Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,242,571 T→A 5.8% N133Y (AAT→TAT)  yeiB ← DUF418 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,5710TA 5.8% 261.7 / 4.2 104N133Y (AAT→TAT) yeiBDUF418 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/47);  new base A (5/2);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 107 total aligned reads displayed.

GCTTCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATC  >  NC_000913/2242423‑2242719
                                                                                                                                                    |                                                                                                                                                    
gCTTCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTAtt                                                                                                                                                      <  1:1294702/149‑1 (MQ=255)
  ttCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTaa                                                                                                                                                    <  1:311978/149‑1 (MQ=255)
   tCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCCGCCCCAATAACAGCAAAACGCCACGCCCCACCAGATAAAGCATGACGCCGGTATTaca                                                                                                                                                   >  1:1163661/1‑147 (MQ=255)
   tCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                                                                                                                                   <  1:389789/149‑1 (MQ=255)
   tCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                                                                                                                                   <  1:1128889/149‑1 (MQ=255)
    cAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCGGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                                                                                                                                  <  1:1263782/149‑1 (MQ=255)
     aaCGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACa                                                                                                                                                 >  1:854319/1‑149 (MQ=255)
        gccgccGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAACACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAggg                                                                                                                                              >  2:1130843/1‑148 (MQ=255)
         ccgccgTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGGATGACGCCTGTATTAAACAGGCt                                                                                                                                             >  1:6788/1‑149 (MQ=255)
          cgccgTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATCCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCACCAGCAGAGAATGCATGACGCCTGTATTAAACAGGCtt                                                                                                                                            >  2:1266461/1‑149 (MQ=255)
            ccgTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTTTAATACAGGcgtca                                                                                                                                          <  1:1213129/149‑5 (MQ=255)
             cgTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGTCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAACAGCCAAGCCCCACCAGATAAATCATGACGCCTGTATTAAACAGGCttttt                                                                                                                                         >  2:353127/1‑149 (MQ=255)
             cgTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGAAGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTTTAATACAGGcgtcat                                                                                                                                         <  2:118130/149‑6 (MQ=255)
              gTGATGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATACAGCATGACGCCTGTATTAAACAGGCTTTTTa                                                                                                                                        <  2:529368/149‑1 (MQ=255)
               tGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                                                                                                                                       <  2:349892/149‑1 (MQ=255)
                gAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAACAAACCGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAcc                                                                                                                                      >  2:777292/1‑149 (MQ=255)
                   gCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCATGCGCGGCAGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAATCCGGCAAGACCCACCAGAAAAAGCATGACGCCGGTATTAACCAGGCTTTTTACCTAt                                                                                                                                   >  2:145588/1‑149 (MQ=255)
                           tATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                                                                                                                           <  2:1453610/149‑1 (MQ=255)
                              ttatCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg                                                                                                                        <  2:1003557/146‑1 (MQ=255)
                               tttCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgc                                                                                                                       <  1:288035/149‑1 (MQ=255)
                                ttCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCgcgcc                                                                                                                      >  1:1192416/1‑148 (MQ=255)
                                   aTACAAAATAGCCGATGCAACCGGCGACAAGGCAAGGCAGGTCTGCATGACGGAAATCAGCACCAATAAAAGCAAAACCCCTATCCACACCACATAAAGCAAGACGCCTGTATAAAACACGCATTTTCCCGCTGGCGCATCGAGCACCa                                                                                                                   >  2:1327957/1‑149 (MQ=255)
                                     aCAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACCGCAAAACGCCAAGCCCCCCCAGATAAAGCCTGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCCTCGCGCACCAgc                                                                                                                 >  1:1162502/1‑149 (MQ=255)
                                      cAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATTAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc                                                                                                                <  2:1428058/149‑1 (MQ=255)
                                      cAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc                                                                                                                <  2:1311754/149‑1 (MQ=255)
                                      cAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc                                                                                                                >  2:466744/1‑149 (MQ=255)
                                      cAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGCTCTGGTTGACGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCCCCAGATAAAGCATCACTCCTGGATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc                                                                                                                >  2:1349777/1‑149 (MQ=255)
                                       aaaaTAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg                                                                                                               >  1:1541555/1‑149 (MQ=255)
                                         aaTAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCACAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGGAGTAAACAGGCGTTTTACCGATGGCGCATCGCGCACCAgccgcc                                                                                                             >  1:1327421/1‑149 (MQ=255)
                                              ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTTCCGATGGCGCATCGCGCACCAGCCGCcagcag                                                                                                        >  2:171368/1‑149 (MQ=255)
                                                     aTCCGGCGTCCAGGCGCGGCTTGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGc                                                                                                 <  1:184173/149‑1 (MQ=255)
                                                       ccGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCACGCCCCACCAGATAAAGCATGACGCCGGTATTAAACCGGCTTTTTACCGATGGCGCCTCGCGCCCCCGCCGCCAGCAGATTAAGccc                                                                                               >  1:146369/1‑149 (MQ=255)
                                                        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAACGCAGGACGCCTGTATTAAACAGGCTTTTTACCGATGGCTCATCGCGCACCAGCCGCCAGCAGATTAAGCcca                                                                                              <  2:342012/149‑1 (MQ=255)
                                                        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGcccc                                                                                              >  2:1540696/1‑148 (MQ=255)
                                                          gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCccacc                                                                                            >  2:303015/1‑149 (MQ=255)
                                                            gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCCAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTGTGACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAcgcccgcccg                                                                                          >  2:443592/1‑147 (MQ=255)
                                                             tCCAGCCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGc                                                                                         >  2:294829/1‑149 (MQ=255)
                                                                aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCg                                                                                      >  1:678433/1‑149 (MQ=255)
                                                                 ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAGCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGt                                                                                     <  1:337279/149‑1 (MQ=255)
                                                                 ggCGCGGCTGGTCTGGCTGTCGGAAATCAGCCACAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACCTGCTTTTTACCGATGGCGCATCGCGCACCAGCCGACAGAAGATTAAGCCCACCAGCCCGt                                                                                     >  2:88643/1‑149 (MQ=255)
                                                                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCCCCAGATAAAGCATGACGCCTGTATTACACAGGCTGTTTACCGATGGCGCATCGCGCACCAGCCGCCCGCAGCTGAAGCCCCCCAGCCCGTAAGCCAGcc                                                                            >  2:384066/1‑148 (MQ=255)
                                                                           gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCCCCAGATAAAGCATGACGCGTGTATTAAACAGGCTTTTTACCGATGGCTCATCGCGCACCAGCCGCCAGCAGATTTAGCCCACCAGCCCGTAAGCCAGcta                                                                           >  1:1024316/1‑147 (MQ=255)
                                                                              tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATAAAACAGGCTTTAAACCGATGGCGCATCGCGCACCAGCCGCGCGCAGATTAAGGCCACCGGGCGGTAAGCAAGCaaatt                                                                        >  2:782255/1‑147 (MQ=255)
                                                                              tGGCTGTCGGAAATCAGCACCAATAACAGAAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTGTTTACCGATGGCGCCACGCGCACCAGCCGCCAGCAGATTAAGCCACCCTGCCCGTAAGCCAGCGAAat                                                                        >  1:905791/1‑149 (MQ=255)
                                                                                  tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCg                                                                    <  1:1220333/149‑1 (MQ=255)
                                                                                  tGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCg                                                                    <  1:9190/149‑1 (MQ=255)
                                                                                   gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGAATAAGCCCCCCAGCCCGTAAGCCCGCAAAATATCGc                                                                   >  2:9190/1‑149 (MQ=255)
                                                                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGt                                                                <  1:1239382/149‑1 (MQ=255)
                                                                                          aTCAGCCGCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGAGGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCa                                                            <  1:971572/149‑1 (MQ=255)
                                                                                           tCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCATTTTACCGCTGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCCGCAAAATATCGCCCTCCCat                                                           >  1:311346/1‑148 (MQ=255)
                                                                                             aGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGaa                                                         <  1:149983/149‑1 (MQ=255)
                                                                                               ccccAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACa                                                       <  1:294829/149‑1 (MQ=255)
                                                                                               ccccAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGAACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACa                                                       <  1:710951/149‑1 (MQ=255)
                                                                                                cccAATAACAGCACAACGCCAAGCCCAACCAGATAAAGCATGACGCCTGTATTAAACACGCTTATTACCGATGGCGCATCGCGCACCAGCCGCCAGCACATTAAGCCCCACAGCCAGTAAGCCAGCAATATATCGCCGTCCCAGAACaa                                                      >  1:1176025/1‑149 (MQ=255)
                                                                                                 ccAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCGTCGCGCACCAGCCGCCAGCTGATTAAGCCACCCAGCCCGTAAGCCAGCAAATAATAGCCGGCCCAGAACAAt                                                     >  1:355334/1‑149 (MQ=255)
                                                                                                 ccAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCGTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCCGCCCGTAAGCCAGCAAAATATCGACGTCCCCGAACAAt                                                     >  1:295724/1‑149 (MQ=255)
                                                                                                  cAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTTAGCCAGCAAAATATCGCCGTCCCAGAACAATa                                                    >  2:757020/1‑149 (MQ=255)
                                                                                                  cAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATa                                                    <  2:1541555/149‑1 (MQ=255)
                                                                                                   aaTAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATaa                                                   >  1:1370660/1‑149 (MQ=255)
                                                                                                    aTAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATaaa                                                  <  2:1280649/149‑1 (MQ=255)
                                                                                                     tAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAAc                                                 <  1:1146133/149‑1 (MQ=255)
                                                                                                       aCAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGCTGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCg                                               >  2:245901/1‑149 (MQ=255)
                                                                                                        cAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGt                                              <  1:195899/149‑1 (MQ=255)
                                                                                                        cAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCGGTATTAAACAGGCTTTTTACCGCTGGCGCATCGCGCACCAGCCGCCAGCAGAGTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGGCCCAGAACAATAAACCGt                                              <  2:854319/149‑1 (MQ=255)
                                                                                                        aaGCAAAACGACAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGATTTTTACCGATGGCGCATCGCGCACCAGCCGCCGGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGt                                              <  2:1077854/148‑1 (MQ=255)
                                                                                                         aGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGCCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCggg                                             >  1:720641/1‑147 (MQ=255)
                                                                                                          gCAAAACTCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGa                                            <  1:1346013/149‑1 (MQ=255)
                                                                                                             caaCGCCAAGCCACACAAGATAAAGCATGACGCCTGTATTAAAAAGGCTGTTTACCGATGGCGCCTCGCGCACCAGCCGCGAGCAGATTACGCCCAGAGGCCAGTAAGCTCGCAAACTATCGCCGGCCCAGCGCCAGTAGCCGTGAATa                                         >  2:779533/2‑149 (MQ=255)
                                                                                                               aCGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATaaa                                       <  1:148930/149‑1 (MQ=255)
                                                                                                                cGCCTAGCCCCACCAGATAAAGAATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGAATCGCGAACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAg                                      <  1:490056/149‑1 (MQ=255)
                                                                                                                cGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAg                                      >  1:1390442/1‑149 (MQ=255)
                                                                                                                    aaGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCCGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAAAAAACCGTGAATAAAGCCca                                  >  1:63072/1‑149 (MQ=255)
                                                                                                                     aGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaa                                 <  2:1545034/149‑1 (MQ=255)
                                                                                                                      gCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaac                                >  2:712114/1‑149 (MQ=255)
                                                                                                                        cccACCAGATACAGCATCACGCCTGTATTAACCAGGCTTTTTACCGATGGCGCAGCGCTCACCAGCCGCCAGCAGAATAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCcaacca                              <  2:1463992/149‑3 (MQ=255)
                                                                                                                         ccaccaGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAg                             <  1:1540696/149‑1 (MQ=255)
                                                                                                                          caccaGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGAAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAACCCAAACAAGa                            >  1:1133448/1‑149 (MQ=255)
                                                                                                                          caccaGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATAGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACAGTGAATAAAGCCCAACAAGa                            >  2:154205/1‑149 (MQ=255)
                                                                                                                           cccaGATAAAGCATGACGCCTGTCTTAAACAGGCTGTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGGCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGAc                           <  2:417178/148‑1 (MQ=255)
                                                                                                                              aGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGGCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTaa                        <  1:895856/149‑1 (MQ=255)
                                                                                                                                  ataGCATGACGCGTGTTTTAAACCGGGTTTTTACGGATGGCGCATCGCGCAGCAGCCGCCAGCAGATTAAGCCCACCAGCACGTAAGGCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGc                    <  2:311346/147‑1 (MQ=255)
                                                                                                                                  aaaGCATGACGCCTGTATTAAACAGGCTTTTTACAGATGGCGCATCGAGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGc                    <  1:353127/149‑1 (MQ=255)
                                                                                                                                     gCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCGGTCCCAGAACAATAACCCGTGAATAAAGCCAACCAACCCTACCAGCGtt                 >  2:1375540/1‑149 (MQ=255)
                                                                                                                                     gCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGGCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGtt                 >  1:398833/1‑149 (MQ=255)
                                                                                                                                     ccctGACGCCTTTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACTCGCCCCCAGCAGTTTAATCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGtt                 <  2:1424882/146‑1 (MQ=255)
                                                                                                                                       aTGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTaa               <  1:466744/149‑1 (MQ=255)
                                                                                                                                       aTGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCACACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAACGCCCAACAAGACTAACAGCGTTaa               <  1:1552441/149‑1 (MQ=255)
                                                                                                                                       aTGACGCCTGTATT‑AACAGGCTTTTTACCGATGGCGCATCTCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAAc              <  1:1096456/149‑1 (MQ=255)
                                                                                                                                        tGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATAAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAAc              <  1:1175625/149‑1 (MQ=255)
                                                                                                                                        aaaagCCTGTTTAATACAGGCTTTTTACCGATGGCGCATCGCGCACCCGCCGCCAGCAGATTACGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATACACCGTGAAGAAAGCCCAACAAGACTAACCGCGTTAAc              >  2:760778/5‑149 (MQ=255)
                                                                                                                                        aaaagCCTGTTTAATACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAAc              >  2:691855/5‑149 (MQ=255)
                                                                                                                                          aCGCCTGTATTAAACAGGCTTTTTACCGATGGCGCCTCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAAGAAACCGTGCATAAAGCCCAACAAGACTACCAGCGTTAcccc            >  1:1126933/1‑148 (MQ=255)
                                                                                                                                           agCCTGTTTAATACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCTCCAGCAGATTAAGCCCTCCAGCCCGTACGCGCGCAAAATATCGCCGACCCAGAACAATAAGCCGTGAATAAAGCCCAACAAGACGAACAGCGTTACCcgc           >  1:888961/2‑149 (MQ=255)
                                                                                                                                            gCCAGTATTAAACAGGCTTTTTACCGATGGCGCAACGCGCACCAGCCGCCAGCAGATTAAGCACACAAGACCGTAAGCCAGCAATATATCGAAGTCCCAGAACAATAACCAGTGAATTAAGCCCAAAAAGACTAACAGCTTTAACcgcc          >  2:158120/1‑148 (MQ=255)
                                                                                                                                              cTGTATTAAACAGGCTTTTTACCGATTGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGAc        <  2:1327421/149‑1 (MQ=255)
                                                                                                                                               tGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACt       <  1:757020/149‑1 (MQ=255)
                                                                                                                                               tGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACt       >  1:469463/1‑149 (MQ=255)
                                                                                                                                                 tATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTTAGCCCACCAGCCCGTAAGCCAGCAATATATCGCCGTCCCAGCACAATAAAACGTGAATAAAGCCCAGCAAGACTAACAGCGTTTACCGCGAATgg     >  2:1208623/1‑149 (MQ=255)
                                                                                                                                                 tATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGACCCAGAACAATAAACCGTGAATAACGCCCCACAAGACTAACAGCGTTAACCGCGCCTgg     >  1:825089/1‑149 (MQ=255)
                                                                                                                                                    tAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCGACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATc  <  1:147898/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTCAACGCCGCCGTGAAGCTTCCAGTATTTTTCATACAAAATAGCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCAGCAGATTAAGCCCACCAGCCCGTAAGCCAGCAAAATATCGCCGTCCCAGAACAATAAACCGTGAATAAAGCCCAACAAGACTAACAGCGTTAACCGCGACTGGATC  >  NC_000913/2242423‑2242719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑