Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,633,353 A→C 5.9% D131E (GAT→GAG yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,633,3530AC 5.9% 2429.3 / 40.0 775D131E (GAT→GAGyjjXnon‑canonical purine NTP phosphatase, ITPase/XTPase
Reads supporting (aligned to +/- strand):  ref base A (392/322);  new base C (3/58);  total (395/380)
Fisher's exact test for biased strand distribution p-value = 9.20e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 827 total aligned reads displayed.

ATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTGCTGTC  >  NC_000913/4633204‑4633502
                                                                                                                                                     |                                                                                                                                                     
aTAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtca                                                                                                                                                       <  1:4568500/150‑1 (MQ=255)
 tAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcat                                                                                                                                                      <  2:1072835/150‑1 (MQ=255)
  aaGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcatc                                                                                                                                                     <  1:6855951/150‑1 (MQ=255)
  aaGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCtcatc                                                                                                                                                     >  2:3761064/1‑150 (MQ=255)
        cgGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATAcc                                                                                                                                               >  2:4030142/1‑150 (MQ=255)
         ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCg                                                                                                                                              <  2:961529/150‑1 (MQ=255)
         ggCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGCACTCAGCGCCAGGATCACCGCATGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACTCCGATCGCCCCTACCTTACGACCAATCTCATCAATACCg                                                                                                                                              >  2:3634376/1‑150 (MQ=255)
          gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCgg                                                                                                                                             >  2:2844850/1‑150 (MQ=255)
          gCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCgg                                                                                                                                             <  2:300382/150‑1 (MQ=255)
                tCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTACAAc                                                                                                                                       >  1:2199314/1‑150 (MQ=255)
                 cGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAAcg                                                                                                                                      >  1:9079194/1‑150 (MQ=255)
                    aTCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCAGCAATACCGGTATAACGCGa                                                                                                                                   <  1:3392673/150‑1 (MQ=255)
                     tCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGAc                                                                                                                                  <  2:4082774/150‑1 (MQ=255)
                      cAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACa                                                                                                                                 <  1:8033074/150‑1 (MQ=255)
                      cAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACa                                                                                                                                 <  1:8394354/150‑1 (MQ=255)
                       aGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACAt                                                                                                                                >  1:728736/1‑150 (MQ=255)
                                aTACACGGCATTATGAAACGGACACAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGACTGAGTTGCCCGGCGGGAAACACGCCGATCGCCCCTTCCTCACGACCAATCTCATCAATCCCGGTATCACGCGACATCACGGGAcc                                                                                                                       >  2:11086263/1‑150 (MQ=255)
                                 tACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCa                                                                                                                      <  1:5923798/150‑1 (MQ=255)
                                    acGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCACTCTCATCACTACCGGTATAACGCGACATCACGGGACCAAgc                                                                                                                   >  1:10522912/1‑150 (MQ=255)
                                    acGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAgc                                                                                                                   >  1:5041886/1‑150 (MQ=255)
                                    acGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAgc                                                                                                                   >  2:1873758/1‑150 (MQ=255)
                                      ggCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAgcgc                                                                                                                 <  2:7004827/150‑1 (MQ=255)
                                            aTGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCAcc                                                                                                           <  2:3240324/150‑1 (MQ=255)
                                             tGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGGTCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCt                                                                                                          >  1:4199135/1‑150 (MQ=255)
                                             tGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCt                                                                                                          >  1:194477/1‑150 (MQ=255)
                                             tGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCt                                                                                                          >  2:4179744/1‑150 (MQ=255)
                                              gAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCCTCAATACCGGTATAACGCGGCATCACGGGACCAAGCGCCTCACCtt                                                                                                         <  1:6239625/150‑1 (MQ=255)
                                              gAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCtt                                                                                                         <  2:10495443/150‑1 (MQ=255)
                                               aaaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCAGCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTc                                                                                                        >  1:1678858/1‑150 (MQ=255)
                                               aaaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCGTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTc                                                                                                        >  2:5802374/1‑150 (MQ=255)
                                                aaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGTTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCa                                                                                                       <  1:5141431/150‑1 (MQ=255)
                                                aaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATGACGCGACATCACGGGACCAAGCGCCTCACCTTCa                                                                                                       <  2:6871802/150‑1 (MQ=255)
                                                aaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCa                                                                                                       <  2:7326499/150‑1 (MQ=255)
                                                aaCGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCa                                                                                                       <  2:9847041/150‑1 (MQ=255)
                                                  cGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACg                                                                                                     >  1:9770149/1‑150 (MQ=255)
                                                   ggACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGc                                                                                                    >  1:6504872/1‑150 (MQ=255)
                                                     aCTCAGCGCCTGGATCACCGCCTGGTTATTGACGCTGGCGCGAGTGTGTTTCCCGGGGGTAAACACGCCGATCGCCCCTTCCTTACGACCTATCTCCTCAATACGGGTATACGGCGTAATCCCGGGACCAAGCGCCTCACCTTCACGCAc                                                                                                  <  1:4309491/150‑1 (MQ=255)
                                                     aCTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCAc                                                                                                  >  1:607449/1‑150 (MQ=255)
                                                       tCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACtt                                                                                                >  1:10109194/1‑150 (MQ=255)
                                                         aGCGCCAGGGTCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCACTACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACtttt                                                                                              >  1:299842/1‑150 (MQ=255)
                                                         aGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACtttt                                                                                              >  1:10369027/1‑150 (MQ=255)
                                                         aGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACtttt                                                                                              <  2:8295366/150‑1 (MQ=255)
                                                          gcgcCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACttttt                                                                                             >  2:4762875/1‑150 (MQ=255)
                                                          gcgcCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACttttt                                                                                             <  2:1232664/150‑1 (MQ=255)
                                                          gcgcCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACttttt                                                                                             >  1:9961053/1‑150 (MQ=255)
                                                          gcgcCAGGATCACCGCCTGGTGATAGACGCTGGAGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCATGGGACCAAGCGCCTCACCTTCACGCACttttt                                                                                             <  2:6569321/150‑1 (MQ=255)
                                                            gcCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCACTCTCATCCATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTcc                                                                                           >  1:8798648/1‑150 (MQ=255)
                                                            gcCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTcc                                                                                           >  2:2393918/1‑150 (MQ=255)
                                                             ccAGGCTCACCGCCTGGTGATAGACGCGGGCGCGAGTGAGTTTCCCGTCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCa                                                                                          <  2:6052204/150‑1 (MQ=255)
                                                               aGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGa                                                                                        <  1:8871010/150‑1 (MQ=255)
                                                               aGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGa                                                                                        >  2:5960762/1‑150 (MQ=255)
                                                               aGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGa                                                                                        <  1:4824040/150‑1 (MQ=255)
                                                                      ccGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCg                                                                                 <  2:4912446/150‑1 (MQ=255)
                                                                       cGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACTCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGc                                                                                <  2:10291898/150‑1 (MQ=255)
                                                                       cGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCCTCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGc                                                                                <  1:5380394/150‑1 (MQ=255)
                                                                       cGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGc                                                                                >  2:5333005/1‑150 (MQ=255)
                                                                        gCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCa                                                                               <  2:9143696/150‑1 (MQ=255)
                                                                            ggTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCa                                                                           >  1:7507723/1‑150 (MQ=255)
                                                                             gTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCaa                                                                          >  2:5653695/1‑150 (MQ=255)
                                                                               gATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACg                                                                        <  2:165668/150‑1 (MQ=255)
                                                                               gATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACg                                                                        <  1:9209677/150‑1 (MQ=255)
                                                                               gATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACg                                                                        <  2:8560055/150‑1 (MQ=255)
                                                                                aTAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACgg                                                                       >  1:2607603/1‑150 (MQ=255)
                                                                                aTAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACgg                                                                       <  2:932893/150‑1 (MQ=255)
                                                                                aTAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACgg                                                                       >  2:9046299/1‑150 (MQ=255)
                                                                                 tAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCCTCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGc                                                                      <  1:5485351/150‑1 (MQ=255)
                                                                                   gACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCaa                                                                    >  2:6283583/1‑150 (MQ=255)
                                                                                   gACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCaa                                                                    >  2:2673435/1‑150 (MQ=255)
                                                                                      gCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGt                                                                 >  2:8854466/1‑150 (MQ=255)
                                                                                          gcgcGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGcc                                                             >  1:10748995/1‑150 (MQ=255)
                                                                                             cgAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTACAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGaa                                                          <  2:2199314/150‑1 (MQ=255)
                                                                                              gagtgagtTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAAc                                                         <  1:7847375/150‑1 (MQ=255)
                                                                                                gtgagtTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAAcgc                                                       >  2:9440163/1‑150 (MQ=255)
                                                                                                  gagtTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACgcgc                                                     <  2:4590924/150‑1 (MQ=255)
                                                                                                          cggcggTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTcgccgc                                             >  1:3895727/1‑150 (MQ=255)
                                                                                                          cggcggTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTcgccgc                                             >  1:7021833/1‑150 (MQ=255)
                                                                                                            gcggTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCCTCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCgcgc                                           <  1:9480656/150‑1 (MQ=255)
                                                                                                            gcggTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCgcgc                                           <  1:4084407/150‑1 (MQ=255)
                                                                                                                     acGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCg                                  >  2:4342491/1‑150 (MQ=255)
                                                                                                                        ccGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATACCGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGttt                               <  1:5743226/150‑1 (MQ=255)
                                                                                                                        ccGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACAGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCACTGGCTGTCGttt                               <  2:6818437/150‑1 (MQ=255)
                                                                                                                          gATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTc                             >  1:1259892/1‑150 (MQ=255)
                                                                                                                          gATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTc                             <  2:7959245/150‑1 (MQ=255)
                                                                                                                           aTCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCa                            <  1:10010425/150‑1 (MQ=255)
                                                                                                                           aTCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCa                            >  1:1428654/1‑150 (MQ=255)
                                                                                                                             cGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAAt                          <  2:1493499/150‑1 (MQ=255)
                                                                                                                                cccTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATcac                       >  1:11087149/1‑150 (MQ=255)
                                                                                                                                cccTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATcac                       >  1:2851962/1‑150 (MQ=255)
                                                                                                                                 ccttccttACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATcacc                      <  1:9644429/150‑1 (MQ=255)
                                                                                                                                   ttccttACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATcaccac                    >  2:2474178/1‑150 (MQ=255)
                                                                                                                                   ttccttACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATcaccac                    >  2:5038555/1‑150 (MQ=255)
                                                                                                                                       ttACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCaa                >  2:8099561/1‑150 (MQ=255)
                                                                                                                                        tACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAAc               >  1:6691783/1‑150 (MQ=255)
                                                                                                                                          cGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTg             <  2:10924252/150‑1 (MQ=255)
                                                                                                                                           gACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGa            >  1:10910768/1‑150 (MQ=255)
                                                                                                                                                aTCTCCTCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTg       <  1:8744876/150‑1 (MQ=255)
                                                                                                                                                aTCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGtttt                              <  1:4732790/127‑1 (MQ=255)
                                                                                                                                                aTCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTg       <  2:7203127/150‑1 (MQ=255)
                                                                                                                                                aTCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTg       >  1:3573370/1‑150 (MQ=255)
                                                                                                                                                   tcatcaATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTGCTg    >  2:7601247/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATAAGACGCGGCAGCGTCGCATCAGGCGCTTAATACACGGCATTATGAAACGGACTCAGCGCCAGGATCACCGCCTGGTGATAGACGCTGGCGCGAGTGAGTTTCCCGGCGGTAAACACGCCGATCGCCCCTTCCTTACGACCAATCTCATCAATACCGGTATAACGCGACATCACGGGACCAAGCGCCTCACCTTCACGCACTTTTTCCAGAATCACCGCAGGCAACGGCAAAGTAGCCGAACGCGCCTCGCCGCGCTGGCTGGCGTTTTCAATCACCACCCAACTGAAAGTGCTGTC  >  NC_000913/4633204‑4633502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: