Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,619 A→G 5.1% intergenic (‑85/+27) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6190AG 5.1% 227.4 / 2.7 78intergenic (‑85/+27)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (32/42);  new base G (2/2);  total (34/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

GATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG  >  NC_000913/2311471‑2311766
                                                                                                                                                    |                                                                                                                                                   
gATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCa                                                                                                                                                     <  1:623006/149‑1 (MQ=255)
  tCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAgg                                                                                                                                                   >  1:704914/1‑149 (MQ=255)
      aaccaacAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCt                                                                                                                                               <  1:1108644/149‑1 (MQ=255)
        ccaaaAAAGCAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCCTACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCttt                                                                                                                                             <  2:1384331/149‑1 (MQ=255)
          aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                           <  2:863703/149‑1 (MQ=255)
          aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGt                                                                                                                                           >  1:1290685/1‑149 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                          <  2:738928/149‑1 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                          <  2:1534177/149‑1 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                          <  1:94077/149‑1 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                          <  1:386050/149‑1 (MQ=255)
           acaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGAGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCACGCAGGCCCTTTGtt                                                                                                                                          >  1:1021241/1‑149 (MQ=255)
           acaaAGAAGAGAGCGTCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATAATCCTCATGCGAAAGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAAGAATGATAAAAGGGCCCGCAGGCCCTTTGtt                                                                                                                                          <  2:686536/149‑1 (MQ=255)
            caaTGACGAGCGCGGCCGACATCAGTGCCACGCGGGTAAAGCTTATATCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                                                                         <  2:537932/149‑1 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCTTTTTTc                                                                                                                                         <  2:187854/149‑1 (MQ=255)
            caaAGAAGAGCGCGGCAGCCAGCACTGCCACACGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCACGCACGCCCTttcttc                                                                                                                                         >  2:996500/1‑149 (MQ=255)
            aaaaGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCTAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                                                                         <  1:920057/148‑1 (MQ=255)
            aaaaGAAGAGCGCGGCAGACAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCTCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTc                                                                                                                                         <  2:1025570/148‑1 (MQ=255)
              aagaagAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                                       <  2:1340419/149‑1 (MQ=255)
               agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                                                                      <  1:1500455/149‑1 (MQ=255)
               agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTGTGTACGCTGAAAAGAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGat                                                                                                                                      >  2:297574/1‑149 (MQ=255)
                tacgagCGCGGCAGCCAGCAGTGCCACGCGTGAAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGGCGCAAGAGTACACCAAAAATCTGAGTTTGTACGCTGAAAACAATGAACAAAGGGCCTGCGGGCCCTTTTTTcattg                                                                                                                                     <  1:137211/146‑5 (MQ=255)
                   gagCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCa                                                                                                                                  <  1:813286/149‑1 (MQ=255)
                         ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgag                                                                                                                            <  1:1313628/149‑1 (MQ=255)
                             gccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCTATATCAATCGAGATTa                                                                                                                        >  1:1417082/1‑149 (MQ=255)
                              ccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAg                                                                                                                       <  2:602689/149‑1 (MQ=255)
                                    gTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTAGATATCAATCGAGATTAGAACTgg                                                                                                                 <  2:1158817/149‑1 (MQ=255)
                                        cACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                                             <  2:3557/149‑1 (MQ=255)
                                             gggTATAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAATCTGACTTTGTACGCTGAAAACTATGAAATAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGTTTAGAACTGGTAAACCAGa                                                                                                        <  2:279254/149‑1 (MQ=255)
                                             gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATc                                                                                                                               >  2:804738/1‑126 (MQ=255)
                                             gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATc                                                                                                                               <  1:804738/126‑1 (MQ=255)
                                                     cTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGCCCCAgcgc                                                                                                >  2:275155/1‑149 (MQ=255)
                                                         tttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACg                                                                                            >  1:1397978/1‑149 (MQ=255)
                                                          ttCCATACGTGATTTTCCTCATGCGAAGGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGa                                                                                           <  1:200517/149‑1 (MQ=255)
                                                          ttCCATACGGGATTATCCTCATGGGATCGGTCGCAAGTGTACACCTAAAAACTGAGTTTGTAGGCTGAAATCATTGAAAAAAGGGCCCGGAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGa                                                                                           <  1:206591/149‑1 (MQ=255)
                                                            ccATACGTGATTATCCTCATGCGAACGGGCGCAAGAGTACACCAAAAAAGTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCACTCGAGATTAGAACTGGGAAACCAGACCCAGAGCTACGATg                                                                                         <  1:278547/149‑1 (MQ=255)
                                                                  gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAGGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                   >  2:1109149/1‑149 (MQ=255)
                                                                  gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                   <  2:1230932/149‑1 (MQ=255)
                                                                  gTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCa                                                                                   <  1:267726/149‑1 (MQ=255)
                                                                    gATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                 >  2:330009/1‑149 (MQ=255)
                                                                    gATTATCCTCATGCGAACGGTCGCAAGAGTACAACAAAAAACTTAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgt                                                                                 <  1:872626/149‑1 (MQ=255)
                                                                      ttATCCTCAAGCGAACGGGCGCAAGAGTACACCAAAAAACTGAGTTTGAACGCTGAAACCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTATACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                               <  2:1021241/149‑1 (MQ=255)
                                                                       tATCCTCATGAGAACGTGCGCAATATTAAACAACACAACTGAGATTGTACGCTGAAAAAAAAGAAAAATTGGCCCGCAGTCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGtt                                                                              <  2:1085788/149‑1 (MQ=255)
                                                                           cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                          <  2:1528970/149‑1 (MQ=255)
                                                                            acATGCCAACGGTCGCCAGAGTACCCCAAAAACCTGAGTATCTACGCTGAAAACCATGCAAACAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                         <  1:1144584/148‑1 (MQ=255)
                                                                              aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                       >  2:201703/1‑149 (MQ=255)
                                                                              aTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCa                                                                       <  2:1285200/149‑1 (MQ=255)
                                                                               tGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAg                                                                      >  1:46711/1‑149 (MQ=255)
                                                                                   aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTc                                                                  <  2:704914/149‑1 (MQ=255)
                                                                                    aCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTATGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTcc                                                                 >  1:1319771/1‑148 (MQ=255)
                                                                                        tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACTATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAg                                                             <  1:1212679/149‑1 (MQ=255)
                                                                                           cAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGa                                                          <  2:111178/149‑1 (MQ=255)
                                                                                            aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                         >  1:687722/1‑149 (MQ=255)
                                                                                                gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTgg                                                     <  2:293131/149‑1 (MQ=255)
                                                                                                 tACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGTTTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGt                                                    <  1:1406832/149‑1 (MQ=255)
                                                                                                  acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGCGCCCGCAGGCCCGTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                   <  2:450470/149‑1 (MQ=255)
                                                                                                    acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCACGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                 >  1:578184/1‑149 (MQ=255)
                                                                                                      cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                               >  2:1537331/1‑149 (MQ=255)
                                                                                                       aaaaaaCTGAGTTTGTATGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCAAGCGTCACGAGTGAACTGGTTgtcgt                                              >  1:692632/1‑149 (MQ=255)
                                                                                                              tGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagcag                                       >  1:1302339/1‑149 (MQ=255)
                                                                                                                 gaatGTACGGTGAAACCAATCAAACAAGGGCCCGCAGGCCAATAGTTAGATATCAATCGAGAGTAGAAATGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtg                                    <  2:1040095/146‑2 (MQ=255)
                                                                                                                 gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAACAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCAAGCAGGtt                                    >  1:714188/1‑149 (MQ=255)
                                                                                                                       aCGCTTAAACCAAAGAAAACAGGTCCCGCAGGCCCTATGTTCGATATCAATCGACATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGGGTTGATGCCAGCGTCACGAGGGAACTGGTTGTCGTCCAGCAGGTTGAtttt                              <  2:241649/149‑1 (MQ=255)
                                                                                                                         gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                            >  1:110587/1‑149 (MQ=255)
                                                                                                                          cTGAAAACAATGAAACAAGGGCCCGCAGTCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGATCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                           <  2:1417082/149‑1 (MQ=255)
                                                                                                                          cTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTGTGTTCGATATCAATCGAGATTAGAACTGTTAACCCAGACCCAGAGCTACGATGTGATGAGTGTTGATGCGAGGGTCACGAGTGACCGGGTTGGCGTCCAGCAGGGTGAGTTTGtt                           >  1:1564630/1‑148 (MQ=255)
                                                                                                                             caaaCAATGATAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGCTTAGAACTGGTATACCAGCCCCAGCTCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCCGGTTGATTTTGTAGTc                        >  2:1024969/2‑149 (MQ=255)
                                                                                                                               aaCAATGAAAATAGGGCCCGAAGGCCCTTTGTTCGATATCAATCGAGATTATAACTTGTAAACCATACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTTACGAGGGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTaaa                      <  2:1100458/149‑1 (MQ=255)
                                                                                                                                  aaTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                   >  2:594271/1‑149 (MQ=255)
                                                                                                                                   aTGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                  >  1:369050/1‑149 (MQ=255)
                                                                                                                                     gAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                >  1:688661/1‑149 (MQ=255)
                                                                                                                                     gAACAAAGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                >  1:1470413/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt               >  1:889449/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt               >  1:889448/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt               >  1:37927/1‑149 (MQ=255)
                                                                                                                                      aaaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt               >  1:179298/1‑149 (MQ=255)
                                                                                                                                             ggCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATg        >  2:1309843/1‑149 (MQ=255)
                                                                                                                                              gCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAACCCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt       >  1:396402/1‑149 (MQ=255)
                                                                                                                                                  gCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGGACTGGTTGTCGACCAGCAGTTAGATTTTGTAGTCAACGTAGGTGGACATGttttt   >  2:970547/1‑149 (MQ=255)
                                                                                                                                                   cAGTCCCTTTGTTCGATAACAATCCAGATAAGAACTGGTAACCCACACCCATAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGAGGACATGTTTttg  <  2:1138103/149‑1 (MQ=255)
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GATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTG  >  NC_000913/2311471‑2311766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: