Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 10.0% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA10.0% 393.3 / 27.6 158noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (81/56);  new base A (7/8);  total (94/64)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

TCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287002‑1287298
                                                                                                                                                   |                                                                                                                                                     
tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCGGGCGCTCGAg                                                                                                                                                     >  1:3914358/1‑150 (MQ=255)
  cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                   >  2:1567432/1‑150 (MQ=255)
  cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                   >  2:2971189/1‑150 (MQ=255)
  cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                   >  1:3627635/1‑150 (MQ=255)
    cGCATGGGCAGAATAGTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGa                                                                                                                                                 <  1:3202204/150‑1 (MQ=255)
     gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGGCTGGGGCAAAGCTATATAGCTGGCGCTGGAGTGGaa                                                                                                                                                >  2:1691176/1‑150 (MQ=14)
         gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCtcgaatcgaaacat                                                                                                                                            >  1:2807243/1‑146 (MQ=255)
           gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTgg                                                                                                                                          >  2:930485/1‑150 (MQ=255)
             aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCTCATCTCCCTTCGGGTCGTTGCCTGAGGCAACGATCTCTCGCTTTCGCTAGATACGAAcatagggc                                                                                                                                        >  2:3192745/1‑143 (MQ=14)
                   tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTAGAAGCt                                                                                                                                  >  2:2857058/1‑150 (MQ=255)
                    ttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCATGGTCGAAGCtt                                                                                                                                 >  1:2901855/1‑150 (MQ=255)
                     tGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                                <  1:1641128/150‑1 (MQ=255)
                        ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTTCGTGTCGTTTCCTGCGGCAAAGCTCTATCGCTGTCGCAAGAGTCTAACCTTGGTCGAAGATTCTCa                                                                                                                             >  2:2807542/1‑150 (MQ=14)
                           cGGATTCGCTTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                                          <  2:2968803/150‑1 (MQ=255)
                           cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCGCGGTGGCGCGCGAGTGGAACCGTGGGGGCAGGTTCTCaaac                                                                                                                          >  1:2269855/1‑147 (MQ=14)
                                  gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                   >  2:1964697/1‑150 (MQ=255)
                                  gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCTCGGGCGCTCGAGTCGAACCATGGTCGAAGCTTCTCATGCTGCCCCg                                                                                                                   >  2:3410970/1‑150 (MQ=14)
                                   cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCAGGCGCGCGAGTCGAACCATGGTCGAAGCTTCTCATCCATACCCGc                                                                                                                  >  1:1908831/1‑150 (MQ=14)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTATTCTTCGCTGCGCTCATCTCCCTTCGGGACGTTGCCTGGGGCAACGCTCTCTCGCTTTCGCTCGAATCGAACCTTGGTCGAAGCTTCTAAACCTTACCCGcg                                                                                                                 >  1:171859/1‑149 (MQ=14)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACGCACACTAGCTGGCCCAACAGTAGAAACTTGGTCGAATCATCACAACCATCCCCCCa                                                                                                                 >  2:417919/1‑150 (MQ=14)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                                 >  2:2630297/1‑150 (MQ=255)
                                    ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATACTTCCCCGCa                                                                                                                 >  2:436960/1‑150 (MQ=255)
                                     tGAGAGTTCAGTGACTTTTGTAAGTGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                <  1:3195928/150‑1 (MQ=255)
                                       agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATgg                                                                                                              >  2:3031327/1‑150 (MQ=255)
                                          gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTTGTCGAAGCTTAACAACCTTACCCCCATGGGcc                                                                                                           >  1:2184613/1‑149 (MQ=14)
                                           ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                                                          <  1:1760725/150‑1 (MQ=255)
                                              aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                       >  2:641540/1‑150 (MQ=255)
                                              aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCTACCCCGCAAGGGCAGaaa                                                                                                       >  2:515618/1‑149 (MQ=255)
                                              aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAat                                                                                                       >  2:515906/1‑150 (MQ=255)
                                               gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCTCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACGCTCTCTCGCATTCGCACCAATCGAACCTTGGTCCAAGCTTCTAAAACATAACCCCATGGGCAGActa                                                                                                      >  2:1724859/1‑150 (MQ=11)
                                               gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAata                                                                                                      <  1:1649639/150‑1 (MQ=39)
                                                ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCAAGCGGGCGCACGAGGAGAACCATGGTCGAAGCATCAAATCCATACCCCCCAGGGCAGacaat                                                                                                     >  1:1594031/1‑146 (MQ=11)
                                                    ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCAACCCACACACGCAGGCGCTCGAGGCGAAACATGGTGCAAGCTTCTCAAACTTCCCCCCAGGGGCAGGCTAtttga                                                                                                 >  1:1522769/1‑148 (MQ=11)
                                                    ctttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTTCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 >  2:3401566/2‑150 (MQ=255)
                                                        gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  2:3841498/150‑1 (MQ=255)
                                                        gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACTCACGCTGGCGCACGACTCGAACCGTGGTCGAAGCTTATCAAACATCCCCCCAAGGGCAGAAAAATTaaattc                                                                                             >  2:2999333/1‑146 (MQ=11)
                                                            gTGATGGTGGTGAGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAt                                                                                         <  1:1157416/150‑1 (MQ=38)
                                                                 ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    <  1:300044/150‑1 (MQ=37)
                                                                       gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGGCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                              >  2:313198/1‑150 (MQ=255)
                                                                           gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          <  1:1008130/150‑1 (MQ=255)
                                                                            aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGGTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  2:3262570/150‑1 (MQ=255)
                                                                                  tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                   <  1:3509463/150‑1 (MQ=255)
                                                                                   ccTCACCGCCGCGCGCGTCGCCCTTCGGCCCGTGGCCTGCGCCAACGACATCTCTCTGTCGCTCGTGTCGCTCCTTGCTCGAATCTCCACATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                  <  2:2471288/149‑1 (MQ=9)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                             >  2:877749/1‑105 (MQ=35)
                                                                                     tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                             <  1:877749/105‑1 (MQ=35)
                                                                                      cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTc                                                                                                                       <  1:2834949/94‑1 (MQ=33)
                                                                                      cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTc                                                                                                                       >  2:2834949/1‑94 (MQ=33)
                                                                                       ctcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                              <  1:3657706/148‑1 (MQ=255)
                                                                                              cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGGCGAAAATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAgggttg                                                       >  2:3542313/1‑145 (MQ=17)
                                                                                                   ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                              <  1:3284518/90‑1 (MQ=38)
                                                                                                   ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                              >  2:3284518/1‑90 (MQ=38)
                                                                                                     cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                                <  2:3026846/150‑1 (MQ=255)
                                                                                                       cccTTCGGGTCGTTGCCAGCGGCAACGCTCCCCCGCTGGCGCTCGAGCCGAACCTTTGTCGAAGGTTCTCATCCTTCCCCACATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                              <  2:1655123/150‑1 (MQ=14)
                                                                                                          ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:2707195/150‑1 (MQ=255)
                                                                                                          ttCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCCCAAGGGCAGAATATTTAAATTCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGaa                                           >  2:1340674/1‑150 (MQ=17)
                                                                                                           tCGGGTCGTTGCCTGCGGCAACGCTCTGTGTCTGTCGCTCGAGTCGTAGCTTTGTCGAATCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          <  1:2406286/150‑1 (MQ=14)
                                                                                                            gagagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                         >  1:3481876/6‑150 (MQ=255)
                                                                                                             agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGACGGa                                        >  2:2862256/5‑150 (MQ=14)
                                                                                                              gagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                       >  1:3854625/4‑150 (MQ=17)
                                                                                                                    tGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:3633198/150‑1 (MQ=255)
                                                                                                                      ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:2194973/1‑150 (MQ=255)
                                                                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGa                                                                                          <  2:1997343/90‑1 (MQ=18)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                                <  1:1779812/51‑1 (MQ=35)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGCTTAGTTGGGAAGTTCAGGGACTTTTGAAAGTGATTGTGGTGGGGGTAGGATTCGAACCTTc                             >  1:2434472/1‑150 (MQ=17)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  2:1883908/150‑1 (MQ=255)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGGAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGGAGGGTTCGAACCTTc                             >  1:881158/1‑150 (MQ=17)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                            >  1:100878/1‑87 (MQ=18)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                            <  2:100878/87‑1 (MQ=18)
                                                                                                                        tGCGGCAACGCTCTCTCGCTGGCGATCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:3410970/150‑1 (MQ=255)
                                                                                                                        gcaggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:1976672/147‑1 (MQ=255)
                                                                                                                           ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGACGTTTAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          >  2:1347611/1‑150 (MQ=255)
                                                                                                                           ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                          <  1:2776950/150‑1 (MQ=255)
                                                                                                                              aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATAATACTTACCCGAATTGGCAGAATATTTAATATCGGATTAGTTGGGAAGTTAAGGGACTTTTTAAAGGGAAGGGGGTTGGGGAAGGATTCGAAAATTCGaatta                       >  1:2543963/1‑147 (MQ=17)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          <  1:1538604/94‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  <  1:2150245/150‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATTGGCAGAATATTTAAATTCGGATTCGTTGGGAAGATAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACATTAGAAGAAGATGa                  >  1:951096/1‑150 (MQ=17)
                                                                                                                                   tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCa                                                                          >  2:2027151/1‑94 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          <  1:2027151/94‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          >  1:912956/1‑94 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          <  2:912954/94‑1 (MQ=255)
                                                                                                                                   tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          >  2:1538604/1‑94 (MQ=255)
                                                                                                                                      ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:3517260/1‑147 (MQ=255)
                                                                                                                                      ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACATTCGAAGTCGATGACgg               >  2:460326/1‑150 (MQ=255)
                                                                                                                                       tcGCCGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATTTTTTATTGCGGATTCGTTGGGCAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc              <  1:1397940/150‑1 (MQ=18)
                                                                                                                                        cGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGTCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa             <  1:2191125/150‑1 (MQ=255)
                                                                                                                                        cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa             <  1:515906/150‑1 (MQ=255)
                                                                                                                                        cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa             <  1:515618/150‑1 (MQ=255)
                                                                                                                                         gCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         >  2:1102369/1‑105 (MQ=255)
                                                                                                                                           tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          >  2:2731732/1‑150 (MQ=255)
                                                                                                                                           tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAATGCGGATTAGTTGGGAAGTTCAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGATACGAACCTTCGAAGTCGATGTCGGCAGAt          >  1:2459046/1‑150 (MQ=21)
                                                                                                                                             gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt        <  1:641540/150‑1 (MQ=255)
                                                                                                                                              cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTa       <  1:1725808/150‑1 (MQ=255)
                                                                                                                                               gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      >  1:1212703/1‑150 (MQ=255)
                                                                                                                                               gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  2:500818/63‑1 (MQ=25)
                                                                                                                                               gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  2:500804/63‑1 (MQ=25)
                                                                                                                                                cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     >  2:1197039/1‑150 (MQ=255)
                                                                                                                                                cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACCATCGAAGTCGATGACGGCAGATTTACa     >  1:3526538/1‑150 (MQ=255)
                                                                                                                                                 tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                 <  2:1975804/57‑1 (MQ=255)
                                                                                                                                                   gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  <  1:3448534/150‑1 (MQ=255)
                                                                                                                                                   gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  2:1234801/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287002‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: