Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,904,212 G→A intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,904,2120GA95.2% 249.8 / NA 105intergenic (+838/+535)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (49/49);  minor base C (4/1);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCC  >  NC_000913/2904067‑2904354
                                                                                                                                                 |                                                                                                                                              
cAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGacgc                                                                                                                                             <  1:572541/149‑1 (MQ=255)
  ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgg                                                                                                                                           <  1:497063/149‑1 (MQ=255)
  ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCgg                                                                                                                                           <  2:1109131/149‑1 (MQ=255)
   gCTCCGGTTTATCCCCGCTGGCGGGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggg                                                                                                                                          <  1:319556/149‑1 (MQ=255)
   gCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTCTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggg                                                                                                                                          <  1:839510/149‑1 (MQ=255)
     tCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGa                                                                                                                                        >  2:1150536/1‑149 (MQ=255)
     tCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTATTCGCAAATCTATGGACTAATGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACCCCGGCGCACGGAATACAAAGCCATGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGa                                                                                                                                        >  2:613058/1‑149 (MQ=255)
      ccGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggggga                                                                                                                                       >  1:353887/1‑147 (MQ=255)
         gtgtagacccGCTGGCGCGGGGAACACCGCCCTACGCAAATCTATGGACAATTGCTATTCGCTTTAGCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac                                                                                                                                    <  1:736184/142‑1 (MQ=255)
         gTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac                                                                                                                                    <  1:496395/149‑1 (MQ=255)
            tATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTgg                                                                                                                                 >  1:908825/1‑149 (MQ=255)
            tATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTgg                                                                                                                                 <  2:745724/149‑1 (MQ=255)
            aaTCCCCTCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGTAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTgg                                                                                                                                 <  1:1178103/148‑1 (MQ=255)
                cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACAATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                             <  1:732558/149‑1 (MQ=255)
                 ccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACCCTGGCTCTg                                                                                                                            >  1:142471/1‑149 (MQ=255)
                    cTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCaa                                                                                                                         <  2:312124/149‑1 (MQ=255)
                     tGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAc                                                                                                                        <  1:389537/149‑1 (MQ=255)
                      ggCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAca                                                                                                                       <  2:349777/149‑1 (MQ=255)
                       gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAAATATTAGCCGTGTATGTGATCGGTTTATCCACGCTGGCGCGGGGAAAGCTGGCTCTGGAAcag                                                                                                                      >  1:1248655/1‑149 (MQ=255)
                         gcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                    <  2:169134/149‑1 (MQ=255)
                           ggggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACagcagc                                                                                                                  <  1:633976/149‑1 (MQ=255)
                            gggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGTAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAgcagca                                                                                                                 <  2:87398/149‑1 (MQ=255)
                             ggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAc                                                                                                                <  1:774821/149‑1 (MQ=255)
                                  acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGACTCTGCAACAGCAGCACACATg                                                                                                           >  1:1073729/1‑149 (MQ=255)
                                  acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAATGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAAAACTGGCTCTGCAAAGGCAGAACCCaag                                                                                                           >  2:896213/1‑147 (MQ=255)
                                    cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGAc                                                                                                         <  1:516636/149‑1 (MQ=255)
                                    cTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCAGTGTATGTGCTCGGTTTATCCCCGCTGACCCGGGGAGCACTGGCTCTGCAACGGCAGCACCCATgcc                                                                                                         >  1:840716/1‑147 (MQ=255)
                                       ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCCCGGAATACTAAGCCGTGAATCTTCTAGGTTTATCCCCGCTGACGCGGGGAACACTGGCTATGCAACAGAACCAGCCATGACCAc                                                                                                      >  2:720397/1‑149 (MQ=255)
                                       ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCAc                                                                                                      <  2:463996/149‑1 (MQ=255)
                                       ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAAGACAAAGCCGTGTATCTGATAGGTTTATCCCCGCTGCCGCGGGGAACACAGGCTATGACACCGCAGCACCCATgcacac                                                                                                      >  2:720400/1‑144 (MQ=255)
                                          tCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTc                                                                                                   <  2:17193/149‑1 (MQ=255)
                                           cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCg                                                                                                  <  1:707779/149‑1 (MQ=255)
                                            gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACATAGCCGAGTATGTGCTCGGTTTATCCCCGCTGACGCGGGGACCACTGGCTCTGCAACAGCCGCACCCAGGACCACGTCgg                                                                                                 >  2:447140/1‑149 (MQ=255)
                                            gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCgg                                                                                                 >  2:657851/1‑149 (MQ=255)
                                            gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACAGGCAATACAAAAGCAGCACCCATGAACACTTCgg                                                                                                 >  2:657835/1‑149 (MQ=255)
                                                 tCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTAt                                                                                            >  1:670094/1‑149 (MQ=255)
                                                    aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGTAGCCCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGGACCCATGACCACGTCGTTTTATccc                                                                                         >  1:814711/1‑149 (MQ=255)
                                                     tGGACTATTTCTATTCGGTTTATCCCCGCTTGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATcccc                                                                                        <  1:755162/149‑1 (MQ=255)
                                                     tGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAAGACCACGTCGGTTTATcccc                                                                                        >  1:1136592/1‑149 (MQ=255)
                                                      ggACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATccccc                                                                                       >  1:388641/1‑148 (MQ=255)
                                                       gtcttttGGTATTCGGTTTATCCCCGCTGGCGCGGGGTACAAGGGCGCACGGAATACAAAGCCGTGTATCTGGTCGGTTTATCCCCGCTGACGCGGGGAACTCTGGCTCTGCTACAGCTGCACCCATGAGCAGGTCGGTTTATCCCCGc                                                                                      <  2:1287423/144‑1 (MQ=255)
                                                       gACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGGGTATCTGGTCGGTTTATCCCCGCTGACGCGGGGAACCCTGGCTCTGCAACAGCAGCACACATGACGAGGTCGGTTTATCCgcgc                                                                                      >  2:1296667/1‑149 (MQ=255)
                                                        aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCt                                                                                     >  2:123680/1‑149 (MQ=255)
                                                            ttGCTATTCTGTTTATCTCCGCTGGAGCGGGGATCACGCGAGCACTTAATACTATGCCGTGTATCTGCTGGGTTTATACCCGCTGTCGCGGGGAACACTGGCTCTGCTACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGgcg                                                                                 <  1:89208/149‑1 (MQ=255)
                                                            ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCAAGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACAGGCTCTGCAACAGCCGCACCCATGACGAAGTCGGTTTATGCCCGCGGgcg                                                                                 >  2:424668/1‑149 (MQ=255)
                                                              gCTATTCGGTTTATCGCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTTCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCAGCCATGACCACGTCGGTTTATCCCCGCTGGcgcg                                                                               <  2:676449/149‑1 (MQ=255)
                                                                  ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACGACGTCGGTTTATCCCCGCTGGCGCGGGGa                                                                           >  1:777536/1‑149 (MQ=255)
                                                                   tCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGATTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGGCGGTTTATCCCCGCTGGCGCGGGGaa                                                                          <  2:874982/149‑1 (MQ=255)
                                                                   ccGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATCCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                          <  1:158628/148‑1 (MQ=255)
                                                                    cGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAac                                                                         <  1:516600/149‑1 (MQ=255)
                                                                      gTTTATCAGCGCTGGTGCTGTGAACACGGGCGCACGGATTACAAAGCCGTGTATCTGCGCGTTTTATCCCCGCTGACCGGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAacac                                                                       <  2:1291744/149‑1 (MQ=255)
                                                                        ttctCCCCGCTGGCGCGGGGAACACGGGCGCACGGACTACAACGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCACCAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGa                                                                     <  1:731342/146‑1 (MQ=255)
                                                                        ttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGa                                                                                                          >  1:262686/1‑112 (MQ=255)
                                                                        ttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGa                                                                                                          <  2:262686/112‑1 (MQ=255)
                                                                        ttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGa                                                                     <  1:386798/149‑1 (MQ=255)
                                                                             cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACCCTGGCTCTGCAACAGCAGCACCCATGACCACGGCGGTTTATCCCCGCGGGCGCGCGGAACACGAAAAGc                                                                >  1:233157/1‑149 (MQ=255)
                                                                             cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCAAACGAAATgg                                                                >  1:490359/1‑148 (MQ=255)
                                                                              ccGCTGGCGCGGGGAACACGGGCGAACGGAATACAAAGACGTGTATCTGCTCGGTTTATCCCCGCTGACGAGGGGAAAACTGGCTATGCAACAGAAGCACCCATGACCAAGTGGGTTTATACCCGCTGGCGCGGGGAAGACGactggcg                                                               >  2:781236/1‑143 (MQ=255)
                                                                               cGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                              <  1:1080877/149‑1 (MQ=255)
                                                                                 cTGGCGGGGGGAACACGGGCGCACGGAATACTAAGCCGTGTATCTGCTCGGTTTATCGCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGTCCTCGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGt                                                            <  1:294809/149‑1 (MQ=255)
                                                                                 cTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCAGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGt                                                            <  1:1034068/149‑1 (MQ=255)
                                                                                     cgcgTGGAACACGGTCGCTCGGAATACAAAGCAGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCGATGACCTCGTCGGTTTATCCGCGCTGGCGCGGGGAACACGAAATGCTGGTGAGc                                                        <  1:1296667/149‑1 (MQ=255)
                                                                                       cgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGt                                                      <  1:1187780/149‑1 (MQ=255)
                                                                                       cgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGt                                                      <  1:663373/149‑1 (MQ=255)
                                                                                       cgGGGAACACGGGCGCACGGAAGACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGt                                                      <  2:548765/149‑1 (MQ=255)
                                                                                         gggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTa                                                    >  2:505734/1‑149 (MQ=255)
                                                                                            aaCACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATg                                                 >  1:426868/1‑149 (MQ=255)
                                                                                               acGGGCGCCCGGAATCCAACGCCGTGGATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCg                                              <  1:845647/149‑1 (MQ=255)
                                                                                               acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGTGGAACACGAAAAGCTGGTTCGCGTTAATGCCg                                              >  2:767035/1‑149 (MQ=255)
                                                                                               acGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTAAATTCCg                                              >  1:715059/1‑149 (MQ=255)
                                                                                                cGGGCGCACGGAATACCAAGCCTTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCTGAACCCATGACGACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGc                                             <  2:369649/149‑1 (MQ=255)
                                                                                                   gcgcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCaaa                                          >  1:179991/1‑149 (MQ=255)
                                                                                                     gcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAaca                                        >  1:1084064/1‑149 (MQ=255)
                                                                                                     gcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAaca                                        <  1:632579/149‑1 (MQ=255)
                                                                                                      cACGGAATACAAAGCCGTGTATCTGCGCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAacac                                       <  1:79147/149‑1 (MQ=255)
                                                                                                       aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCCGGGAACACTGGCTCTGCAACAGCAGCAACCATGACCACGTGCGTTTATCCCCCCTGGCGCGGGGAACACGAAATGATGGTCAGCGTTAATGCCGCAAAaaca                                      >  1:781143/1‑149 (MQ=255)
                                                                                                        cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                     <  1:657851/149‑1 (MQ=255)
                                                                                                         ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                    >  1:978228/1‑149 (MQ=255)
                                                                                                           aaTACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGtt                                  >  1:1118261/1‑149 (MQ=255)
                                                                                                                aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcc                             >  2:318170/1‑149 (MQ=255)
                                                                                                                aaaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAAAACGAAATGCTGTTGAGCGTTAATGCCGCAAACACAGGTTTATcc                             >  2:167488/1‑149 (MQ=255)
                                                                                                                aaaGCCGTGTATCAGCTCGGTTTATCCCCGCTGACGCGTGGAACACAGGCTCTGCAACAGCCGCACCCATTACCACGTCGGTTTATCACCGCAGGCGCGGTGAACACGAAATGCTGGTAGGCGGTAATGCCGCAAACACAGGTTTATcc                             >  1:881987/1‑149 (MQ=255)
                                                                                                                 aaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATccc                            >  1:653568/1‑149 (MQ=255)
                                                                                                                    ccGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTATTGCCGCAATCACAGGTTTATCCCCGc                         >  1:471426/1‑149 (MQ=255)
                                                                                                                    ccGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGc                         >  2:311430/1‑149 (MQ=255)
                                                                                                                         tATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgcg                    >  1:490345/1‑149 (MQ=255)
                                                                                                                          aTCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGGCCAGGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGgcgc                   >  1:327176/1‑149 (MQ=255)
                                                                                                                            cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCgg                 <  1:97312/149‑1 (MQ=255)
                                                                                                                             tGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                >  1:559424/1‑149 (MQ=255)
                                                                                                                             tGCCCGGTTTATCCCCGCTGCCGCGGGGAACACTGGCGCTGCAACAGCAGCACCCATGCCCACCGCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                <  1:335963/149‑1 (MQ=255)
                                                                                                                              gCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGATGGCGCgggg               >  2:304989/1‑149 (MQ=255)
                                                                                                                                tCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGac             >  1:990994/1‑148 (MQ=255)
                                                                                                                                tCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa             >  2:793903/1‑149 (MQ=255)
                                                                                                                                tCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaa             >  2:1279203/1‑149 (MQ=255)
                                                                                                                                  ggTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGGACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGaagc           >  1:918581/1‑147 (MQ=255)
                                                                                                                                  ggTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCGGGTGAGCGGTAAGGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGcaca           >  1:861859/1‑149 (MQ=255)
                                                                                                                                    tttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGCCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAACAGCGGGTGCGCGTTAATGCCGCACACCCCGGTTTATCCCCGCTGGCGCGGGGAacacc         >  1:270707/1‑148 (MQ=255)
                                                                                                                                    tttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCTGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAAcaca         <  2:142471/149‑1 (MQ=255)
                                                                                                                                        accccGCTGACTGGGGGAACACTGGCTCTGCCACAGCCGCTCCCATGACCACGTCGGGTTATCCCTGCTGGCGCGGGGAAGACGAAAGGCTGGTGAGCGTTTTTGCCGCATACTCAGGTTTTTCCCCGCGGGCGCGGGGAACACATTAc     <  2:1181633/148‑1 (MQ=255)
                                                                                                                                           ccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcc  >  2:948464/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCC  >  NC_000913/2904067‑2904354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: