Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,515 T→A 7.2% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5150TA 7.2% 640.4 / 15.5 213intergenic (+70/+17)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (68/113);  new base A (15/4);  total (94/119)
Fisher's exact test for biased strand distribution p-value = 9.18e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCT  >  NC_000913/3959367‑3959664
                                                                                                                                                    |                                                                                                                                                     
cAGCCATGAGATTGAGCAGGTAGGTAAGAAACTGCGCGGATATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAATGCAGTCTCCTg                                                                                                                                                      <  2:3104696/150‑1 (MQ=38)
 aGCCATGCGATTGAGCAGGTATGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGTGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                                                                     <  2:2132560/150‑1 (MQ=255)
  gCCATGCGATTGAGCAGGGAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                                                                                    <  2:3713613/150‑1 (MQ=255)
   ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgag                                                                                                                                                   <  2:193132/150‑1 (MQ=255)
     aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                                                                 <  1:402123/150‑1 (MQ=255)
     aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                                                                 <  2:2627740/150‑1 (MQ=255)
      tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCTCACCCCCAACCTCAACCACAGGGAGAGGGAGAAAAAACACAATGCATTCGCCTGGAGAAg                                                                                                                                                >  2:1601221/1‑150 (MQ=255)
       gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                                                                               <  2:2576224/150‑1 (MQ=255)
             gAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTga                                                                                                                                         <  2:1687866/150‑2 (MQ=255)
              aGCAGGGAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCt                                                                                                                                        <  1:3694605/150‑1 (MQ=255)
               gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTACCACAGGGAGGGGGAGAAAACACACAAGGCCGTATCCAGGAGAAGGCCGTGCTa                                                                                                                                       >  1:3208908/1‑150 (MQ=38)
                  ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                                    <  2:424840/150‑1 (MQ=255)
                     aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                                                                                                                                 <  1:128213/150‑1 (MQ=255)
                     aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGtt                                                                                                                                 <  1:290280/150‑1 (MQ=255)
                     aggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCTCACCCACAGGGACAGGGAGAAAAAACACAAGGCCATCTACAGGAGAAGGCCTGGCTATTAGtt                                                                                                                                 >  2:3524831/1‑150 (MQ=255)
                       ctaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGAACCGGGATAACATAGCAAAGAGGCCCACACCCCCACCCACTCCCACAGGGAGAGGGAGAAAACACACAAGGCCATCTCCAGGCGGAGGCCGTGCTATTAGTTGc                                                                                                                               >  2:3448095/2‑150 (MQ=255)
                        taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                                                              <  1:1986308/150‑1 (MQ=255)
                        taAGAAACGGCGCGGCTATATGACAGAGATGATACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCg                                                                                                                              <  2:3910555/150‑1 (MQ=255)
                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                                             <  2:3547153/150‑1 (MQ=255)
                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                                                                             <  1:2281247/150‑1 (MQ=255)
                         aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACACGGGGAGGGAGAAAACACACAAGGCCATCTACTGGCGAAGGCCTTGCGATTAGTTTCgg                                                                                                                             >  1:2595890/1‑150 (MQ=255)
                           gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACACCCACAGGGAGAGGGAGAAAAAAAACAAGGCCGTATCCAGGAGAAGGCCGTGCGATTATTTTCGGTa                                                                                                                           >  1:505454/1‑150 (MQ=255)
                           gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCCACCCCCCCCCACACCCCCACGGCGGGGGGGAGAACAAACAACGCCATGACCGGGGGACTGCCGGGCGATTAGATGCggga                                                                                                                           >  1:1688872/1‑148 (MQ=255)
                            aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                                          <  1:670347/150‑1 (MQ=255)
                            aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCAACCACAGGGAGAGGGAGAAAACACACAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                                          >  2:506719/1‑150 (MQ=255)
                                 gcgcGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCGCACCCCCAGGGAGAGGGAGAAAACCATCCAGGCCGTCTCCGGGAGAAGGGCTTGCGAGTAGTTGCGGTACAGCAc                                                                                                                     >  2:2399268/1‑150 (MQ=37)
                                     ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCCACCCACACCCACAGGGAGAGGGAGAAAACACACAAGGCCTTCTCCGGGAGAAGGCCGTGCTATTAGTTTCGGTACAGCACCATa                                                                                                                 >  2:3193735/1‑150 (MQ=38)
                                      gCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTaa                                                                                                                <  1:2727637/150‑1 (MQ=255)
                                          tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat                                                                                                            <  2:3094396/150‑1 (MQ=255)
                                            tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACACCACCATAATGAtgt                                                                                                          >  2:2420630/1‑150 (MQ=255)
                                                aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACATTAATGATGTGata                                                                                                      <  2:884204/150‑1 (MQ=255)
                                                       aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACCGCACCTTAATGATGTGATATCCGAAc                                                                                               >  2:2945070/1‑150 (MQ=255)
                                                       aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACACCCACAGGGAGAGGGAGAAAACACACAACGCCATCTCCAGGAGAAGGCCGTGCGAATATTTGAGGTACAGCACCATAATGATTTGATATACGAAc                                                                                               >  2:2045320/1‑150 (MQ=255)
                                                          cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                            <  1:1117157/150‑1 (MQ=255)
                                                          cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                            <  2:1275961/150‑1 (MQ=255)
                                                                 cTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa                                                                                     <  2:278602/150‑1 (MQ=255)
                                                                 cTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACTCCCACACGGAGGGGGAGAAAAAACACAAAGCCATATACGGGAGAAAGCCATTCTAATATTTTCGGTACAGCACCATAAATATTTTATATACCAACAGGGTGTGcc                                                                                     >  2:1421921/1‑149 (MQ=255)
                                                                   gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc                                                                                   <  2:30752/150‑1 (MQ=255)
                                                                    ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                  >  2:3612674/1‑150 (MQ=255)
                                                                    ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                  <  1:3362910/150‑1 (MQ=255)
                                                                    ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                  <  1:3327351/150‑1 (MQ=255)
                                                                    ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                                  <  1:2428101/150‑1 (MQ=255)
                                                                       cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                               <  1:45757/150‑1 (MQ=255)
                                                                       cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                               <  2:109754/150‑1 (MQ=255)
                                                                       cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                               <  1:2798161/150‑1 (MQ=255)
                                                                        gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc                                                                              <  2:362392/150‑1 (MQ=255)
                                                                         ggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCg                                                                             <  1:384742/150‑1 (MQ=255)
                                                                            tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                                                                          <  2:1648485/150‑1 (MQ=255)
                                                                             aaGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCg                                                                         <  2:2745388/150‑1 (MQ=255)
                                                                              aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                        <  1:2545791/150‑1 (MQ=255)
                                                                              aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                                        <  1:2796591/150‑1 (MQ=255)
                                                                                tGCGCGCTGATGCCCTCACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTATCCTGGAGAAGGCCGTGCGATTTGTTGCGGTACCGCACCTTAATGCTGTGATATCCGAACTGGGTGTGCAGCGGGCCGggcggcg                                                                      >  2:2060273/1‑149 (MQ=37)
                                                                                 gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                                     <  1:1595910/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:1431814/150‑1 (MQ=255)
                                                                                   gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                                   <  1:328303/150‑1 (MQ=255)
                                                                                      cTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTa                                                                <  2:2658054/150‑1 (MQ=255)
                                                                                            cccTCACCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAc                                                          <  2:1511290/150‑1 (MQ=255)
                                                                                            cccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGAc                                                          >  2:2487163/1‑150 (MQ=255)
                                                                                                  ccccGACCCACACCCACAGGGAGAGGGAGAAAAAACAAAAGGCCTTCAACAGGAGAATGCCAAGATAATAGTTTCGGTACAGCACCAAAATGATGTGATATCCCAAAAGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAACGGACAAcaga                                                    >  1:1527979/1‑150 (MQ=255)
                                                                                                    ccGACCCTCACCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                                                  >  1:3045371/1‑150 (MQ=255)
                                                                                                     cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGAACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                                 >  2:2370141/1‑150 (MQ=255)
                                                                                                        cccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                              >  2:1258647/1‑150 (MQ=255)
                                                                                                        cccTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCa                                              <  1:1007728/150‑1 (MQ=255)
                                                                                                         ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGat                                                                                                       >  2:3181339/1‑92 (MQ=255)
                                                                                                          ctctCCCACTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTCGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                                            <  2:3612803/150‑1 (MQ=255)
                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTCATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                                           >  2:869569/1‑150 (MQ=255)
                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                                           >  1:2645798/1‑150 (MQ=255)
                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                                           >  2:1855208/1‑150 (MQ=255)
                                                                                                           tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                                           >  1:3457992/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGCCGGCCTTGCTATTAGTTGCGGTACAGCACCTTCATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTCCCGGACCAGAGAAAACCACTTTATc                                       >  1:1349721/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                       >  2:3659350/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                       >  1:1562383/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                       >  1:236842/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                       >  2:2457382/1‑150 (MQ=255)
                                                                                                               ccACAGGGAGAGGGAGAAAACACTCAAGGCCTACTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                                       >  1:3835426/1‑150 (MQ=255)
                                                                                                                 acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa                                     >  2:1538698/1‑150 (MQ=255)
                                                                                                                  caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTTTGCAGCGGGCCGGTCGGCTCCAGTACCGGAAAAGAGAAAACCACTTTATCGaa                                    <  2:3315896/150‑1 (MQ=255)
                                                                                                                  caGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa                                    >  2:326073/1‑150 (MQ=255)
                                                                                                                           ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc                           >  2:637268/1‑150 (MQ=255)
                                                                                                                           ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc                           >  2:448240/1‑150 (MQ=255)
                                                                                                                           ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc                           >  2:2490496/1‑150 (MQ=255)
                                                                                                                           ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTGTATCGAACGCCGGAAc                           >  1:3095079/1‑150 (MQ=255)
                                                                                                                            gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGTACAAGAGAAAACCACTTTATCGAACGCCGGAAcc                          <  1:2035698/150‑1 (MQ=255)
                                                                                                                            gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAcc                          >  2:777748/1‑150 (MQ=255)
                                                                                                                              gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGTTATCCTAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt                        <  1:2053860/150‑1 (MQ=255)
                                                                                                                              gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGCCAACCACTTTCTCGAACGCCGGACCCAt                        >  2:3157027/1‑150 (MQ=255)
                                                                                                                               aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATc                       >  1:3576127/1‑150 (MQ=255)
                                                                                                                                aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt                      <  1:3543973/150‑1 (MQ=255)
                                                                                                                                aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt                      <  1:2521720/150‑1 (MQ=255)
                                                                                                                                aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt                      >  2:116626/1‑150 (MQ=255)
                                                                                                                                aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt                      <  1:987252/150‑1 (MQ=255)
                                                                                                                                 taatagcAAGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg                     >  2:172983/7‑150 (MQ=255)
                                                                                                                                      tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt                >  1:1999540/1‑150 (MQ=255)
                                                                                                                                        aaGGCCTTCTCCAGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTgg              >  2:2973510/1‑150 (MQ=255)
                                                                                                                                         aGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                            <  1:2339616/71‑1 (MQ=255)
                                                                                                                                         aGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                                            >  2:2339616/1‑71 (MQ=255)
                                                                                                                                          ggCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCg            <  1:208955/150‑1 (MQ=255)
                                                                                                                                            ccTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGa          <  1:116626/150‑1 (MQ=255)
                                                                                                                                                  ccTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAc    <  2:692118/150‑1 (MQ=255)
                                                                                                                                                  ccTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAc    <  1:627226/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCT  >  NC_000913/3959367‑3959664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: