Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,520,944 A→T 7.1% intergenic (+17/+19) mcbR → / ← yncD colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled/putative iron outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,9440AT 7.1% 572.6 / 24.7 167intergenic (+17/+19)mcbR/yncDcolanic acid and biofilm gene transcriptional regulator, MqsR‑controlled/putative iron outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base A (83/72);  new base T (6/6);  total (89/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

Only 100 of 169 total aligned reads displayed.

GGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACAC  >  NC_000913/1520798‑1521093
                                                                                                                                                  |                                                                                                                                                     
ggAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTATTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATAc                                                                                                                                                    <  2:2000942/150‑1 (MQ=255)
  aGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCg                                                                                                                                                  <  2:1895768/150‑1 (MQ=255)
  aGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCg                                                                                                                                                  <  2:2176707/150‑1 (MQ=255)
  aGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCg                                                                                                                                                  <  2:3582045/150‑1 (MQ=255)
   gCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCgg                                                                                                                                                 <  2:200118/150‑1 (MQ=255)
     atatAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGTATTTGCCGGGc                                                                                                                                               <  2:1100757/150‑1 (MQ=255)
     atatAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGc                                                                                                                                               >  1:222516/1‑150 (MQ=255)
      tataGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCa                                                                                                                                              >  1:1239729/1‑150 (MQ=255)
        taGAAAACTATCATCTTTTACTAGCTTCTTTAAAAGAAAAAGACAAATATTTATGAAGACTTTGTATTGCTGAAATTATGAAACAAAATTTTGCTATTTTATACCATCAATACAATCGTTAAAGTAAATTCCCGGTATTTGCCGGGCAtt                                                                                                                                            <  1:800514/150‑2 (MQ=255)
        taGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGTATTTGCCGGGCAtt                                                                                                                                            <  1:3214443/150‑2 (MQ=255)
        taGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGTATTTGCCGGGCAtt                                                                                                                                            <  1:2738484/150‑2 (MQ=255)
         aGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCCTCa                                                                                                                                           >  2:3236122/1‑150 (MQ=255)
         aGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCa                                                                                                                                           >  2:1258744/1‑150 (MQ=255)
          gAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCaa                                                                                                                                          <  2:2061721/150‑1 (MQ=255)
           aaaaCTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAg                                                                                                                                         <  1:1092242/150‑1 (MQ=255)
             aaCTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGt                                                                                                                                       <  1:3264258/150‑1 (MQ=255)
               cTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTaa                                                                                                                                     <  2:2793780/150‑1 (MQ=255)
               cTATCATCTGTGACTCGCTGCGTTAAAAGCAACAGACAACGAGGGATGCAGACATTGTCTTGCTGAAATTCTGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTaa                                                                                                                                     <  2:2793792/150‑1 (MQ=255)
                tATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAAt                                                                                                                                    <  1:2652837/150‑1 (MQ=255)
                  tcatcTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTa                                                                                                                                  >  1:2544209/1‑150 (MQ=255)
                    atcTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACt                                                                                                                                >  2:1409986/1‑150 (MQ=255)
                     tcTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTc                                                                                                                               >  1:2177039/1‑150 (MQ=255)
                     tcTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTc                                                                                                                               >  1:3703468/1‑150 (MQ=255)
                      cTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCa                                                                                                                              >  1:3575945/1‑150 (MQ=255)
                        gTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCaaa                                                                                                                            >  1:353788/1‑150 (MQ=255)
                         ttACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAt                                                                                                                           <  1:3361528/150‑1 (MQ=255)
                          tACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtc                                                                                                                          <  2:3831827/150‑1 (MQ=255)
                           aCTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCGGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtct                                                                                                                         >  2:2899999/1‑150 (MQ=255)
                           aCTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACAAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAAtct                                                                                                                         >  1:3108624/1‑150 (MQ=255)
                              cGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCa                                                                                                                      <  1:3489522/150‑1 (MQ=255)
                               gCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCAc                                                                                                                     >  2:3911546/1‑150 (MQ=255)
                                  gCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCa                                                                                                                  <  2:28421/150‑1 (MQ=255)
                                   cGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCaa                                                                                                                 <  1:2868/150‑1 (MQ=255)
                                    gTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAat                                                                                                                >  1:3916901/1‑150 (MQ=255)
                                      tAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAatat                                                                                                              <  1:1970946/150‑1 (MQ=255)
                                        aaaGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTc                                                                                                            <  2:197703/150‑1 (MQ=255)
                                        aaaGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTc                                                                                                            <  2:1994660/150‑1 (MQ=255)
                                            cAAAAGACACAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        <  1:1047975/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        <  1:2964247/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        >  2:1622535/1‑150 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        <  2:1445072/150‑1 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        >  1:552372/1‑150 (MQ=255)
                                            cAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        >  2:399451/1‑150 (MQ=255)
                                            cAAAAGACAAAGAAGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGc                                                                                                        >  2:3604242/1‑150 (MQ=255)
                                             aaaaGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGcc                                                                                                       >  1:1848834/1‑150 (MQ=255)
                                             aaaaGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGcc                                                                                                       >  2:2189884/1‑150 (MQ=255)
                                                          ggATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAAttt                                                                                          >  2:167739/1‑150 (MQ=255)
                                                                aGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCg                                                                                    <  1:2689062/150‑1 (MQ=255)
                                                                aGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCg                                                                                    <  1:2689058/150‑1 (MQ=255)
                                                                  aCATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCggg                                                                                  <  2:2876363/150‑1 (MQ=255)
                                                                  aCATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCggg                                                                                  >  2:1418185/1‑150 (MQ=255)
                                                                     ttGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGa                                                                               >  1:1408916/1‑150 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCTCGCAATATGCATGCCGACACCATAATTTCGTCGGGGCGaa                                                                              >  1:2295663/1‑150 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGaa                                                                              <  1:1014336/150‑1 (MQ=255)
                                                                      tGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGaa                                                                              <  2:1717580/150‑1 (MQ=255)
                                                                         cTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGt                                                                           >  1:3878279/1‑150 (MQ=255)
                                                                              tGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCCAGGTAATAACTCAAATCTCCACGCAATATTCATGCCGACACCATAATATCGTCCGGGCGAAGGTTCGTa                                                                      >  1:2237846/1‑150 (MQ=255)
                                                                              tGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTa                                                                      >  1:3530918/1‑150 (MQ=255)
                                                                              tGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTa                                                                      <  1:2925468/150‑1 (MQ=255)
                                                                               gAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTaa                                                                     >  2:636841/1‑150 (MQ=255)
                                                                               gAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTaa                                                                     >  2:3769479/1‑150 (MQ=255)
                                                                                  aTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                                                                  >  2:2407300/1‑150 (MQ=255)
                                                                                  aTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                                                                  >  2:2407446/1‑150 (MQ=255)
                                                                                  aTTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAatat                                                                  >  2:3084700/1‑150 (MQ=255)
                                                                                   ttATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATc                                                                 <  2:2090643/150‑1 (MQ=255)
                                                                                   ttATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATc                                                                 <  1:844725/150‑1 (MQ=255)
                                                                                     aTGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGc                                                               <  1:1293705/150‑1 (MQ=255)
                                                                                        aaacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCg                                                            <  1:1694404/149‑1 (MQ=255)
                                                                                         aacaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGt                                                           >  2:2038417/1‑150 (MQ=255)
                                                                                          acaaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGtt                                                          >  1:582968/1‑150 (MQ=255)
                                                                                           caaAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGttt                                                         >  1:2441213/1‑150 (MQ=255)
                                                                                            aaaaTATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTg                                                        >  2:2311237/1‑150 (MQ=255)
                                                                                             aaaTATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGa                                                       <  1:2573281/150‑1 (MQ=255)
                                                                                               atatCGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACt                                                     >  2:1292332/1‑150 (MQ=255)
                                                                                                   tGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCAtt                                                 <  2:2544209/150‑1 (MQ=255)
                                                                                                      tATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGAc                                              >  2:1796739/1‑150 (MQ=255)
                                                                                                       aTTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACa                                             <  1:1301048/150‑1 (MQ=255)
                                                                                                               ccAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGa                                     <  2:294946/150‑1 (MQ=255)
                                                                                                                 agaaaTACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATAAACGTAATTACTCAAATCTCCACGCAATATTCATGCCGAAACCATAATAACGTCAGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCAATGACAATGACAGAAc                                   >  2:225286/4‑150 (MQ=255)
                                                                                                                 aGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAc                                   <  1:1541841/150‑1 (MQ=255)
                                                                                                                 aGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAc                                   >  1:1805743/1‑150 (MQ=255)
                                                                                                                  gCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAAcc                                  >  1:1127685/1‑150 (MQ=255)
                                                                                                                               tAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCAAAATTTCGTCCGGGCGAAGGTTCGTAATATAGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTa                     >  1:3350997/1‑150 (MQ=255)
                                                                                                                                aaaGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTAt                    <  2:3637470/150‑1 (MQ=255)
                                                                                                                                     tgaTGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAAt               >  2:689462/3‑150 (MQ=255)
                                                                                                                                     aaaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAAt               <  1:208404/150‑1 (MQ=255)
                                                                                                                                      gaTGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATa              <  1:689462/149‑1 (MQ=255)
                                                                                                                                      aaTGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATa              <  1:2286065/150‑1 (MQ=255)
                                                                                                                                        tGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATaaa            >  2:417930/1‑150 (MQ=255)
                                                                                                                                        tGCCCGGTATTTGCCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATaaa            >  2:646549/1‑150 (MQ=255)
                                                                                                                                        tGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATaaa            >  2:3057474/1‑150 (MQ=255)
                                                                                                                                         gCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAAt           >  1:1178624/1‑150 (MQ=255)
                                                                                                                                         gCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAAt           >  1:3200870/1‑150 (MQ=255)
                                                                                                                                            cGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTAt        >  1:1671594/1‑150 (MQ=255)
                                                                                                                                            cGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTAt        <  2:353788/150‑1 (MQ=255)
                                                                                                                                             ggCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATc       <  2:835723/150‑1 (MQ=255)
                                                                                                                                               cAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa     <  2:1894679/150‑1 (MQ=255)
                                                                                                                                               cAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa     >  1:382547/1‑150 (MQ=255)
                                                                                                                                               cAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGa     >  1:784361/1‑150 (MQ=255)
                                                                                                                                                  aTACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGacac  <  2:931280/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGAGCATATAGAAAACTATCATCTGTTACTCGCTGCGTTAAAAGCAAAAGACAAAGAGGGATGCAGACATTGTCTTGCTGAAATTATGCAACAAAATATTGCTATTTTATACCAGCAATACAATCGTTAAAGTAAATGCCCGGCAAATACCGGGCATCAAGGTAATTACTCAAATCTCCACGCAATATTCATGCCGACACCATAATTTCGTCCGGGCGAAGGTTCGTAATATCGCCCGTTTGACTCATTGACAATGACAGAACCAACGTATTCTTTATCGAATAAATTATCGACAC  >  NC_000913/1520798‑1521093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: