BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,165,264	A→G	5.5%	T26A (ACT→GCT) ‡	yegP →	UPF0339 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,264	0	A	G	5.5%	310.0 / 6.4	109	T26A (ACT→GCT) ‡	yegP	UPF0339 family protein
Reads supporting (aligned to +/- strand): ref base A (56/47); new base G (3/3); total (59/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 111 total aligned reads displayed.

TCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATC  >  NC_000913/2165119‑2165411

tCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTa                                                                                                                                                  >  1:896414/1‑149 (MQ=255)

  aTACTTTATTCTGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCATTCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                                                                                                                                                <  2:1072798/149‑1 (MQ=255)

  aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                                                                                                                                                >  2:1244918/1‑149 (MQ=255)

   tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATcc                                                                                                                                               >  1:782120/1‑149 (MQ=255)

      tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                                                                                                                                            >  2:919608/1‑149 (MQ=255)

       ttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCCATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTccc                                                                                                                                           <  2:1483108/149‑3 (MQ=255)

       ttAATAGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGGGAGACTATCCTCAc                                                                                                                                           <  1:104612/149‑1 (MQ=255)

           tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGATCTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGc                                                                                                                                       >  1:1082499/1‑149 (MQ=255)

           tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCatt                                                                                                                                       <  2:775777/149‑3 (MQ=255)

           tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCatt                                                                                                                                       <  2:58112/149‑3 (MQ=255)

             ggTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGGGAAAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCattgc                                                                                                                                     <  2:654936/149‑5 (MQ=255)

              gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGCTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAg                                                                                                                                    >  1:81026/1‑149 (MQ=255)

                 aaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCtt                                                                                                                                 <  2:267292/149‑1 (MQ=255)

                 aaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCtt                                                                                                                                 <  1:1552694/149‑1 (MQ=255)

                   cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta                                                                                                                               <  1:101422/149‑1 (MQ=255)

                   cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta                                                                                                                               >  1:1347304/1‑149 (MQ=255)

                    aGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGTAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGAAGTGATAATCAGTTCCGGTTTGTGCTAACAGCGGGCAATGGTGAGACTATCCTCACCAGATAGCTTtat                                                                                                                              >  2:177999/1‑149 (MQ=255)

                        ccTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCt                                                                                                                          <  1:1357693/149‑1 (MQ=255)

                         cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTc                                                                                                                         >  2:766651/1‑149 (MQ=255)

                         cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTc                                                                                                                         >  2:820790/1‑149 (MQ=255)

                            tAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCaaa                                                                                                                      <  2:124777/149‑1 (MQ=255)

                                   tGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGTTTTGAACTCAGTAAGAGCAGTGATAATAAGTTCCGGTTTGTGCTAAAATCGGGCAATTGTGAGACTATCCTAACCAGAGAGCTTTATACCTCAAAAACctct                                                                                                               <  2:686651/149‑1 (MQ=255)

                                    tttttCGTTACACAATTTCAATCAAGTAGTCATTATGGCTGTTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                              <  2:127455/148‑1 (MQ=255)

                                    gTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                              >  1:1375678/1‑149 (MQ=255)

                                    gTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                              <  1:776819/149‑1 (MQ=255)

                                     ttttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGc                                                                                                             >  2:408746/1‑149 (MQ=255)

                                      tttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACCCTCTGCg                                                                                                            <  2:782120/149‑1 (MQ=255)

                                      tttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCg                                                                                                            <  2:176235/149‑1 (MQ=255)

                                            tACACAATTTCAATCAAGGAGTCATTATGGCTGGGTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAg                                                                                                      <  2:937315/149‑1 (MQ=255)

                                              cacaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAggg                                                                                                    >  2:522120/1‑149 (MQ=255)

                                               acaATTTCAATCAAGGAGTCATTTTGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACAAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGc                                                                                                   >  1:1094263/1‑149 (MQ=255)

                                               acaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAggtc                                                                                                   >  1:662607/1‑147 (MQ=255)

                                               acaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGc                                                                                                   >  2:587892/1‑149 (MQ=255)

                                                 aaTTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCAt                                                                                                 >  2:479696/1‑149 (MQ=255)

                                                   tttCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTGTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATcg                                                                                               <  1:1099751/149‑1 (MQ=255)

                                                      caatcaaGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGt                                                                                            <  1:778633/149‑1 (MQ=255)

                                                       aatcaaGTAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTGTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCTGAAAAGGGCATCGCGTc                                                                                           <  2:155303/149‑1 (MQ=255)

                                                       aatcaaGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTACACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTc                                                                                           >  2:1469777/1‑149 (MQ=255)

                                                       aatcaaGGAGTCATTATGGCTGGCTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGGGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTc                                                                                           <  2:452438/149‑1 (MQ=255)

                                                        atcaaGGAGTCATTATGGCTGGTTGGATTGAACTCAGTAAGATCAGTGAAAATCAGTTACGGTTTGTGCTAAAAGAGAGCAATTGTTAGACTATCCTCACCTTCTAGCTTTATAAATCAAAAACCTCTGCGGAAAAGCGCATAGCGtag                                                                                          >  1:311313/1‑147 (MQ=255)

                                                         tcaaGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCgg                                                                                         >  2:1209495/1‑149 (MQ=255)

                                                         tcaaCGAGTCATTATGTATTGTTGCTTTTATCGCATTAAGAGCAGTTATAAACACTTCCGGTTTTTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCgg                                                                                         <  1:1061737/149‑1 (MQ=255)

                                                            aGGAGTCATTATGGCTGGTTGGGTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACGCTCAGCGGAAAAGGGCCTCGCGTCGGTGc                                                                                      >  2:258866/1‑149 (MQ=255)

                                                                gTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAg                                                                                  >  1:1424524/1‑149 (MQ=255)

                                                                 tCATTATGGCTGGGTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACCCTCTGCGGAAAAGGGCATCGCGGCGGTGCGTAGc                                                                                 <  2:476733/149‑1 (MQ=255)

                                                                   aTTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCaa                                                                               >  1:373182/1‑149 (MQ=255)

                                                                    ttATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAAc                                                                              >  2:542926/1‑149 (MQ=255)

                                                                     tATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACa                                                                             <  1:426272/149‑1 (MQ=255)

                                                                       tggctggcTGGTTTGAACTAAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGc                                                                           <  1:273548/149‑1 (MQ=255)

                                                                         gCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGccc                                                                         <  1:1128753/149‑1 (MQ=255)

                                                                                ggttTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGCGCTTTATACCTCAAAAACCTCAGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCaagaa                                                                  >  2:270919/1‑149 (MQ=255)

                                                                                 gttTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCaagaag                                                                 >  2:1475758/1‑149 (MQ=255)

                                                                                 gttTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATACTCCCCAGCTAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCACCAGCCCGCaagaag                                                                 >  2:1155689/1‑149 (MQ=255)

                                                                                   ttGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAgaagaa                                                               <  1:766651/149‑1 (MQ=255)

                                                                                    tGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAAc                                                              <  1:1511557/149‑1 (MQ=255)

                                                                                     gAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACg                                                             <  2:457501/149‑1 (MQ=255)

                                                                                       aCTGAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATTTTGAGATTATACTCACCAGCGTGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCAGCGCGGCGGGGCGGAGCAACAGCCCGCAAGAAGCACGCt                                                           <  1:842626/149‑1 (MQ=255)

                                                                                         tCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTAt                                                         >  1:1264006/1‑149 (MQ=255)

                                                                                            gTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGAGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATgag                                                      >  2:609334/1‑149 (MQ=255)

                                                                                                gagCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGaaaa                                                  >  2:558047/1‑149 (MQ=255)

                                                                                                gagCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGaaaa                                                  <  2:790328/149‑1 (MQ=255)

                                                                                                  gCAGTGAGAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAATGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTAGGAGaaaaaa                                                >  2:1322617/1‑149 (MQ=255)

                                                                                                    aGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAAc                                              <  2:648053/149‑1 (MQ=255)

                                                                                                    aGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAAc                                              <  2:163329/149‑1 (MQ=255)

                                                                                                     gTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGGAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACg                                             <  2:216210/149‑1 (MQ=255)

                                                                                                      tGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACgg                                            <  1:524141/149‑1 (MQ=255)

                                                                                                        aTAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCa                                          >  1:755315/1‑149 (MQ=255)

                                                                                                        aTAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTCCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCa                                          >  1:711613/1‑149 (MQ=255)

                                                                                                         tAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCaa                                         >  1:1130280/1‑149 (MQ=255)

                                                                                                         tAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCaa                                         <  1:205193/149‑1 (MQ=255)

                                                                                                          aaTCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAg                                        <  1:565266/149‑1 (MQ=255)

                                                                                                           aTCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCTCGTCGGTGCGTAGCAACAGCCCGCAATAAGAACGCTATGAGAAAAAAACGGCAAGt                                       <  2:1424524/149‑1 (MQ=255)

                                                                                                           aTCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGt                                       <  1:1244918/149‑1 (MQ=255)

                                                                                                            tCAGTTGCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTa                                      >  1:702714/1‑149 (MQ=255)

                                                                                                               gTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAATAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGAAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACg                                   <  2:1568256/149‑1 (MQ=255)

                                                                                                                    ggTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCCTCTCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCaaa                              >  1:1243546/1‑149 (MQ=255)

                                                                                                                    ggTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCaaa                              <  1:777314/149‑1 (MQ=255)

                                                                                                                      tttGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAAtt                            >  1:1340922/1‑149 (MQ=255)

                                                                                                                        tgtgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGGCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCt                          <  1:973455/149‑1 (MQ=255)

                                                                                                                          tgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTAt                        >  2:1543465/1‑149 (MQ=255)

                                                                                                                           gCTAAAAGCGGGCAATGGTGAGACTATCCTCCCCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTAtt                       >  1:981323/1‑149 (MQ=255)

                                                                                                                           gCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTAtt                       >  1:1164134/1‑149 (MQ=255)

                                                                                                                            cTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTAATACCTCAAAACCCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGATAAAAAAGGCAAGTAACGGCAAATGCTAttt                      >  1:1521046/1‑149 (MQ=255)

                                                                                                                             tAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTc                     >  1:1557528/1‑149 (MQ=255)

                                                                                                                               aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCaa                   >  1:557296/1‑149 (MQ=255)

                                                                                                                               aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCaa                   >  1:631216/1‑149 (MQ=255)

                                                                                                                               aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCaa                   >  1:1123288/1‑149 (MQ=255)

                                                                                                                               aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAAAGGCAAATTCTATTTCaa                   >  2:1387847/1‑149 (MQ=255)

                                                                                                                                 aGCGGGCAATGGTGAGACTTTCCTCACCAGCGAGCGTTATACCTCAAAAACCTCAGCGGAACAGGGCATCGCGGCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGATAAAAAAACGGCAAGTAAAGGCAAATTCTATTTCAATc                 <  2:1250076/149‑1 (MQ=255)

                                                                                                                                  gCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCt                <  2:81026/149‑1 (MQ=255)

                                                                                                                                     ggCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACCGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTTTTTCAATCTGaa             >  2:154767/1‑149 (MQ=255)

                                                                                                                                      tcgctGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGaaa            >  1:1265655/5‑149 (MQ=255)

                                                                                                                                      gccaTGGTGAGACTATCCTCACCAGCTAGCTTTATACCTCAAAAAACTCTGCGGACACGGGCATCGCGTCGGTTCTTAGCAACAGCCCGCAAGAAGAACGCTATTATAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGaaa            <  2:1062357/146‑1 (MQ=255)

                                                                                                                                        gctGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGc          >  1:30282/3‑149 (MQ=255)

                                                                                                                                        aaTGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGc          >  2:1536059/1‑149 (MQ=255)

                                                                                                                                          tGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAACCGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCg        >  2:78251/1‑149 (MQ=255)

                                                                                                                                           ggggagaCTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGc       <  2:1438551/146‑1 (MQ=255)

                                                                                                                                             tGAGACTATCCTCACCAGCTAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTa     >  1:232090/1‑149 (MQ=255)

                                                                                                                                             tGAGACTATCCTCACCAGCGAGCTTTATACCTCAATAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACCGCCCGCAAGAAGCACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTa     >  2:202987/1‑149 (MQ=255)

                                                                                                                                             tGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAACCGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTa     >  2:1372298/1‑149 (MQ=255)

TCATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATC  >  NC_000913/2165119‑2165411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`