Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,005,308 A→T 5.2% A149A (GCA→GCT pldA → outer membrane phospholipase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,005,3080AT 5.2% 2497.8 / 11.3 797A149A (GCA→GCTpldAouter membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base A (376/349);  new base T (4/66);  total (380/417)
Fisher's exact test for biased strand distribution p-value = 1.81e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 810 total aligned reads displayed.

GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457
                                                                                                                                                     |                                                                                                                                                     
 ggATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAg                                                                                                                                                      >  1:8362106/1‑150 (MQ=255)
   aTTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGt                                                                                                                                                    <  2:9226367/150‑1 (MQ=255)
    ttttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGtt                                                                                                                                                   <  1:4107827/150‑1 (MQ=255)
    ttttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGtt                                                                                                                                                   <  1:7782341/150‑1 (MQ=255)
    ttttAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGtt                                                                                                                                                   <  1:10715292/150‑1 (MQ=255)
       tAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGa                                                                                                                                                <  2:10888873/150‑1 (MQ=255)
           cccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCt                                                                                                                                            >  2:2437329/1‑150 (MQ=255)
           cccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCt                                                                                                                                            >  1:3455999/1‑150 (MQ=255)
            ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTg                                                                                                                                           >  1:11150065/1‑150 (MQ=255)
            ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTg                                                                                                                                           >  2:3401968/1‑150 (MQ=255)
            ccGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTg                                                                                                                                           >  2:10705310/1‑150 (MQ=255)
              gAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTgcg                                                                                                                                         <  2:9296765/150‑1 (MQ=255)
                  tCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAt                                                                                                                                     >  2:5100326/1‑150 (MQ=255)
                   cGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                                                                                                                                    <  1:6343234/150‑1 (MQ=255)
                   cGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                                                                                                                                    <  1:2244442/150‑1 (MQ=255)
                   cGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtg                                                                                                                                    >  1:8079732/1‑150 (MQ=255)
                    ggTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgt                                                                                                                                   <  1:6690536/150‑1 (MQ=255)
                     gtgtTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGAtgtg                                                                                                                                  <  1:6144771/150‑1 (MQ=255)
                            gTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgg                                                                                                                           <  1:3771476/150‑1 (MQ=255)
                             tGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATggg                                                                                                                          <  2:488030/150‑1 (MQ=255)
                              gCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                                                                                                                         <  2:3188638/150‑1 (MQ=255)
                              gCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                                                                                                                         >  1:3954411/1‑150 (MQ=255)
                              gCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                                                                                                                         <  2:876767/150‑1 (MQ=255)
                               cGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGt                                                                                                                        >  2:2772306/1‑150 (MQ=255)
                               cGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGt                                                                                                                        <  1:5743257/150‑1 (MQ=255)
                                gTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGta                                                                                                                       >  1:5456284/1‑150 (MQ=255)
                                    tataCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAAc                                                                                                                   <  1:989180/150‑1 (MQ=255)
                                     ataCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAAcc                                                                                                                  >  2:1968750/1‑150 (MQ=255)
                                     ataCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCATGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAAcc                                                                                                                  >  1:2063412/1‑150 (MQ=255)
                                       aCGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCGTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCAc                                                                                                                <  2:3886837/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTTGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGAAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGTTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              <  1:5671532/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTTCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              <  2:10299285/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              <  2:4554672/150‑1 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              >  2:7361350/1‑150 (MQ=255)
                                         gCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACCACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGa                                                                                                              >  1:4496910/1‑150 (MQ=255)
                                           aaaaaTCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGAct                                                                                                            >  2:7673551/1‑150 (MQ=255)
                                           aaaaaTCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGAct                                                                                                            <  2:10087520/150‑1 (MQ=255)
                                                  cTGGTGCCAACTGTCCAATAGCTAAGAGTATTCACCTTTTCGTGAAACCAACTACCAACCCAAATTGTTCCTCGGTTTTGCCACCTATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGTAGATGGGGTATAACCACGACTCTAACgg                                                                                                     <  2:5213661/150‑1 (MQ=255)
                                                     gtggCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCg                                                                                                  >  1:1683192/1‑150 (MQ=255)
                                                        gCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTc                                                                                               >  1:9532249/1‑150 (MQ=255)
                                                             tGTCCAATAGCGAAGAGTCTTCACGGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTccgacc                                                                                          <  2:9628227/150‑1 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     >  1:6894625/1‑150 (MQ=255)
                                                                  aaTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGGGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCcgacccgacc                                                                                     >  1:10510741/1‑150 (MQ=255)
                                                                   aTAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCt                                                                                    >  2:3050359/1‑150 (MQ=255)
                                                                    tAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTc                                                                                   >  1:7320339/1‑150 (MQ=255)
                                                                     cgCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGTTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTcc                                                                                  <  2:6019317/149‑1 (MQ=255)
                                                                      gCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTccc                                                                                 <  2:6931562/150‑1 (MQ=255)
                                                                        gAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGc                                                                               >  2:2261629/1‑150 (MQ=255)
                                                                         aaGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCa                                                                              <  2:6471147/150‑1 (MQ=255)
                                                                            agTCTTCACCTTTTCGTGAAACCAACTCCGAACCGAATTTGTTCCTGGGTTTTGCCACCTATTACCGTTTTGCTGTTTGGACGCTGCGCTATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCt                                                                           <  1:3066634/150‑1 (MQ=255)
                                                                                ttCACCGTTTGGTGAAACCAACTACGAACCGAAATTGTTCCTCGTTTTTGCCACCGATTACCTTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       <  2:8407421/150‑1 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       >  1:1180074/1‑150 (MQ=255)
                                                                                ttCACCGTTTCGTGAAACCAACTACCAACCCAAATTGTACCTCGGTTTTGCCACCGATTACCGATTTGCAGGTTGGACGCTGCGCGATGTGGACATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGaa                                                                       <  2:819109/150‑1 (MQ=255)
                                                                                  cACCTTTTCTTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGAGGCTGCGCGATGTGGAGTTGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAAcc                                                                     <  1:7152484/150‑1 (MQ=255)
                                                                                      gTTTCTTGAAACCAACTACGAACCGCAATTGTTCCTCGTTTTTGCCACCGTTTACCGTTTTGCCGGTTGGACGCTGCGCGTTGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCt                                                                 <  1:4233878/150‑1 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            >  1:6517338/1‑150 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            <  1:7581970/150‑1 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            <  2:7214249/150‑1 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            >  1:1009170/1‑150 (MQ=255)
                                                                                           gTGAAACCAACTACGAACCGCAATTGTTCATCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTtata                                                            >  1:1950356/1‑150 (MQ=255)
                                                                                                aCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGc                                                       <  1:9275459/150‑1 (MQ=255)
                                                                                                aCCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGc                                                       <  2:4470371/150‑1 (MQ=255)
                                                                                                   aaCTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTg                                                    >  1:5280810/1‑150 (MQ=255)
                                                                                                    aCTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGa                                                   <  2:5405207/150‑1 (MQ=255)
                                                                                                     cTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCTGGTTGGACGCTGCGCGTTGTGGATATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGAt                                                  <  1:2464127/150‑1 (MQ=255)
                                                                                                       aCGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATgg                                                >  2:9821201/1‑150 (MQ=255)
                                                                                                        ccaaCCGCATTTTTTCCTCGTTTTTGCCACCGATTACCGTTTTGCAGTTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGc                                               <  2:1107145/148‑1 (MQ=255)
                                                                                                         gAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATgggg                                                                                                                         <  1:1493849/75‑1 (MQ=255)
                                                                                                           aCCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGa                                            >  2:10426505/1‑150 (MQ=255)
                                                                                                             cGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGaaa                                          >  2:9413134/1‑150 (MQ=255)
                                                                                                               cAATTGTTCCTCGGTTTTGCCACCTATTACCGTTTTGCTGGTTGGACGCTGCGCTATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAAc                                        <  2:1182596/150‑1 (MQ=255)
                                                                                                                aaTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACg                                       <  2:5820367/150‑1 (MQ=255)
                                                                                                                   tGTTCCTCGGTTTTGCCACCTATTACCGTTTTGCAGGTTGGACGCTGCGCGTTGTGGAGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTa                                    <  2:4257628/150‑1 (MQ=255)
                                                                                                                   tGTTCCTCGGTTTTGCCACCGTTTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTa                                    <  2:9101080/150‑1 (MQ=255)
                                                                                                                    gTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                   >  2:1626040/1‑150 (MQ=255)
                                                                                                                    gTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                   <  2:3538285/150‑1 (MQ=255)
                                                                                                                         tCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggc                              >  1:4894364/1‑150 (MQ=255)
                                                                                                                         tCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggc                              >  2:7164714/1‑150 (MQ=255)
                                                                                                                          cGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAActggct                             <  2:1055985/150‑1 (MQ=255)
                                                                                                                              tttGCCACCGATTACCGTTTTGCTGGTTGGACGCGGCGCGATGTGGAGCTGGGGTATACCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                         <  1:8824511/150‑1 (MQ=255)
                                                                                                                                 gCCACCGATTACGGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGaa                      <  1:7477903/150‑1 (MQ=255)
                                                                                                                                  ccACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAg                     >  2:6277979/1‑150 (MQ=255)
                                                                                                                                   cACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGt                    >  1:10368342/1‑150 (MQ=255)
                                                                                                                                    aCCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTg                   >  1:5096057/1‑150 (MQ=255)
                                                                                                                                      cGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGaa                 <  1:5928427/150‑1 (MQ=255)
                                                                                                                                       gATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAg                >  2:5630026/1‑150 (MQ=255)
                                                                                                                                       gATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAg                <  1:8922345/150‑1 (MQ=255)
                                                                                                                                          tACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCg             >  2:2110104/1‑150 (MQ=255)
                                                                                                                                           aCCGTTTTGCTGTTTGGACGCTGCGCGATGTGTAGTTGGTGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGt            <  2:6608296/150‑1 (MQ=255)
                                                                                                                                           aCCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGt            <  1:2647204/150‑1 (MQ=255)
                                                                                                                                            ccGTTTTGCAGGTTGGACGCTGCGCGATGTGGATATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTg           <  2:1420665/150‑1 (MQ=255)
                                                                                                                                            ccGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTg           <  1:10174679/150‑1 (MQ=255)
                                                                                                                                            ccGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTg           <  1:7034137/150‑1 (MQ=255)
                                                                                                                                             cGTTTTGCAGGTTGGCCGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTgg          <  1:3231786/150‑1 (MQ=255)
                                                                                                                                              gTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGt         >  2:8565585/1‑150 (MQ=255)
                                                                                                                                                tttGCAGGTTGGACGCTGCGCGATGTGGAGTTGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAt       <  2:8881313/150‑1 (MQ=255)
                                                                                                                                                  tGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgt     >  1:10264459/1‑150 (MQ=255)
                                                                                                                                                   gCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTAtgtg    <  1:4699207/150‑1 (MQ=255)
                                                                                                                                                    cAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtgg   >  1:8620558/1‑150 (MQ=255)
                                                                                                                                                     aGGTTGGACGCTGCGCGCTGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggt  <  2:7944361/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGGATTTTAGGCCCGAACTCGGTGTTGGGTGCGTCTTATACGCAAAAATCCTGGTGGCAACTGTCCAATAGCGAAGAGTCTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGT  >  NC_000913/4005159‑4005457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: