Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 176,978 A→T 5.6% intergenic (+24/+23) erpA → / ← yadS iron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913176,9780AT 5.6% 737.0 / 25.1 212intergenic (+24/+23)erpA/yadSiron‑sulfur cluster insertion protein/UPF0126 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (121/79);  new base T (8/4);  total (129/83)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 221 total aligned reads displayed.

ATGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTG  >  NC_000913/176829‑177127
                                                                                                                                                     |                                                                                                                                                     
      cTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATcc                                                                                                                                                 >  1:3906955/1‑150 (MQ=255)
        gCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                               <  1:3310843/150‑1 (MQ=255)
        gCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                               >  2:2190545/1‑150 (MQ=255)
         cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGc                                                                                                                                              >  1:32284/1‑150 (MQ=255)
         cAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGc                                                                                                                                              <  1:1962799/150‑1 (MQ=255)
          aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             <  2:3298796/150‑1 (MQ=255)
          aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             <  2:3653332/150‑1 (MQ=255)
          aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             <  2:1719356/150‑1 (MQ=255)
          aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             <  2:2159045/150‑1 (MQ=255)
          aaTATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGca                                                                                                                                             <  1:2154029/150‑1 (MQ=255)
            tatCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAGTTTATCCGGcaca                                                                                                                                           <  1:1304060/150‑1 (MQ=255)
                    cggcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctct                                                                                                                                   <  2:1931387/150‑1 (MQ=255)
                    cggcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctct                                                                                                                                   <  2:3879923/150‑1 (MQ=255)
                    cggcggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTtctct                                                                                                                                   <  2:522263/150‑1 (MQ=255)
                       cggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATATAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCtt                                                                                                                                >  1:1645800/1‑150 (MQ=255)
                        ggTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTc                                                                                                                               >  2:3069252/1‑150 (MQ=255)
                         gTTCCGTTGATTATAACCAAGGTCTGGAAGGTTCTCGTTTCATAGTGACCAACCCCAAAGCCAAAAGCACCAGCGGTTGCGGTTCTTCCTTTAGTATCTAAACTGGTGTTCATGTGCCGGATAAACAATACGGCACAACTTCTCTCTTCa                                                                                                                              >  1:1402679/1‑150 (MQ=255)
                              gTTGATTATACCGAATGTCTGGAATGTTCTCGTATCATCGTGACCAACACGAACGCGAAAAGCACCTGCGGGTGCGGTACTTCCTTTAGTATCTAATCTGTATTTCATGTGCAGGATAAACAATACGGCGCATCTTCTCACATCAACGcc                                                                                                                         >  2:2904226/1‑150 (MQ=255)
                              gTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                         >  1:2065309/1‑150 (MQ=255)
                              gTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGcc                                                                                                                         >  1:490056/1‑150 (MQ=255)
                               ttGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGccc                                                                                                                        >  2:2080697/1‑150 (MQ=255)
                               ttGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTCTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGccc                                                                                                                        >  1:2496513/1‑150 (MQ=255)
                                     ataCCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTATTATCTAATCTTTTTTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATtctc                                                                                                                  <  2:1389605/150‑1 (MQ=255)
                                        ccGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATc                                                                                                               >  2:3760751/1‑150 (MQ=255)
                                          gAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCa                                                                                                             >  1:1132030/1‑150 (MQ=255)
                                          gAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCa                                                                                                             <  2:2752847/150‑1 (MQ=255)
                                           aaGGTCTGGAAGGGTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAg                                                                                                            <  2:279802/150‑1 (MQ=255)
                                              gtgtGGATGTTTCTGTTTTCATGGTCACCAACCCGACCGCTAAAAGCACCTGGGGTTGGGTTTCTTACTTTAGTATATAATGTTTTGTTCTTGGGCCGTATAAACTATCGGCCACATCTTCTCTCTTCAACGCCCATTCTCATCCAgcgc                                                                                                         <  1:1750984/147‑1 (MQ=255)
                                              gTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAgcgc                                                                                                         >  1:3895800/1‑150 (MQ=255)
                                               tCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCATCTCTCTTCAACGACCATTATCATCCAGCGCa                                                                                                        >  1:2331005/1‑150 (MQ=255)
                                                cTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGCTAAACTATCCGGCACATCTTCTCTCTTCACCGCCCATTCTCCTCCAGCGCac                                                                                                       >  2:3146273/1‑149 (MQ=255)
                                                 tGGTAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATATACTATCCGGCACATCTTCTCTCTTCAACGCGCATTCTCATCCTGCGCaaa                                                                                                      >  1:2561260/1‑150 (MQ=255)
                                                 tGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCaaa                                                                                                      >  2:2372698/1‑150 (MQ=255)
                                                         tctcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCgg                                                                                                                                               <  1:2742276/101‑1 (MQ=255)
                                                          ctcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTGTAGTATCTAATCGGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGtcg                                                                                             >  2:2298915/1‑148 (MQ=255)
                                                           tcGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGc                                                                                            >  2:2542401/1‑150 (MQ=255)
                                                             gTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCtt                                                                                          >  1:1003273/1‑150 (MQ=255)
                                                               ttCATCGTCACCACCCCCAACGCTAAAAGCACCGGGGGTTGGGGTTCTTCCTTTTGTATCTAATCTTTTTTTCTTGTGCGGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTaa                                                                                        <  1:1116551/150‑1 (MQ=255)
                                                                tCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAg                                                                                       >  2:928740/1‑150 (MQ=255)
                                                                tCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAg                                                                                       >  2:2244624/1‑150 (MQ=255)
                                                                tCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAg                                                                                       <  2:867552/150‑1 (MQ=255)
                                                                     gTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGcc                                                                                  >  2:837534/1‑150 (MQ=255)
                                                                     gTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCGTCCTGTAGTAGCTAATCTGGTGTTCATGTGCCGGATAAACTATCCGGCACCTCTTCTCTCGGCCACGCCCAGTCTCATCCAGCGCACCCGGCGGTAGCTGACGATGcc                                                                                  >  2:897238/1‑150 (MQ=255)
                                                                       gACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCaa                                                                                >  2:2414020/1‑150 (MQ=255)
                                                                         ccAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACg                                                                              >  1:3654262/1‑150 (MQ=255)
                                                                          cAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGa                                                                             <  2:2582032/150‑1 (MQ=255)
                                                                            ccccGAACGCTAAAAGCCCCTGCGGTTGCGTTTCTTCCTTTCTTATATAATCTTTTTTTCTTGTGCCGTATAAACTAGCCGGCAAATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAAt                                                                           <  1:367944/149‑1 (MQ=255)
                                                                              ccGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAg                                                                         >  2:253604/1‑150 (MQ=255)
                                                                              ccGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAg                                                                         >  1:3852687/1‑150 (MQ=255)
                                                                                gAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAgcc                                                                       <  2:2062678/150‑1 (MQ=255)
                                                                                    gcgAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCa                                                                   >  1:2239359/1‑150 (MQ=255)
                                                                                         aaGCACCTGGGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGa                                                              <  2:710790/150‑1 (MQ=255)
                                                                                          cgCACGTGGGGTTGCGGTTCTGCCTTTAGTATCTAATGTGTTGTTCTTGTGCCGGATAAACTATCGGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGACGCCAGCCGaa                                                             <  2:1033688/149‑1 (MQ=255)
                                                                                           gCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAAt                                                            <  1:2261793/150‑1 (MQ=255)
                                                                                            cACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATc                                                           >  2:3521619/1‑150 (MQ=255)
                                                                                            cACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATc                                                           <  2:1851854/150‑1 (MQ=255)
                                                                                               cTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCCAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAAt                                                        >  1:2632486/1‑150 (MQ=255)
                                                                                                  cggttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGc                                                     >  1:296357/1‑150 (MQ=255)
                                                                                                     ttgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTc                                                  >  2:3868351/1‑150 (MQ=255)
                                                                                                      tgcgtttCTTCATTTAGTATCTAATCTTTTGTTCATGTGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCa                                                 <  2:2675266/150‑1 (MQ=255)
                                                                                                      tgcggttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCGGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAACCGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCa                                                 <  2:1194352/150‑1 (MQ=255)
                                                                                                          gttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGAc                                             >  1:96769/1‑150 (MQ=255)
                                                                                                          gttCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGAc                                             >  1:2675816/1‑150 (MQ=255)
                                                                                                           ttcttcATTTAGTATCTAATCTGTTGTTCTTGTGCCGTATAAACTATCCGGAACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGAcc                                            <  1:632344/150‑1 (MQ=255)
                                                                                                            tcttcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCa                                           >  2:747168/1‑150 (MQ=255)
                                                                                                               tcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCAAGCCGAATCAATAGCGACCCGACCCTGc                                        >  2:3903142/1‑150 (MQ=255)
                                                                                                               tcCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTACTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGc                                        >  1:1211555/1‑150 (MQ=255)
                                                                                                                 cTTTTGTATCTAATCTGTTGTTCCTGGGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGccc                                      <  1:3715578/150‑1 (MQ=255)
                                                                                                                  tttAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCca                                     >  1:3121896/1‑150 (MQ=255)
                                                                                                                    tAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCcatc                                   <  1:176388/150‑1 (MQ=255)
                                                                                                                     aGTATCTAATCTGTTGTTCATGTGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCcatca                                  <  1:2182034/150‑1 (MQ=255)
                                                                                                                       tATCTAATGTGTTGTTCATGTGCCGTATAAAATATCGGGCACATCTTCTCTATTCAACGCCCATTCTCATCAAGAGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATa                                <  1:3690294/150‑1 (MQ=255)
                                                                                                                        aTCTAATCTGTTGTTCATGTGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATAc                               <  1:226110/150‑1 (MQ=255)
                                                                                                                         tCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACt                              >  1:3476874/1‑150 (MQ=255)
                                                                                                                         tCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCAACATACt                              >  2:3590674/1‑150 (MQ=255)
                                                                                                                         tCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCAAATCATAACTCTTCAACGCCCAATCACATACAGCGCAAACGTCGGTAGCTTAAGATGACAAAGAATAGCCGCCACCCGAAACAAAAGCGACACGAACATGCCCAACAAAca                              >  1:2463389/1‑149 (MQ=255)
                                                                                                                           tAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTgg                            >  2:132778/1‑150 (MQ=255)
                                                                                                                           tAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTgg                            >  1:1046231/1‑150 (MQ=255)
                                                                                                                            aaTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGc                           >  2:527413/1‑150 (MQ=255)
                                                                                                                            aaTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGc                           >  2:1526191/1‑150 (MQ=255)
                                                                                                                             aTCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCt                          >  2:2961383/1‑150 (MQ=255)
                                                                                                                              tCTGTTGTTCATGTGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTg                         <  1:1484459/150‑1 (MQ=255)
                                                                                                                                tgttgtTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGtt                       >  1:2348913/1‑150 (MQ=255)
                                                                                                                                tgttgtTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGtt                       >  1:2582764/1‑150 (MQ=255)
                                                                                                                                    gttCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCa                   <  1:1556430/150‑1 (MQ=255)
                                                                                                                                     ttCTTGTGCCGTATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAg                  <  2:1536130/150‑1 (MQ=255)
                                                                                                                                       cATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTg                <  2:2207016/150‑1 (MQ=255)
                                                                                                                                           tgCCGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTAc            >  1:3059224/1‑150 (MQ=255)
                                                                                                                                           tgCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTAc            >  1:1994828/1‑150 (MQ=255)
                                                                                                                                           tgCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTAc            >  1:3422354/1‑150 (MQ=255)
                                                                                                                                             ccGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCCACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg          >  1:935209/1‑150 (MQ=255)
                                                                                                                                             ccGGATAGTTTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg          >  1:3573280/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg          >  1:2989216/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg          >  2:3034527/1‑150 (MQ=255)
                                                                                                                                             ccGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACgg          >  1:3264616/1‑150 (MQ=255)
                                                                                                                                               ggATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGACTCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaa        >  2:1923890/1‑150 (MQ=255)
                                                                                                                                               ggATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGaa        <  2:2277459/150‑1 (MQ=255)
                                                                                                                                                  tAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAt     >  2:571107/1‑150 (MQ=255)
                                                                                                                                                     aCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAtgtg  <  2:3849279/150‑1 (MQ=255)
                                                                                                                                                     aCTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAAtgtg  >  1:728316/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGAGCCTGCAATATCTGGTCGGCGGTTCCGTTGATTATACCGAAGGTCTGGAAGGTTCTCGTTTCATCGTGACCAACCCGAACGCGAAAAGCACCTGCGGTTGCGGTTCTTCCTTTAGTATCTAATCTGTTGTTCATGTGCCGGATAAACTATCCGGCACATCTTCTCTCTTCAACGCCCATTCTCATCCAGCGCAAACGTCGGTAGCTTAAGATGCCAACGAATAGCCGCCAGCCGAATCAATAGCGTCACGACCATGCCCATCATACTGGCTGTTTCCAGTGGTACGGAAAATGTG  >  NC_000913/176829‑177127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: