| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,581 | A→C | 14.1% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,581 | 0 | A | C | 14.1% | 150.1 / 10.7 | 96 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (29/52); new base C (2/13); total (31/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAA > NC_000913/164431‑164727 | aCCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCa > 2:1030688/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCAt > 1:1938145/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:1882637/150‑1 (MQ=255) gAGGTGAAAAAAGAGATTAATGGGCTTTATCCAAAACTTGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAATGTATTCGTATGTTTGTTTTT‑CTCCCTCTCCC‑TGTGGGTGTGGGCCGGGGTGAGGGCATCAg < 2:1797954/150‑1 (MQ=37) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 2:1242976/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg > 2:1320895/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg > 1:2104694/1‑150 (MQ=255) cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa > 1:1463001/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGTGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc > 2:3098586/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc > 1:979119/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACgg > 1:903279/1‑149 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt > 1:903537/1‑150 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACggg > 2:3611371/1‑148 (MQ=255) agtgttGAATGGGCTTTTTCCAAAACATTTGTGGCGGGACGACCCGGAAAATCCTGCACGGAGGGGTGGGACGAAATTGTTTTGGTATGGTTGTTTTT‑CTCCCTCTCCC‑TGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtc < 2:1161828/145‑1 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgc > 2:255218/1‑148 (MQ=255) gagaTTAATGGGGGTTATCCAAAAAAGGTCTGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAATTATTGGTAAGTTTGGTTTT‑CTCCCTCTCCC‑TGTGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtca < 2:3128769/150‑1 (MQ=37) gaTGAACGGGCTTTATCCCAAAATGGTGTGGCGGGACGACCGGGCAAATACTGCACCGACGCGACGGACGAAAATTTATTCGTTAGGTTGGTTTT‑CTCCCTCTCCG‑TGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc < 2:3479992/150‑1 (MQ=25) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtggggcc > 1:2013501/1‑144 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccccc > 1:2616086/1‑148 (MQ=255) aatgggCTTTATCCCAAACTTGTCGGGCGGGACGACCGGGAAAATACTGCACCGACGCGACGTACTAAAATGTTTTCTTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGTTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 2:896744/147‑1 (MQ=25) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcccccaca > 2:2002495/1‑150 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccgca > 2:2002497/1‑150 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccaca > 2:2003997/1‑150 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccaca > 2:2525641/1‑150 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccaca > 2:2525679/1‑150 (MQ=255) cgggCGTTTTCCCAAACATGTCTGGCCGGACGACCCGGAAATTACTGCCCCGACGCGACGGCCGAATATGTATTGGTATGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGATTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 2:2041348/149‑1 (MQ=37) cgggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAAAGTTTTCGTATGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcaccctcacc < 2:331687/149‑1 (MQ=38) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTATT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCaaaa > 1:619019/1‑149 (MQ=38) ttATCCCAAACCTGTGTGGCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAATGTATTCGTATGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:2746345/150‑1 (MQ=38) aTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGCCGGACGAAAAAGTATTCGTATGGTTGGTTTG‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct < 2:2002264/150‑1 (MQ=255) aTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGGAGTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCCGCCGCGCGCACGTGCCCCCTCACCCCAAccca > 2:1937703/1‑149 (MQ=38) ccccAAACCTGTCGGGCGGGACGACCGGCAAAATACTGCCCCGCCGCGACGGACGAAAATTTTTTCGTATGGTTGTTTTT‑CGCCCTCGCCG‑TGGGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc < 2:2013461/149‑1 (MQ=255) cAACCATGTCTGGCGGGACGCCCCGGAAATTACTGCACCGACGCGACGGACTAAAAATTATTCGTATGGTTGTTTTT‑CTCCCTCTCCC‑GGTGGTTGTGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:2404094/150‑1 (MQ=255) cAAACATGTCTGGCCGTACGACCCGGCAAATACTGCACCGACGCGAGGGACTAAAATGTATTCGTATGGTTGTTTTT‑CTCCCTCTCCG‑TGTGGGCGTGGGCGGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:221682/150‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAAaccccacc > 1:3097773/1‑145 (MQ=37) cAAACATGTCGGGCCGGACGACCCGGCAACTACTGCACCGCCGCGAGGGACGAAAATTTTTTCGTAGGGTTGGTTTT‑CGCCCTCGCCG‑GGTGGTTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:3811198/150‑1 (MQ=255) aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCCAAaccacaacc > 1:1413925/1‑144 (MQ=37) ctGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGCCGGACGAAAAAGTTTTCGTATGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:3760383/149‑1 (MQ=255) ctGTCTGGCCGCACGACCCGTCAAATACTGCACCGCCGCCACGCACGAAAAAGTATTCTTACGGTGGGTTTT‑CCCCCTCCCCC‑TGTGGGAGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:3723340/149‑1 (MQ=37) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:2959558/150‑1 (MQ=255) aTGTCTGGCCGGACGACCCGGAAAATACTGAACCGACGCTACGGACGAAAAATTATTCGTAAGTTTGGTTTT‑CTCCCTCTCCC‑TGTGGGCGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:3760405/150‑1 (MQ=38) aTGTCTGGCCGGACGACCCGCCAAATACTGCACCGACGCGACGGACCAAAAAGTTTTCGTATGGTTGGTTTT‑CTCCCCCTCCG‑GGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 1:3539734/150‑1 (MQ=255) tgggggcccGGACGCCCCGGAAAATACTGCCCCGCCGCGCCGGACTAAAATGTATTGGTATGTTTGTTTTT‑CCCCCTCCCCG‑GGTGGGTGGGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3676993/143‑1 (MQ=255) tcgggCCGGACGACCCGGAAAAGCCTCCACCGCCGCGACGCACTAAAATTTATTCTTTTGTTTGGTTTT‑CCCCCTCTCCG‑TGTGGGCTCGGGGGGGGGGTCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAggg < 1:507833/147‑1 (MQ=255) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCCCACCCAAACCCACACCCACAAggggg > 1:2858351/1‑148 (MQ=37) gccggccgCCCCGGCAAATCCGGCCCCGCCGCGTCGTACTAAAATTTTTTGTTTTGGTTGGTTTT‑CTCCCTCTCCC‑TGGGGTTGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:676648/150‑1 (MQ=255) cgcccgacCCGCAAAAAACGCCACCGACGCGCCGGACCAAAATGTATTCTTATGGTTGTTTTC‑CCCCCTCCCCG‑TGGGGGTGCGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:1759747/146‑1 (MQ=255) cGGACGACCCGCCAAATCCGCCCCCGCCGCGCGGTCCTAAAATTTATTCGTTTGGTGGGTTTG‑CGCCCCCGCCC‑TGGGGGGGCGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 1:587471/150‑1 (MQ=255) gacgacCCGGAAAATACTGCACCGCCGCGACGGACGAAAAATTATTCGTACGTTGGGTTTT‑CTCCCTCTCCC‑TGTGGTAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:2786666/150‑1 (MQ=255) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTT‑CTCCCTCCCCC‑TGTGGGTGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc < 2:3804391/150‑1 (MQ=39) cccccGCCAAAAACTGCCCCGACGCGCCGCACGAAAATGTATTCGTACGGTTGGTTTA‑CTCCCTCCCCG‑TGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg < 2:2104694/148‑6 (MQ=255) ccccttaaaaTACTGCACCGCCGCGCCGGACTAATATTTTTTCTTTTGGTTGTTTTT‑CTCCCTCTCCC‑TGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa < 1:3797072/143‑1 (MQ=25) cGGCAAATCCTGCACCGACGCGACGGACTAAAAAGTATTCGTACGGTTGGTTTT‑CGCCCTCCCCC‑TGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCg < 2:2088745/150‑1 (MQ=38) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCCCACCCCAAACCACAACCACAAGGGAGAGGGGGCCGATCGAGcc > 1:2306929/1‑149 (MQ=37) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 2:2933384/150‑1 (MQ=255) cccgccggcgccgGACTAATATGTTTTGGTTTGGTGGTTTGT‑CTCCCTCACCG‑TGGGGGCGGGGGGGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:3652981/143‑1 (MQ=255) cACCGAGGCGAGGGACTAAAATTTTTTCTTAGGTTTGGTTTT‑CTCCCTCCCCG‑TGTGGGTGTGGGGGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:1937369/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCACCAAACCCACAACCACAACCGGGGGGGGGCCCAGAGAGCACaaaaatt > 1:349043/1‑146 (MQ=16) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCCAAACCACTCCCACAAGGGAGGCGGGGCCGCTCCAGCCCAAAattt > 2:3525739/1‑150 (MQ=25) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCCCCCCCCAACCCCCACCCGCAAGGGGGTGGGGGCCCCACGAGCGCAAAattt > 1:1284691/1‑150 (MQ=21) cggtcgcgaggGACTAAAATGT‑TTGGTTATGTTGGTTTTACCCCATCTCCC‑TGTGGGCGTGGCCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGGACAAATTTTga < 2:3149839/146‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3608909/150‑1 (MQ=255) acggactAAAATGTATTCGTACGGTTGGTTTT‑CGCCCCCTCCG‑GGTGGGAGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:1185122/150‑1 (MQ=255) acggactAAAAAGTTTTCGTAAGGTGGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCACTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:3227098/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACAACCCAACCCCAGCCCCCAAGCGCGAGGGGGCCCGGAGCGCCCACAATTTGAGTGATAtcttatta > 2:1962383/1‑149 (MQ=16) aacgTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 2:2968643/147‑1 (MQ=255) gtctccGTATGGTTGGTTTT‑CGCCCACGCCC‑GGTGGGAGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGCTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:313143/145‑1 (MQ=255) tttCGTATGGTTGGTTTT‑CTCCCCCGCCC‑TGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:3097773/149‑1 (MQ=255) aTTCGAATGGTGGTTTTT‑CCCCCCCGCCC‑GGTGGTCGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:41483/150‑1 (MQ=255) gttgggttgtttTT‑CTCCCTCGCCG‑GGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 2:177499/146‑1 (MQ=255) gTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:1656015/150‑1 (MQ=255) ttggttggttTT‑CTCCCTCTCCC‑TGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:2303486/148‑1 (MQ=255) acggttggttTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:3872037/148‑1 (MQ=255) cggtttgttTT‑CCCCCCCCCCCGGGTGGGTGTGGGCGGGGGGGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:687637/149‑1 (MQ=255) ggttTT‑CCCCCTCCCCC‑TGTGGGTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGTAGAACa < 1:3736202/150‑1 (MQ=255) ctccctctccc‑tGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt < 1:1672085/150‑1 (MQ=255) tccctctcgc‑tGGGGTAGTGGGTCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGTGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:2928075/150‑1 (MQ=255) tccctctcct‑gGGGGGCGTGGGGGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACTGAAGAACAGAAAATc < 1:138658/150‑1 (MQ=255) accctctccg‑tGTGGGTGTGGGCGGGGTTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:1359556/149‑1 (MQ=255) ccctctccc‑tGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCGAACCCTCTCCCTCCAGGGGGAGGGGCCCGATCGAGCACCAATTTTGAGAGATATATTATTCTGTCTTGTAACAGAAGAACAGAAAATCg > 2:2317263/1‑150 (MQ=38) cctctcct‑gGGGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:3187857/150‑1 (MQ=255) cctctccc‑tGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCAAACCCAAAACCACACCCGCAAGGGGGAGGGGACCCAAAGAGCACAAATTTTGAGAGGTAAAATATTCTGTCTTTTAACAGAATAACAGAAAAtacg > 2:998417/1‑147 (MQ=21) ccc‑tGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 2:2309464/150‑1 (MQ=255) cgtgtgGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 1:2520366/148‑1 (MQ=255) ggggcgcgggCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:3082790/143‑1 (MQ=255) ggtgtgggCGGGGGTGCGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:3023149/145‑1 (MQ=255) ggcgcgggCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:398528/145‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACAACCACCAGGGGGAGGGGGCCGAAAGAGCACAAAATTTGTGAGATATATTATTATGTCTTGTTACAGAACAAAAAAAAAAAGGGCtttttagccg > 2:3949141/1‑144 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:480455/150‑1 (MQ=255) ggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:299322/150‑1 (MQ=255) gttGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:3038174/148‑1 (MQ=255) ggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:3038181/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:2972620/150‑1 (MQ=255) gggcgggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgag < 1:490833/146‑1 (MQ=255) gggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 2:1285166/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:2381407/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:2655548/150‑1 (MQ=255) | ACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTT‑CTCCCTCTCCC‑TGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAA > NC_000913/164431‑164727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |