAGATTTTCTTGATCGCCATACCAGATGCGTTACGTTCCGAGCGTTTTCCCACGGGGCAAAATTTGCACAGTAGTAAAGTTTTTTTGccccga                                                                                                                       >  1:1446539/1‑147 (MQ=255)
                                 tgGAATTTCGGCTTTCGGTAGCCCCCTCCAGAACTCTTGTTCCGTTCCCAGGGCGATCTTTTCTTTATCGCGATCCCCGATGCGTTACGTTCCGCCCTTTTTCCCGCGGTCCATAATTTGCACCTTATGAAAGTTTTGTTGACACCAAc                                                                                                                     >  2:962795/1‑149 (MQ=255)
                                    aaTTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCa                                                                                                                  <  2:1147545/149‑1 (MQ=255)
                                        tCGGCTTTCGGTAGCACGATCCATAACTCTTGTTCCGTTCACAGGTTGATATTTTCTTTATCGCCTTACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGTTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTaa                                                                                                              <  2:1349600/149‑1 (MQ=255)
                                         cGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGCTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCAACGTAGTAAAGTTTTGTAGACCCCAACGCAGTAAt                                                                                                             >  1:71636/1‑149 (MQ=255)
                                          ggCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAtt                                                                                                            >  2:1487173/1‑149 (MQ=255)
                                           gCTTACGGTAGCACCATACAGAACTCTTGTTACGTTCCCAGGTCGATATTTTCTTTATCTCCATACCAGATGCGTTACGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACAGTAGTAAAGTTTTGTAGCCCCCTACGCAGTAAttt                                                                                                           >  2:135977/1‑149 (MQ=255)
                                             tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGCCCTTTTTCCCACGGTGCAAAATTTGCACCCTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTc                                                                                                         >  1:23333/1‑149 (MQ=255)
                                             tttCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTc                                                                                                         >  1:17283/1‑149 (MQ=255)
                                               tCGGTAGCACCATCCAGAACTCATGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                                                                                                       <  2:1537026/149‑1 (MQ=255)
                                                  gTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGACGATATTTTCTTAATCCCCATACCAGCTGCGTTCCGTTACGACCATATTCCCACCCTGCAAACTTTTAACCGTAGTAAAAAATAGTTTCCCCCAACGCAGTAAATTTATAGcc                                                                                                    >  2:408391/1‑149 (MQ=255)
                                                       aCCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTc                                                                                               <  2:560725/149‑1 (MQ=255)
                                                          aTCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGACCCCAACGCAGTAATTTTCTTGCCTCTTCCTc                                                                                            >  1:1223191/1‑149 (MQ=255)
                                                            ccAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCAc                                                                                          <  1:795553/149‑1 (MQ=255)
                                                              aGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGa                                                                                        <  2:621345/149‑1 (MQ=255)
                                                                aaCTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCGATACCAGATGCGTTCCGGTCGGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAcc                                                                                      <  2:1052785/149‑1 (MQ=255)
                                                                    ctTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt                                                                                  >  2:1360161/1‑149 (MQ=255)
                                                                     ttGATCCGGTCCCAGCTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg                                                                                 <  2:1016651/149‑1 (MQ=255)
                                                                      tGTTCCGTTCCCAGGGCGATATTTTCTTTATCGCCATACCCGATGCGTTCCGTTCCGACCTTTTTCCCACGGGGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCCGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGc                                                                                <  2:1498041/149‑1 (MQ=255)
                                                                            ggtccCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTc                                                                          <  2:1385081/147‑1 (MQ=255)
                                                                                ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                      >  2:230329/1‑149 (MQ=255)
                                                                                ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                      >  2:393966/1‑149 (MQ=255)
                                                                                ccAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGcc                                                                      <  1:1313834/149‑1 (MQ=255)
                                                                                 cAGGTCGATATTTTCTTTATCGCCATACCATATGCGTTCCGTTCCGACATTTTTCCCACGGTGAAAAATTTGAAAAGTAGTTAAGTTTTGTTGACCCCAAAGCAGTAATTTTCTTGCCTCTTCATCACGACCTTTTGAACTGTCatcgc                                                                     >  2:1211522/1‑145 (MQ=255)
                                                                                   ggTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGccccc                                                                   <  2:374353/149‑1 (MQ=255)
                                                                                        atatTTTCTTTATCGCCATACCATATGCGTTCCGTACCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAATTTTTGTAGCCCCCAACGCAGTAATTTTCATGCCTCTTCCTCACGACCTTTTGCACTGGCAGCCCCCATTAc                                                              <  1:23745/149‑1 (MQ=255)
                                                                                          atTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCCACGCAGTAATTTTCTTGCCTCTTCCTGACGACCTTTTGCACTGTCAGCCCCCATTACCa                                                            >  2:1140731/1‑149 (MQ=255)
                                                                                            tttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACt                                                          <  2:1475802/149‑1 (MQ=255)
                                                                                             ttCTTTATCGCCATACCAGATGCGGTCCGTTCCGACCTTTTGCCAACTGTGCAAAATTTTCAACGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTg                                                         <  1:377526/149‑1 (MQ=255)
                                                                                              tCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCACCCAACGCAGTAATTTTCTTGCCTCTTCCTCAAGACCTTATGCACTGTCAGCCCACATTACCACtta                                                        >  1:311050/1‑147 (MQ=255)
                                                                                                 ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAAt                                                     <  1:146669/149‑1 (MQ=255)
                                                                                                 ttATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAAt                                                     <  1:1487173/149‑1 (MQ=255)
                                                                                                 ttATCGCCATACCAGATGCGTTCCGTTCCGACCTATTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTATTGGTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAAt                                                     <  2:1242031/149‑1 (MQ=255)
                                                                                                  tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACTGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATg                                                    <  1:794702/149‑1 (MQ=255)
                                                                                                   aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTCATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCCCTGCAATga                                                   >  2:988741/1‑149 (MQ=255)
                                                                                                      gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgac                                                <  2:681325/149‑1 (MQ=255)
                                                                                                       ccATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAgacg                                               >  1:985371/1‑149 (MQ=255)
                                                                                                             cAGAAGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTg                                         <  1:1116890/149‑1 (MQ=255)
                                                                                                               gATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGcc                                       >  2:130760/1‑149 (MQ=255)
                                                                                                                      tccgttccgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTAc                                <  2:985371/149‑1 (MQ=255)
                                                                                                                       ccgttccgACCTTTTTCCCACTGTCCAAAATTTGCACCGTAGTAAAGTTTTGTTGCACCAAACGCACTAATTTTATTTCCTCTTCCTCAAGAACTTTTTCAATGTAAGCCCCCATTACCACTGCAATCAGACGACGCTGCGCAACTACa                               >  2:708205/1‑149 (MQ=255)
                                                                                                                             cgACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGaa                         <  1:1370022/149‑1 (MQ=255)
                                                                                                                              gACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTAAGCCCCCATTACCACTGCAATGAGACGACGCTGCGCATCTACAGCCGAAg                        >  2:143834/1‑149 (MQ=255)
                                                                                                                                 cTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGATACGACGCTGCCCATCTACAGCCGAAGCaa                     >  1:1164132/1‑149 (MQ=255)
                                                                                                                                 cTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCaa                     <  1:1242495/149‑1 (MQ=255)
                                                                                                                                 cTTTTTCCCAAGTTTCAAAATTTTAACATTAGTAAAGTTTTGTTGCCCCCAACGAAGTAATTTTCTTTACTCTTCCTCAAGACCTTTTGCTCTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCaa                     <  1:1112786/149‑1 (MQ=255)
                                                                                                                                  tttttCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAAt                    <  1:696901/149‑1 (MQ=255)
                                                                                                                                   ttttCCCACCGTGCAAAATTTGAACCGTAGTAAAGTTTTGTTGCCACCAACGCAGTAATTTTCTTGCCTCTTCCTCACTACCTTTTGCACTGTCAGCGCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATg                   <  1:408391/149‑1 (MQ=255)
                                                                                                                                    tttactaCGGTGCAAATTTTGCACCGTGGGAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATga                  >  1:18750/7‑149 (MQ=255)
                                                                                                                                         taCGGTGCAAATTTTGCACCGTGGTAAAGTTTTGTTGCCCCCAACGCACTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTCCCACTGCACTGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGAttt             >  1:398885/2‑148 (MQ=255)
                                                                                                                                         cACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAGCGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGACGCAATGAGATTa             >  1:24988/1‑149 (MQ=255)
                                                                                                                                         cACGGTGCAAAATTTGCACCGTAGTAAAGTTGTGTTGCCCCCCGCGCAGTAATTTTCTTGACTCTTCCTCCCGACCTTTTGGACTGTCAGCCCCCATTACCACTGCAATGAGCCGACGCTGACCATCTACAGCCGAAGCAATGAGAtgt             >  1:482612/1‑147 (MQ=255)
                                                                                                                                         aacgtttcAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACTCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTa             <  1:543912/142‑1 (MQ=255)
                                                                                                                                          aCGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTaa            >  1:292118/1‑149 (MQ=255)
                                                                                                                                          aCGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTaa            >  1:340593/1‑149 (MQ=255)
                                                                                                                                              tGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAAccc        <  2:514006/149‑1 (MQ=255)
                                                                                                                                                 aaaaTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCTGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCTAAGCAATGAGAATAAACCCGGc     >  2:622421/1‑149 (MQ=255)
                                                                                                                                                  aaaTTTGCACCCTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCGACTTCCTCCCGACCTTTTGCACTGGCCGCCCCCATTACCTCTGCAATGAGACGACGCTGCCCATATACAGCCCAAGCAATGAGATTAAACCCGGcc    >  2:828020/1‑148 (MQ=255)
                                                                                                                                                    aTTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCAcc  >  1:47119/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTTACCATCAAGGGTATATTTGGCTTTGATATGTGGAATTTCGGCTTTCGGTAGCACCATCCAGAACTCTTGTTCCGTTCCCAGGTCGATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACC  >  NC_000913/2081540‑2081836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑