Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,441 C→A 8.0% D437E (GAC→GAA gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4410CA 8.0% 874.5 / 7.8 271D437E (GAC→GAAgtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (122/115);  new base A (30/3);  total (153/118)
Fisher's exact test for biased strand distribution p-value = 7.95e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTT  >  NC_000913/2470292‑2470589
                                                                                                                                                     |                                                                                                                                                    
tttGGAATATCTCAATGTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                                                                                                                      <  1:3396036/150‑1 (MQ=255)
  tGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                                    <  2:2559379/150‑1 (MQ=255)
         tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                             <  2:1058762/150‑1 (MQ=255)
           tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGATATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGt                                                                                                                                           <  1:1085448/150‑1 (MQ=255)
           tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACACAATCGATGt                                                                                                                                           >  2:2375162/1‑150 (MQ=255)
           tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAAAAAAAAGCGAAGt                                                                                                                                           >  1:1255255/1‑150 (MQ=255)
              aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGACAAAATAGAAGTaaa                                                                                                                                        >  2:3439006/1‑150 (MQ=255)
               ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                                       <  1:3146486/150‑1 (MQ=255)
                   aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                                   <  1:598974/150‑1 (MQ=255)
                     gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                                 <  1:3517991/150‑1 (MQ=255)
                     gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                                 <  1:2560121/150‑1 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGAGATGTAAAGCACTAAGt                                                                                                                               >  2:961314/1‑150 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAAAAGAGATTTAAAGCACTAAGt                                                                                                                               >  2:2125412/1‑150 (MQ=255)
                            ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTAATTATAAAAAAAATAAAAGAAATAAAGAGAATAGGGATGTGAAGGACTTAGtgttaa                                                                                                                          >  1:737735/1‑145 (MQ=255)
                             tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAATTAAAAAAAAAAAAAAAAAAATCAATTTAAAACAATAAGTTTAAAt                                                                                                                         >  1:2668880/1‑150 (MQ=255)
                              ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                        <  2:1362276/150‑1 (MQ=255)
                                  gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAATAAATACAAAAGCGATGTAAAGCACTAAGTTTAAAtttagc                                                                                                                    >  1:418805/1‑146 (MQ=255)
                                  gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCGTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATAAATCATTAAAAAAACGAGGTACAGCACTAAGTTTAAAATgcgc                                                                                                                    >  2:2705369/1‑150 (MQ=255)
                                    gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                                  <  2:2445039/150‑1 (MQ=255)
                                       ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGAGATTTAAAGCACTAAGTTTAAATTTCGCGCCaa                                                                                                               >  2:3143145/1‑150 (MQ=255)
                                          tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                            >  2:2284163/1‑150 (MQ=255)
                                           ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATGTAAATCACTAAGTTTAAATTGAGCGCCAATCAt                                                                                                           >  2:2831807/1‑150 (MQ=255)
                                            taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                          <  1:419449/150‑1 (MQ=255)
                                              gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAATTACAATAAAAAAAGAAAAAAACGATGTAAAGCACTAAGTTTTAATTTGGCGCCCATCCTggg                                                                                                        >  1:3245494/1‑149 (MQ=255)
                                                    tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaa                                                                                                  <  2:1018904/150‑2 (MQ=255)
                                                      agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAATAAATAAAGACAAAATAGATGTTAAACACAAATTTTTAATTTCGAGCCAAACATGGCGCGCACaa                                                                                                >  1:2833669/1‑150 (MQ=255)
                                                       gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAATAATTAAAAGAAATAAATAAAATAGAGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAg                                                                                               >  2:1286052/1‑150 (MQ=255)
                                                       gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTAAAAAATAAATAAAAGAAAAAAAGAAAAAAACCATTTAAATCACAAAGTTTAAATTTCGCGCCCATAATGTCCCGCACAAg                                                                                               >  1:3880560/1‑150 (MQ=255)
                                                        caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                              <  2:1318407/150‑1 (MQ=255)
                                                          agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                            <  2:3389067/150‑1 (MQ=255)
                                                          agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                            <  2:3897352/150‑1 (MQ=255)
                                                              tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTGAAATAAATAAATTCAATAGGGATGTAAAGGAATTATTTTTATTTTCGGGCCAATCATTGCGCGCACAATCTttaat                                                                                        >  2:1584164/1‑150 (MQ=255)
                                                              tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAGAAAAAATAGAAGTAAACAACAAAGTTTAAATTTAGCGCCCAACATTGCGCGCACAAGcaaaaaa                                                                                        >  1:153307/1‑144 (MQ=255)
                                                                 ttctGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATACAATAGCGATGTAAATCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACCAGCAATAAAAcc                                                                                     >  2:3885975/4‑150 (MQ=255)
                                                                  tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGAAAAAATCCATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAAGCAAAAATAcaa                                                                                    >  2:1921258/1‑148 (MQ=255)
                                                                    tGGAATGTATTTTTCGTTCTCCCTTGGCTAATACATAACAGCCTTTTTTTTAATTAAAAAAATAATTCAAAGAAATCAAGAAAATAGCTTTGTAAAGCACTAATTTTAAATTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAAc                                                                                  <  1:3704326/150‑1 (MQ=255)
                                                                         tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                             <  2:1852157/150‑1 (MQ=255)
                                                                           tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACGAAGTTTAAATTGCGCGCCAATCATGGCGCGCCCAACCTATAATAACAACCGAAgtt                                                                           >  2:2757214/1‑150 (MQ=255)
                                                                              ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                        <  2:3704354/150‑1 (MQ=255)
                                                                               tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAAAAAAAAAAAAGCGATGTAAACCAATAACTTTAAAATGCGCGCCAATCAAGGCGCGCACAAGCTATAAAAACAAACAAATTtctac                                                                       >  2:464648/1‑148 (MQ=255)
                                                                                 tcgttcgACATTTGCTTATACATAACTGTCGTTTTTTTAATTTATAAAATAATTCAAATAAAAAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctc                                                                     <  1:551364/150‑1 (MQ=255)
                                                                                   gttcgACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                                   <  1:2164489/150‑1 (MQ=255)
                                                                                    ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTAAAAAAAAAATCAAAAAAAACAAGAAAAAAACCAAGTAAAGCACAAACTTTAAAATGCGCGCCAATCCAGGCCCCCACAAACCAAAATAACAACCAAAAATatcctcctc                                                                  >  2:375713/1‑150 (MQ=255)
                                                                                     tcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                                 <  1:2950875/150‑1 (MQ=255)
                                                                                           tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                           <  1:3564384/150‑1 (MQ=255)
                                                                                              gctaaaaCATAACAGTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGAAAATTGCTATTTAAAGCACTAAGTTTAAATTTCGCGCAAATCATGCCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                        <  1:1711822/144‑1 (MQ=255)
                                                                                              gCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                        <  1:2382810/150‑1 (MQ=255)
                                                                                               cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAAAAGCGATGTAAAGCAATAAGATTAAATTTAGCGCCAATCATGGCGCGCACAAGCTATAAAAACAAACAAAATTCACCTCCTCAAAGAGTGAca                                                       >  2:2070571/1‑149 (MQ=255)
                                                                                                ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCCCAAGCTATAATACCAACCAAATTTCTCCTCCTATTAGAGGGACta                                                      >  2:2928672/1‑150 (MQ=255)
                                                                                                   taCATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATATAGATGTAAATCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATAGCAACCAAATTTCTCCTCCTCTTAGTGTGTCGatat                                                   >  2:2834570/1‑150 (MQ=255)
                                                                                                     cATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                                 <  2:1820766/150‑1 (MQ=255)
                                                                                                      aTAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAAGAAAAAAAGAAAAAAGCGATGTAAATCACAAAGGTTAAAATTCGCGCCAAAAATGGCGCGCCCAAGCAAAAATACCAAACAAATATATACACCTCTTAGAGTGACtaaaaatc                                                >  2:1503497/1‑144 (MQ=255)
                                                                                                        aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                              <  1:1501715/150‑1 (MQ=255)
                                                                                                            gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                          >  2:1952412/1‑150 (MQ=255)
                                                                                                            gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                          <  1:1914386/150‑1 (MQ=255)
                                                                                                            gTCGTATTTTTAATTTAAAAAAAAAATAAAATAAAACAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGAGCCCCAATCATGGCGCGCACAACCTAAAAAACCAACCAAAAATCTCCTCAACTAAGAGTGACTATATCTCCAGATa                                          >  2:3640999/1‑150 (MQ=255)
                                                                                                             tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                         >  2:1087793/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                      >  1:1780931/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                      >  1:134016/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                      >  1:2123506/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                      >  2:738549/1‑150 (MQ=255)
                                                                                                                  tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAGTCATGGCGCGCACAAGCTGTAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATGTCTCCTGATAGAATTg                                    >  1:681500/1‑150 (MQ=255)
                                                                                                                  tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTg                                    >  2:2145175/1‑150 (MQ=255)
                                                                                                                   ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                   <  2:3399730/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTACTTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                  <  2:1710693/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                  <  1:2284216/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                  >  2:578368/1‑150 (MQ=255)
                                                                                                                     ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                 <  1:3474460/150‑1 (MQ=255)
                                                                                                                       aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                               >  2:2902101/1‑150 (MQ=255)
                                                                                                                        aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                              >  2:198210/1‑150 (MQ=255)
                                                                                                                         tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                             >  2:1237055/1‑150 (MQ=255)
                                                                                                                         tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                             <  2:3901851/150‑1 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                           <  1:654759/150‑1 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                           >  1:2285920/1‑150 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                           >  1:285654/1‑150 (MQ=255)
                                                                                                                           tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                           <  2:828172/150‑1 (MQ=255)
                                                                                                                              aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                        >  1:3817910/1‑150 (MQ=255)
                                                                                                                              aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                        >  1:1459762/1‑150 (MQ=255)
                                                                                                                              aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa                        >  1:3138944/1‑150 (MQ=255)
                                                                                                                                aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                      <  1:1187538/150‑1 (MQ=255)
                                                                                                                                aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                      <  2:3377071/150‑1 (MQ=255)
                                                                                                                                   aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                   <  1:3204608/150‑1 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                >  2:3358844/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc              >  2:1552880/1‑150 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc              <  2:3500516/150‑1 (MQ=255)
                                                                                                                                        aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc              >  1:2505685/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt             >  1:3320433/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt             >  1:3280909/1‑150 (MQ=255)
                                                                                                                                           gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa           >  2:502500/1‑150 (MQ=255)
                                                                                                                                            aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt          >  1:1141419/1‑150 (MQ=255)
                                                                                                                                            aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt          <  2:2990891/150‑1 (MQ=255)
                                                                                                                                             aaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAtt         <  1:2754379/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCTAAGTAAAGCACTAAGATTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt     <  1:10395/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTATAATACCCACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt     >  2:3769086/1‑150 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt     <  2:3832563/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt     >  1:3116348/1‑150 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt     <  2:3256572/150‑1 (MQ=255)
                                                                                                                                                   gACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgt   >  1:218566/1‑150 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCTCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACGATATCTCATGATAGAATTGAGGTATTTACTATCAAATGCCCTGATTCgttgtt  >  1:1640757/1‑150 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt  <  2:3237270/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTT  >  NC_000913/2470292‑2470589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: