Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 6.3% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 6.3% 489.1 / 13.2 176intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (87/73);  new base T (2/9);  total (91/85)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269994‑3270291
                                                                                                                                                     |                                                                                                                                                    
taaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGACGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc                                                                                                                                                      <  1:3323658/149‑1 (MQ=25)
caaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCCTTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGc                                                                                                                                                      <  1:2282661/149‑1 (MQ=25)
     tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                 <  2:784680/150‑1 (MQ=37)
       aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTgg                                                                                                                                               <  1:973279/150‑1 (MQ=37)
         cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCAATCATCATTCAGCCCCACCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACAGACCCAAAAGCCGCATTCTTTTGGGTATAGCGTCGTGGAc                                                                                                                                             >  1:3778164/1‑150 (MQ=11)
          gATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                            <  1:1688124/150‑1 (MQ=255)
           aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGCGTCGTGGAAAg                                                                                                                                           >  2:1067956/1‑150 (MQ=11)
            tAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGACATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                                                                                                                          <  2:3796556/150‑1 (MQ=255)
             aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                                                                                                                         <  1:1059775/150‑1 (MQ=37)
                ccgatatctttTTGTTATAGTGTCGTGGACAGACAAACATCTTACTCCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGATATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                      <  2:3385219/144‑1 (MQ=11)
                 cgatttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattc                                                                                                                                     <  2:2507745/147‑1 (MQ=37)
                 cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCATAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattc                                                                                                                                     <  2:3625763/150‑1 (MQ=255)
                     ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt                                                                                                                                 <  1:1150406/150‑1 (MQ=37)
                        ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAAAATCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGtcaatcaaaatta                                                                                                                              >  2:3826617/1‑144 (MQ=11)
                         tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                                                                                                             <  2:2993940/150‑1 (MQ=37)
                             ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                                                                                         <  1:1444538/150‑1 (MQ=37)
                                 taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                                                                     <  2:192602/150‑1 (MQ=37)
                                 ctgtgtCGGCGACACTGACTCTTCTTTCGGGCACTCCACAATCACAAACCCGCCGGATCGGGTTTTTACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                                                                     <  1:929074/148‑1 (MQ=11)
                                    tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGACAGTCATTCATATTTATGCCCCTCCaaa                                                                                                                  >  1:3196710/1‑150 (MQ=255)
                                      tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAg                                                                                                                >  1:1083485/1‑150 (MQ=37)
                                       cGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAACCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGc                                                                                                               <  1:1682041/150‑1 (MQ=255)
                                                tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAACCAAAAACccg                                                                                                      >  1:1453212/1‑150 (MQ=255)
                                                  tttcttcTTTCTGCCCCTCAAAAAGAAAAAACCCGCCGAAGGGGTTTTTTACTTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACATTCATTCATCTTTCTGCCCCTCAAAAAGCAAAAACccgcc                                                                                                    <  1:3100709/149‑1 (MQ=11)
                                                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                    >  1:2556978/1‑150 (MQ=37)
                                                   ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                   >  2:2814539/1‑150 (MQ=37)
                                                        cTTCCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                              <  1:1274765/150‑1 (MQ=255)
                                                             tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGGAGCGCGACTTGGCAAGGAATGCATGTTTAGGCCCCCCCAAACCCCAAAACCCCCCGCAGCGGGgtt                                                                                         >  2:154024/1‑150 (MQ=2)
                                                              gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGTTTTTTTTCGTAAACCAGGTGAATCTGACCGATATGCCGATTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                        <  1:1111235/150‑1 (MQ=11)
                                                              gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGtgtt                                                                                        >  2:1867797/1‑150 (MQ=255)
                                                               ccccccCACAACAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGCTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                       <  2:2579920/150‑1 (MQ=11)
                                                               ccccTCCACACGTAAACACCCGCCGAAGCGGGTTATTACGTACACCAGGTGCACCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                       <  2:3602709/150‑1 (MQ=11)
                                                               ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                       >  1:472491/1‑150 (MQ=37)
                                                               ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                       >  2:2972108/1‑150 (MQ=37)
                                                                cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      >  2:479037/1‑150 (MQ=37)
                                                                cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTa                                                                                      >  1:1709549/1‑150 (MQ=255)
                                                                cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      >  1:2962490/1‑150 (MQ=37)
                                                                 ccTCCAAATGCAAATACCCGCCGATGCGTTTTTTTACTTAAAACAGTTTAAACTGACCGATAAGCCGCTTTCTTTTGGTTTTAGCGTCGTGTACTGTCATTCATCTTTCTGCCCCTCAAAAAGCAAAAACCCGCTTCGGCGGGtttttgc                                                                                     <  1:58018/150‑3 (MQ=11)
                                                                 ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTAc                                                                                     >  1:1905800/1‑150 (MQ=37)
                                                                  cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                    >  2:282841/1‑150 (MQ=37)
                                                                   tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTGTTACgg                                                                                   >  2:3553153/1‑149 (MQ=255)
                                                                     cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGAGGGTTTTTACGTaa                                                                                 >  2:39569/1‑150 (MQ=37)
                                                                     cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTaa                                                                                 >  2:2088714/1‑150 (MQ=255)
                                                                       aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                               >  2:310131/1‑150 (MQ=38)
                                                                          tcttaaaCCCGCCGTATCGGGTTATTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                            <  2:972831/146‑1 (MQ=11)
                                                                          gtaaaaaCCCGCTTCGGCGGGTTGTTACGTCAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGCGTCGGGGCCCGTCCTGCATCTGTCTGCCCCGCCCACAGCCCACACCCGCCGCCGCGGGTGGTTACGGaaaaccg                                                                            >  2:2901254/3‑146 (MQ=2)
                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  1:1323951/1‑150 (MQ=37)
                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                          >  2:1052011/1‑150 (MQ=37)
                                                                             aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTg                                                                         >  1:3508199/1‑150 (MQ=37)
                                                                              aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                                                                        <  1:810268/150‑1 (MQ=37)
                                                                               aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa                                                                       >  2:2315376/1‑150 (MQ=37)
                                                                                aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAATCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                      >  1:522277/1‑150 (MQ=255)
                                                                                 cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc                                                                     >  2:710719/1‑150 (MQ=37)
                                                                                  ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTCCGTAAATCAGGTGAAACt                                                                    >  2:644813/1‑150 (MQ=37)
                                                                                  ccgccgAAGCGGGTTATTACGTAAAACAGCTGAAACTGACCGATAACCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                    <  1:1808770/150‑1 (MQ=11)
                                                                                   cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                            <  2:36977/77‑1 (MQ=39)
                                                                                        aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTGAAACTGAACGa                                                              >  1:2006333/1‑150 (MQ=255)
                                                                                            gggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAATCAGGTGAAACTGACCGATAAg                                                          >  1:3629557/1‑150 (MQ=37)
                                                                                                  ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGCGTCGTGGACAGTCCTTCATCTTTCTGCCCCGCCAAAAGCCAAAACCCGCCGAAGCGGGTTTGTACGGAAATCAGGTGAAACTGACCGATAAGCCGGGt                                                    >  2:1750890/1‑150 (MQ=14)
                                                                                                    aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTc                                                  >  1:663917/1‑150 (MQ=255)
                                                                                                          aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  2:2556978/150‑1 (MQ=255)
                                                                                                          aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  2:814562/150‑1 (MQ=255)
                                                                                                           ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg                                           >  1:2951304/1‑150 (MQ=255)
                                                                                                            cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAAACATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTGTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                          >  1:1861728/1‑150 (MQ=255)
                                                                                                            cAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                          >  1:3937446/1‑150 (MQ=255)
                                                                                                               gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                       >  2:2264717/1‑150 (MQ=255)
                                                                                                                tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTATGCCCCGCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAATAAGGGGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAg                                      >  1:1635878/1‑150 (MQ=17)
                                                                                                                 gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                     <  1:2814539/150‑1 (MQ=255)
                                                                                                                  aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                    <  2:1336146/150‑1 (MQ=255)
                                                                                                                   aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                                   >  2:3272447/1‑150 (MQ=255)
                                                                                                                      tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                <  1:74980/150‑1 (MQ=255)
                                                                                                                      tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                <  1:805023/150‑1 (MQ=255)
                                                                                                                      tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTGCGGCCCCACCCAAAGCAAAAACCCGCCGCACCGCGTGGTTACGTAAAGCAGGTGAAACTGACCCAGCAGCCGGGTGCTGGCGAGGACCGtcagtc                                >  2:2036852/1‑150 (MQ=11)
                                                                                                                      tGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                <  1:2281103/150‑1 (MQ=255)
                                                                                                                        aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattcat                              <  1:3271588/150‑1 (MQ=255)
                                                                                                                          cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGACAGTCATTCATCt                            >  2:427366/1‑150 (MQ=255)
                                                                                                                           gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                       >  2:2145371/1‑138 (MQ=255)
                                                                                                                           gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                       <  1:2145371/138‑1 (MQ=255)
                                                                                                                           gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                           <  1:1052011/150‑1 (MQ=255)
                                                                                                                              aagcatctttctttTGTGTTTAGGGTCGTTGAATGTCATTCATCTTTCTGCCCCTCACACAGCAAAAACCAGCCGAAGAGTGTTTTTACGTAAATCAGGTTAAACTGTCCGATATGCCGGTTTCTGTCGTGGACAGTCATTCATCTAGGc                        <  1:848937/144‑1 (MQ=17)
                                                                                                                              aaGCCGCTTTCTTTTGTGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGc                        <  1:64175/150‑1 (MQ=255)
                                                                                                                                gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTTACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:2089270/150‑1 (MQ=255)
                                                                                                                                 ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGAAAATCAGGTGAAAAAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGTCCAg                     >  2:2327431/1‑150 (MQ=255)
                                                                                                                                  cGCTTTCTTTTTGGTATAGCGTCGTGGACAGTCATTAAAATTTATTCCCCACCCAAAACAAAAAAACCCCCAAGCGGGGTTTTACCTAAAAAATGTGAAACAGACCGAAAAACCCGGGTCTTTAGTGGACAGTAATTAATATAAGCCAGc                    >  1:1354267/1‑150 (MQ=255)
                                                                                                                                   gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                                                                             <  1:2058591/92‑1 (MQ=37)
                                                                                                                                   gCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                                                                             >  2:2058591/1‑92 (MQ=18)
                                                                                                                                    ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                  >  2:396615/1‑150 (MQ=255)
                                                                                                                                      ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                                                                                         <  2:2687448/45‑1 (MQ=38)
                                                                                                                                       tctttTGGGTATAGCGTCGTGGACAGTCATTAATCATTCTGCCCCTCCAAAAGCAAAAAACCGCCGAATCGGGTTTTTAAGTAAATAAGGTTAAACTTACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAAATAGGCCAGCAATCg               >  1:1994784/1‑150 (MQ=18)
                                                                                                                                        ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCGGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc              <  1:2901254/150‑1 (MQ=255)
                                                                                                                                          tttGGGTATAGCGTCGTGGTCAGTCATTCATCTTTCTTCCCCTCCAAATGCAAAAACCCGCCGAAGCGGGTTTTTTGGTAAATCAGGTTAAACTGACCGTTAAGCCGGGTTGTGTGGTGGACAGTGATTCATCTAGGCCAGCAATCGCtg            >  2:699308/1‑149 (MQ=18)
                                                                                                                                          tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTACCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc            >  2:734159/1‑150 (MQ=255)
                                                                                                                                           ttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGACACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa           <  1:1069100/150‑1 (MQ=255)
                                                                                                                                           ttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa           <  2:1851475/150‑1 (MQ=255)
                                                                                                                                              ggTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTg        <  1:1605999/150‑1 (MQ=255)
                                                                                                                                               gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg       <  1:1556550/150‑1 (MQ=255)
                                                                                                                                               gTATAGCGTCGTGGACAGTAATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAACGCTCACTgg       >  1:3808643/1‑150 (MQ=255)
                                                                                                                                                 ataGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCt     >  1:2917167/1‑150 (MQ=255)
                                                                                                                                                  taGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc    >  2:1527535/1‑150 (MQ=255)
                                                                                                                                                  taGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc    <  2:2773085/150‑1 (MQ=255)
                                                                                                                                                    gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa  <  1:115534/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269994‑3270291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: