Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,462,677 A→T 5.5% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,6770AT 5.5% 376.3 / 5.8 134intergenic (+31/‑335)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (54/71);  new base T (5/3);  total (60/74)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

ACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTC  >  NC_000913/2462531‑2462825
                                                                                                                                                  |                                                                                                                                                    
aCGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCCTa                                                                                                                                                    >  2:117126/1‑149 (MQ=255)
  gTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAACGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCCTTCGCCTGGATAAAGTCACCTGCATAGc                                                                                                                                                  >  2:188034/1‑149 (MQ=255)
   ttGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCa                                                                                                                                                 <  1:1405455/149‑1 (MQ=255)
    tGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAg                                                                                                                                                <  2:788492/149‑1 (MQ=255)
     gttgtTTCTTATATGGACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGATCGCCTGGATAAAGGCACCTGCATAGCAgg                                                                                                                                               <  1:875388/147‑1 (MQ=255)
        gtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                            <  2:244549/149‑1 (MQ=255)
          ttCTTATATGCACGGTCAGAGCGAGAAAATTAACGAACGCCCATACCAGTTCGAGGCTGAAGGTAAAGCCTGGCTGTTCTGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACt                                                                                                                                          <  2:867884/149‑1 (MQ=255)
            cTTATAATCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAACGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACttt                                                                                                                                        <  1:454152/149‑1 (MQ=255)
             ttATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTa                                                                                                                                       >  1:1232033/1‑149 (MQ=255)
               atatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAAc                                                                                                                                     >  2:456027/1‑149 (MQ=255)
                  tGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATACCGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTcc                                                                                                                                  <  1:627077/149‑1 (MQ=255)
                  agCACGGTCAGAGCGTGAAAATTAACGAAGGACCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGATGTACGGTACAAAATTTAACAACGCGTTATGATAACGCTTTCGCATGGATAAATTCACCTGCTATGCTGGTTACTTAAACTcc                                                                                                                                  >  1:1099307/2‑149 (MQ=255)
                   gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGCCTTTAACTcc                                                                                                                                  >  2:876087/1‑148 (MQ=255)
                   gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                 >  2:1247750/1‑149 (MQ=255)
                   gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                 >  1:104359/1‑149 (MQ=255)
                   gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTcc                                                                                                                                  <  1:876087/148‑1 (MQ=255)
                    cACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAATGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTaccc                                                                                                                                >  2:667627/1‑149 (MQ=255)
                       gggcAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCAc                                                                                                                             <  2:1107894/146‑1 (MQ=255)
                       ggTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCAc                                                                                                                             <  2:943311/149‑1 (MQ=255)
                         tCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACtt                                                                                                                           >  1:545598/1‑149 (MQ=255)
                           agagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGCCTTTAACTCCCCCACTTCa                                                                                                                         >  2:953091/1‑149 (MQ=255)
                           agagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGAAAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCa                                                                                                                         <  1:1296241/149‑1 (MQ=255)
                             cgCGTGATAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTGCTTATAACTCGTTCGCCTGGATAATGTCACCTGCTTAGCAGGTGACTTTAACTCCCCCACTTCAcc                                                                                                                       <  2:1098822/148‑1 (MQ=255)
                             agCGTGAATATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGTTAAAGTCACCTGCATAGCAGGTGACTTTAGCTCCCCCACTTCGcc                                                                                                                       >  1:731869/1‑149 (MQ=255)
                             agCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAcc                                                                                                                       >  1:211449/1‑149 (MQ=255)
                              gCGAGAAAATTAACGATGGCCCATACCAGTGCGAGTCTGAAGGTTATGCCTGGCTGTTCTGTTCTCTCTTTAACTTCGCGTTCTGATACCGCGTTCGCCTGGATAAAGTCACCTGATTAGAAGGTGAGTTGATATCCCCCACTTCAcag                                                                                                                      >  2:1534059/1‑147 (MQ=255)
                                 tGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                   <  1:1178037/149‑1 (MQ=255)
                                  gAAAATTCACGAAGGCCCATACCAGTTCGAGTCTGTAGGTAAAGCCTGGCTGTTCGGTACTAACTGAAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAag                                                                                                                  <  2:321283/149‑1 (MQ=255)
                                   aaaaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTACACCGAAagt                                                                                                                 >  2:414349/1‑149 (MQ=255)
                                     aaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtag                                                                                                               >  2:1567276/1‑149 (MQ=255)
                                     aaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtag                                                                                                               >  2:1412223/1‑149 (MQ=255)
                                        tAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATATCGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAgtgt                                                                                                            >  1:1073048/1‑149 (MQ=255)
                                          aCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAATTAGTGTcc                                                                                                          >  2:633614/1‑149 (MQ=255)
                                           cGAAGGCCCATACCAGTTCGAGTCTGATGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGATCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTGCCCCACTTCACCGAAAGTAGTGTCCt                                                                                                         >  1:283392/1‑149 (MQ=255)
                                            gAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCta                                                                                                        >  2:1196692/1‑148 (MQ=255)
                                                  ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                  <  2:1282822/149‑1 (MQ=255)
                                                  ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                  >  2:778815/1‑149 (MQ=255)
                                                   cATACCAGTTCGAGTCTGAAGGTACAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                 <  2:626670/149‑1 (MQ=255)
                                                   cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                 <  1:400151/149‑1 (MQ=255)
                                                    aTACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTa                                                                                                >  2:1482991/1‑149 (MQ=255)
                                                     tACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAc                                                                                               <  1:1432106/149‑1 (MQ=255)
                                                       ccAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCATGTGACTTTAACTCCCCCACTTCACCGAAAGTATTGTACTCATTGCGTAcat                                                                                             >  1:1540832/1‑147 (MQ=255)
                                                             cGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAg                                                                                       >  1:1220039/1‑149 (MQ=255)
                                                              gAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGt                                                                                      >  2:709931/1‑149 (MQ=255)
                                                                 tcagAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGAGGTCTGATAACGCGATCGCCAGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGtttt                                                                                   <  2:572975/146‑1 (MQ=255)
                                                                 tCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTACCTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGtttt                                                                                   >  2:160232/1‑149 (MQ=255)
                                                                  cTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTTATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTg                                                                                  <  2:1305315/149‑1 (MQ=255)
                                                                   tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGc                                                                                 >  1:26825/1‑149 (MQ=255)
                                                                    gAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCa                                                                                <  1:1181167/149‑1 (MQ=255)
                                                                    gAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCa                                                                                >  2:244834/1‑149 (MQ=255)
                                                                      aGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCaaa                                                                              <  1:977357/149‑1 (MQ=255)
                                                                          aaaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGc                                                                          >  2:659031/1‑149 (MQ=255)
                                                                           aaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGcc                                                                         >  2:901061/1‑149 (MQ=255)
                                                                              ccTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTTCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGt                                                                      <  2:1181060/149‑1 (MQ=255)
                                                                              ccTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGt                                                                      >  1:171772/1‑149 (MQ=255)
                                                                               cTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                     <  2:568024/149‑1 (MQ=255)
                                                                               cTGGCTGTTCGGCACTAACTTTAACTACGCGTGCTGATAACGCGTTCGCCTGTATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                     <  1:706375/149‑1 (MQ=255)
                                                                                tGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTg                                                                    <  2:58350/149‑1 (MQ=255)
                                                                                 ggCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTgg                                                                   <  1:124289/149‑1 (MQ=255)
                                                                                    tGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCACGTGACTATAACTCCCCCACTTCACCGAAAGTATTGTCCTCATTGCTTACATCCTGAGTTTTGCAAAAAGCCTGTTGGCAg                                                                >  1:1080497/1‑149 (MQ=255)
                                                                                     gTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCAGTTGGCAGc                                                               >  1:924237/1‑149 (MQ=255)
                                                                                      ttCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGGGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGACTTTTTCAAACAGCCTGTTGGCAGCt                                                              >  1:261137/1‑149 (MQ=255)
                                                                                          gTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCCCCGTAAGTAGTGTCCTCATAGCTTACCTCCTGAGTTTTGCAAACTGCCTGTTGGCCGCATGcc                                                          >  2:1377709/1‑149 (MQ=255)
                                                                                              tAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGGTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                      >  2:1487629/1‑149 (MQ=255)
                                                                                              tAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                      >  2:595738/1‑149 (MQ=255)
                                                                                              tAACTTTAACTACGCGGTCTGATAACGCGTTCGCCTGTATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                      <  2:104042/149‑1 (MQ=255)
                                                                                               aaCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTACCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCATACAGCCTGTTGGCAGCTTGCCCTTTc                                                     >  1:794346/1‑149 (MQ=255)
                                                                                                   ttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAAt                                                 <  1:1069168/149‑1 (MQ=255)
                                                                                                   ttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAAt                                                 <  1:1322536/149‑1 (MQ=255)
                                                                                                   ttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAAt                                                 <  2:223017/149‑1 (MQ=255)
                                                                                                    tAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATc                                                <  2:1173110/149‑1 (MQ=255)
                                                                                                      actactGGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGTTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCTAACAGCCTTTTGGCAGCTTGCCGTTTCAAATCaa                                              <  2:1099307/149‑1 (MQ=255)
                                                                                                          cgcgTTCTGATAACTCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAACTAAAGTAGTGTCCTCATTGCTTAGCTCCTGACTTTTGCGCTCAGCCTGTTGGCCGCTTGCCCTTTCAAATCAATAAg                                          >  1:321683/1‑149 (MQ=255)
                                                                                                                    tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                <  2:781816/149‑1 (MQ=255)
                                                                                                                    tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                <  1:907944/149‑1 (MQ=255)
                                                                                                                    tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                <  1:414349/149‑1 (MQ=255)
                                                                                                                     aaCGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcaca                               >  1:1226082/1‑149 (MQ=255)
                                                                                                                       cgcgTTCGCCTGGATAAAGTCACATGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGa                             <  1:1476273/149‑1 (MQ=255)
                                                                                                                        gcgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCCCCGAAAGTAGTGTCCTCATTTCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTGTCAAATCAATAAGCGGTATCCACAgcc                            >  2:1375621/1‑147 (MQ=255)
                                                                                                                        gcgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGAc                            <  1:1482991/149‑1 (MQ=255)
                                                                                                                         cgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGTCTTTAACACCCCCCCATCACCGCACGTAGTGTCCTCATTGCTTACCTCCTGAGGTTTGCCAACAGCCTTTTGGCTGCTTGCCCTGACAAAGAAAATGGCGGGAGCCACAGACa                           >  1:992804/1‑149 (MQ=255)
                                                                                                                           ttCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTTGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACaaa                         <  1:1197435/149‑1 (MQ=255)
                                                                                                                            tCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAAc                        <  2:1319774/149‑1 (MQ=255)
                                                                                                                            tCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAAc                        <  1:1412223/149‑1 (MQ=255)
                                                                                                                            tCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAAc                        <  1:1567276/149‑1 (MQ=255)
                                                                                                                             cGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAAcc                       <  1:1096591/149‑1 (MQ=255)
                                                                                                                                       aaGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGaaa             <  2:545598/149‑1 (MQ=255)
                                                                                                                                        aGTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTACTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg            >  1:451874/1‑149 (MQ=255)
                                                                                                                                        aGTCACCTGCATAGCATGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg            <  1:188034/149‑1 (MQ=255)
                                                                                                                                        aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAATGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGAATCCACAGACAAACCTGAAGGGAAAg            >  1:1297817/1‑149 (MQ=255)
                                                                                                                                        aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg            <  1:456027/149‑1 (MQ=255)
                                                                                                                                        aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACATGAAGGGAAAg            >  1:709828/1‑149 (MQ=255)
                                                                                                                                          tCACCTTCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCATGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGAGAAACCTGAAGGGAAAGGc          <  1:42647/149‑1 (MQ=255)
                                                                                                                                          tCACCTGCTATGCAGGTGACTTTTACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGTTATCCACAGACAAACCTGAAGGGAAAGGc          >  2:345417/1‑149 (MQ=255)
                                                                                                                                          tCACCTGCTATGCAGGTGACTTTACCTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAggg          >  1:737563/1‑148 (MQ=255)
                                                                                                                                           cACCTGCATAGCAGGGGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCa         <  2:104359/149‑1 (MQ=255)
                                                                                                                                            aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGCAAGTACTGTCCTCAGTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGGAGCTAGCCCTTTCAAAACACTAATCGGTATCCACAGACAAACCTGCAGGGATAGGCat        >  1:87258/1‑149 (MQ=255)
                                                                                                                                            aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCat        <  1:709931/149‑1 (MQ=255)
                                                                                                                                              cTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAAAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCAAAGACAAAACTGAAGGTAAAGGCatat      >  1:1176101/1‑149 (MQ=255)
                                                                                                                                                 cATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATAtttt   <  1:1196692/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTC  >  NC_000913/2462531‑2462825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: