Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,406,1290TC98.6% 3142.9 / 13.2 834intergenic (+144/‑61)purA/nsrRadenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Reads supporting (aligned to +/- strand):  ref base T (3/9);  new base C (380/442);  total (383/451)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 834 total aligned reads displayed.

GTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTC  >  NC_000913/4405982‑4406275
                                                                                                                                                   |                                                                                                                                                  
gTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCtg                                                                                                                                                  >  1:8716354/1‑150 (MQ=255)
    ttCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACAATGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGAGACACAGCCTGTGGc                                                                                                                                              >  2:8983370/1‑150 (MQ=255)
     tCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCt                                                                                                                                             >  1:521308/1‑150 (MQ=255)
     tCTGGTACGCCTGGCAGATAGTTGGCCTGCCGTGCGACCAGTGTGATACATTGCTGTGTCGGGTAAGCGATTACGCTATAAGACACAGTGTTAAATCGTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGTCACTGCCTTTGGCt                                                                                                                                             <  2:6757939/150‑1 (MQ=255)
      cTGGTACGCCTGGCAGATATTTTGCCTGCGGGGCGAACAGTGTTATCCTTTGCTGTTTCGGGTAAGCAATTACGCTATCCTAAACAGTTTTAAATCCTCGCTTTTTTCCTTCCCCTAACTTAATTAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                            <  1:3494009/150‑1 (MQ=255)
      cTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTg                                                                                                                                            <  2:10147503/150‑1 (MQ=255)
       tGGTACGCCTGGCAGATATCTTGCCTGCCGGGCGAACTGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTAGCCGACACAGTGTTAAACCCTCGCTTTTTTCCTTCCCCGAACTTAAATAAATTAGCGACACAGCCTGTGGCTgg                                                                                                                                           <  1:7613725/150‑1 (MQ=255)
         gTACGCCTGGCATATATTTTGCCTGCCGGGCTAACAGTGTTATACATTGCTTTGTTGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGtt                                                                                                                                         <  1:9024195/150‑1 (MQ=255)
         gTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGtt                                                                                                                                         >  1:8221616/1‑150 (MQ=255)
          tACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGttt                                                                                                                                        >  2:10503238/1‑150 (MQ=255)
           aCGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTa                                                                                                                                       <  2:5367601/150‑1 (MQ=255)
            cGCCTGGCAGATATTTTGCCTGCGGGGCTAACAGTGTGATACTTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTTAAATAATTTAGCGACACAGCCTGTGGCTGGTTTat                                                                                                                                      <  1:2319651/150‑1 (MQ=255)
            cGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTat                                                                                                                                      <  2:4543628/150‑1 (MQ=255)
             gCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatc                                                                                                                                     <  2:2729947/150‑1 (MQ=255)
              ccTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatca                                                                                                                                    <  2:8400665/150‑1 (MQ=255)
                tGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcatc                                                                                                                                  >  2:4021417/1‑150 (MQ=255)
                tGGCAGATATTTTGCCTGCCGGGCGAACAGAGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcatc                                                                                                                                  >  1:9543727/1‑150 (MQ=255)
                tGGCAGATATTTTGCCTGCCGAGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTatcatc                                                                                                                                  >  1:999776/1‑150 (MQ=255)
                 ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACTTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                 <  1:4730436/150‑1 (MQ=255)
                 ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTTAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                 <  2:6622776/150‑1 (MQ=255)
                 ggCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTAtcatca                                                                                                                                 <  1:9857486/150‑1 (MQ=255)
                  gCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCGCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCaa                                                                                                                                >  1:1780190/1‑150 (MQ=255)
                       tatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                                           <  2:3398001/150‑1 (MQ=255)
                       tatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaa                                                                                                                           <  1:1979672/150‑1 (MQ=255)
                        atTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATaaa                                                                                                                          <  1:6343009/150‑1 (MQ=255)
                         ttttGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAAt                                                                                                                         <  2:6157019/150‑1 (MQ=255)
                            tgcctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTa                                                                                                                      <  2:2334730/150‑1 (MQ=255)
                             gcctgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAt                                                                                                                     <  2:5727747/150‑1 (MQ=255)
                                tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttt                                                                                                                  >  2:9808283/1‑150 (MQ=255)
                                tgccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttt                                                                                                                  >  1:10530299/1‑150 (MQ=255)
                                 gccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                                 >  1:6803009/1‑150 (MQ=255)
                                 gccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAttttt                                                                                                                 >  2:7606202/1‑150 (MQ=255)
                                  ccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGtttttttt                                                                                                                <  1:2838372/150‑1 (MQ=255)
                                  ccGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTAtttttt                                                                                                                >  1:4426125/1‑150 (MQ=255)
                                   cGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTc                                                                                                               <  1:10617638/150‑1 (MQ=255)
                                   cGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTc                                                                                                               <  2:8856958/150‑1 (MQ=255)
                                       cGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTTTTAAATCCTCGCTTTTTTCCTTCCCCTAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                                                                           <  1:3132628/150‑1 (MQ=255)
                                        gAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAt                                                                                                          >  2:5010370/1‑150 (MQ=255)
                                          aCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGTAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAttt                                                                                                        <  1:4985431/150‑1 (MQ=255)
                                          aCAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAATTTAGCGACACAGCCTGTGGCTGGTTTATCATCAACATAAATGTATTTTTTCCCGAttt                                                                                                        <  1:5501640/150‑1 (MQ=255)
                                           cAGTGTGATACATTGCTGTGTCGGGTAATCCATTACGCTATCCTACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTc                                                                                                       <  2:7271337/150‑1 (MQ=255)
                                              tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCt                                                                                                    >  2:1613866/1‑150 (MQ=255)
                                                 gATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                                                                                 <  1:8578908/150‑1 (MQ=255)
                                                 gATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                                                                                 <  1:3641756/150‑1 (MQ=255)
                                                  aTACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCCTTTg                                                                                                >  1:5969290/1‑150 (MQ=255)
                                                   tACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                                                                               >  2:1069646/1‑150 (MQ=255)
                                                    aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATATATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAg                                                                                              <  2:4368791/150‑1 (MQ=255)
                                                    aCATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAg                                                                                              >  2:6920054/1‑150 (MQ=255)
                                                           tgtgTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtg                                                                                       >  2:7804397/1‑150 (MQ=255)
                                                             tgtCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgtg                                                                                     >  2:4682390/1‑150 (MQ=255)
                                                               tCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCa                                                                                   <  2:923292/150‑1 (MQ=255)
                                                                   gTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTa                                                                               >  2:119438/1‑150 (MQ=255)
                                                                   gTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTa                                                                               >  2:1897902/1‑150 (MQ=255)
                                                                      aGCCATTACGCTATCCGACACAGTGTTAAATCCACGCTTTTTTCCTTACCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATTTATTTTTTCCCGATTTCCCTGTTGATGTGGATGTGCAGTTAACg                                                                            >  1:249671/1‑150 (MQ=255)
                                                                       gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                           >  1:6695346/1‑150 (MQ=255)
                                                                       gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTCTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGa                                                                           <  2:4896465/150‑1 (MQ=255)
                                                                         cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGt                                                                         >  1:5757349/1‑150 (MQ=255)
                                                                         cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCACGATTTCCCTTTTGAGGTTGATGTGCAGTTATCGAGt                                                                         >  1:709715/1‑150 (MQ=255)
                                                                           ttACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGttt                                                                       >  2:7451595/1‑150 (MQ=255)
                                                                              cGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCAc                                                                    <  1:7473226/150‑1 (MQ=255)
                                                                                 tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTga                                                                 >  1:1224821/1‑150 (MQ=255)
                                                                                 tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTga                                                                 >  1:4165631/1‑150 (MQ=255)
                                                                                 tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTga                                                                 >  1:10313305/1‑150 (MQ=255)
                                                                                     cGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                             >  2:7679526/1‑150 (MQ=255)
                                                                                     cGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattac                                                             <  2:3057728/150‑1 (MQ=255)
                                                                                      gACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTgattacg                                                            <  2:2516804/150‑1 (MQ=255)
                                                                                              gtTAAATCCTCGCATTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGATTATCATCAATATAAATGTATATTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGt                                                    <  1:2662743/150‑1 (MQ=255)
                                                                                                tAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTgc                                                  <  1:614327/150‑1 (MQ=255)
                                                                                                      cTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGAt                                            <  1:7706326/150‑1 (MQ=255)
                                                                                                          cTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTAc                                        <  2:6326581/150‑1 (MQ=255)
                                                                                                           ttttttCCTTCCCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACa                                       >  2:2900576/1‑150 (MQ=255)
                                                                                                               ttccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGc                                   >  2:1387766/1‑150 (MQ=255)
                                                                                                                 ccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                                 <  2:8073803/150‑1 (MQ=255)
                                                                                                                 ccttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGt                                 <  2:2195178/150‑1 (MQ=255)
                                                                                                                  cttccCCGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTc                                >  2:6033264/1‑150 (MQ=255)
                                                                                                                      cccGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTg                            >  1:9305996/1‑150 (MQ=255)
                                                                                                                       cagAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGTTCTACATGGCGTCATTGc                           <  2:1982588/148‑1 (MQ=255)
                                                                                                                        cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGcc                          >  2:5122308/1‑150 (MQ=255)
                                                                                                                        cGAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGcc                          <  1:110134/150‑1 (MQ=255)
                                                                                                                         gAACTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCATTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCa                         <  1:10914979/150‑1 (MQ=255)
                                                                                                                           aCTGAAATAAATTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGa                       <  2:3616286/150‑1 (MQ=255)
                                                                                                                               aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAggg                   >  2:5837010/1‑150 (MQ=255)
                                                                                                                               aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAggg                   >  1:10024427/1‑150 (MQ=255)
                                                                                                                               aaataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAggg                   >  2:4157307/1‑150 (MQ=255)
                                                                                                                                aataaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGc                  >  2:1106240/1‑150 (MQ=255)
                                                                                                                                  taaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCgg                <  2:5489689/150‑1 (MQ=255)
                                                                                                                                   aaatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa               <  1:4519160/150‑1 (MQ=255)
                                                                                                                                   aaatGAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGa               <  2:8616188/150‑1 (MQ=255)
                                                                                                                                    aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGAt              >  1:9226922/1‑150 (MQ=255)
                                                                                                                                    aatTAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGAt              <  1:8862442/150‑1 (MQ=255)
                                                                                                                                       tAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc           <  1:3857874/150‑1 (MQ=255)
                                                                                                                                       tAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc           <  2:9458596/150‑1 (MQ=255)
                                                                                                                                       tAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc           <  2:1026953/150‑1 (MQ=255)
                                                                                                                                       tAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc           <  1:8970421/150‑1 (MQ=255)
                                                                                                                                       tAGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAc           <  1:7984373/150‑1 (MQ=255)
                                                                                                                                        aGCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGAcc          <  2:10804755/150‑1 (MQ=255)
                                                                                                                                         gCGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCa         <  2:3789654/150‑1 (MQ=255)
                                                                                                                                          cGACACAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGGTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAg        >  1:218263/1‑150 (MQ=255)
                                                                                                                                           gACACAGCCTGTGGCTGCTTTATCAACAATATAAATGTATTAATTCCCGATATCCATGTTGAGGTTGATGTGAATTTAACTAGTTTAACTGATTAAGGATTACGAGCTCTGAACTAAATGGCGTCATAGCCAGAAGGGCGGATGACCAGt       >  2:687680/1‑150 (MQ=255)
                                                                                                                                            acacAGCCTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTa      >  1:151067/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTC  >  NC_000913/4405982‑4406275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: