BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,165,269	C→T	5.6%	I27I (ATC→ATT)	yegP →	UPF0339 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,269	0	C	T	5.6%	309.6 / 6.4	108	I27I (ATC→ATT)	yegP	UPF0339 family protein
Reads supporting (aligned to +/- strand): ref base C (52/48); new base T (3/3); total (56/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 112 total aligned reads displayed.

ATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATC  >  NC_000913/2165121‑2165414

aTACTTTATTCTGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCATTCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                                                                                                                                                   <  2:1072798/149‑1 (MQ=255)

aTACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATc                                                                                                                                                   >  2:1244918/1‑149 (MQ=255)

 tACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATcc                                                                                                                                                  >  1:782120/1‑149 (MQ=255)

    tttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCa                                                                                                                                               >  2:919608/1‑149 (MQ=255)

     ttAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCCATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTccc                                                                                                                                              <  2:1483108/149‑3 (MQ=255)

     ttAATAGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGGGAGACTATCCTCAc                                                                                                                                              <  1:104612/149‑1 (MQ=255)

         tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGATCTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGc                                                                                                                                          >  1:1082499/1‑149 (MQ=255)

         tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCatt                                                                                                                                          <  2:775777/149‑3 (MQ=255)

         tCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCatt                                                                                                                                          <  2:58112/149‑3 (MQ=255)

           ggTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGa                                                                                                                                        >  1:747144/1‑149 (MQ=255)

           ggTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGGGAAAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGGATAGTCTCACCattgc                                                                                                                                        <  2:654936/149‑5 (MQ=255)

            gTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGCTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAg                                                                                                                                       >  1:81026/1‑149 (MQ=255)

               aaCAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCtt                                                                                                                                    <  1:1552694/149‑1 (MQ=255)

                 cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta                                                                                                                                  <  1:101422/149‑1 (MQ=255)

                 cAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTta                                                                                                                                  >  1:1347304/1‑149 (MQ=255)

                  aGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGTAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGAAGTGATAATCAGTTCCGGTTTGTGCTAACAGCGGGCAATGGTGAGACTATCCTCACCAGATAGCTTtat                                                                                                                                 >  2:177999/1‑149 (MQ=255)

                       cTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTc                                                                                                                            >  2:820790/1‑149 (MQ=255)

                          tAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCaaa                                                                                                                         <  2:124777/149‑1 (MQ=255)

                                 tGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGTTTTGAACTCAGTAAGAGCAGTGATAATAAGTTCCGGTTTGTGCTAAAATCGGGCAATTGTGAGACTATCCTAACCAGAGAGCTTTATACCTCAAAAACctct                                                                                                                  <  2:686651/149‑1 (MQ=255)

                                  tttttCGTTACACAATTTCAATCAAGTAGTCATTATGGCTGTTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                                 <  2:127455/148‑1 (MQ=255)

                                  gTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                                 <  2:500249/149‑1 (MQ=255)

                                  gTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                                 <  1:776819/149‑1 (MQ=255)

                                  gTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTg                                                                                                                 >  1:1375678/1‑149 (MQ=255)

                                   ttttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGc                                                                                                                >  2:408746/1‑149 (MQ=255)

                                    tttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACCCTCTGCg                                                                                                               <  2:782120/149‑1 (MQ=255)

                                    tttCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCg                                                                                                               <  2:176235/149‑1 (MQ=255)

                                          tACACAATTTCAATCAAGGAGTCATTATGGCTGGGTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAg                                                                                                         <  2:937315/149‑1 (MQ=255)

                                            cacaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAggg                                                                                                       >  2:522120/1‑149 (MQ=255)

                                             acaATTTCAATCAAGGAGTCATTTTGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACAAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGc                                                                                                      >  1:1094263/1‑149 (MQ=255)

                                             acaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAggtc                                                                                                      >  1:662607/1‑147 (MQ=255)

                                             acaATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGc                                                                                                      >  2:587892/1‑149 (MQ=255)

                                               aaTTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCAt                                                                                                    >  2:479696/1‑149 (MQ=255)

                                               aaTTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCAt                                                                                                    >  2:205193/1‑149 (MQ=255)

                                                 tttCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTGTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATcg                                                                                                  <  1:1099751/149‑1 (MQ=255)

                                                     aatcaaGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTACACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTc                                                                                              >  2:1469777/1‑149 (MQ=255)

                                                     aatcaaGGAGTCATTATGGCTGGCTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGGGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTc                                                                                              <  2:452438/149‑1 (MQ=255)

                                                      atcaaGGAGTCATTATGGCTGGTTGGGTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTGGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCg                                                                                             <  1:1199863/149‑1 (MQ=255)

                                                      atcaaGGAGTCATTATGGCTGGTTGGATTGAACTCAGTAAGATCAGTGAAAATCAGTTACGGTTTGTGCTAAAAGAGAGCAATTGTTAGACTATCCTCACCTTCTAGCTTTATAAATCAAAAACCTCTGCGGAAAAGCGCATAGCGtag                                                                                             >  1:311313/1‑147 (MQ=255)

                                                       tcaaGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCgg                                                                                            >  2:1209495/1‑149 (MQ=255)

                                                       tcaaCGAGTCATTATGTATTGTTGCTTTTATCGCATTAAGAGCAGTTATAAACACTTCCGGTTTTTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCgg                                                                                            <  1:1061737/149‑1 (MQ=255)

                                                          aGGAGTCATTATGGCTGGTTGGGTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACGCTCAGCGGAAAAGGGCCTCGCGTCGGTGc                                                                                         >  2:258866/1‑149 (MQ=255)

                                                              gTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAg                                                                                     >  1:1424524/1‑149 (MQ=255)

                                                              gTCATTATGGCTGGTTGGTTTGAACGCAGTAAGAGCAGTGTTAATCAGTTCCGGATTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCAGCGGAAAAGGGCATCGCGTCGGTGCGTAg                                                                                     <  2:1518819/149‑1 (MQ=255)

                                                               tCATTATGGCTGGGTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAACCCTCTGCGGAAAAGGGCATCGCGGCGGTGCGTAGc                                                                                    <  2:476733/149‑1 (MQ=255)

                                                                 aTTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCaa                                                                                  >  1:373182/1‑149 (MQ=255)

                                                                  ttATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAAc                                                                                 >  2:542926/1‑149 (MQ=255)

                                                                   tATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACa                                                                                <  1:426272/149‑1 (MQ=255)

                                                                     tggctggcTGGTTTGAACTAAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGc                                                                              <  1:273548/149‑1 (MQ=255)

                                                                       gCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGccc                                                                            <  1:1128753/149‑1 (MQ=255)

                                                                              ggttTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGCGCTTTATACCTCAAAAACCTCAGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCaagaa                                                                     >  2:270919/1‑149 (MQ=255)

                                                                               gttTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCaagaag                                                                    >  2:1475758/1‑149 (MQ=255)

                                                                                 ttGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAgaagaa                                                                  <  1:766651/149‑1 (MQ=255)

                                                                                  tGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAAc                                                                 <  1:1511557/149‑1 (MQ=255)

                                                                                   gAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACg                                                                <  2:457501/149‑1 (MQ=255)

                                                                                     aCTGAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATTTTGAGATTATACTCACCAGCGTGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCAGCGCGGCGGGGCGGAGCAACAGCCCGCAAGAAGCACGCt                                                              <  1:842626/149‑1 (MQ=255)

                                                                                       tCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTAt                                                            >  1:1264006/1‑149 (MQ=255)

                                                                                          gTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGAGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATgag                                                         >  2:609334/1‑149 (MQ=255)

                                                                                              gagCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGaaaa                                                     <  2:790328/149‑1 (MQ=255)

                                                                                                gCAGTGAGAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAATGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTAGGAGaaaaaa                                                   >  2:1322617/1‑149 (MQ=255)

                                                                                                  aGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAAc                                                 <  2:648053/149‑1 (MQ=255)

                                                                                                  aGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAAc                                                 <  2:163329/149‑1 (MQ=255)

                                                                                                   gTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGGAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACg                                                <  2:216210/149‑1 (MQ=255)

                                                                                                    tGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACgg                                               <  1:524141/149‑1 (MQ=255)

                                                                                                      aTAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCa                                             >  1:755315/1‑149 (MQ=255)

                                                                                                      aTAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTCCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCa                                             >  1:711613/1‑149 (MQ=255)

                                                                                                       tAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCaa                                            <  1:205193/149‑1 (MQ=255)

                                                                                                       tAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCaa                                            >  1:1130280/1‑149 (MQ=255)

                                                                                                        aaTCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAg                                           <  1:565266/149‑1 (MQ=255)

                                                                                                         aTCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGt                                          <  1:1244918/149‑1 (MQ=255)

                                                                                                          tCAGTTGCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTa                                         >  1:702714/1‑149 (MQ=255)

                                                                                                             gTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAATAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGAAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACg                                      <  2:1568256/149‑1 (MQ=255)

                                                                                                             gTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACg                                      >  2:1267954/1‑149 (MQ=255)

                                                                                                              ttCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACgg                                     >  1:34862/1‑149 (MQ=255)

                                                                                                                  ggTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCCTCTCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCaaa                                 >  1:1243546/1‑149 (MQ=255)

                                                                                                                  ggTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCaaa                                 <  1:777314/149‑1 (MQ=255)

                                                                                                                   gTTTGTGATAAAAGCGGGCAATGGTGAGACTATCCTCAACAGCGAGCTTTATACCTCAAAAACCTCTCCGGAAAAGGGCATAGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAAt                                <  1:1474030/149‑1 (MQ=255)

                                                                                                                    tttGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAAtt                               >  1:1340922/1‑149 (MQ=255)

                                                                                                                      tgtgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGGCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCt                             <  1:973455/149‑1 (MQ=255)

                                                                                                                        tgCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTAt                           >  2:1543465/1‑149 (MQ=255)

                                                                                                                         gCTAAAAGCGGGCAATGGTGAGACTATCCTCCCCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTAtt                          >  1:981323/1‑149 (MQ=255)

                                                                                                                          cTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTAATACCTCAAAACCCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGATAAAAAAGGCAAGTAACGGCAAATGCTAttt                         >  1:1521046/1‑149 (MQ=255)

                                                                                                                           tAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTc                        >  1:1557528/1‑149 (MQ=255)

                                                                                                                             aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCaa                      >  1:557296/1‑149 (MQ=255)

                                                                                                                             aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCaa                      >  1:1123288/1‑149 (MQ=255)

                                                                                                                             aaaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAAAGGCAAATTCTATTTCaa                      >  2:1387847/1‑149 (MQ=255)

                                                                                                                              aaGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAAt                     <  1:919608/149‑1 (MQ=255)

                                                                                                                               aGCGGGCAATGGTGAGACTTTCCTCACCAGCGAGCGTTATACCTCAAAAACCTCAGCGGAACAGGGCATCGCGGCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGATAAAAAAACGGCAAGTAAAGGCAAATTCTATTTCAATc                    <  2:1250076/149‑1 (MQ=255)

                                                                                                                                gCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCt                   >  2:762548/1‑149 (MQ=255)

                                                                                                                                gCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCt                   <  2:81026/149‑1 (MQ=255)

                                                                                                                                   ggCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACCGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTTTTTCAATCTGaa                >  2:154767/1‑149 (MQ=255)

                                                                                                                                    tcgctGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGaaa               >  1:1265655/5‑149 (MQ=255)

                                                                                                                                    gccaTGGTGAGACTATCCTCACCAGCTAGCTTTATACCTCAAAAAACTCTGCGGACACGGGCATCGCGTCGGTTCTTAGCAACAGCCCGCAAGAAGAACGCTATTATAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGaaa               <  2:1062357/146‑1 (MQ=255)

                                                                                                                                      gctGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGc             >  1:30282/3‑149 (MQ=255)

                                                                                                                                      aaTGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGc             >  2:1536059/1‑149 (MQ=255)

                                                                                                                                        tGGTGAGGATAGTCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAACCGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCg           >  2:78251/1‑149 (MQ=255)

                                                                                                                                         ggggagaCTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGc          <  2:1438551/146‑1 (MQ=255)

                                                                                                                                           tGAGACTATCCTCACCAGCGAGCTTTATACCTCAATAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACCGCCCGCAAGAAGCACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTa        >  2:202987/1‑149 (MQ=255)

                                                                                                                                           tGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAACCGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTa        >  2:1372298/1‑149 (MQ=255)

                                                                                                                                                ctctcctccCCAGCGAGCTTTAAACCTCAAAAACCTGTGCGGAACAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAatcat   <  2:568760/146‑1 (MQ=255)

                                                                                                                                                 tATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAatcatc  <  1:1209495/149‑1 (MQ=255)

ATACTTTAATCGGTAAACAGCACCTTTAGATGCTGTTTTCGATACACAATTTCAATCAAGGAGTCATTATGGCTGGTTGGTTTGAACTCAGTAAGAGCAGTGATAATCAGTTCCGGTTTGTGCTAAAAGCGGGCAATGGTGAGACTATCCTCACCAGCGAGCTTTATACCTCAAAAACCTCTGCGGAAAAGGGCATCGCGTCGGTGCGTAGCAACAGCCCGCAAGAAGAACGCTATGAGAAAAAAACGGCAAGTAACGGCAAATTCTATTTCAATCTGAAAGCCGCTAATCATC  >  NC_000913/2165121‑2165414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`