Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,399,842 C→A 5.7% E108D (GAG→GAT)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,399,8420CA 5.7% 242.0 / 2.5 99E108D (GAG→GAT) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base C (42/49);  new base A (3/3);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 108 total aligned reads displayed.

CACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGC  >  NC_000913/2399694‑2399989
                                                                                                                                                    |                                                                                                                                                   
cacaGCGGTAGCCGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGccc                                                                                                                                                     <  2:822258/149‑1 (MQ=255)
 acaGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCct                                                                                                                                                    >  1:1347282/1‑149 (MQ=255)
 acaGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCct                                                                                                                                                    >  1:1273532/1‑149 (MQ=255)
 acaGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCct                                                                                                                                                    >  1:774063/1‑149 (MQ=255)
       gTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAAAGGCACCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGTTCATCCCACTCAAAACCTTAAGATCGCAGTTACCGCCCTCTTcac                                                                                                                                              >  2:861087/1‑149 (MQ=255)
             ggCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCg                                                                                                                                        <  1:825511/149‑1 (MQ=255)
               cGATGCAGCGGTTCATTTCGTGAGATATGATTGGCCCCAAATCCTGATTACTGGGGGTTCGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGa                                                                                                                                      <  1:696404/149‑1 (MQ=255)
               cGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGCAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGa                                                                                                                                      <  2:945530/149‑1 (MQ=255)
                gATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGAc                                                                                                                                     <  1:599895/149‑1 (MQ=255)
                  tGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAg                                                                                                                                   <  1:537622/149‑1 (MQ=255)
                   gCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGt                                                                                                                                  >  1:657589/1‑149 (MQ=255)
                     aGCGGTTCATTTCGTGAGAGATGAATTGCCCCAAATCGTGATTACGGTGGGTACGTTTGGTGAAACGTTAGCGACGGCAGCTGTGTCCGGTGATCTCAGTCATCTCCTGAATATGGAAGTTACCGGCGTGTTCAAATACCGGACAGTct                                                                                                                                >  1:909961/1‑148 (MQ=255)
                     aGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCg                                                                                                                                >  1:756835/1‑149 (MQ=255)
                      gCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGt                                                                                                                               >  1:1403359/1‑149 (MQ=255)
                       cGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTg                                                                                                                              >  1:32016/1‑149 (MQ=255)
                        ggTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGc                                                                                                                             <  1:136830/149‑1 (MQ=255)
                        ggTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGc                                                                                                                             <  2:1151798/149‑1 (MQ=255)
                        ggTGCATTTCGTGAGCGATGAATGTCCCCAACTCCTGATTACGGGGGGTACGGTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGc                                                                                                                             <  2:1178124/149‑1 (MQ=255)
                        ggTGCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATAATGGTGGGGACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGc                                                                                                                             <  2:453941/149‑1 (MQ=255)
                           tCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGGCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCggg                                                                                                                          <  2:693980/149‑1 (MQ=255)
                              tttCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGggtgg                                                                                                                       >  1:770180/1‑149 (MQ=255)
                                tCGTGAGAGATGAATGGCCCCAAATCCTGATTACTGTGGGTACGTTTGGTGAACCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCTCCCTCTTCACATACCGGACAGTCGTGCGGGtggtt                                                                                                                     <  1:116382/149‑1 (MQ=255)
                                    gagagaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                                 >  1:439428/1‑149 (MQ=255)
                                    gacagctGAATGGCCCCAACTCCGGATTACGGGGGGGACGTTAGGGGACACGGTCTCGACGGAAGCTGTGTCCGGGCATCACAGTCATATCCTGAAGAGGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtc                                                                                                                 <  2:1028950/143‑1 (MQ=255)
                                        gaTGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAACCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGTAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGtcatca                                                                                                             <  1:1267479/149‑1 (MQ=255)
                                         aTGAATGGCCCCAAATCCTGATTACGGTGGGTAAGTTTGGTGAAAAGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCaa                                                                                                            <  1:296259/149‑1 (MQ=255)
                                          tGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGTCGGTAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAc                                                                                                           <  1:1289915/149‑1 (MQ=255)
                                          tGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAc                                                                                                           <  2:1273532/149‑1 (MQ=255)
                                          tGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAc                                                                                                           >  1:1235785/1‑149 (MQ=255)
                                           gAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATAACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAcc                                                                                                          >  1:1449165/1‑149 (MQ=255)
                                              tGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACt                                                                                                       >  2:1175472/1‑149 (MQ=255)
                                                 ccccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                    >  1:367924/1‑149 (MQ=255)
                                                 ccccAAATCCTGATTACGGTGGGTACGTTTGGGGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGa                                                                                                    <  2:1108631/149‑1 (MQ=255)
                                                  cccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAc                                                                                                   >  1:1191458/1‑149 (MQ=255)
                                                   ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAcc                                                                                                  <  1:759691/149‑1 (MQ=255)
                                                   ccAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGAcc                                                                                                  >  1:996042/1‑149 (MQ=255)
                                                          cTGATTACGGTGGGTACGTTTGGTGAAAAGGTAGCGACGGACGCTGTGTCCGGTCATCACAGTCATATCATGAAGATGGCAGTTACAGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAAACACTCGACCACGCttt                                                                                           >  2:537518/1‑149 (MQ=255)
                                                           tGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCttta                                                                                          >  1:1120542/1‑148 (MQ=255)
                                                            gATTACGGGGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGAGGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCa                                                                                         <  2:1030700/149‑1 (MQ=255)
                                                                  ggTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAAc                                                                                   >  2:577896/1‑149 (MQ=255)
                                                                  ggTGGGGACGTTTGGGGAACCGGTAGCGACGGAAGCTGTGTCCGGTCTTCAGAGTCATATCCTGAAGATGGCAGTTACCTCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCTCGCTTTCACGGAAc                                                                                   <  2:1429766/149‑1 (MQ=255)
                                                                       gaaCGTTTGGTGAATCGGTAGCGACGGAAGGTGGGTCCTGTCCTCACAGTCATTTCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCCGACAGGCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGttt                                                                              <  2:1203698/147‑1 (MQ=255)
                                                                       gTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGttt                                                                              <  2:46064/149‑1 (MQ=255)
                                                                             ttGGTGACACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGcttc                                                                        <  2:948964/149‑1 (MQ=255)
                                                                               ggTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGcttctt                                                                      >  1:1237973/1‑149 (MQ=255)
                                                                                gttaaaCGGTATCGACTGAAGCTGTGTCCGGTCATCACAGTCATAACCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGTAACTATTTCGCttcttc                                                                     <  2:189123/146‑1 (MQ=255)
                                                                                gTGAAACTGTAGCGTCGTAAGCTGTGTCCGGTAATGACAGTCATATCGTGAAGATGGCTGTTACCGCCCTCTTCACATACCGGTCATTCGTGCGGGTTGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                     <  1:1230260/149‑1 (MQ=255)
                                                                                gTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                     <  1:1175472/149‑1 (MQ=255)
                                                                                gTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                     <  2:1368635/149‑1 (MQ=255)
                                                                                gTGAAACGGTAGCCACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCatcttc                                                                     >  1:1162060/1‑149 (MQ=255)
                                                                                gTGAAACAGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCttcttc                                                                     <  2:209912/149‑1 (MQ=255)
                                                                                 tGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcg                                                                    >  2:58007/1‑149 (MQ=255)
                                                                                  gAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTtcgt                                                                   >  1:890033/1‑149 (MQ=255)
                                                                                     aCGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTcgtcgt                                                                <  1:1548913/149‑1 (MQ=255)
                                                                                     aCGGAAGCGACGGAAGCTTTGTCCGGTAATCACAGTCATATCCTTACGATGTCAGTTACCGCCCTCTTCACCTACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCTCGGAACTGTTTCGCTTCTTcgtcgt                                                                <  2:943485/149‑1 (MQ=255)
                                                                                          aGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATg                                                           <  2:756835/149‑1 (MQ=255)
                                                                                           gCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATgg                                                          >  2:1290979/1‑149 (MQ=255)
                                                                                            cGACGTAAGCTGTGTCCGTTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGa                                                         <  2:1036040/149‑1 (MQ=255)
                                                                                             gACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGaa                                                        <  2:185232/149‑1 (MQ=255)
                                                                                               ctgAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGTTGGAAGTTACAGCACTCTTCAGATTCCGGACAGTCGTGCGGGTGGTTGGTCATCAAATACTCGACCACGCTTTCACGTAACTGTTTCGCTTATTCGTCGTCAATGGAAAt                                                      <  2:1548763/147‑1 (MQ=255)
                                                                                                   aGCTGTGTCCGGTCATCACAGGAATATCCTGAAGATGGCAGATACCTCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTGCTTCGTCGTCAATGGAAATAAAg                                                  <  2:371657/149‑1 (MQ=255)
                                                                                                        tgtCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGcc                                             >  1:251273/1‑149 (MQ=255)
                                                                                                            cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCCCTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                         >  1:562610/1‑149 (MQ=255)
                                                                                                            cGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCg                                         >  1:1480413/1‑149 (MQ=255)
                                                                                                                 atcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGc                                    >  2:603403/1‑149 (MQ=255)
                                                                                                                  tcaCAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGcc                                   >  1:979324/1‑149 (MQ=255)
                                                                                                                    acaGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATAAACCACTCGACCACGCTTACACCGAACTGTTTCGCTTCATCGTCGTCAAAGCAAATAAAGGTGCCATCGGAAGCAgg                                 >  1:78503/1‑149 (MQ=255)
                                                                                                                     caGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                                <  1:203001/149‑1 (MQ=255)
                                                                                                                     caGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGGGGTTGGTCAGCAACCACGCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgt                                <  2:1403359/149‑1 (MQ=255)
                                                                                                                      aGTCATATCCTGACGATGGCCGTTACCGCCCTCTTCACATACCGGACAGTCGTGCTGGTGGTTGGTCATCAACCACTCGACCACTCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                               <  2:1549649/149‑1 (MQ=255)
                                                                                                                      aGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                               >  1:769698/1‑149 (MQ=255)
                                                                                                                      aGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtg                               >  1:618582/1‑149 (MQ=255)
                                                                                                                       gTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCCCGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGgtgt                              >  1:812076/1‑149 (MQ=255)
                                                                                                                          atatCCTGAAGATGGCAGTTACCGCCGTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                           >  1:495012/1‑149 (MQ=255)
                                                                                                                          atatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                           >  2:436866/1‑149 (MQ=255)
                                                                                                                          atatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCAt                           >  1:139182/1‑149 (MQ=255)
                                                                                                                           tatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTGATCAACCACTCGACCACGCATTCACGGAACTGTTTCGCTTGTTCGTCGTCAATTGAAATAAAGCTGCCATCGGAATCCGGTGTCATa                          >  2:702420/1‑149 (MQ=255)
                                                                                                                           tatCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGGGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTATTCGTCGTCACTGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                          <  1:861087/149‑1 (MQ=255)
                                                                                                                           tatCCTGAAGATGGCAGTTACCGCCATCTTCACATACCGGACAGTCGTGCGTGTGGTTGGTCATCAAACACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                          <  1:860598/149‑1 (MQ=255)
                                                                                                                           gtatgtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTCGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTGCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATa                          >  2:687861/6‑149 (MQ=255)
                                                                                                                            atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATAc                         <  1:1559059/149‑1 (MQ=255)
                                                                                                                            atCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTATTCTTCGGGAATGGAAAGAAAGGTGCCATCGGTAGCCGGTGTCAAAc                         >  2:1489310/1‑149 (MQ=255)
                                                                                                                              ccTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCGATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAg                       <  2:1347282/149‑1 (MQ=255)
                                                                                                                               gtGAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAgg                      >  1:1416206/2‑149 (MQ=255)
                                                                                                                                tGAAGATGGCAGTTACCGCCCTCTTCACATACCGCACAGTCGTGCGGGAGGTTGGTCATCACCCACTCGCCCACGCTGTCCCGGAACGGTTTGGCTTCTGCGTCGGCATTGGAAATAACGGTGCCACCTTCAGCGGGTGTCGTAGAGGa                     >  2:1447636/1‑149 (MQ=255)
                                                                                                                                 gAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAAAGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                    >  1:1212156/1‑149 (MQ=255)
                                                                                                                                 gAAGAGGGCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGAc                    >  1:1552433/1‑149 (MQ=255)
                                                                                                                                      gggCGGTAACTGCCATCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAc               <  2:1552433/148‑1 (MQ=255)
                                                                                                                                       ggCAGTTACCGCCCTCTTAACATACCTGACAGTAGTGCGGGTGGTTGGTCATCAACCACTCGACCACTCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCAcc              <  1:852947/149‑1 (MQ=255)
                                                                                                                                           ggtACCTCCCTCTTCACTTACCGGACAGTAGTGCGGGTGGTTGGTCATCAAACACTCGACCACGCTTTCACGGAACTGTTTCGCTTTTTCGTCGTCAATGGAAATAACGTTGCCATCGGAAGCAGGTGTCATAAAGGACATCACCAGGc          <  1:1224791/147‑1 (MQ=255)
                                                                                                                                           gTTACCGCCATCTTCACTTACCGGACAGGCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGc          <  2:78503/149‑1 (MQ=255)
                                                                                                                                             tACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGGCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGa        <  2:1191458/149‑1 (MQ=255)
                                                                                                                                              aCCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCAGCAACCACTCGCCCACGCTTTCACGGAACTCTTTAGCTTCTGCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCCGTGACATACAGGACATCACAAGCCgcc       >  2:411427/1‑147 (MQ=255)
                                                                                                                                                cGCCCTCTTCACATACCGGCCAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGTAACTAAAGGTTCCATCGGAAGCCGGTGTCATACAGGACATCCCCAGGCGACCa     >  2:1157655/1‑149 (MQ=255)
                                                                                                                                                cGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGCCCACGCTGTCCCGGAACTGTTTCGCTTGTTCGTCGACAATGGAAATAAAGGTGCCATCGGAATCCGGTGTCAGACAGGACTTCACCAGGCGAccc     >  1:388126/1‑148 (MQ=255)
                                                                                                                                                cGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGTCGGTGTCATACAGGACATCACCAGGCGACCa     >  1:1261835/1‑149 (MQ=255)
                                                                                                                                                  cccTCTTCCCATACCGGACAGTCGTGCGGGTGGTTGGTCAACAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTGCTTCGTCGTCAATGGAAATACAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcg   <  2:136539/149‑1 (MQ=255)
                                                                                                                                                  cccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcg   >  2:488131/1‑149 (MQ=255)
                                                                                                                                                  cccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCAcg   >  2:790141/1‑149 (MQ=255)
                                                                                                                                                   ccTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCTTATTTGATATCACCAGGCGACCAcgc  <  2:890033/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CACAGCGGTAGCAGGCGATGCAGCGGTTCATTTCGTGAGAGATGAATGGCCCCAAATCCTGATTACGGTGGGTACGTTTGGTGAAACGGTAGCGACGGAAGCTGTGTCCGGTCATCACAGTCATATCCTGAAGATGGCAGTTACCGCCCTCTTCACATACCGGACAGTCGTGCGGGTGGTTGGTCATCAACCACTCGACCACGCTTTCACGGAACTGTTTCGCTTCTTCGTCGTCAATGGAAATAAAGGTGCCATCGGAAGCCGGTGTCATACAGGACATCACCAGGCGACCACGC  >  NC_000913/2399694‑2399989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑