Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,354,318 C→T 5.7% G18G (GGC→GGT glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,354,3180CT 5.7% 235.2 / 3.3 87G18G (GGC→GGTglpBanaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base C (37/44);  new base T (2/3);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGC  >  NC_000913/2354170‑2354466
                                                                                                                                                    |                                                                                                                                                    
cGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGc                                                                                                                                                      <  2:943763/149‑1 (MQ=255)
   gCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGTGATTGTGTGGTCTGGAGAAGGAGCAGAAATATGCGCTTTGATACTGGCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctg                                                                                                                                                   <  2:90981/149‑1 (MQ=255)
    cAACCCCTCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGGCTGGAGAAGGAGCAGAAAGAGGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgc                                                                                                                                                  <  2:1472693/149‑1 (MQ=255)
     aaCCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgca                                                                                                                                                 >  2:723285/1‑149 (MQ=255)
       cccATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgcaac                                                                                                                                               >  1:1154918/1‑149 (MQ=255)
         cATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgcaactg                                                                                                                                             >  2:1568677/1‑149 (MQ=255)
          aTCGCCTGGGGAGATGCACTGCGCGAAAGCTAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACAGTCATTATGGGCGGCGGCCTCGCCGTATTACTCTGTGGCctgcaactgc                                                                                                                                            <  1:270346/149‑1 (MQ=255)
          aTCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGAGCTATGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgcaactgc                                                                                                                                            >  1:529890/1‑149 (MQ=255)
                tGGGGCGATGCACTGAGCGCACGCGAATTTACCCGCTGGGTTTATCCGGGATTGTGTGGTCTGGAGATGGAGCAGAATGTTGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCATCTGCAAAAac                                                                                                                                      <  2:1074107/149‑1 (MQ=255)
                 ggggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAaca                                                                                                                                     >  1:485199/1‑149 (MQ=255)
                 ggggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAaca                                                                                                                                     <  1:1426261/149‑1 (MQ=255)
                  gggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGCCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAacac                                                                                                                                    >  1:1528115/1‑149 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgc                                                                                                                                                  >  1:1075285/1‑134 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgc                                                                                                                                                  <  2:1075285/134‑1 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgc                                                                                                                                                  >  1:1170197/1‑134 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCctgc                                                                                                                                                  <  2:1170197/134‑1 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACg                                                                                                                                   <  1:591772/149‑1 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACg                                                                                                                                   >  1:1477794/1‑149 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACg                                                                                                                                   >  2:825783/1‑149 (MQ=255)
                   ggAGATGCACTGCGCGAAAGCGAATTTACACGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGAGCTTTGATACTGTCATTATGGGAGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACg                                                                                                                                   >  1:125376/1‑149 (MQ=255)
                          cACTGCGCGAAAGCGAATTTACCCGCTGGTGTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAACGATGAGCTTTGATACTGTCTTTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTgcg                                                                                                                            <  1:1263167/149‑1 (MQ=255)
                          cACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTgcg                                                                                                                            <  2:168459/149‑1 (MQ=255)
                            cTGCTCGAAAGCGAATTTACCCGCTGCGTTTATCAGGGATTGAGTGGACTGGACAAGGAGCAGAAAGATGCGCTATGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCAGTGGTCTGCAACTGCAAAAACACGTAATGCGCt                                                                                                                          >  1:789289/1‑149 (MQ=255)
                            cTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGAGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGTCTGCAACTGCAAAAACACGGCCTGCGCt                                                                                                                          >  1:795574/1‑149 (MQ=255)
                               cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCtgtg                                                                                                                       <  2:737652/149‑1 (MQ=255)
                                    acgCGAATTTACCCGATGGGTTTATCAGTGATTTTGTGGTCTTGATAAGTAGCATAAAGATGCGATTTGATACTGGCCTTTGTGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCAtt                                                                                                                  <  1:861412/147‑1 (MQ=255)
                                    aaGCGAATTTACCCTCTGGGTTTTTCAGGGATTGTTTTGTCTGGAGTAGGAGCATAAAGATGAGCTTTGATACTTTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGTCTGCAAATGCAAAAACACGGCCTGCGCTGTGCCAtt                                                                                                                  <  1:922200/149‑1 (MQ=255)
                                         aaTTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCAc                                                                                                             <  2:965245/149‑1 (MQ=255)
                                                 cGCTGGGTTTATCAGGGATTGTGTGGGCTGGAGAAGGAGCAGAAAGATGCGCTTTGATTCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTc                                                                                                     <  2:1282990/149‑1 (MQ=255)
                                                        tttATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAgcgc                                                                                              <  1:1377399/149‑1 (MQ=255)
                                                           aTCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTGGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACt                                                                                           >  1:6193/1‑149 (MQ=255)
                                                              aGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATAGTCACTCGTGGTCATAGCGCACTGCa                                                                                        >  1:1059906/1‑149 (MQ=255)
                                                               gggATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCAt                                                                                       >  2:441413/1‑149 (MQ=255)
                                                                  aTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTtc                                                                                    <  2:1477794/149‑1 (MQ=255)
                                                                        gggTCTGGAGAATGAGCAGCAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCGGGATTACTGTGTGGCGTGCATCTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATcc                                                                              <  2:1500241/148‑1 (MQ=255)
                                                                          gTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCgg                                                                            >  2:1567151/1‑149 (MQ=255)
                                                                          gTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAAAACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATACgg                                                                            >  2:1182496/1‑149 (MQ=255)
                                                                           tCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCggc                                                                           >  2:366410/1‑148 (MQ=255)
                                                                             tGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATc                                                                         >  1:807014/1‑149 (MQ=255)
                                                                              ggAGAAGGAGCAGAAAGATGCGCTTTGATGCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCg                                                                        >  2:214032/1‑149 (MQ=255)
                                                                               gagaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGc                                                                       >  2:261834/1‑149 (MQ=255)
                                                                                 gaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTg                                                                     <  1:302449/149‑1 (MQ=255)
                                                                                   aGGAGCAGAAAGAGGCGCTTTGAAACTGGCATATTGTGCGGCGGCCTCGCCGGATTACGCTGATGCCAGCAACTGCAACAACACGTCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGAGCGCTGGa                                                                   <  2:1076306/149‑1 (MQ=255)
                                                                                      ctcAGAAAGATGCGCTTTGATACTTTAATTATGGGCGGCTGCCTCGCCGGATTACTATGTGGCCGGCAACTGCAAAATCACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGAttt                                                                <  1:336421/147‑1 (MQ=255)
                                                                                       gCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTg                                                               >  2:739540/1‑149 (MQ=255)
                                                                                        cAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGc                                                              <  2:643324/149‑1 (MQ=255)
                                                                                        cAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGGGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGc                                                              <  2:1201601/149‑1 (MQ=255)
                                                                                         aGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCt                                                             <  2:485199/149‑1 (MQ=255)
                                                                                          gAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTg                                                            >  1:819942/1‑149 (MQ=255)
                                                                                                     gttGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGGGCCATTGTCACTCGTGGGCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGcc                                                 <  2:1376663/148‑1 (MQ=255)
                                                                                                      ttGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCa                                                <  2:1548598/149‑1 (MQ=255)
                                                                                                       tGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAg                                               >  1:872070/1‑149 (MQ=255)
                                                                                                        gATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGa                                              >  2:184841/1‑149 (MQ=255)
                                                                                                                 aTTATGGGCGGCGGCCGCGCCGGATTACGCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGGCAGATggctcagc                                     <  1:121997/149‑7 (MQ=255)
                                                                                                                  ttctGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCg                                    <  2:1376624/146‑1 (MQ=255)
                                                                                                                   tacgggCGGCGGCCTCGCCGGATTACTCTGTTGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGCGCGCTGGATTTGCTGAGCCATCTGCCAGATGGGCAACCgg                                   <  2:1036888/146‑1 (MQ=255)
                                                                                                                    aTGGGCGGCGGCCTCGCCGGATTCCTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGt                                  <  2:1241245/149‑1 (MQ=255)
                                                                                                                    aTGGGCGGCGGCCACGCCGGATTACGCTGTCGACTGCACCTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGAGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGt                                  <  2:1035286/149‑1 (MQ=255)
                                                                                                                      gggCGGCGGCCTCGCCGGAGTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                <  1:77350/149‑1 (MQ=255)
                                                                                                                        gcggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGCTGGTCACCCGGTgaca                              >  2:432654/1‑149 (MQ=255)
                                                                                                                            cggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgacagaca                          <  2:1154918/149‑1 (MQ=255)
                                                                                                                              gcttCGGCGGATTACTCTGTGGTCTGCAACTGCAAAAACAAGGCCTGCGCTTTTCCATTGTGACGCGTGGTCAAAGCTCACTTCTTTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGTTCAACCGGTGACAGTCAtt                        <  2:789289/146‑1 (MQ=255)
                                                                                                                              gcCTCGGCGGTTTACTCTGTGGTCTGCAACTTCAAAATCTCGGCCTGCGCTTTGCCATTGTCTCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACAtt                        <  2:795574/149‑1 (MQ=255)
                                                                                                                              gcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCCCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGCCAGACAtt                        >  1:1119955/1‑149 (MQ=255)
                                                                                                                              gcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACAtt                        >  2:970436/1‑149 (MQ=255)
                                                                                                                               ccgcGCCGGAGTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTc                       <  1:1395635/146‑1 (MQ=255)
                                                                                                                               ccTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTc                       <  1:441413/149‑1 (MQ=255)
                                                                                                                                cTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTca                      >  1:1147451/1‑149 (MQ=255)
                                                                                                                                cTCGCCGGATTACTCTGTGGCCTCCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATATCACATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATCGACAACCGGTgacagacaaaca                      >  1:388314/1‑145 (MQ=255)
                                                                                                                                 tCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCAATGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCTCTGGATATGCTGCGCCATCTCCCAGATGCTCACCCGGTGACAGACATGcac                     >  2:194189/1‑149 (MQ=255)
                                                                                                                                  cGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACCCGGCCTGCGCTGTGCCATTGTCACTCGTTGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                    <  2:288137/149‑1 (MQ=255)
                                                                                                                                   gCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAg                   <  2:1213580/149‑1 (MQ=255)
                                                                                                                                    ccGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGt                  <  2:807014/149‑1 (MQ=255)
                                                                                                                                    ccGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCGGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGt                  <  2:1392616/149‑1 (MQ=255)
                                                                                                                                        aTTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGAc              <  2:819942/149‑1 (MQ=255)
                                                                                                                                         ttACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCACAGCGCACTGCCTTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACt             <  2:316152/149‑1 (MQ=255)
                                                                                                                                         ttACGCTGTGGCGTGCAACAGCAAAAACTCGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATTGTCAACCGGTGACAGACATTCACAGTGGACt             <  2:529890/149‑1 (MQ=255)
                                                                                                                                          tACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGGGGTCAAAGCGCACTGCATTTCTCATCCGGAGCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTg            <  2:125376/149‑1 (MQ=255)
                                                                                                                                           aCTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTgg           <  1:825783/149‑1 (MQ=255)
                                                                                                                                              ctGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCCCAGTGGACTGGAAt        >  2:491326/1‑149 (MQ=255)
                                                                                                                                               tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCTCTGGATTTCTCATCCGGTTCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGGGGCTGACATTCGCCGTGGACTGGaagc       >  1:1185869/1‑147 (MQ=255)
                                                                                                                                               tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGCTTTGCTGAGCCATCTGCCAGATGGTCTCCCGGTGACAGACATTCACAGTGGACGGGAAta       >  1:1179908/1‑148 (MQ=255)
                                                                                                                                               tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATc       >  2:1112055/1‑149 (MQ=255)
                                                                                                                                               tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATc       >  1:1219107/1‑149 (MQ=255)
                                                                                                                                               tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGGCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATc       <  2:1094786/149‑1 (MQ=255)
                                                                                                                                                   gCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACGGACATTCACAGTGGACTGGAATCTTTg   <  1:1568677/149‑1 (MQ=255)
                                                                                                                                                   gCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGCCATTCACAGTGGACTGGAATCTTTg   >  1:116468/1‑149 (MQ=255)
                                                                                                                                                   gCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTg   >  1:703199/1‑149 (MQ=255)
                                                                                                                                                    ccTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGc  <  1:586167/149‑1 (MQ=255)
                                                                                                                                                    ccTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGc  >  2:1174846/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGC  >  NC_000913/2354170‑2354466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: