Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,520,943 A→T 6.3% intergenic (+16/+20) mcbR → / ← yncD colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled/putative iron outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,9430AT 6.3% 582.9 / 20.0 167intergenic (+16/+20)mcbR/yncDcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled/putative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (84/72);  new base T (5/6);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

GGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGAC  >  NC_000913/1520798‑1521091
                                                                                                                                                 |                                                                                                                                                    
ggAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATAc                                                                                                                                                  <  2:2001043/150‑1 (MQ=255)
ggAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTATTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATAc                                                                                                                                                  <  2:2000942/150‑1 (MQ=255)
 gAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATAcc                                                                                                                                                 <  1:911168/150‑1 (MQ=255)
  aGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCg                                                                                                                                                <  2:3582045/150‑1 (MQ=255)
  aGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCg                                                                                                                                                <  2:1895768/150‑1 (MQ=255)
     atatAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGc                                                                                                                                             >  1:222516/1‑150 (MQ=255)
      tataGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCa                                                                                                                                            >  1:1239729/1‑150 (MQ=255)
        taGAAAACTATCATCTTTTACTAGCTTCTTTAAAAGAAAAAGACAAATATTTATGAAGACTTTGTATTGCTGAAATTATGAAACAAAATTTTGCTATTTTATACCATCAATACAATCGTTAAAGTAAATTCCCGGTATTTGCCGGGCAtt                                                                                                                                          <  1:800514/150‑2 (MQ=255)
        taGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGTATTTGCCGGGCAtt                                                                                                                                          <  1:3214443/150‑2 (MQ=255)
        taGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGTATTTGCCGGGCAtt                                                                                                                                          <  1:2738484/150‑2 (MQ=255)
         aGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATAGAATCGTTAAAGTAAATGCCCGGCAATTACAGGGCATct                                                                                                                                         >  2:844725/1‑149 (MQ=255)
         aGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCa                                                                                                                                         >  2:1258744/1‑150 (MQ=255)
          gAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCaa                                                                                                                                        <  2:2061721/150‑1 (MQ=255)
           aaaaCTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAg                                                                                                                                       <  1:1092242/150‑1 (MQ=255)
             aaCTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGt                                                                                                                                     <  1:3264258/150‑1 (MQ=255)
               cTATCATCTGTGACTCGCTGCGTTAAAAGCAACAGACAACGAGGGATGCAGACATTGTCTTGCTGAAATTCTGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTaa                                                                                                                                   <  2:2793792/150‑1 (MQ=255)
                tATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAAt                                                                                                                                  <  1:2652837/150‑1 (MQ=255)
                    atcTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACt                                                                                                                              >  2:367596/1‑150 (MQ=255)
                      cTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCa                                                                                                                            >  1:3575945/1‑150 (MQ=255)
                        gTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCaaa                                                                                                                          >  1:353788/1‑150 (MQ=255)
                         ttACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAt                                                                                                                         <  1:3361528/150‑1 (MQ=255)
                           aCTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCGGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtct                                                                                                                       >  2:2899999/1‑150 (MQ=255)
                           aCTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACAAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtct                                                                                                                       >  1:3108624/1‑150 (MQ=255)
                            cTCGCTGCGTTAAAAGCTAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtctc                                                                                                                      <  2:3566571/150‑1 (MQ=255)
                              cGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCa                                                                                                                    <  1:3489522/150‑1 (MQ=255)
                              cGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCa                                                                                                                    <  1:3695022/150‑1 (MQ=255)
                                   cGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCaa                                                                                                               <  1:2868/150‑1 (MQ=255)
                                      tAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAatat                                                                                                            <  1:1970946/150‑1 (MQ=255)
                                        aaaGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTc                                                                                                          <  2:1994614/150‑1 (MQ=255)
                                        aaaGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTc                                                                                                          >  2:2104231/1‑150 (MQ=255)
                                            cAAAAGACACAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      <  1:1047975/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      >  2:3611052/1‑150 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      <  2:1445072/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      <  2:3033815/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      >  2:2922354/1‑150 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      <  1:2964247/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                      >  2:399451/1‑150 (MQ=255)
                                             aaaaGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGcc                                                                                                     >  2:2189884/1‑150 (MQ=255)
                                             aaaaGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGcc                                                                                                     >  1:2259841/1‑150 (MQ=255)
                                             aaaaGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGcc                                                                                                     >  1:1848834/1‑150 (MQ=255)
                                                      agagGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATa                                                                                            <  2:2196970/150‑1 (MQ=255)
                                                                aGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCg                                                                                  >  2:208404/1‑150 (MQ=255)
                                                                aGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCg                                                                                  <  1:2689058/150‑1 (MQ=255)
                                                                aGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCg                                                                                  <  1:2689062/150‑1 (MQ=255)
                                                                  aCATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCggg                                                                                >  2:1418185/1‑150 (MQ=255)
                                                                     ttGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGa                                                                             >  1:1408916/1‑150 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCTCGCAATATGCATGCCGACACCATAATTTCGTCGGGGCGaa                                                                            >  1:2295663/1‑150 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGaa                                                                            <  2:1717580/150‑1 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGaa                                                                            <  1:412756/150‑1 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGaa                                                                            <  1:1014336/150‑1 (MQ=255)
                                                                         cTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGt                                                                         <  1:3885280/150‑1 (MQ=255)
                                                                         cTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGt                                                                         >  1:3878279/1‑150 (MQ=255)
                                                                         cTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGt                                                                         <  2:519153/150‑1 (MQ=255)
                                                                             cttaaaTTATGCAACAAAATATTGCTATTATATACCAGCAATACAATCGTTAAACTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGt                                                                     <  2:3101615/147‑1 (MQ=255)
                                                                              tGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCCAGGTAATAACTCAAATCTCCACGCAATATTCATGCCGACACCATAATATCGTCCGGGCGAAGGTTCGTa                                                                    >  1:2237846/1‑150 (MQ=255)
                                                                              tGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTa                                                                    >  2:705457/1‑150 (MQ=255)
                                                                               gAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGACGGTTCGTaa                                                                   >  2:636896/1‑150 (MQ=255)
                                                                               gAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTaa                                                                   >  2:1652566/1‑150 (MQ=255)
                                                                               gAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTaa                                                                   >  2:3769479/1‑150 (MQ=255)
                                                                                aaaTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAat                                                                  >  2:2618635/1‑150 (MQ=255)
                                                                                  aTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                                                                >  2:3084700/1‑150 (MQ=255)
                                                                                  aTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                                                                >  2:2407446/1‑150 (MQ=255)
                                                                                   ttATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATc                                                               <  1:844725/150‑1 (MQ=255)
                                                                                     aTGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGc                                                             <  1:1293705/150‑1 (MQ=255)
                                                                                       gCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGccc                                                           >  2:3930093/1‑150 (MQ=255)
                                                                                        caacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCg                                                          <  2:1445742/150‑1 (MQ=255)
                                                                                        aaacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCg                                                          <  1:1694404/149‑1 (MQ=255)
                                                                                         aacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGt                                                         >  1:3336833/1‑150 (MQ=255)
                                                                                         aacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGt                                                         >  1:707611/1‑150 (MQ=255)
                                                                                          acaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGtt                                                        >  1:582972/1‑150 (MQ=255)
                                                                                          acaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGtt                                                        >  1:582968/1‑150 (MQ=255)
                                                                                          acaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGtt                                                        >  2:2495992/1‑150 (MQ=255)
                                                                                           caaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGttt                                                       >  1:2441213/1‑150 (MQ=255)
                                                                                           caaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGttt                                                       >  1:2441735/1‑150 (MQ=255)
                                                                                            aaaaTATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATGTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTg                                                      >  1:3496828/1‑150 (MQ=255)
                                                                                             aaaTATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGa                                                     <  1:2573281/150‑1 (MQ=255)
                                                                                               atatCGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACt                                                   >  2:1292332/1‑150 (MQ=255)
                                                                                                     cTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGa                                             <  1:2104231/150‑1 (MQ=255)
                                                                                                     cTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGa                                             >  2:2255533/1‑150 (MQ=255)
                                                                                                       aTTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACa                                           <  1:1301048/150‑1 (MQ=255)
                                                                                                           tataCCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGa                                       <  2:3848877/150‑1 (MQ=255)
                                                                                                           tataCCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGa                                       <  2:129303/150‑1 (MQ=255)
                                                                                                           tataCCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGa                                       <  2:2822411/150‑1 (MQ=255)
                                                                                                               ccAGCATTACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGa                                   <  2:1935671/150‑1 (MQ=255)
                                                                                                                 aGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAc                                 >  1:1805743/1‑150 (MQ=255)
                                                                                                                 aGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAc                                 <  1:1541841/150‑1 (MQ=255)
                                                                                                                  gCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAcc                                >  1:1127685/1‑150 (MQ=255)
                                                                                                                            cGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCt                      <  2:339263/150‑1 (MQ=255)
                                                                                                                                   gTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGa               >  2:2335498/1‑150 (MQ=255)
                                                                                                                                    ttgaTGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTa                   >  2:1761971/4‑145 (MQ=255)
                                                                                                                                     aaaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAAt             <  1:208404/150‑1 (MQ=255)
                                                                                                                                        tGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATaaa          >  2:646549/1‑150 (MQ=255)
                                                                                                                                         gCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAAt         >  1:1178624/1‑150 (MQ=255)
                                                                                                                                            cGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTAt      >  1:1671594/1‑150 (MQ=255)
                                                                                                                                             ggCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATc     <  1:2899999/150‑1 (MQ=255)
                                                                                                                                              gCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCg    >  1:3157136/1‑150 (MQ=255)
                                                                                                                                               cAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa   <  2:2867650/150‑1 (MQ=255)
                                                                                                                                               cAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa   >  1:382547/1‑150 (MQ=255)
                                                                                                                                               cAAAGACCGGGCATCAAGGTAATTCCTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa   <  2:1894640/150‑1 (MQ=255)
                                                                                                                                                aaaTACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGac  >  2:2010506/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGAC  >  NC_000913/1520798‑1521091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: