Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,070,469 A→T 7.2% intergenic (‑68/+46) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,070,4690AT 7.2% 1604.5 / 13.5 525intergenic (‑68/+46)yihR/yihSputative sulphoquinovose mutarotase/sulphoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base A (391/67);  new base T (2/47);  total (402/123)
Fisher's exact test for biased strand distribution p-value = 3.98e-32
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 571 total aligned reads displayed.

AATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATAT  >  NC_000913/4070320‑4070618
                                                                                                                                                         |                                                                                                                                                     
aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGg‑‑a                                                                                                                                                       <  1:3439004/150‑1 (MQ=255)
aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGg‑‑a                                                                                                                                                       >  1:2827505/1‑150 (MQ=255)
 aTCCCCCGCGGCAAGTGAAACGGTCTGTCCCCTGCAATGCATATTGGTGAGTTGCAGACAAGGCACCATAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAACACTGCCCCGGACGGTCCCCTCGCCCCGGGGGGg‑‑ag                                                                                                                                                      >  2:6719523/1‑150 (MQ=255)
 aTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGg‑‑ag                                                                                                                                                      <  2:10005380/150‑1 (MQ=255)
 aTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGg‑‑ag                                                                                                                                                      <  1:4738000/150‑1 (MQ=255)
  tCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGg‑‑aga                                                                                                                                                     <  2:6553082/150‑1 (MQ=255)
   cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑agag                                                                                                                                                    <  2:1899069/150‑1 (MQ=255)
   cccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑agag                                                                                                                                                    <  1:7496343/150‑1 (MQ=255)
     cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCTCCCCAAAGGGG‑‑CGAggg                                                                                                                                                  <  1:9422170/150‑1 (MQ=255)
      ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGt                                                                                                                                                 >  2:2837996/1‑150 (MQ=255)
      ccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGt                                                                                                                                                 >  2:3038130/1‑150 (MQ=255)
        gcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAACACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTa                                                                                                                                               <  2:961277/150‑1 (MQ=255)
         cgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTACCCTCTCCCCAAAGGGG‑‑CGAggggacc                                                                                                                                              <  1:6333476/150‑5 (MQ=255)
           gCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAggg                                                                                                                                            >  1:3923284/1‑150 (MQ=255)
            cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGCCATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGga                                                                                                                                           <  2:9126204/150‑1 (MQ=255)
             aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGG‑‑AGAGGGTTAGGgag                                                                                                                                          >  1:2005691/1‑150 (MQ=255)
              aGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGgaga                                                                                                                                         >  2:7056358/1‑150 (MQ=255)
               gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGagag                                                                                                                                        <  2:3497421/150‑1 (MQ=255)
               gTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGagag                                                                                                                                        >  1:8058342/1‑150 (MQ=255)
                tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAATCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGTgg                                                                                                                                       >  2:9539336/1‑150 (MQ=255)
                tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAgg                                                                                                                                       <  1:2696898/150‑1 (MQ=255)
                tGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTGTGGGG‑‑AGAGGGTTAGGGAGAgg                                                                                                                                       >  2:1029578/1‑150 (MQ=255)
                 gAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAggg                                                                                                                                      >  1:290269/1‑150 (MQ=255)
                  aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAgggg                                                                                                                                     >  2:986161/1‑150 (MQ=255)
                  aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAgggg                                                                                                                                     >  2:5656919/1‑150 (MQ=255)
                  aaaCGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAgggg                                                                                                                                     >  1:10810989/1‑150 (MQ=255)
                   aagggTCTGTCCACTGAAATGAATTTTGGTGTTTTGCATAAACGGCACATTATTTAACGACATACAGCCTCCCTTGTGGCTGATGTAAAACCAAAAAACTGCACGGGACGGTCCCCTCGCCCCTTTGGGT‑‑TGAGGGTTCGGTTGAGGGGa                                                                                                                                    <  2:3205217/147‑1 (MQ=255)
                    ccGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTACTTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGGTGGGG‑‑AGAGGGTTAGGGAGAGGGGaa                                                                                                                                   >  2:445854/2‑150 (MQ=255)
                       gtgtGTCCAGTGAAATGTATTTGGTTTTTTTGCATACATGGCACCTTAATTAACGACATACCGCCCCCCTTGAGGCTGATGTAAAACCCAAACACTGCCCCGGAGGGTCCCCTCGCCCCTTTGGGT‑‑TGTGGGTTAGGTTGTGGGGAAAAc                                                                                                                                <  2:404140/147‑1 (MQ=255)
                          tGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGGTGGGG‑‑AGAGGGGTAGGGAGAGGGGAAAACCCg                                                                                                                             >  2:4971300/1‑150 (MQ=255)
                            tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                           >  2:5292004/1‑150 (MQ=255)
                            tCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCGTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                                                                                           >  2:8203500/1‑150 (MQ=255)
                              cACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCAcc                                                                                                                         >  1:5706467/1‑150 (MQ=255)
                                cTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCt                                                                                                                       >  1:10524786/1‑150 (MQ=255)
                                     aTGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                                                                                  >  2:5497940/1‑150 (MQ=255)
                                        cATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                                                                                               >  2:9498108/1‑150 (MQ=255)
                                         attttGGTTATTTGCATAAAAGGCACATTAATAAACGACATACAGCCCCCCTTGTGGATGATGTAAAACCCAAAAACTCCCCCGGACGGGCCCCTCGCCCCTTTGGGG‑‑AGTGGTTTCGGGAGTGGGGAAAACCCGCACCCTATTTTCAGa                                                                                                              <  2:7378760/147‑1 (MQ=255)
                                          tatTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGT‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                                                                                             <  1:11090886/150‑1 (MQ=255)
                                             tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGACAACCCGCACCCTATTTTCAGATCAg                                                                                                          >  1:8848833/1‑150 (MQ=255)
                                              ggTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGt                                                                                                         >  1:1095600/1‑150 (MQ=255)
                                              ggTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGt                                                                                                         >  1:3612520/1‑150 (MQ=255)
                                               gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt                                                                                                        >  1:10069678/1‑150 (MQ=255)
                                                tGATTTGCATACAAGGCAGCTTAATTAACGACATACAGCCTCCCGTGAGGCTGTTGTCAAACCCAAAAACTGCACCGGTCGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGTAAACCCGCACCCTATTTTCAGTTCAGTTa                                                                                                       >  2:5430807/1‑150 (MQ=39)
                                                 gATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAt                                                                                                      >  1:2400023/1‑150 (MQ=255)
                                                     tcaaTACACGCCACATTAATTAACCACATACAGCCCCCCGTGCGGCTGATTAAAAACCCAAAAACGGCACGGGACGGGCCCCTCCCCCCTTTGGGG‑‑AGTGGGTTTGGGTGCGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCg                                                                                                  <  2:10928990/147‑1 (MQ=255)
                                                       cATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                                                                                                >  2:835516/1‑150 (MQ=255)
                                                        aTACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt                                                                                               >  1:5442196/1‑150 (MQ=255)
                                                          aCAAGCCACTTAAATAAACCACAAACACCCTCCCGTGTGGCTTTTTAAAAACCAAAACACGCCACCGGAGGGCCCCCCCCCCCCTTTGGGG‑‑AGCGGTTTCGGGTGTGGGTAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa                                                                                             <  1:9112405/150‑1 (MQ=255)
                                                                cACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGCCGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAGCAGTTATTTCGCATTAATATcc                                                                                       >  1:1539393/1‑150 (MQ=255)
                                                                     taattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagcag                                                                                  >  2:1021409/1‑150 (MQ=255)
                                                                      aattaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGa                                                                                 >  1:1373496/1‑150 (MQ=255)
                                                                       attaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc                                                                                >  2:6353609/1‑150 (MQ=255)
                                                                        ttaaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCCATTTCCAGATCAGTTATTTCGCATTAATATCCAGCAGAcc                                                                               >  2:8702456/1‑150 (MQ=255)
                                                                         taaCGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAccc                                                                              >  1:4283649/1‑150 (MQ=255)
                                                                            cGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCt                                                                           >  2:1643269/1‑150 (MQ=255)
                                                                             gACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTg                                                                          >  2:5847260/1‑150 (MQ=255)
                                                                               acatcAGCCTCACGGGAGGCTGTATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGc                                                                         >  1:9247976/5‑150 (MQ=39)
                                                                                aTACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCaa                                                                       >  2:6919159/1‑150 (MQ=255)
                                                                                 tACCGCCTCCCGTGTGGCTTATGACAAACCAAAAAACGCCACGGGAGGGCCCCCTCGCCCCTTGGGGG‑‑TGGGGTTTTGGGAGTGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAAc                                                                      <  1:8188047/150‑1 (MQ=255)
                                                                                 tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAAc                                                                      >  2:7736532/1‑150 (MQ=255)
                                                                                 tACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAAc                                                                      >  1:8697176/1‑150 (MQ=255)
                                                                                     gCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCt                                                                  >  1:4415684/1‑150 (MQ=255)
                                                                                      ccTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTGATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTg                                                                 >  2:2172398/1‑150 (MQ=255)
                                                                                          ccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGc                                                             >  2:5090246/1‑150 (MQ=255)
                                                                                          ccGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGc                                                             >  2:6243919/1‑150 (MQ=255)
                                                                                           cGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTCGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGcc                                                            >  1:590538/1‑150 (MQ=255)
                                                                                             tgcggCTTATGACAAACCAAAAACCCCCCCCGGCGGGCCCCCGCGCCCCTTGGGGG‑‑TGTGGTTTTGGGAGCGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCAt                                                          <  1:6879578/147‑1 (MQ=255)
                                                                                                         tCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTa                                               >  1:3241946/1‑150 (MQ=255)
                                                                                                         tCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTa                                               >  1:9697319/1‑150 (MQ=255)
                                                                                                           aaaCCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAAc                                             >  2:1461578/1‑150 (MQ=255)
                                                                                                               ccAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGa                                         >  1:2199859/1‑150 (MQ=255)
                                                                                                                cAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGAt                                        >  1:2990699/1‑150 (MQ=255)
                                                                                                                cAAAAACTGCACCGGACGGTCCCCTCGCCCCTATGGGG‑‑AGAGGGTAAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAACAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGAt                                        >  2:6603948/1‑150 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAGCGGGATa                                       >  1:7049198/1‑150 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGAtc                                       >  2:4636585/1‑149 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa                                       >  1:4940174/1‑150 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa                                       >  1:1039058/1‑150 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa                                       >  1:6077031/1‑150 (MQ=255)
                                                                                                                 aaaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATa                                       >  1:6666483/1‑150 (MQ=255)
                                                                                                                  aaaaCTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATAc                                      >  1:10254358/1‑150 (MQ=255)
                                                                                                                       tGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCggg                                 >  2:1536863/1‑150 (MQ=255)
                                                                                                                       ccccccGGGCGGGCCCCCCCCCCCGTTGGGGG‑‑TGTGGTTGTGGGTGGGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCgg                                  <  1:9847756/146‑1 (MQ=255)
                                                                                                                            cGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAc                            >  2:5988716/1‑150 (MQ=255)
                                                                                                                             ggccGGTCCCCTCGCCCTTTTGGGT‑‑TGTGGGTTTGGTTGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAcc                           <  2:2575005/147‑1 (MQ=255)
                                                                                                                              cggcggccccccGCCCCCTTGGGGGTTGTGGGTTGGGGCGGGGCCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCAc                            <  1:7833990/144‑1 (MQ=255)
                                                                                                                                  taacccTCTCCCCAAAGGGG‑‑CGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCa                      >  2:7556103/4‑150 (MQ=255)
                                                                                                                                   tCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCaa                     >  1:737646/1‑150 (MQ=255)
                                                                                                                                     cccTCTCCCCAAAGGGG‑‑CGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATg                   >  2:10507725/1‑150 (MQ=255)
                                                                                                                                     cccTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATg                   >  1:8466541/1‑150 (MQ=255)
                                                                                                                                       cccGCCCCTGGGGGT‑‑TGTGGGTTTGGGTGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgca                 <  2:3751885/148‑1 (MQ=255)
                                                                                                                                       cTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgca                 >  2:5215951/1‑150 (MQ=255)
                                                                                                                                       cTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgca                 >  2:4257894/1‑150 (MQ=255)
                                                                                                                                         cGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgcagc               >  2:4897889/1‑150 (MQ=255)
                                                                                                                                          gCCCCTTGGGGG‑‑AGTGGGTTTGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATgcagca              <  2:10755894/150‑1 (MQ=255)
                                                                                                                                                tttGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGa         >  2:11080581/1‑150 (MQ=255)
                                                                                                                                                ttggggg‑‑TGTGGTTTGGGGTGGGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGa         <  1:6444829/147‑1 (MQ=255)
                                                                                                                                                     gg‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAat    >  2:8087169/1‑150 (MQ=255)
                                                                                                                                                     gg‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAat    >  2:1148855/1‑150 (MQ=255)
                                                                                                                                                      g‑‑agaGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAata   >  2:7904477/1‑150 (MQ=255)
                                                                                                                                                         agagGGTTAGGGAGAGGGGAAAACCCGCTCCCTATTTTGAGATCAGTTATTTCGCATTAATATCCAGCAGTCGCGCTGCTACCGGTGGAGCCATGGCGGGGGCTATCGGGATACGCGGGATCTCCAGGCAATGGAGCTGGTGATAAttat  >  2:2037248/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
AATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCCAAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGG‑‑AGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCGCTGGAGCCATGCCGGGCGCTAACGGGATACGCGGGATCACCAGGCAATGCAGCAGGTGATAAATAT  >  NC_000913/4070320‑4070618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: