Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,126,158 A→T 5.8% F22Y (TTT→TAT)  wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,126,1580AT 5.8% 281.1 / 3.2 106F22Y (TTT→TAT) wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base A (51/48);  new base T (5/2);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 107 total aligned reads displayed.

CCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGAT  >  NC_000913/2126010‑2126304
                                                                                                                                                    |                                                                                                                                                  
ccccAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATaa                                                                                                                                                    >  1:203548/1‑149 (MQ=255)
    aGTTCCGCCATCGTCAGCCGCTCAAATGCTGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTc                                                                                                                                                <  1:1195482/149‑1 (MQ=255)
     gTTCCGCCATCGTCAGCCGCTCACATGCGGCTTCCAGCGTCTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCg                                                                                                                                               <  1:650511/149‑1 (MQ=255)
         cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaa                                                                                                                                           <  2:1132794/149‑2 (MQ=255)
         cGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTTTATTGTCgagaa                                                                                                                                           <  1:283674/149‑2 (MQ=255)
          gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCgagaga                                                                                                                                          <  2:723475/149‑1 (MQ=255)
          gCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCcagagt                                                                                                                                          >  2:775320/1‑148 (MQ=255)
            cATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTGCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGCTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTAATCGACAATAAAGTCgagagaga                                                                                                                                        >  1:1142872/1‑149 (MQ=255)
                 tCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCCCGCGCCCACCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGc                                                                                                                                   <  2:427400/149‑1 (MQ=255)
                 gcAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGc                                                                                                                                   <  2:602709/148‑1 (MQ=255)
                     ccGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAGCTCGCCGCGACTGTTCTCGACAATATAGTCGAGAGAGAGAAGCGGAg                                                                                                                               >  1:607625/1‑149 (MQ=255)
                     ccGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAg                                                                                                                               >  1:794655/1‑149 (MQ=255)
                      cGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAGCTCGCCGCGACTGTTCTCGACATTAAAGTCGAGAGAGACATGCGGAGt                                                                                                                              >  1:109211/1‑149 (MQ=255)
                      cGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGt                                                                                                                              <  2:1065430/149‑1 (MQ=255)
                       gCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTg                                                                                                                             <  1:969921/149‑1 (MQ=255)
                       gCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTg                                                                                                                             <  2:1005806/149‑1 (MQ=255)
                              tGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCa                                                                                                                      <  1:142678/149‑1 (MQ=255)
                                 ggCTTCCAGCGTTTCGTCTGTCTGCACGCGCCCTCCCGGGACACGCCAGTAACCGTGCGCCGGGCGGGTGGTTCTGTTGCCAAGCAGAACAGAGACGAGACTGTTGTCGACAATCTAGGCGAGTGAGACCAGCGGAGGGGCGCGGAcgc                                                                                                                   >  2:668684/1‑147 (MQ=255)
                                   cTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTAcc                                                                                                                 <  1:1252170/149‑1 (MQ=255)
                                     tCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGt                                                                                                               <  1:86012/149‑1 (MQ=255)
                                     tCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTGGGATCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGt                                                                                                               <  1:479277/149‑1 (MQ=255)
                                      ccAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTg                                                                                                              >  2:1158506/1‑149 (MQ=255)
                                       cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGCAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                             >  2:47582/1‑149 (MQ=255)
                                       cAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTgg                                                                                                             >  2:806419/1‑149 (MQ=255)
                                           gTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGGTGGCTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAACTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCaaa                                                                                                         <  2:91556/149‑1 (MQ=255)
                                              tCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTc                                                                                                      >  1:1281996/1‑149 (MQ=255)
                                              tCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTc                                                                                                      <  1:1074246/149‑1 (MQ=255)
                                                gTCTTTCTGCACGCGCCCTCCCGGCACAACCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTACCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGCGTGGAGCGCACTACCGGGTCAAAGACtt                                                                                                    >  2:1057732/1‑149 (MQ=255)
                                                gTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACAACCGTGGCAAAGTCtt                                                                                                    >  2:1296247/1‑149 (MQ=255)
                                                 tCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCTGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACATTAAAGTCGAGAGAGACAAGCGGAGTGGAGCTCACTACCGTGGACAAGTCTTc                                                                                                   >  1:1148066/1‑149 (MQ=255)
                                                 tCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACTATAACGTCGAGAGAGCCAAGCGGAGTGGCGCGCACTACCGTGGCAAAGGCTTc                                                                                                   >  1:311347/1‑149 (MQ=255)
                                                 tCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTc                                                                                                   >  2:5658/1‑149 (MQ=255)
                                                 gcTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTc                                                                                                   <  2:875328/148‑1 (MQ=255)
                                                  cTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTcc                                                                                                  <  1:281643/149‑1 (MQ=255)
                                                   tttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                                 <  1:1532116/149‑1 (MQ=255)
                                                   tttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCt                                                                                                 <  1:784404/149‑1 (MQ=255)
                                                    ttCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGAGGAGCGCACTACCGTGGCAAAGTCTTCCTg                                                                                                >  2:657745/1‑149 (MQ=255)
                                                        gCACGCGCCCTCCCGGCACAAACCAGTACCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGTCAAAGTCTTCCTGACGt                                                                                            >  2:195065/1‑149 (MQ=255)
                                                         cACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCAAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTa                                                                                           >  2:542682/1‑149 (MQ=255)
                                                             cgcCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTaaaaa                                                                                       <  1:749293/149‑1 (MQ=255)
                                                                ccTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcat                                                                                    >  2:1378046/1‑149 (MQ=255)
                                                                 cTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatc                                                                                   >  2:757879/1‑149 (MQ=255)
                                                                  tCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatca                                                                                  <  2:216382/149‑1 (MQ=255)
                                                                  tCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAcatca                                                                                  >  1:1430293/1‑149 (MQ=255)
                                                                    ccGGCCCAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCACGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                >  1:1466557/1‑149 (MQ=255)
                                                                    ccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                <  1:5658/149‑1 (MQ=255)
                                                                    ccGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCGTTTGCCAAGCTGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGCGAGAGACAAGCGGCGCGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCAtt                                                                                >  1:631567/1‑149 (MQ=255)
                                                                     cGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTAACGTAAAAACATCATTa                                                                               >  2:1018265/1‑149 (MQ=255)
                                                                          caAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTAccccc                                                                          <  2:1249279/149‑1 (MQ=255)
                                                                           aaaCCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAACACCATCCTTACACCCg                                                                         >  2:508022/1‑149 (MQ=255)
                                                                           aaaCCAGTAACCCTGCGCCGGGCGGTTCGTTCATTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCCACAAAAAAGTCGAGACAGACAAGCGGAGTGGAGCGCACTACAGTCGCAAAGTCTTCCTGAAGAAAAAACATCATAAccccag                                                                         >  2:1356884/1‑147 (MQ=255)
                                                                                 gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                   <  1:762703/149‑1 (MQ=255)
                                                                                 gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCg                                                                   <  1:542682/149‑1 (MQ=255)
                                                                                 gTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGAGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACCTCATTACCCCCGAAAGCg                                                                   >  1:314269/1‑149 (MQ=255)
                                                                                   aaCCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGGAGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTCGCAAAGTCTTCCTGACGTAAAAACATGATTACCACCGAAAGCGGt                                                                 >  1:81621/1‑149 (MQ=255)
                                                                                         gcgcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGAt                                                           <  1:227108/149‑1 (MQ=255)
                                                                                          cgcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATTATTACCCCCGAAAGCGGTCTTGAtt                                                          <  1:888838/149‑1 (MQ=255)
                                                                                           gcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAACGTCGAGAGAGCCAAGCGGAGTGGAGCGCACAACCGTGGCCAAGTCGTACTGACGTAAACACATCATTACCCCCGATCGCGGTCtgtgttc                                                         >  1:1500125/1‑143 (MQ=255)
                                                                                           gcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtc                                                         >  1:1363044/1‑149 (MQ=255)
                                                                                           gcCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtc                                                         <  2:1245349/149‑1 (MQ=255)
                                                                                            ccGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtct                                                        <  1:1378046/149‑1 (MQ=255)
                                                                                             cgggcgggTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTGCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATtctc                                                       <  2:1358993/149‑1 (MQ=255)
                                                                                              gggCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAGACATCATTACCCCCGAAAGCGGTCTTGATTCTCa                                                      <  1:423990/149‑1 (MQ=255)
                                                                                               ggaggtgggttCTTTTGCCAAGCAGAAACTCGCCGAGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCaa                                                     <  1:904143/146‑1 (MQ=255)
                                                                                               ggCGGTTGGTTCTTTTGCCAAGAAGAAACTCGCCGCGACTTTTCTCGACAATAAAGTAGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCaa                                                     <  1:908873/149‑1 (MQ=255)
                                                                                                    ttggttCTTTGGCCACGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAAc                                                <  2:607625/149‑1 (MQ=255)
                                                                                                      ggttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCa                                              >  1:555190/1‑149 (MQ=255)
                                                                                                       gttCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAATACATCATTACCCCCGAAAGCTGTCTTGATTCTCAAGGAAGCAc                                             >  1:849699/1‑149 (MQ=255)
                                                                                                          cTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCTTTGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTgg                                          <  2:1142872/149‑1 (MQ=255)
                                                                                                          cTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTgg                                          <  1:958629/149‑1 (MQ=255)
                                                                                                           ttttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGt                                         >  1:1537029/1‑149 (MQ=255)
                                                                                                             ttGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGCAGCGCACTACCGTGGCAAAGTATTCCTGACGTAAAAACATCATTACCCCCGAAAGCTGACTTGATTCTCAAGGAACCACTGGTaa                                       >  2:1520675/1‑149 (MQ=255)
                                                                                                              tGCCAAGCAGAAACTCGCCGCGAATGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAg                                      <  1:1018265/149‑1 (MQ=255)
                                                                                                               gCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGCCAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGt                                     >  2:294131/1‑149 (MQ=255)
                                                                                                                 cAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCACAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGc                                   >  1:942832/1‑149 (MQ=255)
                                                                                                                  aaGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCt                                  <  1:1158506/149‑1 (MQ=255)
                                                                                                                    tcAGAAACTCTCCCCTACTTTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGAACTAACGTGGCAAAGTCTTCCTGACGTAAATACATCATTACGCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTgg                                <  2:38417/148‑1 (MQ=255)
                                                                                                                     cAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGc                               <  2:256124/149‑1 (MQ=255)
                                                                                                                       gAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGCGTGGAGCGCACTCCCGTGGCAAAGTCTTCCTGACGTAAACACATCCTTACCCCCGCTAGCGGGCTGGATTCTCCAGGTCGCACTGGTAAGTGCTGGCac                             >  2:1026869/1‑148 (MQ=255)
                                                                                                                        aaaCTCGCCGCGACTGTTCTCGACAATATAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAAGATCATTTCCCCCGAAAGCGGTCTTGATTCTCAAGGAACCAGTGGTAAGTGCTGGCAAg                            >  1:60125/1‑149 (MQ=255)
                                                                                                                        aaaCTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAg                            <  2:583346/149‑1 (MQ=255)
                                                                                                                        aaaCTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTAGATTCTCACGGAACCCCTGGTAAGTGCTGGaaac                            >  2:747936/1‑148 (MQ=255)
                                                                                                                         aaCTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGc                           <  2:203548/149‑1 (MQ=255)
                                                                                                                         aaCTCGCCGCGACGGTTATAGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGTCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGc                           <  2:1148066/149‑1 (MQ=255)
                                                                                                                                  cgACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTcc                  >  1:1124728/1‑149 (MQ=255)
                                                                                                                                     cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt               <  1:1146555/149‑1 (MQ=255)
                                                                                                                                     cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGt               >  2:1166490/1‑149 (MQ=255)
                                                                                                                                     cTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGATTCCAGt               >  2:1160730/1‑149 (MQ=255)
                                                                                                                                      tGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTg              >  1:1350202/1‑149 (MQ=255)
                                                                                                                                       gTTCGCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGGCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTga             <  1:1448409/149‑1 (MQ=255)
                                                                                                                                        ttCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag            >  1:665566/1‑149 (MQ=255)
                                                                                                                                        ctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTTGAGCGCACTACCGTGTCAAAGTCTTCCTCACGTAAAAACATCATTACCCACGAAATCGGTCTTGATTCTAAAGGAACCACTGGTAAGTGCTGGCAAGCCACTCTTCCAGTgag            >  2:887994/2‑149 (MQ=255)
                                                                                                                                        ctctcGACTTTATTGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGATAGCTGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTgag            >  1:1030122/2‑149 (MQ=255)
                                                                                                                                           tcGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAGGCGGTCTTGATTCTCAAGGACCCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGAtt         >  2:569618/1‑149 (MQ=255)
                                                                                                                                           tcGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGAtt         >  1:306558/1‑149 (MQ=255)
                                                                                                                                              aCAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCg      <  2:788557/149‑1 (MQ=255)
                                                                                                                                              aCAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCg      <  1:806419/149‑1 (MQ=255)
                                                                                                                                              aCAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCg      <  1:47582/149‑1 (MQ=255)
                                                                                                                                                  tAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGAt  >  2:80208/1‑149 (MQ=255)
                                                                                                                                                  tAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGAt  >  1:906166/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCCCAGTTCCGCCATCGTCAGCCGCTCAAATGCGGCTTCCAGCGTTTCGTCTTTCTGCACGCGCCCTCCCGGCACAAACCAGTAACCCTGCGCCGGGCGGTTGGTTCTTTTGCCAAGCAGAAACTCGCCGCGACTGTTCTCGACAATAAAGTCGAGAGAGACAAGCGGAGTGGAGCGCACTACCGTGGCAAAGTCTTCCTGACGTAAAAACATCATTACCCCCGAAAGCGGTCTTGATTCTCAAGGAACCACTGGTAAGTGCTGGCAAGCCCCGCTTCCAGTGAGATTTCGTGAT  >  NC_000913/2126010‑2126304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑