Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,554 G→T 13.5% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5540GT13.5% 1514.0 / 83.0 488intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (62/349);  new base T (72/5);  total (134/354)
Fisher's exact test for biased strand distribution p-value = 3.79e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 494 total aligned reads displayed.

TATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107406‑107703
                                                                                                                                                      |                                                                                                                                                     
tatGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGa                                                                                                                                                      <  1:5593023/150‑1 (MQ=255)
 atGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGaa                                                                                                                                                     <  1:10940889/150‑1 (MQ=255)
  tgtgACCTTCCAGGACGACGCCGAACTGCCGTTGGCCTTCAAAGCACCTTCAGCGGTACTGGCATACCGACCTTTATACTGTCGTATAAATTTCGCCTGGCATATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAt                                                                                                                                                    <  2:10974116/150‑1 (MQ=255)
   gtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtt                                                                                                                                                   <  2:3504812/150‑1 (MQ=255)
   gtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtt                                                                                                                                                   <  1:1951870/150‑1 (MQ=255)
    tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTAttt                                                                                                                                                  >  2:7489048/1‑150 (MQ=255)
    tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTAttt                                                                                                                                                  >  1:1380892/1‑150 (MQ=255)
     gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtttt                                                                                                                                                 <  2:2957695/150‑1 (MQ=255)
     gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtttt                                                                                                                                                 <  1:7529745/150‑1 (MQ=255)
     gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTGAAattt                                                                                                                                                 >  2:9705323/1‑150 (MQ=255)
      cccTTCCAGGACGACGCCGAACTGCCGTTGGCCTTCAATGCGCCTTCAGCTTTACTGGCATAACTACATTTATACTGCCTTTTAAAATTCGACTGGCAAATCTGGAACTCTCTCCGGCATGGTGAACCCGTCG‑T‑TTTTTTTTGAATTTta                                                                                                                                                <  2:10650552/149‑1 (MQ=255)
        cTTCCATGACGTCGCATAACTGCCGTTGGCCTTCCAAGCGCCTTCAGCTGTACTGGCATAACGCCATTTATACTGTCGTATAAAATTCGCCCGGCAAATCTGGCACCCACTCCGGCCAGGTTCACCATTCGTT‑TTTTTTTTGAATTTtat                                                                                                                                               <  2:3738677/150‑1 (MQ=255)
         ttCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATaa                                                                                                                                             <  1:3758397/150‑1 (MQ=255)
           ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGTATTTTATAaga                                                                                                                                           >  2:832821/1‑150 (MQ=255)
           ccAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAaga                                                                                                                                           <  1:5939696/150‑1 (MQ=255)
            cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAagag                                                                                                                                          <  1:2762416/150‑1 (MQ=255)
             aGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGc                                                                                                                                         <  1:10045865/150‑1 (MQ=255)
                acgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCta                                                                                                                                       <  2:3234207/150‑1 (MQ=255)
                     gCAGAACAGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCCTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑A‑TTTTTATTTTAATTTATAAAATATAaaaaaa                                                                                                                                 >  2:6003247/1‑150 (MQ=255)
                            tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTTAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                                                                                          <  1:10816833/150‑1 (MQ=255)
                            tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTAATTTTATAAGAGCTATAAAAAACGGTGc                                                                                                                          <  1:9522191/150‑1 (MQ=255)
                            tGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCAAAACGACATTTATACTGTCGTATAAAAATCGACTGGCAAATCTGGCACTCTCTCCGGCCACGTGAACCAGTCG‑T‑GTTTTTTTTTATTTTTTAATAAGTATaaaaaaaaaata                                                                                                                          >  1:4905644/1‑144 (MQ=255)
                                gTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAAc                                                                                                                      <  1:5320663/150‑1 (MQ=255)
                                  tGGCCTTCAAAGCGCCTTCCGCTTTACTGGCATAACTAAATTTAAACTGTCTTATAAAATTCGACTGGAAAATCTGGCACTCTCCCCGGCCAGTTTAACCAGTGT‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGc                                                                                                                    <  2:8120488/150‑1 (MQ=255)
                                   ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt                                                                                                                   <  2:8517075/150‑1 (MQ=255)
                                    gCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTAAATTTATAATAGCTAAAAAAAAACGTGCGaaaccgg                                                                                                                  >  2:7585808/1‑145 (MQ=255)
                                    gCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTGATTTTTTAAAAGAGATAAAAAAACCTGCGCACGccg                                                                                                                  >  1:7154007/1‑148 (MQ=255)
                                     ccTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTAATTTATAAAATATATAAAAAAAGGGTCGAACGCAGt                                                                                                                 >  2:7866834/1‑150 (MQ=255)
                                      cTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTTTTTTTTTAATATATAAAAAAAAAAGTGCGGACGCAGtt                                                                                                                >  1:3493510/1‑150 (MQ=255)
                                         cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                                                                                             <  1:7246050/150‑1 (MQ=255)
                                         cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                                                                                             <  1:6703333/150‑1 (MQ=255)
                                          aacgcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  2:4024330/147‑1 (MQ=255)
                                          aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  2:876479/150‑1 (MQ=255)
                                          aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  1:8832895/150‑1 (MQ=255)
                                          aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                                                                                            <  2:9572270/150‑1 (MQ=255)
                                           acgcgcCTTCCGCGGTACTGGCAGACCGACAGTTATCCTCGCGGATAAAAGTCGACTGGCAACCCGGGCCCTCGCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTGGATAAGAGCTAGAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  2:3614141/148‑1 (MQ=255)
                                           aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  1:1810715/150‑1 (MQ=255)
                                           aaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCtt                                                                                                           <  2:7421656/150‑1 (MQ=255)
                                            aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACGGTCGTATAAAATTCGACTGGCAAATCTGGCACGCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                                                                                          <  2:9380168/150‑1 (MQ=255)
                                             gcgcCTTCAGCTGTACGGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:4252073/150‑1 (MQ=255)
                                             gcgcCGTCAGCTGTACCGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCCGGCACTCGCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                                                                                         <  1:6952984/150‑1 (MQ=255)
                                                 cTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                                                                                     <  2:683440/150‑1 (MQ=255)
                                                   tCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTAATTTTATAAATTAGATAAAAAAAAGTGCGAAAGCTGTTTTGGTAATCAcat                                                                                                   >  2:4687506/1‑148 (MQ=255)
                                                           aCTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                                                                                           <  1:15555/150‑1 (MQ=255)
                                                               gCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGTATTTTTTAATATCTATAAAAAAAGGTGCGAACGCGGGTTTTTTAAGCACATTTACGCACAACt                                                                                       >  2:9216469/1‑150 (MQ=255)
                                                                 aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGGATTTTATAAAAGCTAAAAAAAAAAGTTCGAACCCAGTTTTATTAATCACATTTACGCACCACATa                                                                                     >  2:1568115/1‑150 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTTAATTTATAAGAGCGATAAAAAAAGGTGCGAACGCTGTTTTGTTTATCACATTTGCGCACAACtaaa                                                                                    >  2:1782522/1‑147 (MQ=255)
                                                                  tAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt                                                                                    <  1:4160000/150‑1 (MQ=255)
                                                                     cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCtt                                                                                 <  1:3377304/150‑1 (MQ=255)
                                                                        cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa                                                                               <  2:268496/150‑1 (MQ=255)
                                                                        cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCAt                                                                              <  1:5766252/150‑1 (MQ=255)
                                                                           ttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg                                                                           <  1:6550665/150‑1 (MQ=255)
                                                                            tataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGt                                                                          <  1:11078984/150‑1 (MQ=255)
                                                                             ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  1:3990071/150‑1 (MQ=255)
                                                                              taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGc                                                                        <  2:10279220/150‑1 (MQ=255)
                                                                                 tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  2:6293954/150‑1 (MQ=255)
                                                                                 tGTCGTATAAAATTAGACTGGCATCTCTTGCTCTCTCTCCGGCCAGTTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt                                                                     <  2:6522168/150‑1 (MQ=255)
                                                                                   tCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg                                                                   <  2:7017305/150‑1 (MQ=255)
                                                                                    cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGa                                                                  <  2:1017543/150‑1 (MQ=255)
                                                                                      tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACACTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACt                                                                <  1:119820/150‑1 (MQ=255)
                                                                                         aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTTTATTTTTTAA‑ATCAATAAAAAAAATTGGGGACCCAGTTTTATTTATCACATTTGCGCACAAAATAAATTGATTGGTGCTGGGGTCTggg                                                             >  2:4871606/1‑148 (MQ=255)
                                                                                         aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  1:3582622/150‑1 (MQ=255)
                                                                                               cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCttt                                                       <  1:2499862/150‑1 (MQ=255)
                                                                                                 aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:9105636/150‑1 (MQ=255)
                                                                                                 aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:1705388/150‑1 (MQ=255)
                                                                                                 aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTAAATTTATAAAAGCGAAAAAAAAAGGTTCGAAACCGTGTTTGTTAAGCACAATTAACCACAAAATATCATCAATAGTGCTGTGGACGGCAGGCTTTaa                                                     >  2:5595605/1‑150 (MQ=255)
                                                                                                   tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATg                                                   <  1:8255761/150‑1 (MQ=255)
                                                                                                    ggCAAAGCTGGCACTCTCGCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTGGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGa                                                  <  2:8017690/150‑1 (MQ=255)
                                                                                                     gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGAt                                                 <  2:1084396/150‑1 (MQ=255)
                                                                                                     gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGATTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGAt                                                 <  2:7271349/150‑1 (MQ=255)
                                                                                                      cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATa                                                <  1:9454769/150‑1 (MQ=255)
                                                                                                      cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATa                                                <  2:5430615/150‑1 (MQ=255)
                                                                                                      cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATa                                                <  1:9216469/150‑1 (MQ=255)
                                                                                                       aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATaa                                               <  1:7866834/150‑1 (MQ=255)
                                                                                                             ggCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtg                                         <  1:1444743/150‑1 (MQ=255)
                                                                                                              gCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:8861336/150‑1 (MQ=255)
                                                                                                                aCTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgc                                      <  1:7414640/150‑1 (MQ=255)
                                                                                                                  tctctcCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  1:3176375/150‑1 (MQ=255)
                                                                                                                   cactcCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  2:2098595/148‑1 (MQ=255)
                                                                                                                     ctcCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTTATTTTATAATAGCTAAAAAAAAAGGTGGGAAAGCCGTTTTCTTTAGCACATTTTCCCCCAACAAATATTAAATAGGTGGGTGGTCTGCAGGCGTTAATTGTAAGATTTTTGGGCGaa                                 >  2:567317/1‑150 (MQ=255)
                                                                                                                           ccAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGt                           <  1:9866874/150‑1 (MQ=255)
                                                                                                                            cagtttaaCCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTTCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  2:5274046/144‑1 (MQ=255)
                                                                                                                            cAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  1:1035764/150‑1 (MQ=255)
                                                                                                                             aGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  1:8509880/150‑1 (MQ=255)
                                                                                                                             aGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  1:6309601/150‑1 (MQ=255)
                                                                                                                               ttcaaCCAGTCT‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGa                       <  1:6111912/147‑1 (MQ=255)
                                                                                                                                       gTCT‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATc               <  1:7686988/150‑1 (MQ=255)
                                                                                                                                          g‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCggg            <  2:9143105/150‑1 (MQ=255)
                                                                                                                                            t‑ttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           <  2:4411615/150‑1 (MQ=255)
                                                                                                                                            t‑ttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           >  1:4728385/1‑150 (MQ=255)
                                                                                                                                               tttttttGACTTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTGTCCGCACAACTTCTCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGAGCGGGATg         <  2:1221442/150‑1 (MQ=255)
                                                                                                                                               tttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATg         >  1:460610/1‑150 (MQ=255)
                                                                                                                                                ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  1:4507799/150‑1 (MQ=255)
                                                                                                                                                ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  1:10690426/150‑1 (MQ=255)
                                                                                                                                                ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  2:9894393/150‑1 (MQ=255)
                                                                                                                                                   tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCaa     >  1:2213330/1‑150 (MQ=255)
                                                                                                                                                    ttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAAt    >  1:2989317/1‑150 (MQ=255)
                                                                                                                                                     tGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATa   >  2:2418603/1‑150 (MQ=255)
                                                                                                                                                     tGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATa   >  2:4984839/1‑150 (MQ=255)
                                                                                                                                                      gAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATaa  <  1:3088650/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107406‑107703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑