Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,181,279 G→T 11.9% R12L (CGT→CTT)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,181,2790GT11.9% 924.1 / 91.6 278R12L (CGT→CTT) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (129/116);  new base T (17/16);  total (146/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

Only 100 of 281 total aligned reads displayed.

TAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGCT  >  NC_000913/4181133‑4181428
                                                                                                                                                  |                                                                                                                                                     
 aGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAg                                                                                                                                                   >  1:2329332/1‑150 (MQ=255)
     aCAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACGGAGAAAAAACGTTTTCGTAAGGAtt                                                                                                                                               >  1:3153925/1‑150 (MQ=255)
     aCAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGAtt                                                                                                                                               >  2:2122224/1‑150 (MQ=255)
       aGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAAGAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGAtttt                                                                                                                                             <  1:867822/150‑1 (MQ=255)
        ggCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTg                                                                                                                                            <  1:2565835/150‑1 (MQ=255)
         gCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTgg                                                                                                                                           <  2:594157/150‑1 (MQ=255)
             tGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCGATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTaaa                                                                                                                                       <  2:1076876/150‑1 (MQ=255)
             tGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTaaa                                                                                                                                       <  1:1834483/150‑1 (MQ=255)
               cGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACg                                                                                                                                     >  1:663820/1‑150 (MQ=255)
                 gAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCACTGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGTc                                                                                                                                   <  2:351434/150‑1 (MQ=255)
                 gAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGTc                                                                                                                                   >  2:2248752/1‑150 (MQ=255)
                      tgtTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACaa                                                                                                                              <  1:2813418/150‑1 (MQ=255)
                      tgtACCATTTTCCGGACAACAAAATAGTGTTGCACAAACTGTCCGCTCACTGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACaa                                                                                                                              <  1:2008322/150‑1 (MQ=255)
                        ttCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGt                                                                                                                            >  2:360658/1‑150 (MQ=255)
                        ttCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGt                                                                                                                            >  2:3289716/1‑150 (MQ=255)
                           cATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCt                                                                                                                         <  1:1027478/150‑1 (MQ=255)
                           cATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCt                                                                                                                         <  2:3714071/150‑1 (MQ=255)
                             ttttCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTgg                                                                                                                       <  1:998800/150‑1 (MQ=255)
                                 ccGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCACTGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGt                                                                                                                   <  2:1799718/150‑1 (MQ=255)
                                 ccGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGt                                                                                                                   <  2:3286173/150‑1 (MQ=255)
                                  cGGTCAACAAAATAGTGTTGCACACACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTa                                                                                                                  <  1:1883494/150‑1 (MQ=255)
                                    gTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTAcc                                                                                                                >  1:3936261/1‑150 (MQ=255)
                                    gTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTAcc                                                                                                                >  1:616250/1‑150 (MQ=255)
                                    gTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTAcc                                                                                                                >  1:2056096/1‑150 (MQ=255)
                                    gTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTAcc                                                                                                                >  2:2417210/1‑150 (MQ=255)
                                       aacaaAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTa                                                                                                             >  2:1630228/1‑150 (MQ=255)
                                        acaaAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTAt                                                                                                            <  1:2495637/150‑1 (MQ=255)
                                        acaaAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTAt                                                                                                            <  2:2056096/150‑1 (MQ=255)
                                        acaaAAGCGTGTTGCACAAACTGTCCGCTCACTGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTAt                                                                                                            <  2:2667780/150‑1 (MQ=255)
                                         caaAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTAtc                                                                                                           >  2:3825173/1‑150 (MQ=255)
                                            aaTAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTcc                                                                                                        >  1:3675744/1‑150 (MQ=255)
                                               aGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCttt                                                                                                     >  2:1995022/1‑150 (MQ=255)
                                                 tgtTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGACAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGTATGTACCTTATCTCCTTTCt                                                                                                   <  2:3067256/150‑1 (MQ=255)
                                                    tGCACAAACTGTCCGCTCATTTGACAGATGGGTCGACTCTTCAGCGAGCTGCGCTACCATATGGTTTACACCTATACCGAGAAAAAACGTTGTCGTTAGGATTTTGGTAAACGTCCAAAAGTTCTGGATGTACCTTATCTCCTTTCTATc                                                                                                <  1:2997312/150‑1 (MQ=255)
                                                    tGCACAAACTATCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATc                                                                                                <  2:2308404/150‑1 (MQ=255)
                                                     gCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATcc                                                                                               >  1:3618540/1‑150 (MQ=255)
                                                      cacaAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTcc                                                                                                                                  <  2:786116/114‑1 (MQ=255)
                                                      cacaAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTcc                                                                                                                                  >  1:786116/1‑114 (MQ=255)
                                                       acaAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAg                                                                                             >  1:1625451/1‑150 (MQ=255)
                                                       acaAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAg                                                                                             <  1:46438/150‑1 (MQ=255)
                                                       acaAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAg                                                                                             >  2:2539886/1‑150 (MQ=255)
                                                           aCTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTg                                                                                         <  1:3757753/150‑1 (MQ=255)
                                                            cTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGa                                                                                        <  2:902809/150‑1 (MQ=255)
                                                            cTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGa                                                                                        <  1:2230633/150‑1 (MQ=255)
                                                             tGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGAc                                                                                       >  2:535036/1‑150 (MQ=255)
                                                                 cGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGt                                                                                   >  2:2646139/1‑150 (MQ=255)
                                                                  gCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGtt                                                                                  <  2:2760591/150‑1 (MQ=255)
                                                                   cTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGttt                                                                                 >  1:3293497/1‑150 (MQ=255)
                                                                     cAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCa                                                                               <  1:3630572/150‑1 (MQ=255)
                                                                         ggACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGaaa                                                                           >  2:2950195/1‑150 (MQ=255)
                                                                           aCAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAAtt                                                                         >  2:327527/1‑150 (MQ=255)
                                                                            cAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAAttt                                                                        <  2:3472864/150‑1 (MQ=255)
                                                                               aTGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATc                                                                     <  1:3260983/150‑1 (MQ=255)
                                                                                 gggTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGACCACAAGTTCTGGATGTACCTTATCTCAAAACTATCCAGCTTGACTCGTTTCAGAAATTTATCGa                                                                   >  2:1672446/1‑150 (MQ=255)
                                                                                  ggTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAg                                                                  >  1:524901/1‑150 (MQ=255)
                                                                                   gTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGc                                                                 <  1:2980068/150‑1 (MQ=255)
                                                                                        cTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGAt                                                            <  1:3825173/150‑1 (MQ=255)
                                                                                         ttGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATc                                                           >  1:2817877/1‑150 (MQ=255)
                                                                                         ttGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATc                                                           >  1:1683405/1‑150 (MQ=255)
                                                                                          tGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATcc                                                          >  2:3127752/1‑150 (MQ=255)
                                                                                            tCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTg                                                        >  1:389169/1‑150 (MQ=255)
                                                                                            tCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTg                                                        >  1:2019555/1‑150 (MQ=255)
                                                                                            tCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTg                                                        >  1:344042/1‑150 (MQ=255)
                                                                                              aGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGaa                                                      >  2:2691675/1‑150 (MQ=255)
                                                                                              aGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGaa                                                      >  2:3808885/1‑150 (MQ=255)
                                                                                              aGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGaa                                                      >  2:3568597/1‑150 (MQ=255)
                                                                                                cGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAgg                                                    <  1:1970145/150‑1 (MQ=255)
                                                                                                cGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAgg                                                    <  1:3704721/150‑1 (MQ=255)
                                                                                                 gAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAggg                                                   <  2:1833260/150‑1 (MQ=255)
                                                                                                      gAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTa                                              <  2:3906350/150‑1 (MQ=255)
                                                                                                        ggAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATg                                            >  2:3373685/1‑150 (MQ=255)
                                                                                                         gAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATgg                                           >  1:2218718/1‑150 (MQ=255)
                                                                                                            cccTATGGTTGACTCCTAGACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGGTCGGGATGGACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCt                                        <  1:608923/150‑1 (MQ=255)
                                                                                                               tATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGa                                     >  1:1549109/1‑150 (MQ=255)
                                                                                                                 tGGTTTACTCCTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAg                                   <  2:471215/150‑1 (MQ=255)
                                                                                                                 tGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAg                                   >  1:1118413/1‑150 (MQ=255)
                                                                                                                   gTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAgct                                 <  2:2858664/150‑1 (MQ=255)
                                                                                                                   gTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAgct                                 <  1:1156696/150‑1 (MQ=255)
                                                                                                                    tttACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAgctg                                <  2:215836/150‑1 (MQ=255)
                                                                                                                       aCTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCtt                             <  1:2667766/150‑1 (MQ=255)
                                                                                                                          ccTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttcc                          <  2:2344689/150‑1 (MQ=255)
                                                                                                                          ccTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttcc                          <  1:1776957/150‑1 (MQ=255)
                                                                                                                          ccTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttcc                          <  2:1139840/150‑1 (MQ=255)
                                                                                                                           cTATACCGAGAAAAAACGTATTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttccg                         >  1:2463952/1‑150 (MQ=255)
                                                                                                                           cTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttccg                         >  1:2283594/1‑150 (MQ=255)
                                                                                                                            tataCCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTttccgt                        >  2:110912/1‑150 (MQ=255)
                                                                                                                                 cGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCttt                            >  1:1438567/1‑141 (MQ=255)
                                                                                                                                 cGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCttt                            <  2:1438567/141‑1 (MQ=255)
                                                                                                                                 cGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTtccgttccgt                   >  1:3580409/1‑150 (MQ=255)
                                                                                                                                  gagaAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTa                  >  2:3235987/1‑150 (MQ=255)
                                                                                                                                    gaAAAAACGTATTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTAtt                >  2:1623505/1‑150 (MQ=255)
                                                                                                                                            gTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTc        <  1:1869061/150‑1 (MQ=255)
                                                                                                                                            gTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTc        <  1:3650168/150‑1 (MQ=255)
                                                                                                                                             tATTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCa       <  1:2122224/150‑1 (MQ=255)
                                                                                                                                              aTTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCag      >  2:1930534/1‑150 (MQ=255)
                                                                                                                                              aTTCTTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCag      >  2:1951386/1‑150 (MQ=255)
                                                                                                                                              aTTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCag      >  2:1753777/1‑150 (MQ=255)
                                                                                                                                               ttCGTCAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGATTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCaga     <  1:2638872/150‑1 (MQ=255)
                                                                                                                                                 cGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGc   <  1:1298141/150‑1 (MQ=255)
                                                                                                                                                  gTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGCt  >  1:327670/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTGTTGCACAAACTGTCCGCTCAATGGACAGATGGGTCGACTTGTCAGCGAGCTGAGGAACCCTATGGTTTACTCCTATACCGAGAAAAAACGTATTCGTAAGGATTTTGGTAAACGTCCACAAGTTCTGGATGTACCTTATCTCCTTTCTATCCAGCTTGACTCGTTTCAGAAATTTATCGAGCAAGATCCTGAAGGGCAGTATGGTCTGGAAGCTGCTTTCCGTTCCGTATTCCCGATTCAGAGCT  >  NC_000913/4181133‑4181428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: